BACKGROUND: Adipose tissue is significantly involved in inflammatory bowel disease (IBD). Vitamin D can affect both adipogenesis and inflammation. The aim of this study was to compare the production of selected adipokines, potentially involved in the pathogenesis of IBD - adiponectin, resistin, retinol binding protein 4 (RBP-4), adipocyte fatty acid binding protein and nesfatin-1 in children with IBD according to the presence of 25-hydroxyvitamin D (25(OH)D) deficiency. METHODS: The study was conducted as a case-control study in pediatric patients with IBD and healthy children of the same sex and age. In addition to adipokines and 25(OH)D, anthropometric parameters, markers of inflammation and disease activity were assessed in all participants. RESULTS: Children with IBD had significantly higher resistin levels regardless of 25(OH)D levels. IBD patients with 25(OH)D deficiency only had significantly lower RBP-4 compared to healthy controls and also compared to IBD patients without 25(OH)D deficiency. No other significant differences in adipokines were found in children with IBD with or without 25(OH)D deficiency. 25(OH)D levels in IBD patients corelated with RBP-4 only, and did not correlate with other adipokines. CONCLUSIONS: Whether the lower RBP-4 levels in the 25(OH)D-deficient group of IBD patients directly reflect vitamin D deficiency remains uncertain. The production of other adipokines does not appear to be directly related to vitamin D deficiency.
- MeSH
- adipokiny * krev MeSH
- adiponektin krev nedostatek MeSH
- biologické markery krev MeSH
- dítě MeSH
- DNA vazebné proteiny krev MeSH
- idiopatické střevní záněty krev komplikace MeSH
- lidé MeSH
- mladiství MeSH
- nedostatek vitaminu D * komplikace krev MeSH
- nukleobindiny krev MeSH
- plazmatické proteiny vázající retinol metabolismus analýza MeSH
- proteiny vázající mastné kyseliny krev MeSH
- proteiny vázající vápník krev MeSH
- resistin krev MeSH
- studie případů a kontrol MeSH
- vitamin D * krev analogy a deriváty MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
BACKGROUND AND AIMS: Matrix Gla protein (MGP) is a natural inhibitor of vascular calcification critically dependent on circulating vitamin K status. Growth differentiation factor 15 (GDF-15) is a regulatory cytokine mainly of the inflammatory and angiogenesis pathways, but potentially also involved in bone mineralization. We sought to determine whether these two circulating biomarkers jointly influenced morbidity and mortality risk in patients with chronic coronary heart disease (CHD). METHODS AND RESULTS: 894 patients ≥6 months after myocardial infarction and/or coronary revascularization at baseline were followed in a prospective study. All-cause and cardiovascular mortality, non-fatal cardiovascular events (myocardial infarction, stroke, any revascularization), and hospitalization for heart failure (HF) were followed as outcomes. Desphospho-uncarboxylated MGP (dp-ucMGP) was used as a biomarker of vitamin K status. Both, increased concentrations of dp-ucMGP (≥884 pmol/L) and GDF-15 (≥1339 pg/mL) were identified as independent predictors of 5-year all-cause or cardiovascular mortality. However, their coincidence further increased mortality risk. The highest risk was observed in patients with high dp-ucMGP plus high GDF-15, not only when compared with those with "normal" concentrations of both biomarkers [HR 5.51 (95% CI 2.91-10.44), p < 0.0001 and 6.79 (95% CI 3.06-15.08), p < 0.0001 for all-cause and cardiovascular mortality, respectively], but even when compared with patients with only one factor increased. This pattern was less convincing with non-fatal cardiovascular events or hospitalization for HF. CONCLUSIONS: The individual coincidence of low vitamin K status (high dp-ucMGP) and high GDF-15 expression predicts poor survival of stable CHD patients.
