Spondyloarthritis (SpA) constitute a group of chronic inflammatory immune-mediated rheumatic diseases characterized by genetic, clinical, and radiological features. Recent efforts have concentrated on identifying biomarkers linked to axial SpA associated with inflammatory bowel disease (IBD), offering predictive insights into disease onset, activity, and progression. Genetically, the significance of the HLA-B27 antigen is notably diminished in ankylosing spondylitis (AS) associated with IBD, but is heightened in concurrent sacroiliitis. Similarly, certain polymorphisms of endoplasmic reticulum aminopeptidase (ERAP-1) appear to be involved. Carriage of variant NOD2/CARD15 polymorphisms has been demonstrated to correlate with the risk of subclinical intestinal inflammation in AS. Biomarkers indicative of pro-inflammatory activity, including C-reactive protein (CRP) along with erythrocyte sedimentation rate (ESR), are among the consistent predictive biomarkers of disease progression. Nevertheless, these markers are not without limitations and exhibit relatively low sensitivity. Other promising markers encompass IL-6, serum calprotectin (s-CLP), serum amyloid (SAA), as well as biomarkers regulating bone formation such as metalloproteinase-3 (MMP-3) and Dickkopf-related protein 1 (DKK-1). Additional candidate indicators of structural changes in SpA patients include matrix metalloproteinase-3 (MMP-3), vascular endothelial growth factor (VEGF), tenascin C (TNC), and CD74 IgG. Fecal caprotein (f-CLP) levels over long-term follow-up of AS patients have demonstrated predictive value in anticipating the development of IBD. Serologic antibodies characteristic of IBD (ASCA, ANCA) have also been compared; however, results exhibit variability. In this review, we will focus on biomarkers associated with both axial SpA and idiopathic intestinal inflammation, notably enteropathic spondyloarthritis.
- MeSH
- ankylózující spondylitida krev diagnóza imunologie MeSH
- axiální spondyloartritida krev diagnóza MeSH
- biologické markery * krev MeSH
- C-reaktivní protein analýza metabolismus MeSH
- HLA-B27 antigen genetika imunologie MeSH
- idiopatické střevní záněty * krev imunologie diagnóza komplikace MeSH
- leukocytární L1-antigenní komplex krev MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
Cíl studie: posouzení využitelnosti sérového kalprotektinu jako biomarkeru bakteriální infekce v rutinní praxi. Typ studie: prospektivní, observační studie. Název a sídlo pracoviště: Ústřední vojenská nemocnice – Vojenská fakultní nemocnice Praha Materiál a metody: do studie byli zařazeni všichni pacienti přijatí na Kliniku infekčních nemocí v období leden až březen 2020. Sérový kalprotektin byl stanoven pomocí systému EVOLIS™ Microplate a komerčně dostupné enzymoimunoeseje firmy Biovendor. Výsledky: celkem byla vyhodnocena data 36 nemocných (24 mužů a 12 žen s průměrným věkem 63,9 let), u kterých byla potvrzena diagnóza virové nebo bakteriální infekce. Nejčastějším zdrojem bakteriální infekce byly dýchací cesty (33,3 %), následované měkkými tkáněmi (25 %) a infekcemi urogenitálního a gastrointestinálního traktu (shodně 16,7 %). Virovou infekci nejčastěji představovala chřipka A (66,6 %). Medián sérové koncentrace kalprotektinu u bakteriální infekce byl 4,12 mg/L oproti 2,03 mg/L při infekci virové. U 12 nemocných s bakteriální infekcí a zvýšeným C-reaktivním proteinem (CRP), u kterých nebyl zvýšen prokalcitonin (PCT), byla zjištěna zvýšená hodnota sérového kalprotektinu. Naopak u osmi nemocných s potvrzenou bakteriální infekcí nebyl kalprotektin zvýšen, čtyři z těchto pacientů měli současně s CRP zvýšený i PCT a čtyři nemocní měli zvýšené pouze CRP. V souboru byl rovněž případ bakteriální infekce se zvýšeným kalprotektinem a negativním CRP i PCT. Závěr: výsledky naší studie naznačily význam sérového kalprotektinu jako doplňkového parametru pro diagnostiku bakteriální infekce.
