Matrix Gla protein (MGP), a natural inhibitor of calcification, strongly correlates with the extent of coronary calcification. Vitamin K is the essential cofactor for the activation of MGP. The nonphosphorylated-uncarboxylated isoform of MGP (dp-ucMGP) reflects the status of this vitamin. We investigated whether there is an association between dp-ucMGP and stiffness of elastic and muscular-type large arteries in a random sample from the general population. In a cross-sectional design, we analyzed 1087 subjects from the Czech post-MONICA study. Aortic and femoro-popliteal pulse wave velocities (PWVs) were measured using a Sphygmocor device. Dp-ucMGP concentrations were assessed in freshly frozen samples by enzyme-linked immunosorbent assay methods using the InaKtif MGP iSYS pre-commercial kit developed by IDS and VitaK. Aortic PWV significantly (P<0.0001) increased across the dp-ucMGP quartiles. After adjustment for all potential confounders, aortic PWV independently correlated with dp-ucMGP (with beta coefficient (s.d.) 11.61 (5.38) and P-value=0.031). In a categorized manner, subjects in the top quartile of dp-ucMGP (⩾ 671 pmol l(-1)) had a higher risk of elevated aortic PWV, with corresponding adjusted odds ratio (95% confidence interval) 1.73 (1.17-2.5). In contrast, no relation between dp-ucMGP and femoro-popliteal PWV was found. In conclusion, increased dp-ucMGP, which is a circulating biomarker of vitamin K status and vascular calcification, is independently associated with aortic stiffness, but not with stiffness of distal muscular-type arteries.

• MeSH
• analýza pulzové vlny MeSH
• biologické markery krev   MeSH
• dospělí MeSH
• ELISA MeSH
• extracelulární matrix - proteiny krev   MeSH
• fosforylace MeSH
• lidé středního věku MeSH
• lidé MeSH
• lineární modely MeSH
• logistické modely MeSH
• multivariační analýza MeSH
• nemoci aorty krev   diagnóza   patofyziologie   MeSH
• odds ratio MeSH
• onemocnění periferních arterií krev   diagnóza   patofyziologie   MeSH
• proteiny vázající vápník krev   MeSH
• průřezové studie MeSH
• rizikové faktory MeSH
• senioři MeSH
• tuhost cévní stěny * MeSH
• upregulace MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH

OBJECTIVE: Previous descriptions of potential biomarkers of osteoarthritis (OA) have been limited to Caucasians. In the present study, we examined associations between serum levels of cartilage oligomeric matrix protein (COMP) and ethnicity (African American or Caucasian) and sex in the Johnston County Osteoarthritis Project, a population-based study of OA in rural North Carolina. METHODS: All African Americans and a randomly selected sample of Caucasians who had available sera and either no radiographic evidence of knee or hip OA according to the Kellgren/Lawrence (K/L) system (K/L grade 0) or radiographic evidence of knee OA (K/L grade 2 or higher) were included. Serum COMP levels were quantified by sandwich enzyme-linked immunosorbent assay, using monoclonal antibodies 16-F12 and 17-C10. Linear regression models were used to assess relationships between serum levels of natural log-transformed COMP (ln COMP) and ethnicity and sex, controlling for age, height, body mass index (BMI), radiographic OA, and the presence of other symptomatic joints. Radiographic OA was defined in separate models as the presence, severity, and laterality of radiographic knee OA, the co-occurrence of radiographic knee and hip OA, and the number of knees and hips with radiographic OA. RESULTS: The 769 subjects in the study sample had a mean +/- SD age of 62 +/- 10.3 years. Levels of ln COMP were associated with age, BMI, and all definitions of radiographic OA (P = 0.0001), and varied by ethnicity and sex. In adjusted models, ln COMP was higher in African American women than in Caucasian women (P = 0.003) and higher in Caucasian men than Caucasian women (P = 0.0001). There were no statistically significant differences in serum ln COMP levels between African American men and women. CONCLUSION: Serum COMP levels vary by ethnicity and sex. These factors should be considered in the derivation of standards using this, and possibly other, potential biomarkers of OA.

