elektronický časopis
- Conspectus
- Experimentální medicína
- NML Fields
- experimentální medicína
- onkologie
- NML Publication type
- elektronické časopisy
sv.
- MeSH
- Neoplasms, Experimental MeSH
- Neoplasms MeSH
- Publication type
- Periodical MeSH
- Conspectus
- Patologie. Klinická medicína
- NML Fields
- onkologie
- experimentální medicína
Advances in experimental medicine and biology ; vol. 176
505 s. : il.
The human colorectal carcinoma (CRC)-associated Ag CO17-1A/GA733, originally defined by mAbs CO17-1A and GA733, has been a useful target in passive immunotherapy of CRC patients with mAb and in active immunotherapy with anti-idiotypic Abs mimicking the CO17-1A or GA733 epitope. Both approaches have targeted single epitopes. We investigated the capacity of full-length CO17-1A/GA733 Ag expressing multiple potentially immunogenic epitopes and encoded by recombinant adenovirus 5 (Ad5 GA733-2) to induce humoral, cellular, and/or protective immunity in mice. Ad5 GA733-2 induced Ag-specific Abs that reacted predominantly to CO17-1A- and GA733-unrelated epitopes on the Ag and lysed Ag-positive CRC targets in conjunction with effector cells. Ad5 GA733-2-immune mice developed Ag-specific, proliferative lymphocytes of Th1 type and cytolytic lymphocytes. The use of Ad5 GA733-2 to immunize mice bearing established syngeneic CRC cells transfected with the human Ag induced significant and specific tumor regression. Cured mice resisted rechallenge with human CO17-1A/GA733 Ag-negative parental CRC cells, suggesting that targeting the human Ag on the murine transfectants induced protective immunity to other Ag expressed by the parental tumor. These results may explain the high potency of the recombinant vaccine. Thus, rAd5 GA733-2 may have potential as a vaccine for CRC patients.
- MeSH
- Adenoviridae * immunology MeSH
- Antigens, Neoplasm * genetics immunology MeSH
- Antigens, Viral genetics immunology MeSH
- Cell Division genetics immunology MeSH
- Neoplasms, Experimental genetics MeSH
- Carcinoma genetics immunology pathology MeSH
- Colorectal Neoplasms genetics immunology pathology MeSH
- Humans MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Gene Expression Regulation, Neoplastic * immunology MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
26 sv.
- MeSH
- Neoplasms, Experimental MeSH
- Medical Oncology MeSH
- Neoplasms MeSH
- Investigative Techniques MeSH
- Publication type
- Periodical MeSH
- Conspectus
- Experimentální medicína
- NML Fields
- onkologie
- experimentální medicína
