8 sv.
- MeSH
- Food Analysis MeSH
- Food Microbiology MeSH
- Publication type
- Periodical MeSH
- Conspectus
- Potravinářský průmysl
- NML Fields
- nutriční terapie, dietoterapie a výživa
- zemědělství a potravinářství
- technika
Juvenilná hyalínová fibromatóza je zriedkavé autozomálne recesívne dědičné ochorenie charakterizované prítomnosťou nodulov a tumorov v koži a mákkých tkanivách i gingiválnych hyperplázií. Autoři ho popísali u dospělého 28-ročného muža na základe histopatologickej diagnózy. Pacient bol počas života tri rázy hospitalizovaný. Diagnostikovaná artrogrypóza. Pri poslednej hospitalizaci!, kde bol pryatý pre sekundámu impetiginizáciu rozsiahlych ploch koze na končatinách a pachydermiu, bol zistený polymalformačný syndrom, mnohočetné tumory podkožia, gingiválna hypertofia, kontraktúry, osteolytické lézie a pozitivna rodinná anamnéza. Histologicky tumoriformné lézie obsahovali hyalínovú bezštruktúmu matrix často s chondroidnou až osteoidnou metapláziou, přítomné kalciové soli. V matrix holi zanořené viae či menej početné buňky fibroblastoidného vzhl'adu s eozinofílnou cytoplazmou, oválným jadrom a často pericytoplazmatickým haló. Elektrónmikroskopickým vyšetřením sa dokázali dilatovi )vané cisterny hrubého endoplazmatického retikula a hypertrofický Golgiho aparát. Ojediněle holi detekované partikuly denzitou zodpoved^úce kalciovým soliam. Imunohistochemicky tumorózne buňky exprimovali vimentin, alfa-1-antichymotiypsín a alfa-1-antitrypsín. Získané poznatky zodpovedsgu v písomníctve známým nálezom. Imunohistochemicky obraz sa v dostupnej literature neuvádza.
Juvenile hyaline fibromatosis is a rare autosomal recessive interstitial disease characterized by nodes and tumours of skin and soft tissues as well as by gingival hyperplasia. The authors described a case of 28-year-old male based on histopathological diagnosis. The patient was admitted to the hospital thrice in his life with the diagnosis of arthrogryphosis. Last time he presented with extensive secondary impetigo in extremities and pachydermia, polymalformation syndrom, multiple subcutaneous tumours, gingival hypertrophy, contractures, osteolytic lesions and positive family history. In histology, tumoriform lesions showed a structureless hyaline matrix often with chondroosseous metaplasia and calcium salts. More or less numerous cells in the matrix had a fíbroblastoid appearance with eosinophilic cytoplasm, oval nuclei and frequently pericytoplasmic halo. Electron microscopy revealed dilated cisterns of rough endoplasmic reticulum and a hypertrophied Golgi apparatus. Particles representing calcium salts according to their density were rare. Immunohistochemistry of tumour ceUs showed vimentin, alfa-l-antichymotrypsin and alfa-1-antitrypsin. The findings concurred with the literature in which, nevertheless, the immunohistochemical picture were not mentioned.
- MeSH
- Desmoid Tumors anatomy & histology pathology MeSH
- Adult MeSH
- Microscopy, Electron methods MeSH
- Genetic Diseases, Inborn MeSH
- Immunohistochemistry methods MeSH
- Humans MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Male MeSH
- Publication type
- Case Reports MeSH
- MeSH
- Cells pathology ultrastructure MeSH
- Chondrocytes cytology pathology ultrastructure MeSH
- Microscopy, Electron * methods utilization MeSH
- Homeostasis physiology immunology MeSH
- Cartilage, Articular * pathology ultrastructure injuries MeSH
- Knee Joint * pathology ultrastructure MeSH
- Humans MeSH
- Lysosomes pathology ultrastructure MeSH
- Necrosis etiology metabolism pathology MeSH
- Tissues pathology ultrastructure injuries MeSH
- Check Tag
- Humans MeSH
Patofyzioiogickým podkladem narkolepsie-kataplexie je ztráta hypokretinových neuronů posterolaterálního hypotalamu. Předpokládaným projevem tohoto deficitu je změna mikrostruktury a autonomních funkcí ve spánku u těchto pacientů. Cílem studie bylo hodnocení mikrostruktury NREM (non-rapid eye movement) spánku metodou sledování cyklických alternujících vzorců (CAP) a změny variability srdeční frekvence (HRV). Do studie bylo zahrnuto 15 pacientů s narkolepsiíkataplexií (průměrný věk 35 8,5 věkové rozmezí 22-44 let) a 15 zdravých kontrol (31 ± 11,4; 19-48 let). Obě skupiny podstoupily 2 následná polysomnografická vyšetření, pro analýzu CAP a HRV byla zpracována data ze 2. noci. Prokázali jsme signifikantní snížení CAP, provázené sníŽením LF a zvýšením HF složky při redukci poměru LF/HF v průběhu NREM spánku. Výsledky vyjadřují poruchu kolísání prahu probuzení u narkolepsie-kataplexie, která je provázena redukcí tonu sympatiku během NREM spánku. Domníváme se, narkolepsie nevzniká pouze v důsledku poruchy regulace REM spánku, nýbrž že je současně vlivem deficientní hypokretinové modulace alt porušena i regulace NREM spánku.
