Very high-frequency oscillations (VHFOs, > 500 Hz) are more specific in localizing the epileptogenic zone (EZ) than high-frequency oscillations (HFOs, < 500 Hz). Unfortunately, VHFOs are not visible in standard clinical stereo-EEG (SEEG) recordings with sampling rates of 1 kHz or lower. Here we show that "shadows" of VHFOs can be found in frequencies below 500 Hz and can help us to identify SEEG channels with a higher probability of increased VHFO rates. Subsequent analysis of Logistic regression models on 141 SEEG channels from thirteen patients shows that VHFO "shadows" provide additional information to gold standard HFO analysis and can potentially help in precise EZ delineation in standard clinical recordings.
- MeSH
- Electroencephalography * MeSH
- Humans MeSH
- Stereotaxic Techniques MeSH
- Blood Coagulation Tests MeSH
- High-Frequency Ventilation * MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
For a better understanding of plant nutrition processes, it is important to study the flux of nutrients within plants. However, existing xylem sap sampling methods are typically destructive and do not allow for repeated, highly frequent measurements of nutrient concentration. In this paper, we present a novel use of microdialysis (MD) for characterizing xylem sap phosphate (PO43-) concentration as a possible alternative to destructive sampling. First, MD probes were tested under laboratory conditions in vitro, in a stirred solution test, and in vivo, using beech tree stem segments. Exponential decline in the relative recovery (RR) with an increasing MD pumping rate allows for determining an optimal sampling interval (i.e., the maximum amount of sample volume with the minimum required concentration). The RR changed only minimally, with a change in the simulated sap flow velocity during the in vivo stem segment test. This suggests that MD can be applied over a range of naturally occurring sap flow velocities. Differences in the ionic strength between the xylem sap and the perfusate pumped through the MD did not influence the RR. Then, MD was successfully applied in a 24 h field campaign in two beech trees of different ages and allowed for in situ assessments of the diurnal variation of PO43- concentration and (together with xylem flow measurements) flux variability in living trees. Both beech trees exhibited the same diurnal pattern in PO43- concentrations with higher concentrations in the younger tree. The xylem PO43- concentration measured with MD was in the same order of magnitude as that received through destructive sampling in the younger tree. The MD probes did not show a decline in RR after the field application. We showed that MD can be applied to capture the PO43- concentration dynamics in the xylem sap with bihourly resolution under field conditions.
- MeSH
- Phosphorus * MeSH
- Laboratories * MeSH
- Microdialysis MeSH
- Trees MeSH
- Xylem MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Allele frequencies for 17 short tandem repeats (STRs) autosomal loci (D2S1338, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D19S433, D21S11, CSF1PO, FGA, PentaD, PentaE, TH01, TPOX, vWA) were studied in an extensive sample (max. N=1411) of unrelated individuals originating from the Czech Republic. Population and forensic parameters were estimated. Except for FGA and Penta E loci, no deviations from the Hardy-Weinberg equilibrium were detected. A comparative analysis with published data revealed significant differences in allele frequencies for some loci from the Polish population and three Hungarian populations (Ashkenazim population and Romany populations from Debrecen and Baranya County, respectively). A combination of these 17 STR loci provides a powerful tool for forensic identification in the native Czech population.
- MeSH
- DNA Fingerprinting MeSH
- Gene Frequency MeSH
- Humans MeSH
- Polymerase Chain Reaction MeSH
- Genetics, Population MeSH
- Tandem Repeat Sequences MeSH
- Check Tag
- Humans MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
Před zahájením léčby jsme odebrali periferní krev 60 nemocným s karcinomem hrtanu a hltanu. Z krve byly separovány CD4 lymfocyty. Sledovali jsme jejich schopnost adherence k povrchu v závislosti na přítomnos¬ti antigenu (test inhibice adherence leukocytů - LAI assay). Jako antigen byl použit specifický antigen, vyro¬bený z příslušné nádorové tkáně (mitochondriální antigen nádorové buňky) a nespecifický antigen, vyrobený ze séra inbredních myší kmene C3H/H2K, infikovaných LDH virem (kapsidový antigen). Výsledky byly hod¬noceny pomocí non-adherence indexu (NAI). Pokus byl opakován při expozici substrátu magnetickému sinu-soidnímu poli (MSP) síťového kmitočtu o indukci 1 a 10 mT. Výsledky byly vyhodnoceny stejným způsobem a porovnány pomocí t-testu pro 2 nezávislé výběry. Výsledky měření ukázaly, že CD4 lymfocyty získané z krve pacientů s karcinomy hrtanu a hltanu mají, oproti CD4 lymfocytům zdravých lidí, sníženou schopnost adherence k povrchu (vyšší hodnoty NAI). Po expozici MSP síťového kmitočtu se však jejich schopnost adhe-rovat statisticky významně zvyšuje. Za předpokladu, že schopnost adherence lymfocytů je projevem buňka¬mi mediované imunity (CMI), lze konstatovat, že MSP ovlivňuje CMI, a to v případě MSP síťového kmitoč¬tu o indukci 1 i 10 mT pozitivním způsobem.
