- MeSH
- Contraception utilization MeSH
- Humans MeSH
- Adolescent MeSH
- Puberty physiology MeSH
- Vital Statistics MeSH
- Pregnancy, Unwanted statistics & numerical data MeSH
- Check Tag
- Humans MeSH
- Adolescent MeSH
- Female MeSH
- Geographicals
- Yugoslavia MeSH
Cervical cancer is still one of the most prevalent cancers in women and a significant cause of mortality. Cytokine gene variants and socio-demographic characteristics have been reported as biomarkers for determining the cervical cancer risk in the Indian population. This study was designed to apply a machine learning-based model using these risk factors for better prognosis and prediction of cervical cancer. This study includes the dataset of cytokine gene variants, clinical and socio-demographic characteristics of normal healthy control subjects, and cervical cancer cases. Different risk factors, including demographic details and cytokine gene variants, were analysed using different machine learning approaches. Various statistical parameters were used for evaluating the proposed method. After multi-step data processing and random splitting of the dataset, machine learning methods were applied and evaluated with 5-fold cross-validation and also tested on the unseen data records of a collected dataset for proper evaluation and analysis. The proposed approaches were verified after analysing various performance metrics. The logistic regression technique achieved the highest average accuracy of 82.25% and the highest average F1-score of 82.58% among all the methods. Ridge classifiers and the Gaussian Naïve Bayes classifier achieved the highest sensitivity-85%. The ridge classifier surpasses most of the machine learning classifiers with 84.78% accuracy and 97.83% sensitivity. The risk factors analysed in this study can be taken as biomarkers in developing a cervical cancer diagnosis system. The outcomes demonstrate that the machine learning assisted analysis of cytokine gene variants and socio-demographic characteristics can be utilised effectively for predicting the risk of developing cervical cancer.
- MeSH
- Bayes Theorem MeSH
- Cytokines genetics MeSH
- Demography MeSH
- Humans MeSH
- Uterine Cervical Neoplasms * epidemiology genetics MeSH
- Machine Learning MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
OBJECTIVE: Diabetes mellitus (DM) has been one of the leading chronic diseases worldwide over past decades. The objective of the study was to identify predictors associated with health-related quality of life (HRQOL) in diabetic patients. METHODS: A cross-sectional questionnaire-based study was conducted at the General Hospital of the city of Leskovac, between June and November 2015. The Short Form-36 (SF-36) questionnaire, EuroQol-5D (EQ-5D) and EuroQol-VAS (EQ-VAS) questionnaires were used. Univariate and multivariate linear regression analyses were performed. RESULTS: The total number of patients was 285, 112 men (39.3%) and 173 women (60.7%), average age 63.92 ± 1.07 years. The results of multiple linear regression of socio-demographic characteristics in relation to dimensions of the quality of life measured by SF-36 and EQ-VAS showed that age, country (rural) life, low level of education, retirement, and poor economic status are predictors of lower quality of life. Our results showed that employment has a significant association with higher Physical Component Score (PCS), Mental Component Score (MCS) and EQ-VAS score, which can be explained with higher incomes, improved economic status and less possibility for the occurrence of depressive mood. Patients without formal education have lower QOL. Univariate multiple regression analysis of the presence of micro- and macrovascular complications of DM showed that angina pectoris, heart failure, diabetic retinopathy, and diabetic nephropathy are the most important factors affecting the quality of life in our population. After including the multivariate model, all tested complications remained statistically significant. CONCLUSION: Our results showed that both socioeconomic and chronic complications are relevant factors of HRQOL in type 1 and 2 diabetes mellitus patients. Age, rural lifestyle, retirement, lower level of education and low socioeconomic status, as well as DM complications (angina pectoris, hearth failure, diabetes nephropathy, and diabetes retinopathy) were found to be independent risk factors for the component scores of SF-36 and EQ-VAS score. Taking into consideration the results obtained, health practitioners should be aware not only of the clinical parameters of patients with DM, but also of their educational level and working status.
- MeSH
- Demography MeSH
- Diabetes Mellitus epidemiology MeSH
- Diabetes Complications epidemiology MeSH
- Quality of Life * MeSH
- Middle Aged MeSH
- Humans MeSH
- Cross-Sectional Studies MeSH
- Risk Factors MeSH
- Socioeconomic Factors MeSH
- Health Status * MeSH
- Health Surveys MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Serbia MeSH
Glyphosate is an herbicide that is used worldwide with potential environmental risks to nontarget organisms. We applied an age-stage, two-sex life table approach to assess the sublethal effects of short-term oral exposure to a glyphosate-based herbicide on the life table parameters and biocontrol potential of Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae). Aphids (Metopolophium dirhodum (Walker) (Sternorrhyncha: Aphididae)) treated with herbicide (an isopropylamine-salt of glyphosate) at low recommended, maximum recommended, and double the maximum recommended concentration for agricultural situations, and untreated controls were offered to the fourth instar of H. axyridis for 24 h. Development, consumption, and fecundity were measured daily until death. We detected minor differences in the hatching rate and mean generation time, whereas the longevity, fecundity, net reproductive rate, intrinsic rate of increase, finite rate of increase, and consumption were unaffected across treatments. We conclude that biocontrol potential of H. axyridis was not affected by acute oral intoxication by a glyphosate-based herbicide during the larval stage for 24 h under the study design.
