A novel Gram-stain-negative, strictly aerobic, rod-shaped, light-yellow-pigmented, and chemo-organoheterotrophic bacterium, designated DF-77T, was isolated from dense mats of filamentous algae collected in March 2004 at Okinawa in Japan. The microorganism grew at 0-2.0% NaCl concentrations (w/v), pH 6.0-9.0, and 20-30 °C. The 16S rRNA gene sequence-based phylogenetic tree demonstrated that the strain DF-77T is a novel member of the family Flavobacteriaceae and was greatly related to Flagellimonas nanhaiensis SM1704T with sequence similarity of 95.5%. The main fatty acids were iso-C15:1 G, iso-C15:0, and iso-C17:0 3-OH, and the only isoprenoid quinone was menaquinone-6. The dominant polar lipids were phosphatidylethanolamine, two unidentified aminolipids, an unidentified phosphoaminolipid, and four unidentified lipids. The genome size of strain DF-77T was 3.60 Mbp with a DNA G + C content of 47.5%. The average nucleotide identity (ANI) value between the genomes of strain DF-77T and its closely related species was 69.8-70.7%. The digital DNA - DNA hybridization (dDDH) value of strain DF-77T with the strain of F. nanhaiensis SM1704T was 16.8%. The genome of the strain DF-77T revealed that it encoded several genes involved in bio-macromolecule degradation, indicating a high potential for producing industrially useful enzymes. Consequently, the strain is described as a new species in the genus Flagellimonas, for which the name Flagellimonas algarum sp. nov., is proposed with the type strain DF-77T (= KCTC 72791T = NBRC 114251T).
- MeSH
- DNA, Bacterial genetics chemistry MeSH
- Flavobacteriaceae * classification isolation & purification genetics MeSH
- Phospholipids analysis MeSH
- Phylogeny MeSH
- Genome, Bacterial MeSH
- Nucleic Acid Hybridization MeSH
- Fatty Acids analysis MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Bacterial Typing Techniques MeSH
- Vitamin K 2 analysis analogs & derivatives MeSH
- Base Composition MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Japan MeSH
A significant increase in interest in new, naturally occurring sources of antioxidants is evident not only in the food industry but also in the pharmaceutical and cosmetic industries. Plant sources such as fruits, both traditional and less common, are often investigated. Goji berries (Lycium barbarum, Lycium chinense, and Lycium ruthenicum) represent fruits rich in polyphenols, especially phenolic acids (38.91 to 455.57 mg/kg FW) and flavonoids, with black goji berries (L. ruthenicum) containing a predominance of anthocyanins (119.60 to 1112.25 mg/kg FW). In this review, a comparison of polyphenol occurrence and content in the orange-red and black berries of L. barbarum, L. chinense, and L. ruthenicum is described. Goji berries represent a valuable source of nutrients and bioactive compounds that manifest a wide range of health-promoting effects. These benefits represent antioxidant, neuroprotective, and cytoprotective impacts, with effects on the metabolic control of glucose and lipids. This review is focused on an overview of the polyphenolic compounds occurring in these fruits, as well as their antioxidant activity and health benefits.
- Publication type
- Journal Article MeSH
- Review MeSH
This study elucidated the impacts of coenzyme Q10 (COQ10) supplementation in a high-fat diet (HFD) on growth, lipid metabolism and mitochondrial function in spotted seabass (Lateolabrax maculatus). Totally five diets were formulated: a diet with normal fat content (11 % lipid, NFD), a HFD (17 % lipid) and three additional diets by supplementing 5, 20 or 80 mg/kg of COQ10 to the HFD. After an 8-week culture period, samples were collected and analysed. The results demonstrated that COQ10 inclusion prevented the HFD-induced deterioration of growth performance and feed utilisation. COQ10 alleviated the deposition of saturated fatty acids following HFD intake and promoted the assimilation of n-3 and n-6 PUFA. Moreover, COQ10 administration inhibited the surge in serum transaminase activity and reduced hepatic lipid content following HFD ingestion, which was consistent with the results of oil red O staining. In addition, HFD feeding led to reduced hepatic citrate synthase and succinate dehydrogenase activities and decreased ATP content. Notably, COQ10 administration improved these indices and up-regulated the expression of mitochondrial biogenesis-related genes (pgc-1α, pgc-1β, nrf-1, tfam) and autophagy-related genes (pink1, mul1, atg5). In summary, supplementing 20-80 mg/kg of COQ10 in the HFD promoted growth performance, alleviated hepatic fat accumulation and enhanced liver mitochondrial function in spotted seabass.
