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Progress in mutation research ; Vol. 3
364s. : il.
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- embryologie a teratologie
- genetika, lékařská genetika
Adipose/fat tissue provides an abundant source of stromal vascular fraction (SVF) cells for immediate administration and can also give rise to a substantial number of cultured, multipotent adipose-derived stromal cells (ADSCs). Recently, both SVF and ADSCs have gained wide-ranging translational significance in regenerative medicine. Initially used for cosmetic breast enhancement, this mode of treatment has found use in many diseases involving immune disorders, tissue degeneration, and ischaemic conditions. In this review, we try to address several important aspects of this field, outlining the biology, technology, translation, and challenges related to SVF- and ADSC-based therapies. Starting from the basics of SVF and ADSC isolation, we touch upon recently developed technologies, addressing elements of novel methods and devices under development for point-of-care isolation of SVF. Characterisation of SVF cells and ADSCs is also an evolving area and we look into unusual expression of CD34 antigen as an interesting marker for such purposes. Based on reports involving different cells of the SVF, we draw a potential mode of action, focussing on angiogenesis since it involves multiple cells, unlike immunomodulation which is governed predominantly by ADSCs. We have looked into the latest research, experimental therapies, and clinical trials which are utilising SVF/ADSCs in conditions such as multiple sclerosis, Crohn's disease, peripheral neuropathy, osteoarthritis, diabetic foot ulcer, and so forth. However, problems have arisen with regards to the lack of proper regulatory guidelines for such therapies and, since the introduction of US Food and Drug Administration draft guidelines and the Reliable and Effective Growth for Regenerative Health Options that Improve Wellness (REGROW) Act, the debate became more public with regards to safe and efficacious use of these cells.
- MeSH
- buňky stromatu metabolismus patologie transplantace MeSH
- Crohnova nemoc metabolismus patologie terapie MeSH
- diabetická noha metabolismus patologie terapie MeSH
- lidé MeSH
- multipotentní kmenové buňky metabolismus patologie transplantace MeSH
- nemoci periferního nervového systému metabolismus patologie terapie MeSH
- osteoartróza metabolismus patologie terapie MeSH
- regenerativní lékařství metody MeSH
- roztroušená skleróza metabolismus patologie terapie MeSH
- tuková tkáň metabolismus patologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
Microbial entomopathogens that include fungi, bacteria, viruses, and nematodes have long been valued for their role in biological control of insect pests. However, recent research highlights their expanded applications beyond pest management. Entomopathogenic fungi such as Beauveria bassiana and Metarhizium spp. are increasingly recognized for their potential as biocontrol agents in integrated pest management systems. These fungi exhibit not only direct insecticidal effects but also secondary metabolites that contribute to plant disease suppression, thereby enhancing crop health and yield. Bacterial entomopathogen Bacillus thuringiensis, as the most widely used biopesticide, has also demonstrated potency not only against insects but also as systemic resistance inducer, thereby boosting plant immunity against pathogens. Moreover, entomopathogens are emerging as growth promoters and biostimulants, enhancing crop vigor through nutrient uptake and root development. This review consolidates current knowledge on the mechanisms of action of microbial entomopathogens against pests as well as current understanding on its other plant-beneficial traits. It also discusses their environmental impact and potential integration into sustainable agricultural practices. This comprehensive exploration underscores the transformative potential of microbial entomopathogens in shaping future strategies for holistic crop health management including pest management in agriculture.
- MeSH
- Bacillus thuringiensis MeSH
- Bacteria MeSH
- Beauveria MeSH
- biologická kontrola škůdců * metody MeSH
- biologická ochrana * MeSH
- hmyz mikrobiologie MeSH
- houby * MeSH
- Metarhizium fyziologie MeSH
- nemoci rostlin prevence a kontrola parazitologie mikrobiologie MeSH
- zemědělské plodiny mikrobiologie růst a vývoj MeSH
- zemědělství * metody MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
3rd ed. x, 610 s. : tab. ; 24 cm
INTRODUCTION: Sepsis-induced acute kidney injury (AKI) remains a major challenge in intensive care, contributing significantly to morbidity and mortality. Tibolone, known for its neuroprotective and hormonal properties, has not been explored for its potential in AKI management. This study investigates the protective effects of Tibolone and its underlying mechanisms involving Sirtuin-1 (SIRT1) and Yes-Associated Protein (YAP) in a rat sepsis model. MATERIALS AND METHODS: Thirty-six female Wistar albino rats underwent cecal ligation and puncture (CLP) to induce sepsis. They were randomly assigned to control, CLP+Saline, and CLP+Tibolone groups. Tibolone was administered intraperitoneally. Biomarkers, including Sirtuin (SIRT1), Yes-associated protein (YAP), Tumor necrosis factor (TNF-α), High mobility group box 1 (HMGB1), malondialdehyde (MDA), creatinine, and urea, were assessed. Histopathological examination evaluated renal damage. RESULTS: Tibolone administration significantly reduced plasma TNF-α, HMGB1, MDA, creatinine, and urea levels compared to the CLP+Saline group. Moreover, Tibolone elevated SIRT1 and YAP levels in kidney tissues. Histopathological examination demonstrated a significant decrease in tubular epithelial necrosis, luminal debris, dilatation, hemorrhage, and interstitial inflammation in Tibolone-treated rats. CONCLUSION: This study unveils the protective role of Tibolone against sepsis-induced AKI in rats. The improvements in inflammatory and oxidative biomarkers and histological evidence suggest Tibolone's potential as a therapeutic intervention in sepsis-associated kidney injury. The upregulation of SIRT1 and YAP indicates their involvement in Tibolone's renoprotective mechanisms. Further investigations are warranted to explore Tibolone's translational potential in human sepsis-induced AKI.
