Green processing
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- MeSH
- duševní procesy MeSH
- lidé MeSH
- neuroplasticita * MeSH
- videohry * psychologie MeSH
- výzkum MeSH
- Check Tag
- lidé MeSH
- MeSH
- fyziologický stres MeSH
- lidé MeSH
- neurobehaviorální symptomy MeSH
- psychický stres MeSH
- Check Tag
- lidé MeSH
152 s. : il. ; 20 cm
Kniha o přírodní léčbě rozedmy plic a chronických obstrukčních plicních nemocí ukazuje, že alternativní holistické terapie, sahající od bylinek až po homeopatii, nabízejí ve zmírňování ochromujících příznaků souvisejících s CHOPN slibnou budoucnost. Green nám již od počátečního nástinu fyziologie dýchacího procesu představuje komplexní program, který zahrnuje detoxikaci, změny jídelníčku, potravní doplňky a bylinnou medicínu; dýchací techniky a cvičení, jako jsou aerobik, jóga, čchi-kung a tai-či; a alternativní metody jako je homeopatie, akupunktura a masáže. Knihu doporučujeme všem pacientům trpícím CHOPN, jejich rodinám, všem odborníkům a vlastně komukoli, kdo hledá nástroje, jak si pomoci sám, a alternativy k doplnění obvyklých léčebných metod zaměřených na CHOPN.
- MeSH
- chronická obstrukční plicní nemoc diagnóza rehabilitace terapie MeSH
- cvičení MeSH
- dechová cvičení MeSH
- dýchací soustava anatomie a histologie MeSH
- emfyzém diagnóza rehabilitace terapie MeSH
- fytoterapie MeSH
- fyziologie dýchací soustavy MeSH
- komplementární terapie metody MeSH
- odvykání kouření MeSH
- potravní doplňky MeSH
- Publikační typ
- populární práce MeSH
12, 420 s. : il.
Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle and RNA polymerase II mediated transcription. Several pharmacological CDK inhibitors are currently in clinical trials as potential cancer therapeutics and some of them also exhibit antiviral effects. Olomoucine II and roscovitine, purine-based inhibitors of CDKs, were described as effective antiviral agents that inhibit replication of a broad range of wild type human viruses. Olomoucine II and roscovitine show high selectivity for CDK7 and CDK9, with important functions in the regulation of RNA polymerase II transcription. RNA polymerase II is necessary for viral transcription and following replication in cells. We analyzed the effect of inhibition of CDKs by olomoucine II on gene expression from viral promoters and compared its effect to widely-used roscovitine. We found that both roscovitine and olomoucine II blocked the phosphorylation of RNA polymerase II C-terminal domain. However the repression of genes regulated by viral promoters was strongly dependent on gene localization. Both roscovitine and olomoucine II inhibited expression only when the viral promoter was not integrated into chromosomal DNA. In contrast, treatment of cells with genome-integrated viral promoters increased their expression even though there was decreased phosphorylation of the C-terminal domain of RNA polymerase II. To define the mechanism responsible for decreased gene expression after pharmacological CDK inhibitor treatment, the level of mRNA transcription from extrachromosomal DNA was determined. Interestingly, our results showed that inhibition of RNA polymerase II C-terminal domain phosphorylation increased the number of transcribed mRNAs. However, some of these mRNAs were truncated and lacked polyadenylation, which resulted in decreased translation. These results suggest that phosphorylation of RNA polymerase II C-terminal domain is critical for linking transcription and posttrancriptional processing of mRNA expressed from extrachromosomal DNA.
- MeSH
- buněčné linie MeSH
- buněčný cyklus účinky léků MeSH
- Cercopithecus aethiops MeSH
- cyklin-dependentní kinasy antagonisté a inhibitory MeSH
- DNA virů MeSH
- fosforylace účinky léků MeSH
- inhibitory proteinkinas farmakologie MeSH
- ledviny účinky léků metabolismus MeSH
- lidé MeSH
- posttranskripční úpravy RNA účinky léků MeSH
- promotorové oblasti (genetika) účinky léků MeSH
- puriny farmakologie MeSH
- RNA-polymerasa II genetika metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
... Green; 5. BCL-2 family proteins and the control of mitochondrial apoptosis Anthony Letai; 6. ... ... Green; 5. BCL-2 family proteins and the control of mitochondrial apoptosis Anthony Letai; 6. ...
1st ed. xiii, 421 s. : il., [32] s. barev. příl. ; 29 cm
- MeSH
- apoptóza fyziologie MeSH
- buněčná smrt MeSH
- Publikační typ
- monografie MeSH
- Konspekt
- Buněčná biologie. Cytologie
- NLK Obory
- cytologie, klinická cytologie
- biologie
A synchronous population of cells is one of the prerequisites for studying cell cycle processes such as DNA replication, nuclear and cellular division. Green algae dividing by multiple fission represent a unique single cell system enabling the preparation of highly synchronous cultures by application of a light-dark regime similar to what they experience in nature. This chapter provides detailed protocols for synchronization of different algal species by alternating light-dark cycles; all critical points are discussed extensively. Moreover, detailed information on basic analysis of cell cycle progression in such cultures is presented, including analyses of nuclear, cellular, and chloroplast divisions. Modifications of basic protocols that enable changes in cell cycle progression are also suggested so that nuclear or chloroplast divisions can be followed separately.
- MeSH
- barvení a značení metody MeSH
- buněčné dělení MeSH
- buněčné kultury metody MeSH
- buněčný cyklus MeSH
- Chlamydomonas reinhardtii cytologie genetika růst a vývoj MeSH
- Chlorophyta cytologie genetika růst a vývoj MeSH
- chloroplasty genetika MeSH
- DNA rostlinná genetika MeSH
- fotoperioda * MeSH
- frakcionace buněk metody MeSH
- replikace DNA MeSH
- světlo MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Catalytic properties and high adsorption affinity of nucleic acids and proteins to silver amalgam electrode surface make this kind of electrified interface perspective for bioanalytical and biomedical applications. For the first time, a basal-plane pyrolytic graphite electrode (bPGE) has been used as a substrate for electrodeposition of silver amalgam particles (AgAPs). Optimization of the resulting composition, surface morphology and electrochemical properties of the AgAPs was done by scanning electron microscopy with energy disperse X-ray spectroscopy, image processing software and voltammetric detection of electrochemically reducible model organic nitro compound, 4-nitrophenol. Spectro-electrochemical applicability of bPGE-AgAP has been demonstrated by electrolysis of 4-nitrophenol. Simultaneous UV-Vis-chronoamperometry provided information on the number of exchange electrons and the reduction rate constants. Preferential adsorption of the fluorescently labelled calf thymus DNA and the green fluorescent protein (GFP) on the surface of AgAPs was observed by fluorescence microscopy. In contrast to previously studied indium-tin oxide and vapour-deposited gold decorated by AgAPs, herein the presented bPGE-AgAP has provided sufficiently wide negative potential window allowing direct electroanalysis of non-labelled DNA and GFP using intrinsic electrochemical signals independently of the fluorescent labelling. The bPGE-AgAP can thus be expected to find application opportunities in protein electrochemistry, (bio)sensor development or in-situ spectro-electrochemical studies.
