Příspěvek se zaměřuje na spektrum terapeutických materiálů, které jsou vhodné k léčbě ran, ulcerací a lézí v genitoanální oblasti u žen. Přestože obsah článku se věnuje genitoanální oblasti u žen, předložená lokální terapie se používá ve stejné oblasti rovněž u mužů. Histologicky ženský a mužský genitál má shodné tkáně, které se rozlišují pouze anatomickými rysy. Poškození v genitoanální oblasti vyžadují materiály k opakované aplikaci během dne, cenově přijatelné, zmírňující bolest a zlepšující komfort pacientů. Názorné schéma v tabulce s terapeutickými materiály je rozděleno do tří oddílů, zahrnujících fázi zánětu s vysoušením spodiny rány, fázi podporující čištění ran a fázi regenerační. Do poškození v této oblasti lze zahrnout i iritační a postradiační dermatitis. Předložená kazuistika popisuje komplikovanou léčbu lézí v genitoanální oblasti u nespolupracující pacientky.
This article deals with the issue of the spectrum of therapeutic materials suitable for the treatment of wounds, ulcerations and lesions in the genitoanal area in women. Despite the fact that the content of the article is primarily dedicated to the female genitoanal area, the described local therapy is also applicable to men in the same region. Histologically, the female and male genitalia consist of identical tissues, which are distinguished only by anatomical features. Damage to the genitoanal area requires materials for repeated application during the day, are affordable, relieve pain and improve comfort of patients. The illustrative scheme in the table with therapeutic materials is divided into three sections, including the inflammation phase with drying of the wound bed, the phase supporting wound cleansing and the regeneration phase. Damage in this area may also involve irritant and post-radiation dermatitis. The presented case describes the complicated treatment of lesions in the genitoanal area of an uncooperative patient.
- MeSH
- Wound Healing * drug effects MeSH
- Hydrogels administration & dosage MeSH
- Urinary Incontinence pathology MeSH
- Dermatitis, Contact therapy MeSH
- Middle Aged MeSH
- Humans MeSH
- Ointments administration & dosage MeSH
- Radiodermatitis therapy MeSH
- Solutions administration & dosage MeSH
- Vascular Surgical Procedures methods MeSH
- Genitalia, Female * pathology injuries MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Female MeSH
Skin represents the largest organ in the human body, functioning as a protective barrier against environmental factors while playing a critical role in thermoregulation. Acne vulgaris is recognized as the most common dermatological condition affecting adolescents, and if left untreated, it can result in lasting skin damage and associated psychosocial challenges. This study aims to develop innovative polymeric biomaterials that could effectively support the treatment of acne vulgaris. The synthesis of these biomaterials involves the use of polyethylene glycol 6000, sodium alginate, and the antioxidant protein glutathione (GHS) to create polymeric hydrogels. These hydrogels were generated via a UV-mediated crosslinking process. To enhance the functional properties of the hydrogels, zinc oxide microparticles (ZnO), synthesized through a wet precipitation method, were incorporated into the formulations. Characterization of the ZnO was performed using Fourier-Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), particle sizer analysis, and Scanning Electron Microscopy (SEM). Additionally, the bioactivity of the synthesized materials was evaluated through incubation in media simulating physiological body fluids. The cytotoxic effects of the biomaterials were assessed using an indirect test on mouse fibroblast (L929) cells, in accordance with ISO 10993-5 guidelines. The results of our research indicate that the developed biomaterials exhibit potential as a carrier for active substances, contributing positively to the treatment of acne vulgaris and potentially improving overall skin health.