Objective: evaluation of clinical use of serum calprotectin as biomarker of bacterial infection Design: prospective, observational study Settings: Military University Hospital Prague Material and Methods: all patients admitted to the Department of infectious Diseases during the period between January and March 2020 were enrolled to the study. Serum calprotectin was analyzed using EVOLIS™ Microplate system and commercially available assay from the Biovendor company. Results: Altogether, data of 36 enrolled patients with proven bacterial or viral infection (24 males and 12 females with mean age 63.9 years) were evaluated. The most frequent source of bacterial infection was respiratory tract (33.3 %) followed by soft tissue (25 %) and infections of urogenital and gastrointestinal tracts (both 16.7 %). The most common viral infection was flu A (66.6 %). Median of calprotectin serum concentration in bacterial infection was 4.12 mg/L in comparison to 2.03 mg/L in viral infection. In 12 patients with bacterial infection with negative procalcitonin (PCT) levels, both C-reactive protein (CRP) and calprotectin were elevated. On the other hand, eight patients with proven bacterial infection had negative calprotectin serum levels. Four of these patients had elevated CRP and PCT and four CRP only. In addition, in the cohort with bacterial infection, there was one patient with calprotectin elevation only. Conclusion: the results of our study indicated serum calprotectin as additional parameter of bacterial infection.
Background: Psoriasis is a chronic systemic inflammatory disease associated with a wide range of comorbidities, including metabolic syndrome (MetS). Serum calprotectin, ANGPTL8, and oxidative damage to nucleic acids might be associated with both diseases. The presented study describes the influence of psoriasis and MetS on the serum levels of markers of systemic inflammation (calprotectin and ANGPTL8) and markers of oxidative damage to nucleic acids. The applicability of serum levels of calprotectin and ANGPTL8 for monitoring of the activity of psoriasis (diagnostic markers) is also evaluated. Methods: Clinical examination (PASI score, MetS), enzyme-linked immunosorbent assay (ELISA), and Enzyme Immunoassay (EIA). Serum calprotectin, ANGPTL8, 8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanosine, and 8-hydroxyguanine. Results and Conclusions. The psoriasis significantly increased the serum level of calprotectin and the serum level of oxidative damage to nucleic acids, however not the serum level of ANGPTL8. The presence of MetS did not significantly affect the serum levels of calprotectin, ANGPTL8, and oxidative damage to nucleic acids in either psoriasis patients or controls. It seems that the serum level of calprotectin (but not the serum level of ANGPTL8) could be used as a biomarker for monitoring the activity of psoriasis.
- MeSH
- angiopoetinu podobné proteiny krev MeSH
- biologické markery krev MeSH
- dospělí MeSH
- leukocytární L1-antigenní komplex krev MeSH
- lidé středního věku MeSH
- lidé MeSH
- metabolický syndrom diagnóza MeSH
- mladiství MeSH
- mladý dospělý MeSH
- nukleové kyseliny metabolismus MeSH
- oxidační stres MeSH
- peptidové hormony krev MeSH
- poškození DNA MeSH
- psoriáza diagnóza MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- zánět diagnóza MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
The aim of this study was to evaluate the serum levels of calprotectin and calgranulin C and routine biomarkers in patients with bacterial sepsis (BS). The initial serum concentrations of calprotectin and calgranulin C were significantly higher in patients with BS (n = 66) than in those with viral infections (n = 24) and the healthy controls (n = 26); the level of calprotectin was found to be the best predictor of BS, followed by the neutrophil-lymphocyte count ratio (NLCR) and the level of procalcitonin (PCT). The white blood cell (WBC) count and the NLCR rapidly returned to normal levels, whereas PCT levels normalized later and the increased levels of calprotectin, calgranulin C, and C-reactive protein persisted until the end of follow-up. Our results suggest that the serum levels of calprotectin are a reliable biomarker of BS and that the WBC count and the NLCR are rapid predictors of the efficacy of antimicrobial therapy.
