Transmission of canine leishmaniosis (CanL) is described in three consecutive generations of female Boxers living in a non-endemic environment in the Czech Republic. Infection of the first generation female likely occurred during a breeding visit to Italy and the dog died with typical clinical signs of the disease but without definitive laboratory diagnosis. The second and third generation offsprings never left the Czech Republic, suffered from clinical CanL confirmed by polymerase chain reaction and serology, and were apparently infected by transplacental transmission. Persistence of CanL in the Czech Republic over 7 years with a suspected origin in an endemic region and progression of infection through subsequent generations in a non-endemic country exemplifies that this disease may establish itself also in areas where no obvious vectors are present.
- MeSH
- Leishmania genetika izolace a purifikace MeSH
- leishmanióza epidemiologie parazitologie přenos veterinární MeSH
- nemoci psů epidemiologie parazitologie přenos MeSH
- polymerázová řetězová reakce veterinární MeSH
- psi MeSH
- vertikální přenos infekce veterinární MeSH
- zvířata MeSH
- Check Tag
- psi MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika epidemiologie MeSH
Trichomonads are a group of anaerobic flagellates. Two species of intestinal trichomonads have been described in cats - pathogenic Tritrichomonas foetus and probably harmless Pentatrichomonas hominis. InPouch™ TF-Feline medium (Bio-Med Diagnostics, White City, Oregon, USA) is considered the gold standard for diagnosis of T. foetus infection in cats. It is commercially available, relatively cheap and easy-to-use. The medium is intended to be highly specific to T. foetus as morphologically similar Pentatrichomonas hominis and Giardia sp. do not survive here longer than 24h. In this study we successfully cultivated P. hominis in InPouch™ TF-Feline medium for 3 days after inoculation with cat faeces. The identity of the organism was assessed by sequencing of SSU rDNA and ITS region. Possible coinfection with T. foetus was ruled out using Tritrichomonas-specific PCR. Our results suggest possible misdiagnosis of tritrichomonosis in cats using InPouch™ TF-Feline medium. PCR-based verification of culture-positive samples prior the potentially neurotoxic ronidazole treatment is recommended.
- MeSH
- feces parazitologie MeSH
- kočky MeSH
- kultivační média MeSH
- nemoci koček diagnóza parazitologie MeSH
- protozoální infekce zvířat diagnóza parazitologie MeSH
- Tritrichomonas foetus izolace a purifikace MeSH
- zvířata MeSH
- Check Tag
- kočky MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
91 s. : il., tab. ; 21 cm
- MeSH
- biomedicínský výzkum MeSH
- echinokokóza diagnóza prevence a kontrola terapie MeSH
- rizikové faktory MeSH
- trichinelóza diagnóza prevence a kontrola terapie MeSH
- zdroje nemoci MeSH
- zoonózy epidemiologie MeSH
- Publikační typ
- příručky MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- parazitologie
- infekční lékařství
OBJECTIVE: Angiotensin II and aldosterone, generated by the angiotensin-converting enzyme (ACE) and aldosterone synthase (CYP11B2), respectively, not only regulate sodium and water homeostasis, but also influence vascular remodeling in response to high blood pressure. In the European Project on Genes in Hypertension (EPOGH), we therefore investigated whether the ACE I/D and CYP11B2 C-344T polymorphisms influence early arterial wave reflections, a measure of vascular stiffness. METHODS: We measured the peripheral and central augmentation index of systolic blood pressure by applanation tonometry at the level of the radial artery in 622 subjects (160 families and 64 unrelated individuals) randomly recruited from three European populations, whose average urinary sodium excretion ranged from 196 to 245 mmol/day. In multivariate analyses, with sodium excretion analyzed as a continuous variable, we explored the phenotype-genotype associations by means of generalized estimating equations and the quantitative transmission disequilibrium test. RESULTS: The peripheral and central augmentation indexes were significantly higher in CYP11B2 -344C allele carriers than in -344T homozygotes. In offspring, early wave reflections increased with the transmission of the -344C allele. This effect of the CYP11B2 polymorphism occurred in subjects with a higher than median urinary sodium excretion (210 mmol/day). The ACE I/D polymorphism did not influence augmentation of systolic blood pressure. CONCLUSIONS: The CYP11B2 C-344T polymorphism affects arterial stiffness. However, sodium intake seems to modulate this genetic effect.
- MeSH
- alely MeSH
- angiotensin konvertující enzym genetika MeSH
- arterie patofyziologie MeSH
- cytochrom P450 CYP11B2 genetika MeSH
- cytosin MeSH
- delece genu MeSH
- dospělí MeSH
- heterozygot MeSH
- hypertenze genetika patofyziologie MeSH
- krevní tlak genetika MeSH
- lidé středního věku MeSH
- lidé MeSH
- natriuréza MeSH
- polymorfismus genetický MeSH
- thymin MeSH
- transpozibilní elementy DNA MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
Přeruš. str. : il., tab. ; 32 cm
Vztahy vlastností cévní stěny, kardiovaskulární rizika, hladiny homocysteinu a antropometrických parametrů u probandů nad 60 let s hypertenzí.; The relationships of properties of large arteries, cardiovascular risk profile, homocysteine level and antrhropometric parameters in subjects over 60 years with hypertension.
- MeSH
- diagnostické techniky kardiovaskulární využití MeSH
- hemodynamika MeSH
- hypertenze MeSH
- měření krevního tlaku MeSH
- prognóza MeSH
- rizikové faktory MeSH
- senioři MeSH
- výběr pacientů MeSH
- Check Tag
- senioři MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- kardiologie
- angiologie
- NLK Publikační typ
- závěrečné zprávy o řešení grantu IGA MZ ČR
- MeSH
- demence MeSH
- hydrochlorthiazid aplikace a dávkování terapeutické užití MeSH
- hypertenze etiologie farmakoterapie MeSH
- krevní tlak statistika a číselné údaje MeSH
- lidé středního věku MeSH
- lidé MeSH
- nitrendipin aplikace a dávkování terapeutické užití MeSH
- riziko MeSH
- senioři MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- senioři MeSH