• MeSH
• artróza kolenních kloubů etnologie   krev   MeSH
• běloši MeSH
• biologické markery krev   MeSH
• černoši nebo Afroameričané MeSH
• etnicita MeSH
• extracelulární matrix - proteiny krev   MeSH
• glykoproteiny krev   MeSH
• kloubní chrupavka MeSH
• kolenní kloub patologie   radiografie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• náhodné rozdělení MeSH
• senioři MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• práce podpořená grantem MeSH
• Research Support, U.S. Gov't, P.H.S. MeSH
• Geografické názvy
• Severní Karolína MeSH

BACKGROUND: Lipoprotein-associated phospholipase A2 (Lp-PLA2) is independently associated with cardiovascular risk, probably via inflammatory activity in sclerotic plaque. We speculated whether Lp-PLA2 has a role in the aetiology of vascular calcifications, estimated from circulating uncarboxylated matrix Gla protein (MGP) species and whether we could find a potential interaction of Lp-PLA2 and MGP in terms of mortality. MATERIALS AND METHODS: We examined 798 patients (mean age 65.1 years) with stable vascular disease and followed them in a prospective study. Both, desphospho-uncarboxylated and total MGP (dp-ucMGP or t-ucMGP) were quantified by pre-commercial ELISA assays, developed by VitaK (Maastricht, The Netherland) RESULTS: Lp-PLA2 activity was independently positively associated with desphospho-uncarboxylated MGP (dp-ucMGP) [β coeff = 0.098, p=0.006]. 1SD of Lp-PLA2 activity was associated with 37% increased risk (p=0.001) of elevated dp-ucMGP (≥977 pmol/L, top quartile). In the Cox proportional hazard model adjusted for conventional risk factors, the patients in the highest quartile of dp-ucMGP or lowest quintile of total-uncarboxylated ucMGP (<2660 nmol/L) had higher risk of all-cause mortality [HRR 2.79 (95% CI 1.97-3.94) and HRR 1.69 (95% CI 1.18-2.42), respectively]. We observed no effect of high Lp-PLA2 activity (≥195 nmol/min/mL) on total mortality. CONCLUSIONS: We assume that Lp-PLA2 is involved in vascular calcification and that dp-ucMGP is a more appropriate biomarker of residual risk than Lp-PLA2 itself.

• MeSH
• 1-alkyl-2-acetylglycerofosfocholinesterasa krev   MeSH
• analýza přežití MeSH
• biologické markery krev   MeSH
• extracelulární matrix - proteiny krev   MeSH
• kardiovaskulární nemoci krev   mortalita   MeSH
• lidé středního věku MeSH
• lidé MeSH
• proporcionální rizikové modely MeSH
• prospektivní studie MeSH
• proteiny vázající vápník krev   MeSH
• rizikové faktory MeSH
• senioři MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Nizozemsko MeSH

Two most influential models of evolutionary game theory are the Hawk-Dove and Prisoner's dilemma models. The Hawk-Dove model explains evolution of aggressiveness, predicting individuals should be aggressive when the cost of fighting is lower than its benefit. As the cost of aggressiveness increases and outweighs benefits, aggressiveness in the population should decrease. Similarly, the Prisoner's dilemma models evolution of cooperation. It predicts that individuals should never cooperate despite cooperation leading to a higher collective fitness than defection. The question is then what are the conditions under which cooperation evolves? These classic matrix games, which are based on pair-wise interactions between two opponents with player payoffs given in matrix form, do not consider the effect that conflict duration has on payoffs. However, interactions between different strategies often take different amounts of time. In this article, we develop a new approach to an old idea that opportunity costs lost while engaged in an interaction affect individual fitness. When applied to the Hawk-Dove and Prisoner's dilemma, our theory that incorporates general interaction times leads to qualitatively different predictions. In particular, not all individuals will behave as Hawks when fighting cost is lower than benefit, and cooperation will evolve in the Prisoner's dilemma.