- MeSH
- Pest Control, Biological MeSH
- Coleoptera drug effects growth & development physiology MeSH
- Glycine analogs & derivatives toxicity MeSH
- Insecticides toxicity MeSH
- Pupa drug effects growth & development physiology MeSH
- Larva drug effects growth & development physiology MeSH
- Aphids chemistry growth & development MeSH
- Nymph chemistry growth & development MeSH
- Food Chain * MeSH
- Predatory Behavior drug effects MeSH
- Animals MeSH
- Life History Traits * MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Cíl studie: Vyhodnocením základních spermiologických parametrů podle standardů WHO u potenciálních dárců spermatu přispět k současné diskusi o mužském faktoru neplodnosti. Typ studie: Retrospektivní demografická studie. Název a sídlo pracoviště: Gyn.-por. klinika LF UP a FN Olomouc, Ústav biologie LF UP Olomouc. Metodika: V období leden 2000 – duben 2004 se dostavilo ke vstupnímu vyšetření celkem 108 potenciálních dárců spermií ve věku 18–35 let. Spermiologické vyšetření čerstvého semene získaného masturbací bylo provedeno podle standardů WHO. Vyhodnocovanými parametry byly objem ejakulátu, koncentrace spermií a celková motilita. Výsledky: Normospermie byla zjištěna jen u 46 mužů (42,6 %). U tří mužů (2,8 %) byla prokázána azoospermie. Nejčastějším patologickým nálezem byla astenospermie, která byla zjištěna u 49 (46,6 %) mužů. Závěr: Na základě rozboru získaných dat můžeme usuzovat na zřejmý pokles kvality spermiologických parametrů mladé české populace. Podle našeho názoru by provedení multicentrické analýzy se zaměřením na hodnocení plodnosti českých mužů bylo vysoce žádoucí.
Objective: To contribute to the contemporary discussion on the male factor infertility through the evaluation of the basic spermatological parameters in men recruiting for semen donation. Design: A retrospective demographic study. Setting: Dept. of Obstetrics and Gynaecology, Dept. of Biology, Faculty of Medicine, Palacký University, Olomouc. Methods: A total of 108 men aged 18 to 35 years came to the preliminary examination for the potential semen donors between January 2000 and April 2004. Spermatological analysis of the fresh ejaculates obtained by masturbation was performed according to WHO guidelines. The following parameters were evaluated: the volume of ejaculate, sperm concentration and total percent motility. Results: Normospermia was found in only 46 men (42.6%). Three males (2.8%) were azoospermic. The most frequent problem was asthenospermia, which was documented in 49 (46.6%) men. Conclusion: The analysis of the obtained data may suggest a marked semen quality decline in the Czech male population. In our opinion, further multicentric epidemiological studies concerning fertility potential in Czech male population seem highly needed.
Wild boar (Sus scrofa), one of the most widespread wildlife species, has entered a stage of continuous growth in Europe, and could even be considered a pest species. We analysed microsatellite variability in 723 wild boars from across Europe, including the northern Dinaric Balkans. Our aims were: (1) to define the population structure of wild boars in the Balkans and its relation with other European populations; (2) to estimate effective populations sizes, levels of intra- and inter-population diversity, inbreeding migration and gene flow patterns; (3) to test subpopulations for bottlenecks; (4) to interpret these results in light of current knowledge about the demographic history of wild boars in Europe; and (5) to discuss the relevance of these findings for management and conservation. Strong population structuring was observed and 14 subpopulations were revealed. High genetic diversity was found, and besides the well-known identity of the Italian populations of Sardinia and Castelporziano, we bring new insights into other potential relevant, refugial populations such as Littoral Slovenia, South Portugal, North-western Iberia and an entire cluster in the Balkans. There was evidence of gene flow going from these refugial subpopulations towards less peripheral and more admixed subpopulations. Recent population bottlenecks and expansions were detected, mostly in the peninsular refuge subpopulations. The results are consistent with the fluctuations of wild boar numbers in Europe since the beginning of the twentieth century. These results should be taken into account in future conservation and management plans for wild boar populations in Europe.