- MeSH
- biologické markery krev MeSH
- časové faktory MeSH
- chronická nemoc MeSH
- extracelulární matrix - proteiny krev MeSH
- hodnocení rizik MeSH
- incidence MeSH
- koronární nemoc krev diagnóza mortalita terapie MeSH
- lidé středního věku MeSH
- lidé MeSH
- nedostatek vitaminu D krev diagnóza mortalita MeSH
- prognóza MeSH
- prospektivní studie MeSH
- proteiny vázající vápník krev MeSH
- průřezové studie MeSH
- rizikové faktory MeSH
- růstový diferenciační faktor 15 krev MeSH
- senioři MeSH
- upregulace MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
BACKGROUND: Matrix Gla protein (MGP) is a natural inhibitor of tissue calcification. In a previous study, we observed the positive association between abnormal concentrations of uncarboxylated MGP species and increased mortality risk in stable vascular patients. We explore whether co-incidence of abnormal status of uncarboxylated MPG and heart failure (HF) affects the mortality risk. METHODS: We examined 799 patients (mean age 65.1 years) with stable vascular disease and followed them in a prospective study. Both, desphospho-uncarboxylated and total uncarboxylated MGP (dp-ucMGP or t-ucMGP) were quantified by pre-commercial ELISA assays. RESULTS: Elevated (>100 ng/L) circulating brain natriuretic peptide (BNP) and abnormal status of plasma uncarboxylated MGP species (i.e.: dp-ucMGP ≥ 977 pmol/L or t-ucMGP ≤ 2825 nmol/L) were all identified as robust predictors of all-cause 5-year mortality. However, their co-incidence represented a substantial additional risk. We observed the highest mortality risk in patients with elevated BNP plus high dp-ucMGP compared to those with normal BNP plus low dp-ucMGP; fully adjusted HRR's were 4.86 (3.15-7.49). Likewise, the risk was increased when compared with patients with elevated BNP plus low dp-ucMGP; HRR 2.57 (1.60-4.10). Similar result we observed when co-incidence of elevated BNP and low t-ucMGP was analyzed [corresponding HRR's were 4.16 (2.62-6.61) and 1.96 (1.24-3.12)]. CONCLUSIONS: The concomitant abnormality of uncarboxylated MGP and mild elevation of BNP leads in chronic patients with vascular disease to about two-fold increase of the relative mortality risk. We hypothesize that abnormal homeostasis of MGP is involved in the pathophysiology of HF.
- MeSH
- biologické markery krev MeSH
- extracelulární matrix - proteiny krev MeSH
- hodnocení rizik * MeSH
- kalcinóza MeSH
- lidé MeSH
- míra přežití trendy MeSH
- následné studie MeSH
- nemoci cév krev komplikace mortalita MeSH
- prospektivní studie MeSH
- proteiny vázající vápník krev MeSH
- rizikové faktory MeSH
- senioři MeSH
- srdeční selhání krev komplikace mortalita MeSH
- vitamin K MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- multicentrická studie MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika epidemiologie MeSH
The aim of this study was to compare the levels of nesfatin-1 in healthy subjects with those in prediabetic and diabetic patients who have different glucose tolerance levels. Overall, 100 subjects were divided into 5 groups healthy control (C), impaired fasting glycemia (IFG), impaired glucose tolerance (IGT), metabolic syndrome (MS) and type 2 diabetes mellitus, (Type 2 DM). Glycated hemoglobin (HbA1c) assessed the glycemic control. Homeostasis model assessment of insulin resistance (HOMA-IR) was determined using computer analyses. Nesfatin-1 levels were measured using ELISA method. IFG and IGT (prediabetic groups) from MS and Type 2 DM (diabetic groups) differed significantly in HOMA-IR. The nesfatin-1 levels were lower, although not statistically significant, in IFG (0.937+/-0.03 ng/ml, p=0.07) and IGT (1.039+/-0.06 ng/ml, p=0.5) groups compared to healthy subjects (1.094+/-0.07 ng/ml). However, the nesfatin-1 levels were lower in patients with Type 2 DM (0.867+/-0.02 ng/ml, p=0.007) and MS (0.885+/-0.01 ng/ml, p=0.01) compared to healthy subjects. Nesfatin-1 levels were significantly lower in diabetic patients compared to healthy subjects. This study supports the role of insulin resistance in decreased nesfatin-1 levels in patients with Type 2 DM and MS.