- MeSH
- bakteriální infekce krev diagnóza MeSH
- biologické markery krev MeSH
- C-reaktivní protein analýza MeSH
- dospělí MeSH
- kinetika MeSH
- leukocytární L1-antigenní komplex krev MeSH
- lidé středního věku MeSH
- lidé MeSH
- neutrofily cytologie MeSH
- počet leukocytů MeSH
- počet lymfocytů MeSH
- protein S100A12 krev MeSH
- senioři MeSH
- senzitivita a specificita MeSH
- sepse krev diagnóza MeSH
- virové nemoci krev diagnóza MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Pancreatic tumors and their surgical resection are associated with significant morbidity and mortality, and the biomarkers currently used for these conditions have limited sensitivity and specificity. Because calprotectin and calgranulin C serum levels have been demonstrated to be potential biomarkers of certain cancers and complications of major surgery, the levels of both proteins were tested in the current study in patients with benign and malignant pancreatic tumors that were surgically removed. The baseline serum levels and kinetics of calprotectin and calgranulin C during the 7-day postoperative period were evaluated with immunoassays in 98 adult patients who underwent pancreatic surgery. The baseline serum levels of calprotectin and calgranulin C in patients with malignant (n = 84) and benign tumors (n = 14) were significantly higher (p < 0.01) when compared to those in the healthy controls (n = 26). The serum levels of both proteins were also significantly (p < 0.05) higher in patients with benign tumors than in those with malignant tumors. After surgery, the serum levels of calprotectin and calgranulin C were significantly (p < 0.01) higher than their baseline values, and this elevation persisted throughout the seven days of the follow-up period. Interestingly, starting on day 1 of the postoperative period, the serum levels of both proteins were significantly (p < 0.05) higher in the 37 patients who developed postoperative pancreatic fistulas (POPFs) than in the patients who had uneventful recoveries (n = 61). Moreover, the serum levels of calprotectin and calgranulin C demonstrated a significant predictive value for the development of POPF; the predictive values of these two proteins were better than those of the serum level of C-reactive protein and the white blood cell count. Taken together, the results of this study suggest that calprotectin and calgranulin C serum levels are potential biomarkers for pancreatic tumors, surgical injury to the pancreatic tissue and the development of POPFs.
- MeSH
- biologické markery krev MeSH
- C-reaktivní protein MeSH
- leukocytární L1-antigenní komplex krev MeSH
- lidé MeSH
- nádory slinivky břišní chirurgie MeSH
- pooperační období MeSH
- prospektivní studie MeSH
- protein S100A12 krev MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
AIM: Hepcidin is a central regulator of iron homeostasis. Its production is also influenced by systemic inflammation. The aims of this study were to compare hepcidin levels in paediatric patients newly diagnosed with Crohn's disease (CD) and ulcerative colitis (UC) and to determine the association of hepcidin levels with laboratory and clinical parameters of inflammatory bowel disease (IBD) activity. METHODS: Children with newly diagnosed IBD between January 2012 and September 2016 were enrolled in this comparative cross-sectional study. We analysed levels of serum hepcidin, C-reactive protein, iron, ferritin, soluble transferrin receptors, blood count and faecal calprotectin in all subjects. Serum hepcidin levels were measured by reverse-phase liquid chromatography. The Paediatric Crohn's Disease Activity Index was used to evaluate CD in children, and Paediatric Ulcerative Colitis Activity Index was used for the assessment of UC disease activity. RESULTS: Subjects with CD (n = 53) had significantly higher serum hepcidin levels compared with subjects with UC (n = 23) - 22.6 ng/mL (range 8.5-65.0) versus 6.5 ng/mL (range 2.4-25.8) (P < 0.05). Hepcidin was independently associated with ferritin levels in all IBD patients (P < 0.05). Moreover, there was a significant positive correlation between hepcidin and platelet count (P < 0.05) in children with CD and a negative correlation between hepcidin and faecal calprotectin (P < 0.05) in children with UC. CONCLUSION: Different hepcidin levels between children with newly diagnosed CD and UC suggest the distinct contribution of iron deficiency and/or systemic inflammation to anaemia and may help clinicians choose the best anti-anaemic treatment.