- MeSH
- Acne Vulgaris drug therapy MeSH
- Alginates chemistry MeSH
- Biocompatible Materials chemistry pharmacology MeSH
- Cell Line MeSH
- Fibroblasts drug effects metabolism MeSH
- Glutathione * metabolism MeSH
- Hydrogels * chemistry MeSH
- Skin * drug effects metabolism MeSH
- Humans MeSH
- Mice MeSH
- Drug Carriers chemistry MeSH
- Zinc Oxide * chemistry pharmacology MeSH
- Regeneration drug effects MeSH
- Spectroscopy, Fourier Transform Infrared MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Free radical polymerization technique was used to formulate Poloxamer-188 based hydrogels for controlled delivery. A total of seven formulations were formulated with varying concentrations of polymer, monomer ad cross linker. In order to assess the structural properties of the formulated hydrogels, Fourier Transform Infrared Spectroscopy (FTIR), Thermogravimetric analysis (TGA), Differential Scanning Calorimetry (DSC), Scanning electron microscopy (SEM), and X-ray diffraction (XRD) were carried out. To assess the effect of pH on the release of the drug from the polymeric system, drug release studies were carried in pH 1.2 and 7.4 and it was found that release of the drug was significant in pH 7.4 as compared to that of pH 1.2 which confirmed the pH responsiveness of the system. Different kinetic models were also applied to the drug release to evaluate the mechanism of the drug release from the system. To determine the safety and biocompatibility of the system, toxicity study was also carried out for which healthy rabbits were selected and formulated hydrogels were orally administered to the rabbits. The results obtained suggested that the formulated poloxamer-188 hydrogels are biocompatible with biological system and have the potential to serve as controlled drug delivery vehicles.
- MeSH
- Acrylic Resins * chemistry MeSH
- Calorimetry, Differential Scanning MeSH
- X-Ray Diffraction MeSH
- Hydrogels * chemistry MeSH
- Hydrogen-Ion Concentration MeSH
- Rabbits MeSH
- Drug Delivery Systems MeSH
- Delayed-Action Preparations chemistry pharmacokinetics MeSH
- Microscopy, Electron, Scanning MeSH
- Drug Carriers chemistry MeSH
- Poloxamer * chemistry MeSH
- Spectroscopy, Fourier Transform Infrared MeSH
- Thermogravimetry MeSH
- Timolol * administration & dosage pharmacokinetics chemistry MeSH
- Drug Liberation MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
OBJECTIVE: This research aims to design and evaluate an enteric-coated hard capsule dosage form for targeted delivery of biological materials, such as FMT (fecal microbiota transplant) or live microbes, to the distal parts of the GIT. The capsules are designed to be internally protected against destruction by hydrophilic filling during passage through the digestive tract. METHODS: Hard gelatin capsules and DRcapsTMcapsules based on HPMC and gellan were used to encapsulate a hydrophilic body temperature-liquefying gelatin hydrogel with caffeine or insoluble iron oxide mixture. Different combinations of polymers were tested for the internal (ethylcellulose, Eudragit® E, and polyvinyl acetate) and external (Eudragit® S, Acryl-EZE®, and cellacefate) coating. The external protects against the acidic gastric environment, while the internal protects against the liquid hydrophilic filling during passage. Coated capsules were evaluated using standard disintegration and modified dissolution methods for delayed-release dosage forms. RESULTS: Combining suitable internal (ethylcellulose 1.0 %) and external (Eudragit® S 20.0 %) coating of DRcapsTM capsules with the wiping and immersion method achieved colonic release times. While most coated capsules met the pharmaceutical requirements for delayed release, one combination stood out. Colonic times were indicated by the dissolution of soluble caffeine (during 120-720 min) measured by the dissolution method, and capsule rupture was indicated by the release of insoluble iron oxide (after 480 min) measured by the disintegration method. This promising result demonstrates the composition's suitability and potential to protect the content until it's released, inspiring hope for the future of colon-targeted delivery systems and its potential for the pharmaceutical and biomedical fields. CONCLUSION: Innovative and easy capsule coatings offer significant potential for targeted drugs, especially FMT water suspension, to the GIT, preferably the colon. The administration method is robust and not considerably affected by the quantity of internal or external coatings. It can be performed in regular laboratories without specialized individual and personalized treatment equipment, making it a practical and feasible method for drug delivery.