- MeSH
- diabetes mellitus 2. typu krev MeSH
- DNA vazebné proteiny krev MeSH
- dospělí MeSH
- glukózový toleranční test * MeSH
- glykovaný hemoglobin analýza MeSH
- inzulinová rezistence MeSH
- krevní glukóza analýza metabolismus MeSH
- lidé MeSH
- metabolický syndrom krev MeSH
- porucha glukózové tolerance krev MeSH
- prediabetes krev MeSH
- proteiny nervové tkáně krev MeSH
- proteiny vázající vápník krev MeSH
- zdraví dobrovolníci pro lékařské studie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Matrix Gla protein (MGP), a natural inhibitor of calcification, strongly correlates with the extent of coronary calcification. Vitamin K is the essential cofactor for the activation of MGP. The nonphosphorylated-uncarboxylated isoform of MGP (dp-ucMGP) reflects the status of this vitamin. We investigated whether there is an association between dp-ucMGP and stiffness of elastic and muscular-type large arteries in a random sample from the general population. In a cross-sectional design, we analyzed 1087 subjects from the Czech post-MONICA study. Aortic and femoro-popliteal pulse wave velocities (PWVs) were measured using a Sphygmocor device. Dp-ucMGP concentrations were assessed in freshly frozen samples by enzyme-linked immunosorbent assay methods using the InaKtif MGP iSYS pre-commercial kit developed by IDS and VitaK. Aortic PWV significantly (P<0.0001) increased across the dp-ucMGP quartiles. After adjustment for all potential confounders, aortic PWV independently correlated with dp-ucMGP (with beta coefficient (s.d.) 11.61 (5.38) and P-value=0.031). In a categorized manner, subjects in the top quartile of dp-ucMGP (⩾ 671 pmol l(-1)) had a higher risk of elevated aortic PWV, with corresponding adjusted odds ratio (95% confidence interval) 1.73 (1.17-2.5). In contrast, no relation between dp-ucMGP and femoro-popliteal PWV was found. In conclusion, increased dp-ucMGP, which is a circulating biomarker of vitamin K status and vascular calcification, is independently associated with aortic stiffness, but not with stiffness of distal muscular-type arteries.
- MeSH
- analýza pulzové vlny MeSH
- biologické markery krev MeSH
- dospělí MeSH
- ELISA MeSH
- extracelulární matrix - proteiny krev MeSH
- fosforylace MeSH
- lidé středního věku MeSH
- lidé MeSH
- lineární modely MeSH
- logistické modely MeSH
- multivariační analýza MeSH
- nemoci aorty krev diagnóza patofyziologie MeSH
- odds ratio MeSH
- onemocnění periferních arterií krev diagnóza patofyziologie MeSH
- proteiny vázající vápník krev MeSH
- průřezové studie MeSH
- rizikové faktory MeSH
- senioři MeSH
- tuhost cévní stěny * MeSH
- upregulace MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
BACKGROUND: Lipoprotein-associated phospholipase A2 (Lp-PLA2) is independently associated with cardiovascular risk, probably via inflammatory activity in sclerotic plaque. We speculated whether Lp-PLA2 has a role in the aetiology of vascular calcifications, estimated from circulating uncarboxylated matrix Gla protein (MGP) species and whether we could find a potential interaction of Lp-PLA2 and MGP in terms of mortality. MATERIALS AND METHODS: We examined 798 patients (mean age 65.1 years) with stable vascular disease and followed them in a prospective study. Both, desphospho-uncarboxylated and total MGP (dp-ucMGP or t-ucMGP) were quantified by pre-commercial ELISA assays, developed by VitaK (Maastricht, The Netherland) RESULTS: Lp-PLA2 activity was independently positively associated with desphospho-uncarboxylated MGP (dp-ucMGP) [β coeff = 0.098, p=0.006]. 1SD of Lp-PLA2 activity was associated with 37% increased risk (p=0.001) of elevated dp-ucMGP (≥977 pmol/L, top quartile). In the Cox proportional hazard model adjusted for conventional risk factors, the patients in the highest quartile of dp-ucMGP or lowest quintile of total-uncarboxylated ucMGP (<2660 nmol/L) had higher risk of all-cause mortality [HRR 2.79 (95% CI 1.97-3.94) and HRR 1.69 (95% CI 1.18-2.42), respectively]. We observed no effect of high Lp-PLA2 activity (≥195 nmol/min/mL) on total mortality. CONCLUSIONS: We assume that Lp-PLA2 is involved in vascular calcification and that dp-ucMGP is a more appropriate biomarker of residual risk than Lp-PLA2 itself.