• MeSH
• agrese MeSH
• biologická evoluce * MeSH
• biologické modely MeSH
• časové faktory MeSH
• interpersonální vztahy * MeSH
• kooperační chování MeSH
• lidé MeSH
• teorie her * MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

OBJECTIVE: To characterize serum cartilage oligomeric matrix protein (COMP) levels by age and gender for a radiographically defined population free of hip and knee osteoarthritis (OA), and to examine the potential utility of COMP as a diagnostic biomarker for knee OA. METHODS: Serum samples and knee and hip radiographs were obtained at a baseline evaluation as part of the Johnston County Osteoarthritis Project, a population-based study of OA in rural North Carolina. A total of 291 Caucasian participants were randomly selected for COMP analysis, 143 patients with radiographic knee OA (Kellgren/Lawrence [K/L] grade > or = 2) and 148 controls with neither hip nor knee OA (K/L grade 0), evenly distributed by age and gender. COMP was quantified by competitive enzyme-linked immunosorbent assay with monoclonal antibody 17-C10. The natural log-transformed COMP data were analyzed using general linear models. RESULTS: Serum COMP levels were significantly elevated (P = 0.0001) in the age > or = 65 group (mean +/- SD 1,302.1 +/- 496.7 ng/ml) versus the age 45-54 and age 55-64 groups (1,058.1 +/- 432.4 and 1,038.6 +/- 313.3, respectively). Serum COMP levels of the OA group were significantly higher than those of the control group (1,208.57 +/- 487.47 ng/ml versus 1,061.83 +/- 370.58 ng/ml; P = 0.0093). Serum COMP levels also increased significantly with knee OA K/L grade (P = 0.0047), knee OA laterality (P = 0.0043), and number of knee and hip joints involved (P = 0.0001). There was no significant difference in serum COMP levels by gender or obesity. CONCLUSION: We demonstrate that in a population-based sample, serum COMP levels can distinguish an OA-affected subgroup from an unaffected subgroup and can reflect disease severity and multiple joint involvement in OA.

• MeSH
• artróza kolenních kloubů * krev   radiografie   MeSH
• biologické markery krev   MeSH
• extracelulární matrix - proteiny * krev   MeSH
• glykoproteiny * krev   MeSH
• kolenní kloub radiografie   MeSH
• kyčelní kloub radiografie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• progrese nemoci MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• práce podpořená grantem MeSH
• Research Support, U.S. Gov't, P.H.S. MeSH

Matrix metalloproteinases (MMPs) are a family of zinc-dependent metalloendopeptidases that degrades extracellular matrix (ECM) components. MMPs are associated with venous wall remodelling, proliferation, migration, phenotypic and functional transformation of vascular smooth muscle cells and ECM organization under the physiological and pathophysiological conditions. We investigated possible association of genetic promoter polymorphisms of MMP2 (rs243866), MMP8 (rs11225395), MMP9 (rs3918242) and TIMP2 (rs8179090) to varicose veins development in the Slovak population. Genomic DNA from 276 Slovak individuals (138 cases, 138 controls) was genotyped for selected SNPs (rs243866, rs11225395, rs3918242 and rs8179090) using the PCR-RFLP analysis. The data were analysed by chi-squared (chi2) test, logistic regression, and Mann-Whitney test. The risk of varicose veins development was evaluated in dominant, codominant and recessive genetic models. The statistical evaluation of selected polymorphisms in patients in all three genetic models has not shown a significant risk of varicose veins development. Our study has not shown the association between selected polymorphisms and increased risk of varicose veins development in Slovak population. More evidence with broaden sample size is needed.