- MeSH
- Diet, High-Fat * adverse effects MeSH
- Liver metabolism drug effects MeSH
- Animal Feed analysis MeSH
- Lipid Metabolism drug effects MeSH
- Mitochondria * drug effects metabolism MeSH
- Bass * growth & development metabolism MeSH
- Dietary Supplements MeSH
- Ubiquinone * analogs & derivatives pharmacology administration & dosage MeSH
- Fatty Liver * veterinary etiology drug therapy prevention & control MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
Východiska: Hledání účinných biomarkerů pro včasnou diagnostiku ovariálního karcinomu (ovarian cancer – OC) patří k naléhavým úkolům moderní onkogynekologie. Metabolické profilování pomocí ultra vysokoúčinné kapalinové chromatografie a hmotnostní spektrometrie (ultraigh performance liquid chromatography and mass spectrometry – UHPLC-MS) poskytuje informace o souhrnu všech nízkomolekulárních metabolitů vzorku biologických tekutin pacienta, které odrážejí procesy probíhající v těle. Cílem studie bylo prozkoumat metabolomický profil krevní plazmy a moči pacientek se serózním ovariálním adenokarcinomem pomocí UHPLC-MS. Materiál a metody: K provedení metabolomické analýzy bylo odebráno 60 vzorků krevní plazmy a 60 vzorků moči pacientek s diagnózou serózního karcinomu vaječníků a 20 vzorků zdravých dobrovolníků. Chromatografická separace byla provedena na chromatografu Vanquish Flex UHPLC System (Thermo Scientific, Německo). Analýza hmotnostní spektrometrií byla provedena na Orbitrap Exploris 480 (Thermo Scientific, Německo) vybaveném elektrosprejovým ionizačním zdrojem. Bioinformatická analýza byla provedena pomocí Compound Discoverer Software (Thermo Fisher Scientific, USA), statistická analýza dat byla provedena v programovacím jazyce Python pomocí knihovny SciPy. Výsledky: Pomocí UHPLC-MS bylo v krevní plazmě identifikováno 1 049 metabolitů různých tříd. U pacientek s OC mělo 8 metabolitů významně nižší koncentraci (p < 0,01) ve srovnání se zdravými dárci, zatímco u 19 látek byly zjištěny vyšší hladiny (p < 0,01). Během metabolomického profilování vzorků moči bylo identifikováno 417 metabolitů: 12 látek mělo významně nižší koncentraci ve srovnání se zjevně zdravými jedinci a u 14 látek byly hladiny vyšší (p < 0,01). U pacientek se serózním adenokarcinomem vaječníků byla zjištěna významná změna v metabolomu krevní plazmy a moči, vyjádřená abnormálními koncentracemi lipidů a jejich derivátů, mastných kyselin a jejich derivátů, acylkarnitinů, fosfolipidů, aminokyselin a jejich derivátů, derivátů dusíkatých bází a steroidů. Mezi nejslibnější markery tohoto onemocnění přitom patří kynurenin, kyselina myristová, lysofosfatidylcholin a L-oktanoylkarnitin. Závěr: Odhalené změny v metabolomu se mohou stát základem pro zlepšení přístupů k diagnostice serózního ovariálního adenokarcinomu.
Background: The search for effective biomarkers for ovarian cancer (OC) early diagnosis is an urgent task of modern oncogynecology. Metabolic profiling by ultra-high performance liquid chromatography and mass spectrometry (UHPLC-MS) provides information on the totality of all low molecular weight metabolites of patient’s biological fluids sample, reflecting the processes occurring in the body. The aim of the study was to research blood plasma and urine metabolomic profile of patients with serous ovarian adenocarcinoma by UHPLC-MS. Material and methods: To perform metabolomic analysis, 60 blood plasma samples and 60 urine samples of patients diagnosed with serous ovarian carcinoma and 20 samples of apparently healthy volunteers were taken. Chromatographic separation was performed on a Vanquish Flex UHPLC System chromatograph (Thermo Scientific, Germany). Mass spectrometric analysis was performed on an Orbitrap Exploris 480 (Thermo Scientific, Germany) equipped with an electrospray ionization source. Bioinformatic analysis was performed using Compound Discoverer Software (Thermo Fisher Scientific, USA), statistical data analysis was performed in the Python programming language using the SciPy library. Results: Using UHPLC-MS, 1,049 metabolites of various classes were identified in blood plasma. In patients with OC, 8 metabolites had a significantly lower concentration (P < 0.01) compared with conditionally healthy donors, while the content of 19 compounds, on the contrary, increased (P < 0.01). During the metabolomic profiling of urine samples, 417 metabolites were identified: 12 compounds had a significantly lower concentration compared to apparently healthy individuals, the content of 14 compounds increased (P < 0.01). In patients with ovary serous adenocarcinoma, a significant change in the metabolome of blood plasma and urine was found, expressed in abnormal concentrations of lipids and their derivatives, fatty acids and their derivatives, acylcarnitines, phospholipids, amino acids and their derivatives, derivatives of nitrogenous bases and steroids. At the same time, kynurenine, myristic acid, lysophosphatidylcholine and L-octanoylcarnitine are the most promising markers of this disease. Conclusion: The revealed changes in the metabolome can become the basis for improving approaches to the diagnosis of serous ovarian adenocarcinoma.