- MeSH
- Gene Frequency MeSH
- Genetic Variation * MeSH
- Genotyping Techniques MeSH
- Population Density MeSH
- Inbreeding MeSH
- Microsatellite Repeats MeSH
- Models, Genetic MeSH
- Population Dynamics MeSH
- Genetics, Population * MeSH
- Models, Statistical MeSH
- Sus scrofa genetics MeSH
- Gene Flow * MeSH
- Conservation of Natural Resources MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Balkan Peninsula MeSH
- Europe MeSH
Orius strigicollis (Poppius) is an anthocorid bug with high foraging ability on thrips as well as on mites, and the bug has been considered as a potential biological control agent in Taiwan. Life table and predation studies of O. strigicollis fed on Cadra cautella (Walker) and Tetranychus urticae (Koch) eggs were conducted at 25 ± 1°C. Data were analyzed and compared using TWOSEX-MSChart and CONSUME-MSChart software. O. strigicollis fed on eggs of C. cautella, a substitute prey, showed significantly higher survival rate and developmental rate than individuals fed on their natural prey, T. urticae eggs. The fecundity of O. strigicollis fed on C. cautella eggs was, on average, 13.2 times higher than that of those fed on T. urticae eggs, despite of the fact that during the entire nymphal stage, the consumption rate of O. strigicollis on T. urticae eggs was ca. 9 times higher than on almond moth eggs The conversion rate (i.e., number of prey eggs needed to produce one predator egg) for this predatory bug reared on T. urticae eggs and almond moth eggs were 604.6 and 6.0, respectively, indicating that almond moth eggs served as an effective alternative prey for ensuring the predator's reproduction. This is the first study pertaining to the population parameters and predation rates of O. strigicollis using the age-stage two-sex approach to describe differences between O. strigicollis populations reared on natural and alternative preys. This information may be useful in mass rearing programs and field application involving this biological control agent.
- MeSH
- Pest Control, Biological * MeSH
- Diet MeSH
- Heteroptera growth & development physiology MeSH
- Moths * growth & development MeSH
- Nymph growth & development physiology MeSH
- Ovum MeSH
- Predatory Behavior * MeSH
- Life Tables MeSH
- Tetranychidae * growth & development MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Aim: The aim of the present study was to evaluate the effects of music on preoperative state anxiety and physiological parameters including heart rate, respiratory rate and mean systolic, and diastolic blood pressure in 60 patients undergoing general surgery. Methods: The study was designed as a randomized quasi-experimental trial. A total of 60 patients were allocated to two equal groups. Subjects in the trial group listened to non-vocal classical music through headphones for 20 minutes before entering the operating theater. State anxiety was measured by the State-Trait Anxiety Inventory (STAI), and the physiological parameters by pulse CO-oximeter (Masimo RAD-57C, Finland). The results obtained were finally analyzed by SPSS software version 16 using chi-square and t-tests. Results: There was an insignificant difference in demographic data between the two groups (p > 0.05), but before and after listening to music, a significant difference was observed in state anxiety (p < 0.01). Conclusions: Significant differences in both state anxiety and physiological parameters confirmed that music as a non-pharmacological intervention can be used as a complementary tool in nursing care.
- MeSH
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Monitoring, Physiologic statistics & numerical data MeSH
- Music Therapy * statistics & numerical data MeSH
- Pragmatic Clinical Trials as Topic statistics & numerical data MeSH
- Preoperative Period MeSH
- Preoperative Care * methods statistics & numerical data MeSH
- Psychological Tests statistics & numerical data MeSH
- Aged MeSH
- Anxiety * diagnosis prevention & control psychology therapy MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Evaluation Study MeSH
Český národní registr dárců dřeně (ČNRDD) byl založen v roce 1992 s cílem nalézt včas optimálního „národního“ dárce pro české pacienty, případně zajistit dárce ze zahraničí. V současnosti (k 31. 10. 2018) jeho databáze obsahuje 83 000 dárců a medián věku všech aktivních dárců v registru je 32 let. Na základě preferencí transplantačních center, tj. větší míry požadavků na mladší dárce, jakož i z ekonomických důvodů, snížil ČNRDD v roce 2009 maximální věkovou hranici vstupu do registru na 35 let. Cílem naší práce bylo zhodnotit, jak dalece se tato restriktivní kritéria a celkovou strategii náboru zaměřenou na mladé muže dařilo dodržet, zhodnotit kvantitativní i kvalitativní parametry náboru v letech 2013–2017. V letech 2013–2017 se nábor nových dárců pohyboval mezi 5 000–10 000/rok. Celkem bylo v tomto období zařazeno 36 720 dárců. Zastoupení pohlaví mezi nově registrovanými dárci je v posledních letech téměř vyrovnané. Medián věku registrovaných dárců v celém sledovaném období byl 27 let. Tento masivní nábor mladých dárců vedl k průběžnému snížení mediánu věku dárců celé databáze ČNRDD. Prezentované údaje jednoznačně dokládají efektivitu aktuální náborové strategie ČNRDD, protože v uplynulých letech došlo k výraznému snížení mediánu věku dárců celé databáze ČNRDD a současně nárůstu podílu mužů. To je v souladu s preferencí transplantačních center, která na podkladě publikovaných dat upřednostňují mladé dárce, a to především muže. Má-li být současná náborová strategie ČNRDD nadále úspěšná, je nezbytná trvající dobrá spolupráce dárcovských a náběrových center registru i práce dobrovolných spolupracovníků.