- MeSH
- Polysaccharides, Bacterial chemistry MeSH
- Biocompatible Materials chemistry MeSH
- Cellulose * chemistry analogs & derivatives MeSH
- Hypromellose Derivatives chemistry MeSH
- Hydrophobic and Hydrophilic Interactions * MeSH
- Hydrogels chemistry MeSH
- Caffeine chemistry administration & dosage MeSH
- Colon * metabolism MeSH
- Polymethacrylic Acids chemistry MeSH
- Drug Delivery Systems * methods MeSH
- Delayed-Action Preparations chemistry MeSH
- Polymers chemistry MeSH
- Polyvinyls chemistry MeSH
- Capsules * MeSH
- Drug Liberation * MeSH
- Gelatin * chemistry MeSH
- Ferric Compounds chemistry administration & dosage MeSH
- Publication type
- Journal Article MeSH
This study develops and characterizes novel biodegradable soft hydrogels with dual porosity based on N-(2-hydroxypropyl)methacrylamide (HPMA) copolymers cross-linked by hydrolytically degradable linkers. The structure and properties of the hydrogels are designed as scaffolds for tissue engineering and they are tested in vitro with model mesenchymal stem cells (rMSCs). Detailed morphological characterization confirms dual porosity suitable for cell growth and nutrient transport. The dual porosity of hydrogels slightly improves rMSCs proliferation compared to the hydrogel with uniform pores. In addition, the laminin coating supports the adhesion of rMSCs to the hydrogel surface. However, hydrogels modified by heptapeptide RGDSGGY significantly stimulate cell adhesion and growth. Moreover, the RGDS-modified hydrogels also affect the topology of proliferating rMSCs, ranging from single-cell to multicellular clusters. The 3D reconstruction of the hydrogels with cells obtained by laser scanning confocal microscopy (LSCM) confirms cell penetration into the inner structure of the hydrogel and its corresponding microstructure. The prepared biodegradable oligopeptide-modified hydrogels with dual porosity are suitable candidates for further in vivo evaluation in soft tissue regeneration.
- Keywords
- kontaktologie,
- MeSH
- Hydrogels MeSH
- Contact Lenses, Hydrophilic history MeSH
- Ophthalmology MeSH
- Publication type
- Biography MeSH
- Interview MeSH
- Geographicals
- Czechoslovakia MeSH
This article presents a method for producing hydrogel dressings using high methylated pectin from apples or citrus, doped with the antiseptic agent, octenidine dihydrochloride. Octenidine was incorporated in-situ during the polymer crosslinking. The pectins were characterized by their varying molecular weight characteristics, monosaccharide composition, and degree of esterification (DE). The study assessed the feasibility of producing biologically active hydrogels with pectin and delved into how the polymer's characteristics affect the properties of the resulting dressings. The structure evaluation of hydrogel materials showed interactions between individual components of the system and their dependence on the type of used pectin. Both the antimicrobial properties and cytotoxicity of the dressings were evaluated. The results suggest that the primary determinants of the functional attributes of the hydrogels are the molecular weight characteristics and the DE of the pectin. As these values rise, there is an increase in polymer-polymer interactions, overshadowing polymer-additive interactions. This intensification strengthens the mechanical and thermal stability of the hydrogels and enhances the release of active components into the surrounding environment. Biological evaluations demonstrated the ability of octenidine to be released from the dressings and effectively inhibit the growth of microbial pathogens.
- MeSH
- Anti-Infective Agents, Local * chemistry pharmacology MeSH
- Citrus chemistry MeSH
- Hydrogels * chemistry pharmacology MeSH
- Imines * chemistry MeSH
- Humans MeSH
- Malus chemistry MeSH
- Microbial Sensitivity Tests MeSH
- Molecular Weight MeSH
- Bandages * MeSH
- Pectins * chemistry pharmacology MeSH
- Pyridines * chemistry pharmacology MeSH
- Staphylococcus aureus drug effects MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Polysaccharides like hyaluronan (HA) and chondroitin sulfate (CS) are native of the brain's extracellular matrix crucial for myelination and brain maturation. Despite extensive research on HA and CS as drug delivery systems (DDS), their high water solubility limits their application as drug carriers. This study introduces an injectable DDS using aldehyde-modified hyaluronic acid (HAOX) hydrogel containing polyelectrolyte complexes (PEC) formed with calcium, gelatin, and either CS or aldehyde-modified CS (CSOX) to deliver minocycline for Multiple Sclerosis therapy. PECs with CSOX enable covalent crosslinking to HAOX, creating immobilized PECs (HAOX_PECOX), while those with CS remain unbound (HAOX_PECS). The in situ forming DDS can be administered via a 20 G needle, with rapid gelation preventing premature leakage. The system integrates into an implanted device for minocycline release through either Fickian or anomalous diffusion, depending on PEC immobilization. HAOX_PECOX reduced burst release by 88 %, with a duration of 127 h for 50 % release. The DDS exhibited an elastic modulus of 3800 Pa and a low swelling ratio (0-1 %), enabling precise control of minocycline release kinetics. Released minocycline reduced IL-6 secretion in the Whole Blood Monocytes Activation Test, suggesting that DDS formation may not alter the biological activity of the loaded drug.