Úvod: ischemická choroba srdeční (ichS) je celosvětově hlavní příčinou úmrtí, což je jedním z důvodů významných technologických pokroků intervenční kardiologie v posledních dekádách. Kombinace intravaskulárního ultrazvuku (iVuS) a infračervenému spektru blízké spektroskopie (nirS) nabízí detailní pohled na morfologii koronárních tepen a složení aterosklerotických plátů. tyto pokročilé zobrazovací technologie umožňují přesnější hodnocení ichS a zlepšují terapeutické strategie. Cíl: nirS dokáže detekovat a určitým způsobem kvantifikovat obsah lipidů v aterosklerotickém plátu pomocí indexů lcBi (lipid-core Burden index) a maxlcBi4mm (Maximal lipid core Burden index ve 4mm segmen- tu). V této studii jsme zkoumali vztah mezi těmito indexy a progresí aterosklerotického plátu v období 9–12 měsíců od perkutánní koronární intervence (Pci) s implantací lékového stentu v kmeni levé koronární tepny. Metody: Jednalo se o prospektivní, monocentrickou studii zahrnující 27 pacientů s významnou stenózou kmene levé věnčité tepny, kteří podstoupili iVuS-nrS vedenou Pci. Sériově byly hodnoceny lcBi, maxlcBi- 4mm a objem plátu (PV) před Pci, bezprostředně po Pci a v rámci kontrolního vyšetření za 9 až 12 měsíců u 18 pacientů. Výsledky: Průměrný věk pacientů dosahoval 72,7 roku, přičemž převládali muži (88 %). Průměrný lcBi před Pci byl 128,9 ± 122,0, zatímco maxlcBi4mm byl 263,7 ± 172,0. Volumetricky pomocí iVuS měřený PV po Pci byl 418,7 ± 203,3 mm3, při kontrole se zvýšil na 454,5 ± 209,4 mm3 (p = 0,105). Analýza neodhalila významnou korelaci mezi rozdílem PV (po Pci a při kontrole) a výchozími hodnotami lcBi, resp. maxlcBi4mm (p = 0,626, resp. 0,786). Závěr: Výsledky neprokázaly statisticky významnou korelaci mezi počátečními hodnotami lcBi, resp. maxlcBi4mm a progresí PV v kmeni levé věnčité tepny. Korelační grafy však naznačují trend ke snížení PV u pacientů s vysokými výchozími hodnotami lcBi a maxlcBi4mm jako možný efekt vysoce intenzivní statinové terapie.
Background: Coronary artery disease (CAD) is the leading cause of death worldwide, which has driven significant advances in the field of interventional cardiology. The combination of intravascular ultrasound (IVUS) and near-infrared light spectroscopy (NIRS) offers a detailed view of coronary artery morphology and plaque composition. This advanced imaging capability facilitates a more accurate assessment of CAD and enhances therapeutic strategies. Objective: NIRS can detect and quantify lipid content in atherosclerotic plaque using the Lipid Core Burden Index (LCBI) and the Maximal Lipid Core Burden Index in a 4 mm segment (maxLCBI4mm). In this study, we examined the relationship between these indices and atherosclerotic plaque progression in the crucial area of the left main coronary artery (LM) during a follow-up period of 9 to 12 months after percutaneous coronary interventions (PCI). Methods: A prospective, single-centre study was conducted involving 27 patients with significant left main stenosis who underwent IVUS-NIRS guided PCI. Serial assessments of the LCBI, maxLCBI4mm, and IVUS-derived plaque volume (PV) were performed at baseline, immediately post-PCI, and during follow-up at 9 to 12 months in 18 patients. Results: The mean age of the study population was 72.7 years, with a predominance of male patients (88%). The average LCBI of the LM coronary artery before PCI was 128.9 ± 122.0, while maxLCBI4mm was 263.7 ± 172.0. The IVUS-measured PV post-PCI was 418.7 ± 203.3 mm3, increasing to 454.5 ± 209.4 mm3 at follow-up (p = 0.105). Analysis revealed no significant correlation between the difference in PV and baseline LCBI or maxLCBI4mm, with p-values of 0.626 and 0.786, respectively. Conclusion: This study found no significant association between initial LCBI and maxLCBI4mm segment values and subsequent changes in PV in the left main coronary artery. However, correlation graphs indicate a trend toward decreased PV in patients with high initial LCBI and maxLCBI4mm.
