scanning probe microscopy
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elektronický časopis
- Konspekt
- Biologické vědy
- NLK Obory
- biologie
- chemie, klinická chemie
- NLK Publikační typ
- elektronické časopisy
AFM (atomic force microscopy) nanolithography can be used for preparation of nanostructures in various fields such as nanodevices, nanoantenas and biosensors. Several methods of AFM nanolithography (local anodic oxidation, electron resist exposure, dip pen nanolithography and nanoscratching), their advantages and essential properties are described.
We report a new configuration for enhancing the performance of scanning electrochemical microscopy (SECM) via heating of the substrate electrode. A flattened Pt microwire was employed as the substrate electrode. The substrate was heated by an alternating current (AC), resulting in an increased mass transfer between the wire surface and the bulk solution. The electrochemical response of the Pt wire during heating was investigated by means of cyclic voltammetry (CV). The open circuit potential (OCP) of the wire was recorded over time, while varied heating currents were applied to investigate the time needed for establishing steady-state conditions. Diffusion layer studies were carried out by performing probe approach curves (PACs) for various measuring modes of SECM. Finally, imaging studies of a heated substrate electrode surface, applying feedback, substrate generation/tip collection (SG/TC), and the competition mode of SECM, were performed and compared with room temperature results.
The present study is bringing a comparison of surface morphology for various types of contact lenses. A novel method--scanning electron microscopy under aqueous conditions (cryo-SEM)--was tested for visualization of lenses at magnifications up to 2000x. For imaging lens surface on nanometre scale, we employed atomic force microscopy (AFM) in aqueous media. Various materials of lenses, based on silicone hydrogels or conventional hydrogels, were investigated. Total, 10 types of contact lenses from five manufacturers were selected and probed. We found that different methods of lens manufacture (lathe-cutting, cast-moulding, and spin casting) led to different values of surface roughness. In the swollen state, roughness values of lens surfaces lie between 4 and 140 nm. Lenses manufactured by lathe-cutting exhibit notable higher values, so that they could be easily distinguished from others. In cast-moulded lenses, the surface roughness decreased with increasing water content. Moreover, additional treatments of lenses introduced unique structural motifs onto surface. For instance, porous structure was found on lens surface finalized with plasma oxidation.
- MeSH
- biomedicínské technologie MeSH
- financování organizované MeSH
- hydrogely MeSH
- kontaktní čočky MeSH
- lidé MeSH
- mikroskopie atomárních sil metody MeSH
- mikroskopie elektronová rastrovací metody MeSH
- nanotechnologie MeSH
- povrchové vlastnosti MeSH
- roztoky MeSH
- silikony MeSH
- zmrazování MeSH
- Check Tag
- lidé MeSH
Mezi hlavni přednosti konfokální mikroskopie ve srovnaní s klasickou optickou mikroskopii patři možnost zaznamenávat série digitalizovaných obrazů relativně tenkých optických řezů vedených i tlustším vzorkem. V článkuje ukázáno využití těchto sérií obrazů, jak při vizualizaci, tak i při kvantifikaci trojrozměrných objektů: Jsou popsány různé typy rekonstrukcí objektů, které lze proostřit konfokálním mikroskopem, i měření jejich geometrických charakteristik s využitím stereologických metod. Podrobněji je přiblížena stereologická metoda užívající virtuální fakirské sondy pro odhad objemu i povrchu studovaného objektu.
The possibility to record series of digitized images of relatively thin optical sections within a thick speciment is one of the main advantages ofconfocal mircoscopy if compared with the conventional optical microscopy. It is shown how such series can be exploited in visualization and quantification of three-dimensional objects: different types of reconstructions of objects that can be focussed through by a confocal microscope as well as the estimation of their geometrical characteristics by stereological methods are described. The stereological method using virtual fakir probes for volume and surface area estimation is presented in detail.
- MeSH
- konfokální mikroskopie MeSH
- lidé MeSH
- software MeSH
- výpočetní biologie MeSH
- Check Tag
- lidé MeSH
... www.laryngoscope.com -- FACIAL PLASTICS/RECONSTRUCTIVE SURGERY -- THE ROLE OF THE IMPLANTABLE DOPPLER PROBE ...
The laryngoscope, ISSN 0023-852X Volume 124, supplementum 1 , March 2014
12 stran : ilustrace, tabulky ; 28 cm
- MeSH
- nemoci cév MeSH
- peroperační monitorování MeSH
- pooperační komplikace prevence a kontrola MeSH
- pooperační péče MeSH
- protézy a implantáty MeSH
- skenovací elektrochemická mikroskopie MeSH
- ultrasonografie dopplerovská MeSH
- volné tkáňové laloky MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- otorinolaryngologie
- chirurgie
- NLK Publikační typ
- studie
OBJECTIVE: To determine the three-dimensional (3D) position of target sequences and chromosomal volumes in interphase human nuclei by confocal laser scanning microscopy (CLSM) by using the heterochromatin part of the long arm of human chromosome Y (HPLAHC) as a target for specific DYZ1 probes, then by D10Z1 probes specific to the centromere of chromosome 10. STUDY DESIGN: Fluorescence in situ hybridization information inside chromosomal preparations was obtained with FITC-labelled probes and propidium iodide (PI) as a DNA-specific stain. To have a control in the experiment, HPLAHC Y was taken as a model of a domain and the centromere of chromosome 10 as a model of a single centromere spot. To have access to their 3D visualization, we selected FITC and PI patterns of fluorescence when optical slices were obtained and used a 3D reconstruction software. RESULTS: Labelling of the target by the probes was characteristic of Y heterochromatin and chromosome 10 centromere localizations and allowed observation of their domain in the x, y and z directions. CONCLUSION: This work was performed on two sets of 30 stained interphase nuclei. Deformations were confirmed by fluorescent spherical beads mounted in the same medium and scanned in the same conditions.
- MeSH
- buněčné jádro MeSH
- centromera MeSH
- chromozom Y * MeSH
- DNA sondy MeSH
- heterochromatin MeSH
- hybridizace in situ fluorescenční * metody MeSH
- interfáze MeSH
- konfokální mikroskopie MeSH
- kultivované buňky MeSH
- lidé MeSH
- lidské chromozomy, pár 10 * MeSH
- lymfocyty cytologie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