The Czech National Marrow Donors Registry (CNMDR) was established in 1992 to find an optimal „national" donor for Czech patients or to provide a donor from abroad as soon as possible. The current state of the CNMDR database (to October 31, 2018) stands at 83.000 donors and the median age of all active donors in the registry is 32 years. Given the transplant centres' preference for younger donors and for economic reasons, the CNMDR reduced the maximum age limit for entry into the registry to 35 years in 2009. The aim of our work was to evaluate the success of this age restriction and general recruitment strategy focusing on young males and to evaluate the quantitative and qualitative recruitment parameters in 2013–2017. Between 2013 and 2017, the recruitment of new donors ranged from 5.000 to 10.000/year. In total, 36.720 donors were recruited during this period. Newly recruited donor gender distribution has been almost equally balanced in recent years. The average median age of recruited donors was 27 years. This massive recruitment of young donors led to a continuous reduction of the CNMDR database donor median age. The presented data clearly demonstrate the effectiveness of the current CNMDR recruitme strategy. In the past years, there has been a significant decrease in the donor age median as well as an increase in male donor contribution. This is in accordance with the preferences of transplant centres that prefer young donors especially males, based on published data. For this recruitment strategy to remain successful, the CNMDR will need to maintain its continued good cooperation with donor and recruitment centres as well as with volunteers.
- Keywords
- nábor,
- MeSH
- Tissue Donors * statistics & numerical data MeSH
- Adult MeSH
- Bone Marrow MeSH
- Humans MeSH
- Young Adult MeSH
- Registries MeSH
- Donor Selection statistics & numerical data MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Young Adult MeSH
- Male MeSH
- Female MeSH
- Geographicals
- Czech Republic MeSH
BACKGROUND: The presence of gastric ectopic mucosa in Meckel's diverticulum is associated with a higher risk of development of complications. The aim of the present study was to investigate which demographic/clinical parameters predict the presence of gastric heterotopia in Meckel's diverticulum. METHODS: This was a retrospective cohort study conducted in a single institution (University Hospital Ostrava, Czech republic). All children who underwent laparoscopic/open resection of Meckel's diverticulum within a 20-year study period were included in the study. RESULTS: In total, 88 pediatric patients underwent analysis. The mean age of the children was 4.6 ± 4.73 years; the male-female ratio was approximately 2:1. There were 50 (56.8%) patients with asymptomatic Meckel's diverticulum in our study group. Laparoscopic resection was performed in 24 (27.3%) patients; segmental bowel resection through laparotomy was performed in 13 (14.8%) patients. Gastric heterotopia was found in 39 (44.3%) patients; resection margins of all patients were clear of gastric heterotopia. No correlation was found between the presence of gastric heterotopia and the following parameters: age, gender, maternal age, prematurity, low birth weight, perinatal asphyxia, distance from Bauhin's valve and length of Meckel's diverticulum. The width of the diverticulum base was significantly higher in patients with gastric heterotopia (2.1 ± 0.57 vs. 1.2 ± 0.41 cm; p < 0.001). CONCLUSIONS: According to the study outcomes, the width of the diverticulum base seems to be a significant predictive factor associated with the presence of gastric heterotopia in Meckel's diverticulum. The laparoscopic/open resection of asymptomatic MD with a wide base should therefore be recommended.
- MeSH
- Choristoma pathology surgery MeSH
- Child MeSH
- Cohort Studies MeSH
- Infant MeSH
- Laparoscopy MeSH
- Laparotomy MeSH
- Humans MeSH
- Meckel Diverticulum pathology surgery MeSH
- Adolescent MeSH
- Child, Preschool MeSH
- Retrospective Studies MeSH
- Gastric Mucosa * MeSH
- Check Tag
- Child MeSH
- Infant MeSH
- Humans MeSH
- Adolescent MeSH
- Male MeSH
- Child, Preschool MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