- MeSH
- akutní poškození ledvin * etiologie prevence a kontrola farmakoterapie MeSH
- biologické markery MeSH
- krysa rodu rattus MeSH
- ledviny účinky léků metabolismus MeSH
- modely nemocí na zvířatech MeSH
- norpregneny * terapeutické užití farmakologie MeSH
- potkani Wistar * MeSH
- protein HMGB1 metabolismus MeSH
- sepse * komplikace farmakoterapie MeSH
- sirtuin 1 * metabolismus MeSH
- TNF-alfa metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Carotid body paragangliomas (CBP) are rare neoplasms arising from the small chemoreceptor organ in the adventitia of the common carotid bifurcation. The aim of this study is to present the diagnostic process, performed treatment and obtained results in patients with carotid body paragangliomas of the Department of Otolaryngology of Istanbul Education and Research Hospital between March 1997 and November 2008. Retrospective analysis was carried out, based on the medical documentation of 5 patients with carotid body paragangliomas (3 women and 2 men), age range 44 to 68 years with a mean of 59.6 years. Four of the patients were diagnosed and treated with Shamblin type II tumor, one of the patients with type I. Physical examination, radiological evaluation, method of the treatment and post-treatment complications were studied. The most common and single symptom was nonspecific neck mass. Preoperative diagnostic evaluation consisted of a color duplex ultrasonography, computerized tomography with contrast enhancement, magnetic resonance imaging and digital subtraction angiography. In all patients with Shamblin type I and II, blunt dissection of the tumor was conducted smoothly in the subadventitial plane. Postoperative vagus nerve and hypoglossal nerve deficit were reported in one case. Carotid body paraganglioma excision has higher risk of cranial nerve paresis and carotid artery injury, so it requires careful handling and good surgical skills to ensure complete removal.
- MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- senioři MeSH
- tumor karotického glomu * diagnóza chirurgie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Fifty fluorescent pseudomonads were isolated from rhizospheric soil of green gram from nearby area of Kaziranga, Assam, India and assayed for their extracellular proteinase production. Out of these isolates, 20 were found to be prominent in proteinase production. Genetic diversity of the 20 isolates were analyzed through BOX-PCR fingerprinting and 16S rDNA-RFLP along with three reference strains, viz., Pseudomonas fluorescens (NCIM2099(T)), Pseudomonas aureofaciens (NCIM2026(T)), and Pseudomonas aeruginosa (MTCC2582(T)). BOX-PCR produced two distinct clusters at 56% similarity coefficient and seven distinct BOX profiles. 16S rDNA-RFLP with three tetra-cutters restriction enzymes (HaeIII, AluI, and MspI) revealed two major clusters A and B; cluster A contained only single isolate FPS9 while the rest of 22 isolates belonged to the cluster B. Based on phenotypic characters and 16S rDNA sequence similarity, all the eight highly proteinase-producing strains were affiliated with P. aeruginosa. The proteinase was extracted from two most prominent strains (KFP1 and KFP2), purified by a three-step process involving (NH(4))(2)SO(4) precipitation, gel filtration, and ion exchange chromatography. The enzyme had an optimal pH of 8.0 and exhibit highest activity at 60°C and 37°C by KFP1 and KFP2 respectively. The specific activities were recorded as 75,050 (for KFP1) and 81,320 U/mg (for KFP2). The purified enzyme was migrated as a single band on native and SDS-PAGE with a molecular mass of 32 kDa. Zn(2+), Cu(2+), and Ni(2+) ion inhibited the enzyme activity. Enzyme activity was also inhibited by EDTA established as their metallo-proteinase nature.
- MeSH
- bakteriální proteiny genetika metabolismus MeSH
- endopeptidasy genetika metabolismus MeSH
- Fabaceae mikrobiologie MeSH
- fylogeneze MeSH
- molekulární sekvence - údaje MeSH
- Pseudomonas klasifikace enzymologie genetika izolace a purifikace MeSH
- půdní mikrobiologie MeSH
- rhizosféra MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Indie MeSH