- MeSH
- Plaque, Atherosclerotic * chemistry pathology MeSH
- Spectroscopy, Near-Infrared * methods instrumentation MeSH
- Ultrasonography, Interventional MeSH
- Percutaneous Coronary Intervention MeSH
- Coronary Stenosis surgery diagnostic imaging MeSH
- Humans MeSH
- Lipids analysis MeSH
- Prospective Studies MeSH
- Aged MeSH
- Statistics as Topic MeSH
- Health Status Indicators MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Clinical Study MeSH
- Research Support, Non-U.S. Gov't MeSH
PURPOSE: This prospective pilot study aims to evaluate the capabilities of novel quantitative ultrasound (QUS) methods based on attenuation (Att.PLUS) and sound speed (SSp.PLUS) for detecting liver fat. PATIENTS AND METHODS: The study included 56 individuals with biopsy-proven steatosis (percutaneous liver biopsy) ranging from 0 % to 90 % of hepatocytes containing intracellular lipid vacuoles. Histopathology was considered reference standard. Abdominal QUS examinations were conducted using Att.PLUS and SSp.PLUS techniques on the Aixplorer MACH 30 system. Comparative assessments were made using the results of liver biopsy and magnetic resonance spectroscopy (MRS) together with magnetic resonance imaging proton density fat fraction (MRI-PDFF). MR examinations were performed on the Siemens VIDA 3 T system. RESULTS: ROC analysis was conducted for two groups: (a) patients without steatosis (S0) versus those with steatosis (S1 + S2 + S3) yielded AUC values of 0.79 for Att.PLUS and 0.78 for SSp.PLUS, in contrast to an AUC > 0.95 for MRS and MRI-PDFF; and (b) patients without or with mild steatosis (S0 + S1) versus those with severe steatosis (S2 + S3), yielded AUC values of 0.93 for Att.PLUS and 0.89 for SSp.PLUS, in contrast to an AUC > 0.99 for MRS and MRI-PDFF. However, MR methods were superior in detecting liver fat content in obese patients and post-liver transplantation individuals. CONCLUSION: Both QUS parameters (Att.PLUS and SSp.PLUS) appear equivalent at differentiating S0 vs. (S1 + S2 + S3) patients, but the Att.PLUS parameter may be more effective at identifying advanced steatosis (S2 + S3). MR techniques outperformed QUS methods, making them more suitable for clinical studies.
- MeSH
- Biopsy MeSH
- Adult MeSH
- Liver diagnostic imaging pathology MeSH
- Middle Aged MeSH
- Humans MeSH
- Magnetic Resonance Spectroscopy methods MeSH
- Magnetic Resonance Imaging * methods MeSH
- Pilot Projects MeSH
- Prospective Studies MeSH
- Reproducibility of Results MeSH
- Aged MeSH
- Sensitivity and Specificity MeSH
- Ultrasonography * methods MeSH
- Fatty Liver * diagnostic imaging pathology MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
Chlorinated paraffins (CPs) are environmental pollutants extensively used in industries. While the use of short-chain chlorinated paraffins (SCCPs) has been restricted since 2017, the use of medium-chain chlorinated paraffins (MCCPs) has risen as their replacement. Due to lipophilic character, it can be expected that CPs enter the cells; however, the in vitro accumulation potential of CPs remains poorly understood. In this study, we aimed to explore the ability of SCCPs and MCCPs to accumulate in fat cells. We utilized an in vitro model of mouse 3T3-L1 preadipocytes and adipocytes. Using gas chromatography coupled with high-resolution mass spectrometry operated in negative chemical ionization mode, we determined the intracellular amounts of CPs. These compounds accumulated at rates of 8.5 ± 0.1 μg/gcells/h for SCCPs and 7.8 ± 0.3 μg/gcells/h for MCCPs when an initial concentration of 120 ng/ml was present in the medium. This rate increased approximately tenfold when the concentration of CPs was raised to 1200 ng/ml. CPs content in adipocytes steadily increased over 5 days, whereas preadipocytes accumulated 15-20 times less CPs. This highlights the importance of cellular lipid content, which was about 12 times higher in adipocytes. Furthermore, we found that the level of chlorine content in the CPs molecules significantly influenced their accumulation. Our results demonstrate that MCCPs exhibit a similar accumulation potential to SCCPs, with lipid content playing a crucial role. As with SCCPs, restrictions on the use of MCCPs in industry should be considered to mitigate their environmental and health impacts.