• MeSH
• dospělí MeSH
• genetická predispozice k nemoci MeSH
• genotyp MeSH
• jednonukleotidový polymorfismus MeSH
• lidé středního věku MeSH
• lidé MeSH
• matrixová metaloproteinasa 2 genetika   MeSH
• matrixová metaloproteinasa 8 genetika   MeSH
• matrixová metaloproteinasa 9 genetika   MeSH
• matrixové metaloproteinasy genetika   MeSH
• mladiství MeSH
• mladý dospělý MeSH
• pilotní projekty MeSH
• promotorové oblasti (genetika) MeSH
• senioři MeSH
• studie případů a kontrol MeSH
• tkáňový inhibitor metaloproteinasy 2 genetika   MeSH
• varixy epidemiologie   genetika   patologie   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Slovenská republika MeSH

Aberrant antioxidant activity and excessive deposition of extracellular matrix (ECM) are hallmarks of interstitial lung diseases (ILD). It is known that oxidative stress alters the ECM, but extracellular antioxidant defence mechanisms in ILD are incompletely understood. Here, we extracted abundance and detergent solubility of extracellular antioxidant enzymes from a proteomic dataset of bleomycin-induced lung fibrosis in mice and assessed regulation and distribution of glutathione peroxidase 3 (GPX3) in murine and human lung fibrosis. Superoxide dismutase 3 (Sod3), Gpx3, and Gpx activity were increased in mouse BALF during bleomycin-induced lung fibrosis. In lung tissue homogenates, Gpx3, but not Sod3, was upregulated and detergent solubility profiling indicated that Gpx3 associated with ECM proteins. Immunofluorescence analysis showed that Gpx3 was expressed by bronchial epithelial cells and interstitial fibroblasts and localized to the basement membrane and interstitial ECM in lung tissue. As to human ILD samples, BALF of some patients contained high levels of GPX3, and GPX3 was upregulated in lung homogenates from IPF patients. GPX3 expression in primary human bronchial epithelial cells and lung fibroblasts was downregulated by TNF-α, but more variably regulated by TGF-β1 and menadione. In conclusion, the antioxidant enzyme GPX3 localizes to lung ECM and is variably upregulated in ILD.

• MeSH
• antioxidancia metabolismus   MeSH
• bleomycin MeSH
• bronchoalveolární lavážní tekutina MeSH
• bronchy patologie   MeSH
• demografie MeSH
• down regulace účinky léků   MeSH
• epitelové buňky enzymologie   MeSH
• extracelulární matrix enzymologie   MeSH
• fibroblasty účinky léků   metabolismus   MeSH
• glutathionperoxidasa metabolismus   MeSH
• intersticiální plicní nemoci enzymologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• modely nemocí na zvířatech MeSH
• myši inbrední C57BL MeSH
• oxidační stres účinky léků   MeSH
• plicní fibróza enzymologie   MeSH
• senioři MeSH
• TNF-alfa metabolismus   MeSH
• transformující růstový faktor beta1 metabolismus   MeSH
• upregulace MeSH
• vitamin K 3 farmakologie   MeSH
• zvířata MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

In the adult brain, the extracellular matrix (ECM) influences recovery after injury, susceptibility to mental disorders, and is in general a strong regulator of neuronal plasticity. The proteoglycan aggrecan is a core component of the condensed ECM structures termed perineuronal nets (PNNs), and the specific role of PNNs on neural plasticity remains elusive. Here, we genetically targeted the Acan gene encoding for aggrecan using a novel animal model. This allowed for conditional and targeted loss of aggrecan in vivo, which ablated the PNN structure and caused a shift in the population of parvalbumin-expressing inhibitory interneurons toward a high plasticity state. Selective deletion of the Acan gene in the visual cortex of male adult mice reinstated juvenile ocular dominance plasticity, which was mechanistically identical to critical period plasticity. Brain-wide targeting improved object recognition memory.SIGNIFICANCE STATEMENT The study provides the first direct evidence of aggrecan as the main functional constituent and orchestrator of perineuronal nets (PNNs), and that loss of PNNs by aggrecan removal induces a permanent state of critical period-like plasticity. Loss of aggrecan ablates the PNN structure, resulting in invoked juvenile plasticity in the visual cortex and enhanced object recognition memory.