- MeSH
- 1-alkyl-2-acetylglycerofosfocholinesterasa krev MeSH
- analýza přežití MeSH
- biologické markery krev MeSH
- extracelulární matrix - proteiny krev MeSH
- kardiovaskulární nemoci krev mortalita MeSH
- lidé středního věku MeSH
- lidé MeSH
- proporcionální rizikové modely MeSH
- prospektivní studie MeSH
- proteiny vázající vápník krev MeSH
- rizikové faktory MeSH
- senioři MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Nizozemsko MeSH
BACKGROUND: Vitamin K is the essential co-factor for activation of matrix Gla-protein (MGP), the natural inhibitor of tissue calcification. Biologically inactive, desphospho-uncarboxylated MGP (dp-ucMGP) is a marker of vascular vitamin K status and is described to predict mortality in patients with heart failure and aortic stenosis. We hypothesized that increased dp-ucMGP might be associated with mortality risk in clinically stable patients with chronic vascular disease. MATERIALS AND METHODS: We examined 799 patients (mean age 65.1 ± 9.3 years) who suffered from myocardial infarction, coronary revascularization or first ischemic stroke (pooled Czech samples of EUROASPIRE III and EUROASPIRE-stroke surveys), and followed them in a prospective cohort study. To estimate the 5-year all-cause and cardiovascular mortality we ascertained vital status and declared cause of death. Circulating dp-ucMGP and desphospho-carboxylated MGP (dp-cMGP) were measured by ELISA methods (IDS and VitaK). RESULTS: During a median follow-up of 2050 days (5.6 years) 159 patients died. In the fully adjusted multivariate Cox proportional hazard model, the patients in the highest quartile of dp-ucMGP (≥ 977 pmol/L) had higher risk of all-cause and cardiovascular 5-year mortality [HRR 1.89 (95% CI, 1.32-2.72) and 1.88 (95% CI, 1.22-2.90)], respectively. Corresponding HRR for dp-cMGP were 1.76 (95% CI, 1.18-2.61) and 1.79 (95% CI, 1.12-2.57). CONCLUSIONS: In patients with overt vascular disease, circulating dp-ucMGP and dp-cMGP were independently associated with the risk of all-cause and cardiovascular mortality. Since published results are conflicting regarding the dp-cMGP, we propose only circulating dp-ucMGP as a potential biomarker for assessment of additive cardiovascular risk.
- MeSH
- biologické markery krev MeSH
- cévní mozková příhoda krev mortalita MeSH
- ELISA MeSH
- extracelulární matrix - proteiny krev chemie MeSH
- infarkt myokardu krev mortalita MeSH
- ischemická choroba srdeční krev mortalita MeSH
- Kaplanův-Meierův odhad MeSH
- kardiovaskulární nemoci krev diagnóza MeSH
- lidé středního věku MeSH
- lidé MeSH
- nemoci cév krev mortalita MeSH
- proporcionální rizikové modely MeSH
- prospektivní studie MeSH
- proteiny vázající vápník krev chemie MeSH
- regresní analýza MeSH
- revaskularizace myokardu mortalita MeSH
- senioři MeSH
- vitamin K metabolismus MeSH
- výsledek terapie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
BACKGROUND: Predicting the long-term outcome after traumatic brain injury (TBI) is an important component of treatment strategy. Despite dramatically improved emergency management of TBI and apparent clinical recovery, most patients with TBI still may have long-term central nervous system (CNS) impairment. METHODS: Sixty-three patients with TBI were enrolled into the prospective study. Venous blood samples were taken at admission and every 24 h for a maximum of 6 consecutive days. Serum concentrations of the biomarkers S100B, neuron-specific enolase (NSE), GFAP, NF-H, secretagogin and Hsp70 were quantified immuno-luminometrically or by enzyme-linked immunosorbent assay. The outcome was evaluated 6 months after TBI using the Glasgow Outcome Scale (GOS) in all patients. RESULTS: The S100B levels in patients with worse outcome (GOS 4 or death) were already significantly higher at D0 (p < 0.001; p = 0.002). NSE levels were significantly higher in patients who died or had worse outcomes (p < 0.001; p = 0.003). Patients who had worse outcomes (GOS) or died had higher GFAP values (p < 0.001; p < 0.001), but their dynamics were similar over the same period. NF-H grew significantly faster in patients who had a worse GOS or died (p < 0.001; p = 0.001). CONCLUSIONS: Although further prospective study is warranted, these findings suggest that levels of biomarkers correlate with mortality and may be useful as predictors of outcome in children with TBI.