- MeSH
- 3T3-L1 Cells * MeSH
- Hydrocarbons, Chlorinated * metabolism toxicity MeSH
- Halogenation * MeSH
- Environmental Pollutants toxicity metabolism MeSH
- Lipid Metabolism drug effects MeSH
- Mice MeSH
- Paraffin * MeSH
- Gas Chromatography-Mass Spectrometry MeSH
- Adipocytes * metabolism drug effects MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Dry skin is a common condition that is experienced by many. Besides being particularly present during the cold season, various diseases exist all year round, leading to localized xerosis. To prevent it, the skin is provided with natural moisturizing factors (NMFs). They are small amino acids or derivatives found in the outermost layer of the skin, the stratum corneum (SC). They are often claimed to be highly efficient humectants, increasing the water content to maintain the fluidity of the skin. However, alternative mechanisms have been proposed, suggesting that NMFs themselves may act as lipid mobility amplifiers. This work aims at investigating the role of three NMFs, namely, urea (URE), glycerol (GLY), and urocanic acid/urocanate (UCA/UCO) in SC in silico models, considering two different levels of humidity. Molecular dynamic simulations showed an increase in the diffusion of different lipid components, mainly free fatty acids (FFAs) and ceramide acyl chain moieties, in the presence of either high water content or NMFs. The membrane properties were modified, as seen by an increased thickness and greater lateral stiffness. All NMFs exhibited a similar impact, whereas UCA revealed slight differences according to its charged state. By studying NMF-water intermolecular interactions, we highlighted the role of NMF as a regulator of membrane perturbations while ensuring membrane fluidity. This role allows NMFs to prevent destabilization of the skin membrane in the presence of high water content. This study, performed at an atomistic resolution, highlighted a strong H-bond network between lipids involving mainly ceramides but also all other components. This network can be modified in the presence of a high water concentration or NMFs, resulting in modifications of membrane properties, rationalizing hydration effects.
- MeSH
- Glycerol * chemistry MeSH
- Skin * chemistry metabolism MeSH
- Urocanic Acid chemistry metabolism MeSH
- Humans MeSH
- Urea * chemistry MeSH
- Molecular Dynamics Simulation * MeSH
- Water * chemistry MeSH
- Hydrogen Bonding * MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
The RNA content is crucial for the formation of nuclear compartments, such as nuclear speckles and nucleoli. Phosphatidylinositol 4,5-bisphosphate (PIP2) is found in nuclear speckles, nucleoli, and nuclear lipid islets and is involved in RNA polymerase I/II transcription. Intriguingly, the nuclear localization of PIP2 was also shown to be RNA-dependent. We therefore investigated whether PIP2 and RNA cooperate in the establishment of nuclear architecture. In this study, we unveiled the RNA-dependent PIP2-associated (RDPA) nuclear proteome in human cells by mass spectrometry. We found that intrinsically disordered regions (IDRs) with polybasic PIP2-binding K/R motifs are prevalent features of RDPA proteins. Moreover, these IDRs of RDPA proteins exhibit enrichment for phosphorylation, acetylation, and ubiquitination sites. Our results show for the first time that the RDPA protein Bromodomain-containing protein 4 (BRD4) associates with PIP2 in the RNA-dependent manner via electrostatic interactions, and that altered PIP2 levels affect the number of nuclear foci of BRD4 protein. Thus, we propose that PIP2 spatiotemporally orchestrates nuclear processes through association with RNA and RDPA proteins and affects their ability to form foci presumably via phase separation. This suggests the pivotal role of PIP2 in the establishment of a functional nuclear architecture competent for gene expression.
- MeSH
- Cell Nucleus * metabolism genetics MeSH
- Phosphatidylinositol 4,5-Diphosphate * metabolism MeSH
- Phosphorylation MeSH
- Nuclear Proteins * metabolism genetics MeSH
- Humans MeSH
- Cell Cycle Proteins metabolism genetics MeSH
- Bromodomain Containing Proteins MeSH
- RNA metabolism genetics MeSH
- Transcription Factors * metabolism genetics MeSH
- Protein Binding MeSH
- Intrinsically Disordered Proteins * metabolism genetics chemistry MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
