Mammalian adipose tissue derived stem cells (AT-SC) have a tremendous potential in regenerative medicine for tissue engineering and somatic nuclear transfer (SNT). The isolation methods of human and bovine adipose tissue derived stem cells are compared in this paper to determine the feasibility and optimum method of isolation. The optimum isolation method will reduce the processing time, efforts and money as isolation is the first crucial and important step in stem cells research. Human abdominal subcutaneous adipose tissue and bovine abdominal subcutaneous adipose tissue are digested in three collagenase type 1 concentration 0.075%, 0.3% and 0.6% agitated at 1 h and 2 h under 37 °C in 5% CO2 incubator. The cultures are then morphologically characterised. Human adipose tissue stem cells are found to be best isolated using abdominal subcutaneous depot, using 0.075% collagenase type 1 agitated at 1 h under 37 °C in CO2 incubator. While bovine adipose tissue derived stem cells are best isolated using abdominal subcutaneous depot, using 0.6% collagenase type 1 agitated at 2 h under 37 °C in CO2 incubator.
- MeSH
- Cell Differentiation MeSH
- Cell Culture Techniques methods MeSH
- Species Specificity MeSH
- Stem Cells cytology physiology MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Cell Separation methods MeSH
- Cattle MeSH
- Tissue Engineering methods MeSH
- Adipocytes cytology physiology MeSH
- Cell Survival physiology MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- Comparative Study MeSH
V práci sa referuje o ojedinelom prípade izolácie Neisseria meningitidis C:2a:P1.2,P1.5 v auguste1997 od 7-ročnej pacientky z výteru z pošvy pri purulentnom zápale vonkajšieho genitálu po úraze.Ide o prvý popísaný prípad izolácie N. meningitidis z takejto lokalizácie u nás a upozorňuje nanutnosť myslieť na neobvyklý výskyt N. meningitidis, vyšetrovať atypické materiály aj mikroskopic-ky a všetky izolované neisérie vrátane gonokokov identifikovať aj biochemicky.
The authors describe a unique case of isolation of Neisseria meningitidis C:2a:P1.2,P1.5 in August1997 from a 7-year-old female patient from a vaginal smear during purulent inflammation of theexternal genital following injury. This is the first described case of isolation of N. meningitidis fromthis site described in our country. The authors draw attention to the necessity to consider uncom-mon occurrences of N. meningitidis, and to examine atypical materials also under the microscopeand to identify by biochemical methods all isolated Neisseriae incl. gonococci.
- MeSH
- Child MeSH
- Humans MeSH
- Neisseria meningitidis isolation & purification MeSH
- Vagina injuries MeSH
- Vaginitis microbiology MeSH
- Check Tag
- Child MeSH
- Humans MeSH
- Publication type
- Case Reports MeSH