- MeSH
- antiinfekční látky krev MeSH
- C-reaktivní protein analýza MeSH
- dítě MeSH
- feces chemie MeSH
- ferritin krev MeSH
- hepcidiny krev MeSH
- idiopatické střevní záněty diagnóza MeSH
- leukocytární L1-antigenní komplex krev MeSH
- lidé MeSH
- mladiství MeSH
- průřezové studie MeSH
- železo krev MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Approximately half of patients with rheumatoid arthritis (RA) have normal C-reactive protein (CRP) levels. Calprotectin is a promising and likely more specific biomarker of disease activity than conventionally used acute phase reactants. We aimed to analyse the levels of serum calprotectin in RA patients with clinically active disease and with normal/low CRP. A total of 160 RA patients underwent clinical examination (DAS28-ESR and CDAI). The levels of calprotectin were analysed in patients with moderate to high disease activity with normal/low CRP levels and in 32 healthy subjects. The discriminatory capacity of calprotectin to identify clinically active patients in spite of normal/low CRP was assessed using ROC curves. Out of all RA patients, 74/160 (46.3%) were in remission or had low disease activity according to DAS28 and had normal/low CRP levels. However, 51/160 (32%) had normal/low CRP levels despite having moderate to high disease activity. In these patients, calprotectin levels were significantly higher than those in patients who had normal/low CRP and were in remission or showed low disease activity (2.7 ± 1.5 vs. 2.1 ± 1.2 μg/mL, p = 0.043), which differed from those in healthy subjects (2.7 ± 1.5 vs. 1.9 ± 1.2 μg/mL, p = 0.011). The discriminatory capacity for calprotectin to distinguish clinically active vs. inactive disease despite normal/low CRP using AUC of the DAS28 was 0.607 (95% CI 0.503 to 0.711, p = 0.043). The present study demonstrates that calprotectin may reflect inflammatory activity in RA patients where CRP fails to do so.
- MeSH
- biologické markery krev MeSH
- C-reaktivní protein analýza MeSH
- krevní sedimentace MeSH
- leukocytární L1-antigenní komplex krev MeSH
- lidé středního věku MeSH
- lidé MeSH
- referenční hodnoty MeSH
- revmatoidní artritida krev MeSH
- stupeň závažnosti nemoci MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Finsko MeSH
Navzdory obrovskému pokroku v neonatologické péči, a to především v péči o nezralé novorozence, zůstává stále velkým problémem novorozenecká sepse. Novorozeneckou sepsi dělíme na časnou, tzv. „early-onset sepsis“, a pozdní, tzv. „late-onset sepsis“. V tomto přehledovém článku se zaměřujeme především na novorozeneckou sepsi časnou. Včasná diagnostika a zahájení terapie rozhoduje zásadním způsobem o dalším osudu dítěte. Klasická definice sepse se opírá o klinické známky a průkaz patogenu. V klinické praxi je to však problematické, a jsou tedy používány také pomocné laboratorní znaky bakteriální infekce. V přehledu uvádíme stručné informace o běžně užívaných laboratorních znacích. Druhou část sdělení věnujeme novým možnostem diagnostiky, konkrétně vyšetření sérového kalprotektinu a CD64 – povrchovému markeru leukocytů.
Despite the huge progress in neonatology, especially in care of premature infants, newborn sepsis remains a big problem.In neonatology, we talk about early-onset newborn sepsis and late-onset newborn sepsis. This review is focused on early-onsetsepsis. Early diagnosis and initiation of therapy is essential for infant`s good prognosis. Classic definition of sepsis is based onthe clinical signs and proof of pathogen in the blood stream. But, this is problematic in clinical practice, so another laboratorymar kers of bacterial infection are used. In this review article, we discuss commonly used markers. In the second part of thisarticle we introduce new diagnostic possibilities, especially new laboratory markers – calprotectin and CD64 (surface markerof leukocytes).
- Klíčová slova
- kalprotektin,
- MeSH
- biologické markery * krev MeSH
- C-reaktivní protein MeSH
- časná diagnóza MeSH
- interleukin-6 MeSH
- kalcitonin krev MeSH
- klinická studie jako téma MeSH
- leukocytární L1-antigenní komplex krev MeSH
- lidé MeSH
- novorozenec MeSH
- novorozenecká sepse * diagnóza MeSH
- receptory IgG krev MeSH
- senzitivita a specificita MeSH
- Check Tag
- lidé MeSH
- novorozenec MeSH
- Publikační typ
- práce podpořená grantem MeSH
- přehledy MeSH
BACKGROUND: Calprotectin may be a sensitive biomarker of rheumatoid arthritis (RA) disease activity. OBJECTIVES: In the current study, we investigated whether calprotectin is a better biomarker than CRP for predicting clinical activity and ultrasound parameters in patients with RA. METHODS: A total of 160 patients with RA underwent clinical (swollen joint count-SJC, tender joint count-TJC, Disease Activity Score-DAS28, Clinical Disease Activity Index-CDAI, and simplified Disease Activity Index-SDAI) and ultrasound (German US7) examination. Clinical and laboratory measures were correlated with ultrasound findings using Spearman´s correlation coefficient. Differences in serum calprotectin levels in patients with variable disease activity according to the DAS28-ESR and CDAI scores were assessed using ANOVA. Multivariate regression analysis was used to determine the predictive values of calprotectin, CRP and SJC for CDAI and PD US synovitis scores. RESULTS: Serum calprotectin was significantly associated with DAS28-ESR (r = 0.321, p<0.001), DAS28-CRP (r = 0.346, p<0.001), SDAI (r = 0.305, p<0.001), CDAI (r = 0.279, p<0.001) scores and CRP levels (r = 0.556, p<0.001). Moreover, calprotectin was significantly correlated with GS (r = 0.379, p<0.001) and PD synovitis scores (r = 0.419, p<0.001). The multivariate regression analysis showed that calprotectin is a better predictor of the CDAI score and PD US synovitis than CRP. CONCLUSIONS: The results of this study support an additional role of calprotectin in assessing inflammatory activity in patients with RA.
