2nd ed. xviii, 826 s. : il. ; 25 cm
- MeSH
- Diagnostic Imaging MeSH
- Image Processing, Computer-Assisted MeSH
- Publication type
- Handbook MeSH
- Conspectus
- Speciální počítačové metody. Počítačová grafika
- NML Fields
- lékařská informatika
Přes pokrok, kterého bylo dosaženo v posledních desetiletích, zůstává postintubační subglotická stenóza u dětí závažným léčebným problémem. Jednu z možností chirurgické léčby představují dilatační laryngotracheoplastiky. Autoři ve sdělení uvádějí zkušenosti s řešením postin- tubační stenózy u osmiletého chlapce. Po neúspěchu konzervativní léčby provedli jednofázovou přední laryngotracheoplastiku s dilatačním štěpem autologní chrupavky ze žebra. Pooperační průběh byl komplikován granulačním procesem, k jehož zvládnutí byla nutná retracheotomie, která prodloužila pacientovu závislost na tracheostomické kanyle o tři měsíce. Rok a půl po operaci je hoch zcela bez dechových potíží.
Despite advances achieved in the last decades postintubation subglottic stenosis remains a serious therapeutic problem in children. One of the possible surgical approaches is dilatation laryngotracheoplasty. The authors present in their paper their experience with postin- tubations stenosis in a 8-year-old boy. After failure of conservative treatment they performed a one-stage anterior laryngotracheoplasty with a dilatation graft of autologous cartilage from a rib. The postoperative course was complicated by a granulation process on account of which retrache- otomy had to be performed which protracted the patient’s dependence on a tracheostomic cannula by three monhts. One and a half year after operation the boy has no respiratory complaints.
- MeSH
- Dilatation methods MeSH
- Child MeSH
- Intubation, Intratracheal adverse effects MeSH
- Humans MeSH
- Tracheal Stenosis MeSH
- Tracheotomy MeSH
- Check Tag
- Child MeSH
- Humans MeSH
- Male MeSH
- Publication type
- Case Reports MeSH
Tento článek popisuje vybrané problémy v oblasti elektroencefalografi e, současné přístupy k jejich řešení a trendy vývoje a výzkumu. Stručně jsou popsány metody počítačové klasifi kace EEG záznamů a eliminace artefaktů v nich. Dále následuje několik pokročilejších metod zpracování a praktického využití EEG, mezi něž patří teorie chaosu, analýza nezávislých komponent, skryté Markovovy modely, rozhraní mozek-počítač a EEG biofeedback. Na závěr je krátce pojednáno o přenosných EEG systémech malých rozměrů.
This paper describes selected problems in the area of electroencephalography, current approaches to their solution and trends in research and development. Methods of computer-assisted classifi cation and artifacts removing from EEG signals are briefl y mentioned. Th is paper also introduce several advanced methods of computer processing and practical use of EEG, namely Chaos theory, Independent Component Analysis, Hidden Markov models, Brain computer interface and EEG biofeedback. Small portable EEG systems are introduced at the end of paper.
- MeSH
- Monitoring, Ambulatory methods instrumentation utilization MeSH
- Principal Component Analysis MeSH
- Biomedical Technology instrumentation trends MeSH
- Time Factors MeSH
- Electroencephalography instrumentation trends utilization MeSH
- Financing, Organized MeSH
- Fractals MeSH
- Humans MeSH
- Nonlinear Dynamics MeSH
- Signal Processing, Computer-Assisted instrumentation MeSH
- Telemetry methods instrumentation utilization MeSH
- User-Computer Interface MeSH
- Feedback MeSH
- Check Tag
- Humans MeSH
V procese výskumu a vývoje liečiva má svoje nezastupiteľné miesto aj sledovanie stability liečiva, resp. lieku. V tomto príspevku je to potenciálne liečivo H+B, chemicky N-[2-(2-heptyloxyfenylkarbamoyloxy)-etyl]-N-benzylpipendiniumchlorid, s výrazným antiarytmickým a tiež lokálne anestetickým účinkom. Sledovala sa stabilita potenciálneho liečiva H+B v roztoku bez a v prítomnosti polyolov za účelom formulácie injekčného roztoku pre intramuskulámu aplikáciu.
In the process of drug research and development an irreplaceable stage is the examination of stability of the drug or the pharmaceutical preparation. The present paper examines the potential drug H+B, chemically N-[2-(2-heptyloxyphenylcarbamoyloxy)-ethyl]-N-benzylpiperidinium chloride, an agent with a marked anti-arrhytmic and local anaesthetic effect. Stability tests aim to examine the effects of polyols on the stability of the potential drug H+B from the standpoint of the formulation of injection solution for intramuscular administration.
Von Willebrand Factor (vWF), a 300-kDa plasma protein key to homeostasis, is cleaved at a single site by multi-domain metallopeptidase ADAMTS-13. vWF is the only known substrate of this peptidase, which circulates in a latent form and becomes allosterically activated by substrate binding. Herein, we characterised the complex formed by a competent peptidase construct (AD13-MDTCS) comprising metallopeptidase (M), disintegrin-like (D), thrombospondin (T), cysteine-rich (C), and spacer (S) domains, with a 73-residue functionally relevant vWF-peptide, using nine complementary techniques. Pull-down assays, gel electrophoresis, and surface plasmon resonance revealed tight binding with sub-micromolar affinity. Cross-linking mass spectrometry with four reagents showed that, within the peptidase, domain D approaches M, C, and S. S is positioned close to M and C, and the peptide contacts all domains. Hydrogen/deuterium exchange mass spectrometry revealed strong and weak protection for C/D and M/S, respectively. Structural analysis by multi-angle laser light scattering and small-angle X-ray scattering in solution revealed that the enzyme adopted highly flexible unbound, latent structures and peptide-bound, active structures that differed from the AD13-MDTCS crystal structure. Moreover, the peptide behaved like a self-avoiding random chain. We integrated the results with computational approaches, derived an ensemble of structures that collectively satisfied all experimental restraints, and discussed the functional implications. The interaction conforms to a 'fuzzy complex' that follows a 'dynamic zipper' mechanism involving numerous reversible, weak but additive interactions that result in strong binding and cleavage. Our findings contribute to illuminating the biochemistry of the vWF:ADAMTS-13 axis.
- MeSH
- Kinetics MeSH
- Humans MeSH
- Models, Molecular MeSH
- Peptides chemistry MeSH
- Protein Processing, Post-Translational * MeSH
- ADAMTS13 Protein metabolism MeSH
- Cross-Linking Reagents chemistry MeSH
- Solutions MeSH
- Protein Binding MeSH
- von Willebrand Factor chemistry isolation & purification metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
This paper brings a new rigorous and complete statistical approach to the data processing of the mobility curves of univalent weak bases. This approach is based on application of the least square method to the equation of the related mobility curve. Thus, an equation for the best fit is derived and its mathematical solution is found. The solution brings best estimates of the mobility curve parameters, i.e., dissociation constant K and ionic mobility of the protonated base U. Further, explicit formulas have been derived for the calculation of related statistical parameters, i.e., SDs of effective mobility s(u), of the dissociation constant s(K), and of ionic mobility of protonated base s(U). The mathematical functions used in the above approach do not impose any limitations on the data used, i.e., the mobility and pH values used may be real numbers (positive, negative, zero).