- MeSH
- biologické markery krev MeSH
- dítě MeSH
- gliový fibrilární kyselý protein krev MeSH
- kojenec MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- neurofilamentové proteiny krev MeSH
- neurotrofní faktory krev MeSH
- novorozenec MeSH
- poranění mozku krev mortalita terapie MeSH
- prediktivní hodnota testů MeSH
- předškolní dítě MeSH
- prospektivní studie MeSH
- proteiny S100 krev MeSH
- proteiny tepelného šoku HSP70 krev MeSH
- proteiny vázající vápník krev MeSH
- transportní proteiny krev MeSH
- výsledek terapie MeSH
- Check Tag
- dítě MeSH
- kojenec MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- novorozenec MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
V poslední době narůstají informace o možnosti stanovení přítomnosti a rozsahu mozkové leze vyšetřením určitých parametrů v žilní krvi (napr. S-100). Tento způsob diagnostiky by mohl významně urychlit a zlepsit diagnostiku poškození CNS. Cílem práce bylo stanovení diagnostické validity (senzitivity a specificity) vyšetření S-100 v žilní krvi u pacientů s mozkovou cévní příhodou, resp. jakoukoliv lézí CNS. Bylo vyšetřeno 34 probandů - pacientů neurologického oddělení Nemocnice Šternberk. Jedinci byli rozděleni podle přítomnosti postižení CNS na dvě skupiny (ischemická CMP / bez ischemické CMP a jakékoliv postižení CNS / bez jakéhokoliv postižení CNS). U všech osob bylo provedeno CT vyšetření mozku a stanovena koncentrace SIOO (EC2010). Osoby s ischemickou CMP (n = 16) měly hodnoty S-100 významně vyšší než osoby bez ní (n = 18) (mediány 0,16 vs 0,06, p = 0,01). Osoby s jakoukoliv afekcí CNS (n = 22) měly hodnoty S-100 taktéž významně vyšší než osoby bez této afekce (n = 12) (mediány 0,11 vs 0,06, p = 0,03). Senzitivita pro přítomnost ischemické CMP činila 63 %, specificita 92 % při koncentraci S-100 > 0,1 μg/l (AUC ROC 0,79; 95 % CI 0,6 - 0,92). Senzitivita pro přítomnost jakékoliv afekce CNS činila 50 %, specificita 88 % při koncentraci S-100 >0,1 gA (AUC ROC 0,76; 95 % CI 0,57 - 0,9). Stanovení SlOO v žilní krvi vykazuje dostatečnou diagnostickou efektivitu pro odhad přítomnosti poškození CNS.
Recentiy, the number of information about the possibilities of determining the presence and extent of a cerebral lesion by means of examining certain parameters in venous blood (e.g. S-100) has been increasing. This diagnostic method could significantiy accelerate and improve the diagnostics of the central nervous system (CNS) damage. The report aimed at determining the diagnostic validity (sensitivity and spedficity) of examining S-100 in venous blood of patients with cerebral vascular accident, or any other CNS lesion. Thirty-four probands - patients of the Department of Neurology, Hospital in Šternberk, were investigated. According to the CNS involvement, they were divided into two groups (ischemic cerebrovascular accident - CVA/without ischemic CVA, and any CNS involvemení/without any CNS involvement). In all the subjects a CT examination of the brain was carried out and SIOO concentration (EC2010) was determined. In persons with ischemic CVA (n = 16) the values of S-100 were significantly higher if compared with those without CVA (n = 18) (medians 0.16 vs 0.06, p = 0.01). Patients with any involvement of the CNS (n = 22) also showed significantly higher levels of S-100 in comparison with subjects without this affection (n = 12) (medians 0.11 vs 0.06, p = 0.03). Sensitivity for the presence of ischemic CVA was 63%, specificity 92% at the concentration of S-100 > 0.1, μg/1, (AUC ROC 0.79, 95% CI 0.6 - 0.92). Sensitivity for the presence of any CNS afí-ection was 50%, specificity 88% at the concentration of S-100 > 0.1, |ig/l (AUC ROC 0.76,95%, CI 0.57 - 0.9). The determination of S 100 in venous blood has shown sufficient diagnostic efficiency for the estimation of the presence of a CNS affection.
- Klíčová slova
- S100,
- MeSH
- cévní mozková příhoda diagnóza MeSH
- lidé MeSH
- nemoci centrálního nervového systému diagnóza MeSH
- proteiny vázající vápník analýza krev MeSH
- Check Tag
- lidé MeSH
