Malárie je prakticky nejzávažnějším tropickým onemocněním. V České republice je možné občas diagnostikovat malárii u osob, které pobývaly v tropech a subtropech. Plasmodium falciparum je ze 4 druhů původců malárie člověka nejnebezpečnější. V roce 1999 byl na infekční klinice FN Hradec Králové hospitalizován pacient s infekcí Plasmodium falciparum po převozu ze Súdánu. Průběh onemocnění, včetně poklesu parazitémie z 10,5 %, objevení se gametocytů, vymizení plasmodií a uzdravení pacienta je popsáno a doplněno rovněž fotografickou dokumentací.
Malaria is practically the most important parasitic dinase. In the Czech Republic sometimes we can see malarik parasites from persons which travelled tropical and subtropical countries. Of all the human malaria parasites (four species), Plasmodium falciparum is the most highly pathogenic. We report a case of one patient with Plasmodium falciparum after travelling Sudan. A course of dinase, parasitemia from 10,5 % (ring forms of the young trophozoites and also gametocytes in the microphotographs of the blood smears) to the negative results after specific treatment are written.
- MeSH
- Malaria blood therapy MeSH
- Parasitemia parasitology MeSH
- Plasmodium falciparum pathogenicity MeSH
- Check Tag
- Male MeSH
- Publication type
- Case Reports MeSH
... Plasmodium genome papers - complete genome sequences of two plasmodium parasites and their proteomic ...
Nature
1 elektronický optický disk (CD-ROM) : barev. ; 13 cm
- Conspectus
- Biochemie. Molekulární biologie. Biofyzika
- NML Fields
- biologie
- parazitologie
- cestovní a tropická medicína
- NML Publication type
- CD-ROM
American journal of tropical medicine and hygiene ; Supplement Vol. 50. 4
[1st ed.] 74 s. : obr., tab., grafy ; 24 cm
- MeSH
- Malaria prevention & control MeSH
- Plasmodium falciparum MeSH
- Protozoan Vaccines MeSH
- Publication type
- Congress MeSH
- Conspectus
- Patologie. Klinická medicína
- NML Fields
- urgentní lékařství
- alergologie a imunologie
- parazitologie
Malaria transmission is a serious emergence in urban and semiurban areas worldwide, becoming a major international public health concern. Malaria is transmitted through the bites of Anopheles mosquitoes. The extensive employ of synthetic pesticides leads to negative effects on human health and the environment. Recently, plant-synthesized nanoparticles have been proposed as highly effective mosquitocides. In this research, we synthesized silver nanoparticles (AgNP) using the Azadirachta indica seed kernel extract as reducing and stabilizing agent. AgNP were characterized by UV-vis spectrophotometry, SEM, EDX, XRD and FTIR spectroscopy. The A. indica seed kernel extract was toxic against Anopheles stephensi larvae and pupae, LC50 were 232.8ppm (larva I), 260.6ppm (II), 290.3ppm (III), 323.4ppm (IV), and 348.4ppm (pupa). AgNP LC50 were 3.9ppm (I), 4.9ppm (II), 5.6ppm (III), 6.5ppm (IV), and 8.2ppm (pupa). The antiplasmodial activity of A. indica seed kernel extract and AgNP was evaluated against CQ-resistant (CQ-r) and CQ-sensitive (CQ-s) strains of Plasmodium falciparum. IC50 of A. indica seed kernel extract were 63.18μg/ml (CQ-s) and 69.24μg/ml (CQ-r). A. indica seed kernel-synthesized AgNP achieved IC50, of 82.41μg/ml (CQ-s) and 86.12μg/ml (CQ-r). However, in vivo anti-plasmodial experiments conducted on Plasmodium berghei infecting albino mice showed moderate activity of the A. indica extract and AgNP. Overall, this study showed that the A. indica-mediated fabrication of AgNP is of interest for a wide array of purposes, ranging from IPM of mosquito vectors to the development of novel and cheap antimalarial drugs.
- MeSH
- Anopheles drug effects growth & development parasitology MeSH
- Antimalarials pharmacology MeSH
- Azadirachta chemistry metabolism MeSH
- Metal Nanoparticles chemistry MeSH
- Pupa drug effects parasitology MeSH
- Larva drug effects parasitology MeSH
- Malaria parasitology prevention & control MeSH
- Plasmodium berghei drug effects MeSH
- Plasmodium falciparum drug effects MeSH
- Silver pharmacology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
Comparison of diagnostic methods for Plasmodium spp. in humans from Uganda and the Central African Republic showed that parasites can be efficiently detected by PCR in fecal samples. These results, which rely solely on PCR-based examination of feces, validate numerous estimates of the prevalence of malaria in great apes.
- MeSH
- Cytochromes b genetics MeSH
- Molecular Diagnostic Techniques MeSH
- Feces parasitology MeSH
- Humans MeSH
- Plasmodium falciparum genetics isolation & purification MeSH
- Polymerase Chain Reaction MeSH
- Protozoan Proteins genetics MeSH
- Sensitivity and Specificity MeSH
- Malaria, Falciparum diagnosis parasitology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Malaria remains a major health hazard for humans, despite the availability of efficacious antimalarial drugs and other interventions. Given that the disease is often deadly for children under 5 years and pregnant women living in malaria-endemic areas, an efficacious vaccine to prevent transmission and clinical disease would be ideal. Plasmodium, the causative agent of malaria, uses proteases and protease inhibitors to control and process to invade host, modulate host immunity, and for pathogenesis. Plasmodium parasites rely on these proteases for their development and survival, including feeding their metabolic needs and invasion of both mosquito and human tissues, and have thus been explored as potential targets for prophylaxis. In this chapter, we have discussed the potential of proteases like ROM4, SUB2, SERA4, SERA5, and others as vaccine candidates. We have also discussed the role of some protease inhibitors of plasmodium and mosquito origin. Inhibition of plasmodium proteases can interrupt the parasite development at many different stages therefore understanding their function is key to developing new drugs and malaria vaccines.
- MeSH
- Protease Inhibitors pharmacology therapeutic use MeSH
- Humans MeSH
- Malaria drug therapy immunology parasitology prevention & control MeSH
- Plasmodium * drug effects enzymology immunology physiology MeSH
- Peptide Hydrolases * immunology MeSH
- Protozoan Proteins * antagonists & inhibitors immunology MeSH
- Malaria Vaccines * immunology MeSH
- Vaccine Development MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