Prior to treatment, we háve sampled blood from 60 patients suffering carcinoma of the larynx and pharynx. CD4 lymphocytes were separated from the blood. We háve followed up their capacity of surface adherence depending on the presence of antigen (leucocyte adherence inhibition assay). As antigen there served a spe-cific antigen prepared from the corresponding tumor tissue (mitochondrial antigen of the tumor cell) and a nonspecific antigen prepared from the sérum of inbred C3H/H2K strain mice infected with LDH virus (cap-sid antigen). The results were evaluated with the aid of the non-adherence index (NAI). The experiment was repeated under exposure of the substráte to a magnetic sinusoid field (MSF) of power frequency at an induc-tion of 1 and 10 mT. The results were evaluated in like manner and compared through the t-test for two inde¬pendent series. Results of the measurings showed that CD4 lymphocytes obtained from the blood of patients suffering carcinomas of the larynx and of the pharynx, as compared to CD4 lymphocytes of healthy subjects, háve a decreased capacity of surface adherence (higher NAI values). Upon exposure to MSF of power net¬work frequency their capacity to adhere increased to a statistically significant degree. On presumption that the adherence capacity of lymphocytes is a manifestation of cell-mediated immunity (CMI), it can be stated that MSF influences CMI in a positive sense, námely in the čase of MSF of power network frequency at an induction of 1 and 10 mT.
- MeSH
- Antigens, Neoplasm radiation effects MeSH
- Electromagnetic Fields MeSH
- Research Support as Topic MeSH
- Dose-Response Relationship, Immunologic MeSH
- Carcinoma diagnosis etiology MeSH
- Humans MeSH
- Magnetics MeSH
- Head and Neck Neoplasms diagnosis etiology MeSH
- T-Lymphocytes radiation effects MeSH
- Leukocyte Adherence Inhibition Test methods statistics & numerical data MeSH
- Check Tag
- Humans MeSH
BACKGROUND: Oncopanel genomic testing, which identifies important somatic variants, is increasingly common in medical practice and especially in clinical trials. Currently, there is a paucity of reliable genomic reference samples having a suitably large number of pre-identified variants for properly assessing oncopanel assay analytical quality and performance. The FDA-led Sequencing and Quality Control Phase 2 (SEQC2) consortium analyze ten diverse cancer cell lines individually and their pool, termed Sample A, to develop a reference sample with suitably large numbers of coding positions with known (variant) positives and negatives for properly evaluating oncopanel analytical performance. RESULTS: In reference Sample A, we identify more than 40,000 variants down to 1% allele frequency with more than 25,000 variants having less than 20% allele frequency with 1653 variants in COSMIC-related genes. This is 5-100× more than existing commercially available samples. We also identify an unprecedented number of negative positions in coding regions, allowing statistical rigor in assessing limit-of-detection, sensitivity, and precision. Over 300 loci are randomly selected and independently verified via droplet digital PCR with 100% concordance. Agilent normal reference Sample B can be admixed with Sample A to create new samples with a similar number of known variants at much lower allele frequency than what exists in Sample A natively, including known variants having allele frequency of 0.02%, a range suitable for assessing liquid biopsy panels. CONCLUSION: These new reference samples and their admixtures provide superior capability for performing oncopanel quality control, analytical accuracy, and validation for small to large oncopanels and liquid biopsy assays.
