UWBC biotechnical resource series
XV, 279 s. : il. ; 24 cm
1st ed. xiv, 286 s.
elektronický časopis
- Conspectus
- Obecná genetika. Obecná cytogenetika. Evoluce
- NML Fields
- biologie
- genetika, lékařská genetika
- NML Publication type
- elektronické časopisy
1 online zdroj
- MeSH
- DNA, Mitochondrial * MeSH
- Sequence Analysis, DNA MeSH
- Publication type
- Periodical MeSH
- Conspectus
- Biochemie. Molekulární biologie. Biofyzika
- NML Fields
- genetika, lékařská genetika
The biosynthetic gene cluster of porothramycin, a sequence-selective DNA alkylating compound, was identified in the genome of producing strain Streptomyces albus subsp. albus (ATCC 39897) and sequentially characterized. A 39.7 kb long DNA region contains 27 putative genes, 18 of them revealing high similarity with homologous genes from biosynthetic gene cluster of closely related pyrrolobenzodiazepine (PBD) compound anthramycin. However, considering the structures of both compounds, the number of differences in the gene composition of compared biosynthetic gene clusters was unexpectedly high, indicating participation of alternative enzymes in biosynthesis of both porothramycin precursors, anthranilate, and branched L-proline derivative. Based on the sequence analysis of putative NRPS modules Por20 and Por21, we suppose that in porothramycin biosynthesis, the methylation of anthranilate unit occurs prior to the condensation reaction, while modifications of branched proline derivative, oxidation, and dimethylation of the side chain occur on already condensed PBD core. Corresponding two specific methyltransferase encoding genes por26 and por25 were identified in the porothramycin gene cluster. Surprisingly, also methyltransferase gene por18 homologous to orf19 from anthramycin biosynthesis was detected in porothramycin gene cluster even though the appropriate biosynthetic step is missing, as suggested by ultra high-performance liquid chromatography-diode array detection-mass spectrometry (UHPLC-DAD-MS) analysis of the product in the S. albus culture broth.
- MeSH
- Anthramycin analogs & derivatives biosynthesis chemistry MeSH
- Bacterial Proteins genetics metabolism MeSH
- Molecular Sequence Data MeSH
- Molecular Structure MeSH
- Multigene Family * MeSH
- Sequence Analysis MeSH
- Streptomyces chemistry genetics metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
... Using Protein Sequences, 197 -- 9 Protein Structure Prediction and Analysis, 223 -- I 0 Intermolecular ... ... , 295 -- I 2 Creation and Analysis of Protein Multiple Sequence Alignments, 325 -- I 3 Sequence Assembly ... ... Wolfsberg -- PART TWO -- ANALYSIS AT THE NUCLEOTIDE LEVEL -- 5 Predictive Methods Using DNA Sequences ... ... Using Protein Sequences, 197 -- Yanay Ofran and Burkhard Rost -- 9 Protein Structure Prediction and Analysis ... ... Baxevanis -- I 2 Creation and Analysis of Protein Multiple Sequence Alignments, 325 -- Geoffrey J. ...
3rd ed. xviii, 540 s. : il. ; 29 cm
- MeSH
- Base Sequence methods MeSH
- Sequence Analysis, Protein methods MeSH
- Computational Biology methods MeSH
- Conspectus
- Biochemie. Molekulární biologie. Biofyzika
- NML Fields
- biochemie
2nd ed. x, 193 s., il.
