isolation methods
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Mammalian adipose tissue derived stem cells (AT-SC) have a tremendous potential in regenerative medicine for tissue engineering and somatic nuclear transfer (SNT). The isolation methods of human and bovine adipose tissue derived stem cells are compared in this paper to determine the feasibility and optimum method of isolation. The optimum isolation method will reduce the processing time, efforts and money as isolation is the first crucial and important step in stem cells research. Human abdominal subcutaneous adipose tissue and bovine abdominal subcutaneous adipose tissue are digested in three collagenase type 1 concentration 0.075%, 0.3% and 0.6% agitated at 1 h and 2 h under 37 °C in 5% CO2 incubator. The cultures are then morphologically characterised. Human adipose tissue stem cells are found to be best isolated using abdominal subcutaneous depot, using 0.075% collagenase type 1 agitated at 1 h under 37 °C in CO2 incubator. While bovine adipose tissue derived stem cells are best isolated using abdominal subcutaneous depot, using 0.6% collagenase type 1 agitated at 2 h under 37 °C in CO2 incubator.
- MeSH
- buněčná diferenciace MeSH
- buněčné kultury metody MeSH
- druhová specificita MeSH
- kmenové buňky cytologie fyziologie MeSH
- kultivované buňky MeSH
- lidé MeSH
- separace buněk metody MeSH
- skot MeSH
- tkáňové inženýrství metody MeSH
- tukové buňky cytologie fyziologie MeSH
- viabilita buněk fyziologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
- srovnávací studie MeSH
Pozadí: Izolace infekčních nebo imunosuprimovaných pacientů je běžnou intervencí v nemocnicích. Důkazy zdůrazňují různé dopady v důsledku izolace. Cíl: Prozkoumat zkušenosti dospělých hospitalizovaných pacientů s izolací na jednolůžkovém pokoji. Metodika: Byla provedena integrační revize. Proto bylo provedeno systematické vyhledávání v Cochrane, CINAHL, MEDLINE a GeroLit, stejně jako dodatečné ruční vyhledávání a zpětné vyhledávání v letech 2021 a 2023. Screening a extrakci dat prováděli nezávisle dva recenzenti. Syntéza výsledků studie byla provedena konstantním porovnáváním. Výsledky: Z 5 975 zjištění bylo zahrnuto 20 studií (9 kvalitativních, 9 kvantitativních, 2 smíšené) obsahujících 58 534 účastníků. Zkušenosti pacientů závisí na různých kontextových faktorech. Studie popisují mnohostrannou zkušenost s izolací na jednolůžkovém pokoji. Izolace je vnímána jako ochrana před hrozbami; pacienti zažívají klid a soukromí, ale jsou také konfrontováni s omezeními ve vztahu k mobilitě a kontaktu. Studie zmiňují, že izolace má různé psychologické a sociální dopady a také vliv na vnímání poskytované péče. Ačkoli je to často vnímáno jako velká zátěž, studie zdůrazňují příležitost k sebereflexi a nalezení vnitřní síly. Diskuse: Pacienti uváděli komplexní, multidimenzionální a nekonzistentní zkušenost. Je zapotřebí dalšího výzkumu, aby se řešily diferencované kauzální vztahy mezi ovlivňujícími faktory a různými zkušenostmi.
Background: Isolation of infectious or immunosuppressed patients is a common intervention in hospitals. Evidence highlights various impacts due to isolation. Aim: To explore adult inpatients' experience of single room isolation. Methods: An integrative review was performed. Therefore, a systematic search was conducted in Cochrane, CINAHL, MEDLINE, and GeroLit, as well as an additional handsearch and a reverse search in 2021 and 2023. Screening and data extraction were carried out independently by two reviewers. Synthesis of study results was performed by constant comparison. Results: Of the 5,975 findings, 20 studies (9 qualitative, 9 quantitative, 2 mixed) containing 58,534 participants were included. Patients' experience depends on various context factors. The studies describe a multifaceted experience of single room isolation. Isolation is perceived as a protection against threats; patients experience peace and privacy but are also confronted with restrictions in relation to mobility and contact. The studies mention that isolation has various psychological and social impacts, as well as effects on the perception of received care. Although often perceived as a heavy burden, the studies highlight the opportunity for self-reflection and to find inner strength. Discussion: Patients reported a complex, multidimensional, and inconsistent experience. Further research is needed to address differentiated causal relationships between the influencing factors and different experiences.