- MeSH
- Alleles * MeSH
- Gene Frequency * MeSH
- Genetic Heterogeneity MeSH
- Genetic Variation * MeSH
- Genetic Testing methods standards MeSH
- Genomics methods standards MeSH
- Humans MeSH
- Biomarkers, Tumor * MeSH
- Cell Line, Tumor MeSH
- Neoplasms diagnosis genetics MeSH
- Workflow MeSH
- DNA Copy Number Variations MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
PURPOSE: The aim of this proof-of-concept study is to introduce new high-dynamic ECG technique with potential to detect temporal-spatial distribution of ventricular electrical depolarization and to assess the level of ventricular dyssynchrony. METHODS: 5-kHz 12-lead ECG data was collected. The amplitude envelopes of the QRS were computed in an ultra-high frequency band of 500-1000 Hz and were averaged (UHFQRS). UHFQRS V lead maps were compiled, and numerical descriptor identifying ventricular dyssynchrony (UHFDYS) was detected. RESULTS: An electrical UHFQRS maps describe the ventricular dyssynchrony distribution in resolution of milliseconds and correlate with strain rate results obtained by speckle tracking echocardiography. The effect of biventricular stimulation is demonstrated by the UHFQRS morphology and by the UHFDYS descriptor in selected examples. CONCLUSIONS: UHFQRS offers a new and simple technique for assessing electrical activation patterns in ventricular dyssynchrony with a temporal-spatial resolution that cannot be obtained by processing standard surface ECG. The main clinical potential of UHFQRS lies in the identification of differences in electrical activation among CRT candidates and detection of improvements in electrical synchrony in patients with biventricular pacing.
- MeSH
- Adult MeSH
- Ventricular Dysfunction, Left diagnostic imaging therapy MeSH
- Echocardiography, Three-Dimensional methods MeSH
- Electrocardiography methods MeSH
- Image Interpretation, Computer-Assisted * MeSH
- Middle Aged MeSH
- Humans MeSH
- Ventricular Remodeling physiology MeSH
- Aged MeSH
- Cardiac Resynchronization Therapy methods MeSH
- Severity of Illness Index MeSH
- Treatment Outcome MeSH
- Sampling Studies MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
The single nucleotide polymorphism (SNP) A118G (rs1799971) in the Mu Opioid Receptor 1 (OPRM1) gene is associated with significant variations in analgesic doses and adverse effects of opioids. The A118G OPRM1 allele distributions vary significantly between different populations worldwide. The study aimed to assess the allele frequency and genotype distribution of OPRM1 A118G SNP in Saudis. This cross-sectional study included 124 healthy Saudis (62 males and 62 females) visiting the King Abdulaziz University Hospital in Jeddah, Saudi Arabia. The Oragene®-DISCOVER (OGR-600) kits were used to collect saliva samples from the participants. Polymerase chain reaction-restriction fragment length polymorphism was utilized to assess the SNP. Among the tested population, 79.03% (95% C.I. 70.81-85.82) were homozygous wild-type A118A, 16.13% (95% C.I. 10.14-23.80) were heterozygous A118G, and 4.84% (95% C.I. 1.80-10.23) were homozygous mutant G118G. OPRM1 A118G polymorphism allele frequencies were 87% (95% C.I. 79.89-92.44) and 13% (95% C.I. 7.56-20.11) for the 118A and 118G alleles, respectively. A higher frequency of the OPRM1 118G allele was present in females, 21% (95% C.I. 11.66-33.17) compared to males, 5% (95% C.I. 1.01-13.50). Relative to other Asian countries, the Saudi population showed a low prevalence of the OPRM1 A118G polymorphism, with a higher frequency of the 118G allele in females. Our research will contribute to the existing knowledge on the prevalence of OPRM1 A118G polymorphism, which could be considered for the personalized prescribing of opioid analgesics.
- MeSH
- Gene Frequency MeSH
- Genotype MeSH
- Polymorphism, Single Nucleotide * genetics MeSH
- Humans MeSH
- Analgesics, Opioid * MeSH
- Cross-Sectional Studies MeSH
- Receptors, Opioid, mu genetics MeSH
- Receptors, Opioid MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Saudi Arabia MeSH
BACKGROUND AND AIMS: The frequency and timing of meals may affect cardiovascular health (CVH) outcomes, but large-scale epidemiological studies are lacking. The aim of this study was to understand the relationship between eating time interval and frequency, and measures of ideal CVH in the Kardiovize Brno cohort study, a random urban sample population in Central Europe. METHODS AND RESULTS: 1659 members of the Kardiovize Brno 2030 cohort were included in a cross-sectional study (mean age = 46.86 years; 44.6% male). Exposure variables were eating time interval and frequency, and skipping meals. Primary outcomes were indices of CVH, including body mass index, diet, physical activity, smoking, blood pressure, glucose and cholesterol, and the composite CVH score. Cluster analysis and binary logistic regression analysis were used to evaluate eating habits and the association between variables. After adjustment for well-known risk factors, subjects who skipped breakfast or the afternoon snack had a higher risk of poor CVH (OR = 1.613; 95%CI = 1.121-2.320; p = 0.010; OR = 1.409; 95%CI = 1.110-1.788; p = 0.005, respectively). Moreover, we identified three clusters of individuals based on eating habits; from cluster 1 to cluster 3, eating time interval and frequency increased and this was associated with increases in CVH score from 8.70 (SEM = 0.10) in cluster 1, and 9.06 (SEM = 0.08) in cluster 2 to 9.42 (SEM = 0.09) in cluster 3 (p-trend = 0.019). CONCLUSIONS: Our findings suggest that skipping breakfast or the afternoon snack are risk factors for poor CVH, while higher eating time interval and frequency may promote ideal CVH.
