Laser-induced breakdown spectroscopy (LIBS) is a promising technique for the readout of immunochemical assays utilizing indirect detection of labels (Tag-LIBS), typically based on nanoparticles. We have previously demonstrated that Tag-LIBS immunoassay employing yttrium-based photon-upconversion nanoparticles (UCNPs) can reach sensitivity similar to commonly used enzyme and fluorescence immunoassays. In this study, we report on further increasing the sensitivity of UCNP-based Tag-LIBS immunoassay by employing magnetic microbeads (MBs) as the solid phase in the determination of cancer biomarker prostate-specific antigen. Due to the possibility of analyte preconcentration, MBs enabled achieving a limit of detection (LOD) of 4.0 pg·mL-1, representing two orders of magnitude improvement compared with equivalent microtiter plate-based assay (LOD of 460 pg·mL-1). In addition, utilizing MBs opens up the possibility of an internal standardization of the LIBS readout by employing iron spectral lines, which improves the assay robustness by compensating for LIBS signal fluctuations and bead-bound immunocomplexes lost throughout the washing steps. Finally, the practical applicability of the technique was confirmed by the successful analysis of clinical samples, showing a strong correlation with the standard electrochemiluminescence immunoassay. Overall, MB-based Tag-LIBS was confirmed as a promising immunoassay approach, combining fast readout, multiplexing possibilities, and high sensitivity approaching upconversion luminescence scanning while avoiding the requirement of luminescence properties of labels.

• MeSH
• imunoanalýza metody   MeSH
• lasery * MeSH
• lidé MeSH
• limita detekce * MeSH
• mikrosféry MeSH
• prostatický specifický antigen * analýza   imunologie   krev   MeSH
• spektrální analýza metody   MeSH
• ytrium chemie   účinky záření   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Sborník obsahující eseje, reportáže a rozhovory, které se zabývají tématem eutanazie a možných rizik. Určeno odborné i široké veřejnosti.

• MeSH
• eutanazie MeSH
• klinická etika MeSH
• postoj ke smrti MeSH
• Publikační typ
• populární práce MeSH
• sborníky MeSH

• Konspekt
• Etika. Morální filozofie
• NLK Obory
• etika, bioetika, lékařská etika

Publikace se zaměřuje na perioperační a anesteziologickou péči. Určeno odborné veřejnosti, zejména praktickým lékařům.

• MeSH
• anestezie MeSH
• klinické lékařství MeSH
• perioperační medicína MeSH
• perioperační období MeSH
• perioperační péče MeSH
• praktické lékařství MeSH

• Konspekt
• Patologie. Klinická medicína
• NLK Obory
• chirurgie
• všeobecné lékařství
• NLK Publikační typ
• kolektivní monografie

Příručka, která se zaměřuje na symptomy dlouhého COVIDu a jejich zvládání pacientem. Obsahuje i případové studie. Určeno široké veřejnosti.

• MeSH
• chronická nemoc MeSH
• dlouhodobá péče MeSH
• péče o sebe MeSH
• postakutní syndrom COVID-19 * MeSH
• Publikační typ
• kazuistiky MeSH
• populární práce MeSH
• příručky MeSH

• Konspekt
• Patologie. Klinická medicína
• NLK Obory
• zdravotní výchova
• pneumologie a ftizeologie

• MeSH
• fúzní onkogenní proteiny genetika   MeSH
• lidé MeSH
• nádorové biomarkery MeSH
• nádory měkkých tkání * MeSH
• protein EWS vázající RNA MeSH
• protein FUS vázající RNA MeSH
• sarkom * diagnóza   genetika   MeSH
• transkripční faktory NFATC MeSH
• transkripční faktory metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• dopisy MeSH

Viral infection may represent a stress condition to the host cell. Cells react to it by triggering the defence programme to restore homeostasis and these events may in turn impact the viral replication. The knowledge about tick-borne encephalitis virus (TBEV) infection-associated stress is limited. Here we investigated the interplay between TBEV infection and stress pathways in PMJ2-R mouse macrophage cell line, as macrophages are the target cells in early phases of TBEV infection. First, to determine how stress influences TBEV replication, the effect of stress inducers H2O2 and tunicamycin (TM) was tested. Viral multiplication was decreased in the presence of both stress inducers suggesting that the stress and cellular stress responses restrict the virus replication. Second, we investigated the induction of oxidative stress and endoplasmic reticulum (ER) stress upon TBEV infection. The level of oxidative stress was interrogated by measuring the reactive oxygen species (ROS). ROS were intermittently increased in infected cells at 12 hpi and at 72 hpi. As mitochondrial dysfunction may result in increased ROS level, we evaluated the mitochondrial homeostasis by measuring the mitochondrial membrane potential (MMP) and found that TBEV infection induced the hyperpolarization of MMP. Moreover, a transient increase of gene expression of stress-induced antioxidative enzymes, like p62, Gclm and Hmox1, was detected. Next, we evaluated the ER stress upon TBEV infection by analysing unfolded protein responses (UPR). We found that infection induced gene expression of two general sensors BiP and CHOP and activated the IRE1 pathway of UPR. Finally, since the natural transmission route of TBEV from its tick vector to the host is mediated via tick saliva, the impact of tick saliva from Ixodes ricinus on stress pathways in TBEV-infected cells was tested. We observed only marginal potentiation of UPR pathway. In conclusion, we found that TBEV infection of PMJ2-R cells elicits the changes in redox balance and triggers cellular stress defences, including antioxidant responses and the IRE1 pathway of UPR. Importantly, our results revealed the negative effect of stress-evoked events on TBEV replication and only marginal impact of tick saliva on stress cellular pathways.