... Methods for isolating RNA 15 -- Basic principles 15 -- Cell lysis and fractionation procedures 17 -- ... ... Isolation of total RNA by cell lysis 17 -- Isolation of RNA from cell fractions 19 -- Isolation of RNA ... ... using phenol extraction methods 23 -- Isolation of total RNA by acid guanidinium thiocyanate methods ... ... 27 -- Isolation of total cellular RNA using the guanidinium-lithium chloride method 28 vi RNA ISOLATION ... ... -- Fractionation of RNA using magnetic beads 37 -- Isolation of newly synthesized RNA by affinity methods ...
xi, 196 stran : ilustrace, tabulky ; 24 cm
V práci sa referuje o ojedinelom prípade izolácie Neisseria meningitidis C:2a:P1.2,P1.5 v auguste1997 od 7-ročnej pacientky z výteru z pošvy pri purulentnom zápale vonkajšieho genitálu po úraze.Ide o prvý popísaný prípad izolácie N. meningitidis z takejto lokalizácie u nás a upozorňuje nanutnosť myslieť na neobvyklý výskyt N. meningitidis, vyšetrovať atypické materiály aj mikroskopic-ky a všetky izolované neisérie vrátane gonokokov identifikovať aj biochemicky.
The authors describe a unique case of isolation of Neisseria meningitidis C:2a:P1.2,P1.5 in August1997 from a 7-year-old female patient from a vaginal smear during purulent inflammation of theexternal genital following injury. This is the first described case of isolation of N. meningitidis fromthis site described in our country. The authors draw attention to the necessity to consider uncom-mon occurrences of N. meningitidis, and to examine atypical materials also under the microscopeand to identify by biochemical methods all isolated Neisseriae incl. gonococci.
- MeSH
- dítě MeSH
- lidé MeSH
- Neisseria meningitidis izolace a purifikace MeSH
- vagina zranění MeSH
- vaginitida mikrobiologie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- Publikační typ
- kazuistiky MeSH
Soil microbial populations play crucial role in soil properties and influence below-ground ecosystem processes. Microbial composition and functioning changes the soil quality through decomposition of organic matter, recycling of nutrients, and biological control of parasites of plants. Moreover, the discovery that soil microbes may translate into benefits for biotechnology, management of agricultural, forest, and natural ecosystems, biodegradation of pollutants, and waste treatment systems maximized the need of scientists for the isolation and their characterization. Operations such as the production of antibiotics and enzymic activities from microorganisms of soil constitute objectives of industry in her effort to cope with the increase of population of earth and disturbance of environment and may ameliorate the effects of global climate change. In the past decades, new biochemical and molecular techniques have been developed in our effort to identify and classify soil bacteria. The goal of measuring the soil microbial diversity is difficult because of the limited knowledge about bacteria species and classification through families and orders. Molecular techniques extend our knowledge about microbial diversity and help the taxonomy of species. Measuring and monitoring soil microbial communities can lead us to better understanding of their composition and function in many ecosystem processes.
- MeSH
- Bacteria izolace a purifikace MeSH
- hybridizace nukleových kyselin MeSH
- kolorimetrie MeSH
- mikroskopie MeSH
- polymerázová řetězová reakce MeSH
- půdní mikrobiologie * MeSH
- sekvenční analýza DNA MeSH
- sekvenční analýza hybridizací s uspořádaným souborem oligonukleotidů MeSH
- Publikační typ
- časopisecké články MeSH
Designing novel isolation methods could enhance the diversification of the available bacterial strains to biotechnology. In this study, the new ex situ and in situ cultivation methods are introduced for the isolation of actinobacteria. In the ex situ experiments, the soil suspension was spread on the isolation media located above some ordinary papers in immediate contact with the slurry of soil substrate and incubated for 16 weeks. The paper was wholly immersed in the cave soil for in situ cultivations, and the containers were buried under layers of soil in Hampoeil cave for 10 weeks. Fruit wrapping kraft coated paper, with 68.8% recovery of isolates, was a better choice in isolation of actinobacteria than other studied filter paper. Based on the molecular identification results, 19% of the isolates obtained from the in situ cultivation method had less than 98.5% similarity to known taxa of actinobacteria and potentially may represent new species. In contrast, in the standard cultivation method, 1.3% of the isolates had less than 98.5% similarity 16Sr RNA gene. This data shows that the introduced cultivation method is a promising technique for isolating less culturable or new actinobacteria.
- MeSH
- Actinobacteria * genetika MeSH
- Bacteria MeSH
- fylogeneze MeSH
- ovoce MeSH
- půdní mikrobiologie MeSH
- RNA ribozomální 16S genetika MeSH
- Publikační typ
- časopisecké články MeSH