The present study was aimed at analysing the non-rapid eye movement (NREM) sleep microstructure by the cyclic alternating pattern (CAP) and at assessing the heart rate variability (HRV) changes in patiens with narcolepsy, hypothesizing a correlation of an abnormal sleep microstructure and abnormal autonomic response with a selective loss of hypocretin-containing neurons in narcolepsy. Fifteen patients with narcolepsy-cataplexy (mean age 35±8.5; age range 22-44), and 15 age and sex matched controls (mean age 31±11.4; age range 19-48) were included in the study. All subjects underwent polysomnography recordings for two consecutive nights in a standard laboratory setting. The sleep scoring was focused on the CAP and HRV analysis. A significant decrease in CAP rate as well as significant reduction of the LF spectral band and the LF/HF ratio, and the elevation of the HF spectral component during NREM 4 stage were revealed in narcoleptics compared to controls. Our results suggest that physiological fluctuation of arousal during sleep described as CAP is impaired in narcolepsy and accompained by reduced sympathetic tone during SWS. We have hypothesized that the whole sleep regulation process is altered in narcolepsy and not only REM sleep mechanisms.
- MeSH
- Electroencephalography methods utilization MeSH
- Research Support as Topic MeSH
- Data Interpretation, Statistical MeSH
- Humans MeSH
- Narcolepsy diagnosis etiology MeSH
- Neuropeptides deficiency MeSH
- Sleep Wake Disorders diagnosis etiology physiopathology MeSH
- Sleep, REM physiology MeSH
- Heart Rate physiology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Comparative Study MeSH
Taurine, a sulphur - containing amino acid, has been termed a functional nutrient. Its synthetic form is a common ingredient in supplements and energy drinks. There is no information concerning taurine impact on bone microstructure after prolonged supplemental use. Also, differences in bone parameters of mice following taurine exposure are unknown. In this study, a detailed microstructure of compact and trabecular bone tissues of mice subchronically exposed to taurine was determined. Animals (n=12) were segregated into three groups: E1 group - mice received 20 mg/kg b.w. of taurine per day during 8 weeks; E2 group - mice were fed by taurine at a dose of 40 mg/kg b.w. for 8 weeks and a control (C) group. Decreased density of secondary osteons, increased sizes of primary osteon's vascular canals (P<0.05) were observed in taurine - treated animals. Cortical bone thickness, trabecular thickness were decreased (P<0.05) in E1 group, and relative volume of trabecular bone was lower (P<0.05) in E2 group as compared to C group. According to our results, prolonged taurine exposure at the doses used in this study can negatively affect both compact and trabecular bone tissues microstructure.
- MeSH
- Femur drug effects pathology physiology MeSH
- Cortical Bone cytology drug effects physiology MeSH
- Bone Density drug effects physiology MeSH
- Mice MeSH
- Random Allocation MeSH
- Drug Administration Schedule MeSH
- Taurine administration & dosage toxicity MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Acrylamide (AA) is one of the most common toxins in foods. Its effect on bone microstructure has not been investigated. The aim of our study was to analyze the impact of acute exposure to AA on femoral bone microstructure in mice. Adult animals were treated perorally with 2 doses of AA (E1 group, 1 mg/kg b.w.) in a 24-h period and with 3 doses of AA (E2 group, 1 mg/kg b.w.) in a 48-h period. Mice exposed to AA had smaller sizes of primary osteon's vascular canals. Secondary osteons were significantly smaller in mice from E2 group; however their increased number (from 38 % to 77 %) was identified in both E1 and E2 groups. In these groups, a higher number of resorption lacunae (from 100 % to 122 %) was also found. The values for bone volume, trabecular number were increased and that for trabecular separation was decreased in mice administered AA. Significantly higher value of bone surface was observed in mice from E1 group whereas trabecular thickness was increased in E2 group. The effect of AA on microstructure of compact and trabecular bone tissues is different. In our study, one dose of AA was used and acute effects of AA were investigated. Therefore, further studies are needed to study mechanisms by which AA acts on bone.
- MeSH
- Acrylamide toxicity MeSH
- Femur diagnostic imaging drug effects pathology MeSH
- Food Contamination * MeSH
- Cortical Bone diagnostic imaging drug effects pathology MeSH
- Mice MeSH
- X-Ray Microtomography MeSH
- Cancellous Bone diagnostic imaging drug effects pathology MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