- MeSH
- C-reaktivní protein metabolismus MeSH
- dospělí MeSH
- kohortové studie MeSH
- leukocytární L1-antigenní komplex krev MeSH
- lidé středního věku MeSH
- lidé MeSH
- revmatoidní artritida krev diagnostické zobrazování patologie MeSH
- ultrasonografie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
BACKGROUND: The efficacy of exercise therapy for ankylosing spondylitis (AS) is well-documented, but dearth of information is for non-radiographic axial spondyloarthritis (nr-axSpA). Biomarkers like serum calprotectin, interleukins IL-6, IL-17 and tumour necrosis factor (TNF)-α may reflect the disease activity of axial spondyloarthritis (axSpA). In this study, we investigated clinical and laboratory parameters of both axSpA subgroups in response to intensive physical exercise. METHODS: Altogether, 46 patients with axSpA, characterised according to the Assessment of SpondyloArthritis International Society criteria as having nr-axSpA or AS underwent 6-month exercise programme. Clinical outcomes of disease activity, Bath AS Disease Activity Index (BASDAI), AS Disease Activity Index (ASDAS-CRP), mobility, Bath AS Metrology Index (BASMI) and function, Bath AS Functional Index (BASFI) were evaluated at baseline and at the end of the exercise programme. Serum IL-6 and IL-17, TNF-α and calprotectin were measured via ELISA. The clinical and laboratory data of 29 control axSpA patients were used for the evaluation of the results. RESULTS: In all axSpA patients, the ASDAS-CRP (2.10 ± 0.12 to 1.84 ± 0.11, p <0.01) and BASMI (1.28 ± 0.14 to 0.66 ± 0.84, p <0.0001) improved after 6 months of exercise therapy. There was a significant improvement in the ASDAS-CRP in the nr-axSpA subgroup (2.01 ± 0.19 to 1.73 ± 0.16, p <0.05) and in the BASMI in both, the nr-axSpA and the AS subgroups (1.09 ± 0.12 to 0.47 ± 0.08, p <0.0001 and 1.43 ± 0.24 to 0.82 ± 0.23, p <0.0001, respectively). Both, ASDAS-CRP and BASDAI, were significantly improved in the exercise axSpA group compared to the control axSpA group (mean -0.26 vs. -0.13 and -0.49 vs. 0.12, respectively, all p <0.05). Only calprotectin was significantly reduced after the exercise programme in nr-axSpA and AS patients (from 2379.0 ± 243.20 to 1779.0 ± 138.30 μg/mL and from 2430.0 ± 269.70 to 1816.0 ± 148.20 μg/mL, respectively, all p <0.01). The change in calprotectin was more profound in the axSpA intervention group (mean -604.56) than in the control axSpA (mean -149.28, p <0.05). CONCLUSION: This study demonstrated similar efficacy for an intensive exercise programme in both nr-axSpA and AS patients. A significant decrease in serum calprotectin levels in both subgroups of axSpA patients after the exercise programme reflected an improvement in the disease activity and spinal mobility.
- MeSH
- ankylózující spondylitida krev rehabilitace MeSH
- biologické markery krev MeSH
- dospělí MeSH
- ELISA MeSH
- imunoanalýza MeSH
- leukocytární L1-antigenní komplex krev MeSH
- lidé MeSH
- spondylartritida krev rehabilitace MeSH
- terapie cvičením metody MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- klinické zkoušky MeSH