- MeSH
- Time Factors MeSH
- Risk Reduction Behavior * MeSH
- Adult MeSH
- Meals * MeSH
- Cardiovascular Diseases diagnosis epidemiology prevention & control MeSH
- Middle Aged MeSH
- Humans MeSH
- Protective Factors MeSH
- Eating * MeSH
- Prospective Studies MeSH
- Cross-Sectional Studies MeSH
- Risk Factors MeSH
- Feeding Behavior * MeSH
- Urban Health * MeSH
- Health Status MeSH
- Healthy Lifestyle * MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Geographicals
- Czech Republic MeSH
Cíl: Cévní mozková příhoda je velkou zdravotní zátěží, která způsobuje úmrtnost a invaliditu dospělých. Kognitivní porucha je po mrtvici běžná a tento stav může ovlivnit kvalitu života a nezávislost pacienta. V této studii jsme zkoumali souvislost mezi frek- vencí recidivy mrtvice a kognitivní funkcí. Metodika: Tato studie je průřezová. Ke sběru dat o celkem 69 pacientech s mrtvicí z nemocnice University of Muhammadiyah Malang (UMM) byla použita účelová technika odběru vzorků. Kritériem pro zařazení byli pacienti s cévní mozkovou příhodou, kteří zaznamenali recidivu, byli při vědomí a byli schopni komunikovat alespoň verbálně. Stanovení počtu vzorků bylo provedeno pomocí softwaru G*Power s velikostí účinku 0,35, p-hodnotou 0,05 a silou 80 %. Data byla sbírána pomocí sociodemografického dotazníku a minipsychologického vyšetření. Data byla analyzována pomocí deskriptivní statistiky a Spearmanovy rho korelace kvůli porušenému předpokladu normálního rozdělení. Výsledky: Průměrný věk účastníků byl 61,91 (12,65) let a 84,10 % z nich mělo ischemickou cévní mozkovou příhodu. Nejvyšší výskyt recidivy cévní mozkové příhody byl první recidivou (63 %) a většina respondentů měla středně těžkou kognitivní poruchu (52,18 %). Existuje souvislost mezi recidivou mozkové mrtvice a kognitivními funkcemi s nízkou korelací (p = 0,017, r = –0,29). Závěr: Výsledky této studie ukazují, že kognitivní funkce budou sníženy recidivou mrtvice. Zdravotnické týmy by měly vyvinout strategie a intervenční protokoly pro zlepšení dodržování kontrolních faktorů pacienta, které mohou způsobit recidivu cévní mozkové příhody, aby bylo možné minimalizovat pokles kognitivních funkcí.
Aim: Stroke is a major health burden that causes adult mortality and disability. Cognitive impairment is common after stroke, and this condition can affect the patient's quality of life and independence. In this study, we investigated the association between stroke recurrence frequency and cognitive function. Methods: The study design was a cross-sectional study. A purposive sampling technique was used to collect data on a total of 69 stroke patients from the University of Muhammadiyah Malang (UMM) Hospital. The inclusion criteria were stroke patients who experienced recurrence, were conscious, and able to communicate at least verbally. The determination of the number of samples was made using G*Power software with an effect size of 0.35, p-value of 0.05, and power of 80%. Data was collected using a sociodemographic questionnaire and mini-mental state examination. Data were analyzed using descriptive statistics and Spearman's rho correlation due to the violated normal distribution assumption. Results: The mean age of the participants was 61.91 (12.65) years, and 84.10% of them had ischemic stroke. The highest incidence of stroke recurrence was the first recurrence (63%), and the majority of respondents had moderate cognitive impairment (52.18%). There is an association between stroke recurrence and cognitive function, with a low correlation (p = 0.017, r = -0.29). Conclusions: The results of this study demonstrate that cognitive function will be decreased by stroke recurrence. Healthcare teams should develop strategies and intervention protocols to improve patient compliance in controlling factors that can cause stroke recurrence, so that cognitive function decline can be minimized.