• MeSH
• agrekany analýza   nedostatek   genetika   MeSH
• buněčné linie MeSH
• extracelulární matrix chemie   genetika   metabolismus   MeSH
• myši inbrední C57BL MeSH
• myši knockoutované MeSH
• myši transgenní MeSH
• myši MeSH
• nervová síť chemie   metabolismus   MeSH
• neuroplasticita fyziologie   MeSH
• světelná stimulace metody   MeSH
• zrakové korové centrum chemie   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Dynamiku růstu tělesné výšky i všech dalších somatických parametrů dítěte vystihují rychlostní růstové křivky, které znázorňují rychlost růstu daného znaku v jednotlivých věkových obdobích, vyjádřenou v podobě přírůstku za kalendářní rok. Rychlostní křivky jsou konstruovány na základě dat longitudinálních růstových studií, poskytující údaje o individuálním průběhu růstu s ohledem na jeho interindividuální variabilitu v příslušné populaci. Průměrné rychlostní křivky daného znaku pak slouží jako normativy pro posouzení jeho růstové rychlosti, která je u základních tělesných parametrů, jako např. tělesná výška, limitujícím kritériem v diagnostice růstových poruch. Předpokladem longitudinální studie je dlouhodobé sledování stejných jedinců a jejich opakovaná vyšetření v přesně definovaných intervalech. Z toho plyne řada komplikací, mezi něž patří vysoká organizační náročnost projektu, většinou podstatně omezená četnost souboru a postupná ztráta reprezentativnosti souboru, kdy se často řada jedinců k opakovaným vyšetřením nedostaví. Problémem je i ztráta aktuálnosti dat. Řešením uvedených nedostatků longitudinální studie je volba semilongitudinálního sledování, koncipovaného tak, že se jedná o krátká longitudinální sledování různých věkových skupin jedinců, která probíhají ve stejném čase. Při ukončení studie na sebe jednotlivé skupiny v přesně definovaném věku navazují. Výhodou správně koncipovaného navazujícího semilongitudinálního sledování je možnost shromáždění reprezentativního souboru dat v krátkém časovém intervalu. Takto získaná data lze díky vhodně zvoleným matematickým modelům využít ke konstrukci rychlostních křivek tělesných parametrů. V letech 1997 až 2000 bylo celkem antropometricky vyšetřeno 1925 dětí (990 chlapců, 935 dívek) ve věku 6-15 let. Antropometrická měření probíhala v přesně vymezených půlročních intervalech tak, aby výsledná matice dat splňovala podmínky napojovaného longitudinálního sledování. Sledováno bylo celkem 29 somatických znaků, prezentovány jsou charakteristiky růstové dynamiky tělesné výšky, výšky vsedě, sub-ischiální délky a délky horní končetiny. Ke konstrukci průměrných rychlostních křivek byl zvolen model lineární regrese se smíšenými efekty. Průměrná distanční křivka parametru byla konstruována jako polynom 7. stupně. Průměrná rychlostní křivka pak byla získána první derivací rovnice křivky distanční. Použitý matematický model citlivě zachytil obě nejvýraznější růstové akcelerace v dětství - mid-growth spurt (MGS) a pubertální spurt (PS). Byly vypočteny body lokálních maxim a minim jednotlivých spurtů a body inflexe. Na základě těchto charakteristik byla provedena podrobná analýza růstové dynamiky jednotlivých somatických znaků a stanovena průměrná doba délky jednotlivých etap obou růstových spurtů. Při orientačním porovnání věkových charakteristik rychlostních křivek semilongitudinálního souboru s průměrnými daty Curyšské longitudinální studie byl potvrzen nižší věk nástupu i vrcholu PS u dnešních dětí. průměrnými daty Curyšské longitudinální studie byl potvrzen nižší věk nástupu i vrcholu PS u dnešních dětí.