- MeSH
- Aldehydes chemistry MeSH
- Chondroitin Sulfates * chemistry MeSH
- Hydrogels * chemistry pharmacology MeSH
- Interleukin-6 metabolism MeSH
- Hyaluronic Acid * chemistry MeSH
- Drug Delivery Systems methods MeSH
- Humans MeSH
- Minocycline * chemistry pharmacology administration & dosage MeSH
- Drug Carriers * chemistry MeSH
- Polyelectrolytes * chemistry MeSH
- Drug Liberation MeSH
- Gelatin * chemistry MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Postoperative distant metastasis and high recurrence rate causes a dilemma in treating triple-negative breast cancer (TNBC) owing to its unforeseeable invasion into various organs or tissues. The wealth of nutrition provided by vascular may facilitate the proliferation and angiogenesis of cancer cells, which further enhance the rates of postoperative metastasis and recurrence. Chemotherapy, as a systemic postoperative adjuvant therapy, is generally applied to diminish recurrence and metastasis of TNBC. Herein, an halofuginone-silver nano thermosensitive hydrogel (HTPM&AgNPs-gel) was prepared via a physical swelling method. The in vitro anticancer efficacy of HTPM&AgNPs-gel was analyzed by investigating cell proliferation, migration, invasion, and angiogenesis capacity. Furthermore, the in vivo anti-cancer activity of HTPM&AgNPs-gel was further appraised through the tumor suppression, anti-metastatic, anti-angiogenic, and anti-inflammatory ability. The optimized HTPM&AgNPs-gel, a thermosensitive hydrogel, showed excellent properties, including syringeability, swelling behavior, and a sustained release effect without hemolysis. In addition, HTPM&AgNPs-gel was confirmed to effectively inhibit the proliferation, migration, invasion, and angiogenesis of MDA-MB-231 cells. An evaluation of the in vivo anti-tumor efficacy demonstrated that HTPM&AgNPs-gel showed a stronger tumor inhibition rate (68.17%) than did HTPM-gel or AgNPs-gel used alone and exhibited outstanding biocompatibility. Notably, HTPM&AgNPs-gel also inhibited lung metastasis induced by residual tumor tissue after surgery and further blocked angiogenesis-related inflammatory responses. Taken together, the suppression of inflammation by interdicting the blood vessels adjoining the tumor and inhibiting angiogenesis is a potential strategy to attenuate the recurrence and metastasis of TNBC. HTPM&AgNPs-gel is a promising anticancer agent for TNBC as a local postoperative treatment.
- MeSH
- Quinazolinones * chemistry administration & dosage pharmacology MeSH
- Hydrogels * administration & dosage chemistry MeSH
- Metal Nanoparticles administration & dosage chemistry MeSH
- Humans MeSH
- Mice, Inbred BALB C MeSH
- Mice, Nude MeSH
- Mice MeSH
- Cell Line, Tumor MeSH
- Neovascularization, Pathologic drug therapy MeSH
- Piperidines * pharmacology administration & dosage chemistry MeSH
- Cell Movement drug effects MeSH
- Cell Proliferation * drug effects MeSH
- Antineoplastic Agents * administration & dosage pharmacology chemistry MeSH
- Silver * chemistry administration & dosage MeSH
- Triple Negative Breast Neoplasms * drug therapy MeSH
- Xenograft Model Antitumor Assays MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