• MeSH
• buněčné linie MeSH
• klíšťová encefalitida * MeSH
• myši MeSH
• peroxid vodíku metabolismus   MeSH
• protein-serin-threoninkinasy metabolismus   MeSH
• reaktivní formy kyslíku metabolismus   MeSH
• replikace viru MeSH
• viry klíšťové encefalitidy * genetika   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

Induction therapy followed by CD34+ cell mobilisation and autologous transplantation represents standard of care for multiple myeloma (MM). However, the anti-CD38 monoclonal antibodies daratumumab and isatuximab have been associated with mobilisation impairment, yet the mechanism remains unclear. In this study, we investigated the effect of three different regimens (dara-VCd, isa-KRd and VTd) on CD34+ cells using flow cytometry and transcriptomics. Decreased CD34+ cell peak concentration and yields, longer collection and delayed engraftment were reproduced after dara-VCd/isa-KRd versus VTd induction in 34 patients in total. Using flow cytometry, we detected major changes in the proportion of apheresis product and bone marrow CD34+ subsets in patients treated with regimens containing anti-CD38 therapy; however, without any decrease in CD38high B-lymphoid progenitors in both materials. RNA-seq of mobilised CD34+ cells from 21 patients showed that adhesion genes are overexpressed in CD34+ cells after dara-VCd/isa-KRd and JCAD, NRP2, MDK, ITGA3 and CLEC3B were identified as potential target genes. Finally, direct in vitro effect of isatuximab in upregulating JCAD and CLEC3B was confirmed by quantitative PCR. These findings suggest that upregulated adhesion-related interactions, rather than killing of CD34+ cells by effector mechanisms, could be leading causes of decreased mobilisation efficacy in MM patients treated with anti-CD38 therapy.

• MeSH
• antigeny CD34 analýza   MeSH
• antigeny CD38 MeSH
• kostní dřeň chemie   MeSH
• lidé MeSH
• mnohočetný myelom * terapie   MeSH
• mobilizace hematopoetických kmenových buněk MeSH
• průtoková cytometrie MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

Health effects of vegan diets among children and adolescents are a controversial public health topic. Thus, the aim of the present systematic review is to evaluate a broad range of health outcomes among vegan children and adolescents aged 0 to 18 years. 18 studies met the inclusion criteria (17 cross-sectional, 1 RCT). Meta-analyses showed lower protein, calcium, vitamin B2, saturated fatty acid, and cholesterol intakes, and lower ferritin, HDL and LDL levels as well as height in vegan compared to omnivorous children/adolescents. Higher intakes of carbohydrates, polyunsaturated fatty acids, fiber, folate, vitamins C and E, magnesium, iron, and potassium were observed in vegans. Blood levels of vitamin B12 were higher among vegan children due to supplement use. Single study results suggested further differences between vegan and non-vegan children, such as lower bone mineral content or urinary iodine among vegan children. Risk of Bias was rated as high or very high in 7 out of 18 studies. The certainty of evidence for the meta-analyses was low (n = 2) or very low (n = 46). Overall, the available evidence points to both risks and benefits associated with a vegan diet among children, although more and better designed studies are needed.

• MeSH
• dieta veganská * MeSH
• dítě MeSH
• fyziologie výživy dětí MeSH
• kojenec MeSH
• lidé MeSH
• mladiství MeSH
• nutriční stav MeSH
• potravní doplňky MeSH
• předškolní dítě MeSH
• Check Tag
• dítě MeSH
• kojenec MeSH
• lidé MeSH
• mladiství MeSH
• předškolní dítě MeSH
• Publikační typ
• časopisecké články MeSH
• metaanalýza MeSH
• přehledy MeSH
• systematický přehled MeSH

• Publikační typ
• novinové články MeSH

• Publikační typ
• novinové články MeSH
• rozhovory MeSH