The dynamism of growth of stature and all other somatic parameters of the child give a true picture of the growth curves, which indicate the velocity of growth of a given parameter in individual age periods, expressed in the form of increments over a calendar year. The velocity curves are constructed on the basis of data of longitudinal growth studies, providing g the data on individual course of growth with respect to individual variability in a given population. The mean growth curves of the given characteristic the serve as standards for the evaluation of his/her growth velocity, which is - in basic physical parameters, such as stature - a limiting criterion in the diagnostics of growth disorders. The prerequisite of a longitudinal study is a long-term observation of the same individuals and their repeated examination in precisely defined intervals. Various complications follow, including high organisational requirements of the project, mostly very limited size of the cohort and gradual loss of representative qualities of the group, where numerous individual fail to show up in repeated examinations. The loss of up-to-dateness is a problem. The given shortcomings of a longitudinal study may be solved by selecting a semi-longitudinal study, organised in such a way that it is a short longitudinal observation of different age groups of individual which occurs at the same time. When the study is finished, the individual groups are connected at a precisely defined age. The advantage of a correctly designed connected semilongitudinal observation is the possibility to collect a representative series of data in a short period of time. Such data can be used due to suitable selected mathematical models for the construction of velocity curves of physical parameters. In the years 1997 to 2000, a total of 1925 children (990 boys and 935 girls) were examined by anthropometric methods at the age of 6-15 years. Anthropometric measurements were precisely in half-year intervals in such a way that the resulting matrix of data fulfilled the conditions of connected longitudinal observation. The authors followed 29 somatic characteristics on the whole, characteristics of the broth dynamics of stature, sitting height, subischial length and the length of upper extremity. For the construction of the mean growth curves the authors selected a model of linear regression with mixed effects. The mean distance curve of the parameters was constructed as a polynomial of 7th degree. The mean velocity curve was then reached by means of derivative equation of distance curve. The used mathematical model was sensitive enough to detect both most prominent growth accelerations in the childhood - mid-growth spurt (MGS) a pubertal spurt (PS). Local maxima and minima of individual spurts and inflexion points were calculated. Based on these characteristics the authors performed a detailed analysis of the growth dynamism of individual growth attributes and determined the average period of time of individual stages of both growth spurts. In an orientational comparison of the age characteristics of velocity curves of the semi-longitudinal cohort with the mean values of the Zurich longitudinal study a younger age of the onset and peak of PS in the present children was confirmed.

• MeSH
• antropometrie MeSH
• financování vládou MeSH
• kontrola růstu populace statistika a číselné údaje   trendy   MeSH
• lidé MeSH
• muži MeSH
• poruchy růstu epidemiologie   etiologie   MeSH
• růst a vývoj fyziologie   MeSH
• ženy MeSH
• Check Tag
• lidé MeSH

We assessed association between novel biomarkers of cardiovascular disease and conventional factors in 40 years old subjects (208 men and 266 women) from the general population of Slovakia. FERHDL (cholesterol esterification rate in HDL plasma), AIP – Atherogenic Index of Plasma [Log(TG/HDL-C)] as markers of lipoprotein particle size, and CILP2, FTO and MLXIPL polymorphisms, were examined in relation to biomarkers and conventional risk factors. Univariate analyses confirmed correlation between AIP, FERHDL and the most of measured parameters. Relations between AIP and CILP2, FTO and MLXIPL were not significant. However, CILP2 was significantly related to FERHDL in both genders. In multivariate analysis BMI was the strongest correlate of AIP levels. In a model where AIP was omitted, TG levels explained 43 % of the FERHDL variability in men, while in women HDL-C was the major determinant (42 %). In conclusions, FERHDL and AIP related to the known markers of cardiovascular risk provide means to express their subtle interactions by one number. Our novel finding of association between CILP2 polymorphism and FERHDL supports its role in lipid metabolism.

• MeSH
• dospělí MeSH
• esterifikace MeSH
• extracelulární matrix - proteiny genetika   MeSH
• genetická predispozice k nemoci epidemiologie   genetika   MeSH
• HDL-cholesterol krev   MeSH
• hodnocení rizik MeSH
• jednonukleotidový polymorfismus genetika   MeSH
• lidé MeSH
• nemoci koronárních tepen epidemiologie   genetika   krev   MeSH
• prevalence MeSH
• pyrofosfatasy genetika   MeSH
• rizikové faktory MeSH
• statistika jako téma MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• práce podpořená grantem MeSH
• Geografické názvy
• Slovenská republika MeSH