... Buser -- 2 Proceedings of the Third III Consensus Conference: -- Implants in Post-Extraction Sites 3 ... ... Statements 6 -- 2.1.2 Proposed Clinical Approaches 7 -- 2.1.3 Conclusions 8 -- 2.2 Implants in Post-Extraction ... ... Update 11 -- 2.2.3 Healing Adjacent to Implants in Post-Extraction Sites 12 -- 2.2.4 Implant Survival ... ... 13 -- 2.2.5 Esthetic Outcomes I4 -- 3 Pre-Operative Assessment and Treatment Options for Post-Extraction ... ... Implants 17 -- 3.1 Factors Influencing the Treatment Outcomes of Implants in Post-Extraction Sites 18 ...
ITI treatment guide ; Vol. 3
xiii, 202 s. : il., tab. ; 29 cm
- MeSH
- Tooth Extraction MeSH
- Dental Implantation classification methods MeSH
- Oral Surgical Procedures MeSH
- Dental Implants, Single-Tooth MeSH
- Dental Implants MeSH
- Conspectus
- Stomatologie
- NML Fields
- zubní lékařství
- NML Publication type
- kolektivní monografie
The authors describe their own manual technique of extracapsular cataract extraction with subsequent implantation of a synthetic intraocular lens. They give an account of results assembled in 83 patients after ECE, incl. 60 where an intraocular lens was implanted. They achieved very satisfactory results with a minimal number of complications, one of which was more serious. In the conclusion the authors emphasize that final evaluation calls for a longer follow-up period.
- MeSH
- Adult MeSH
- Cataract Extraction methods MeSH
- Middle Aged MeSH
- Humans MeSH
- Lenses, Intraocular MeSH
- Postoperative Complications MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- MeSH
- Bleeding Time MeSH
- Tooth Extraction MeSH
- Hemostatics administration & dosage contraindications therapeutic use MeSH
- Hemostasis MeSH
- Publication type
- Review MeSH
Bylo provedeno srovnání devíti metod izolace specifické DNA etiologického agens humánní granulocytárníehrlichiózy z lidské krve pro účely vyšetření PCR. Do plné krve zdravého dárce byladefinovaně přimíšena laboratorní kultura agens a testována účinnost izolace a stabilita templátuza podmínek, kdy byla krev čerstvá nebo zmražená. K univerzálnímu použití byl nejvhodnějšíQIAamp® DNA Mini Kit (QIAGEN), zmražená krev byla nejlépe extrahována pomocí Nucleo SpinTissue (Macherey-Nagel).
The author compared nine methods for isolation of specific DNA of the etiological agent of humangranulocytic ehrlichiosis from human blood for examination of the PCR. To full blood of healthydonors a laboratory culture of the agent was added and the effectiveness of isolation an stability ofthe template was tested under conditions when the blood was fresh or frozen. For universal useQIAamp® (QIAGEN) was most suitable, frozen blood was extracted best using NucleoSpin® Tissue(Macherey-Nagel).
- MeSH
- Anaplasma pathogenicity MeSH
- DNA MeSH
- Ehrlichiosis diagnosis etiology MeSH
- Research Support as Topic MeSH
- Clinical Laboratory Techniques methods MeSH
- Humans MeSH
- Polymerase Chain Reaction MeSH
- Serial Extraction methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Review MeSH
- Comparative Study MeSH
Grape cane, leaves and grape marc are waste products from viticulture, which can be used to obtain secondary stilbene derivatives with high antioxidant value. The presented work compares several extraction methods: maceration at laboratory temperature, extraction at elevated temperature, fluidized-bed extraction, Soxhlet extraction, microwave-assisted extraction, and accelerated solvent extraction. To obtain trans-resveratrol, trans-ε-viniferin and r2-viniferin from grape cane of the V. vinifera variety Cabernet Moravia, various conditions were studied: different solvents, using powdered versus cut cane material, different extraction times, and one-step or multiple extractions. The largest concentrations found were 6030 ± 680 µg/g dry weight (d.w.) for trans-resveratrol, 2260 ± 90 µg/g d.w. for trans-ε-viniferin, and 510 ± 40 µg/g d.w. for r2-viniferin. The highest amounts of stilbenes (8500 ± 1100 µg/g d.w.) were obtained using accelerated solvent extraction in methanol.
- MeSH
- Time Factors MeSH
- Chemical Fractionation methods MeSH
- Canes * MeSH
- Nuclear Magnetic Resonance, Biomolecular MeSH
- Polyphenols chemistry isolation & purification MeSH
- Plant Extracts chemistry isolation & purification MeSH
- Solvents MeSH
- Stilbenes chemistry isolation & purification MeSH
- Temperature MeSH
- Vitis chemistry MeSH
- Chromatography, High Pressure Liquid MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Optimized acceptor solutions, which eliminate electrolytically induced variations in their pH values, have been shown to improve electromembrane extraction (EME) performance. Acceptor solutions containing 500 mM formic acid (pH 1.97) ensured stable EME process for three basic drugs extracted at 50 V across 1-ethyl-2-nitrobenzene and constant extraction recoveries (66-89%) were achieved for 40-80 min EMEs. Back-extraction of analytes into donor solutions has been eliminated by application of optimized acceptor solutions, moreover, saturation of acceptor solutions with analytes had no additional effect on their back-extraction; the presence of up to 300-fold excess of analytes in optimized acceptor solutions led to slightly reduced but stable enrichment of analytes over the entire extraction time. Stable EME performance has been also achieved for extractions into 100mM HCl, note however, that seriously compromised performance of subsequent capillary electrophoretic analyses has been observed due to high conductivities of resulting acceptor solutions. Electrolytically produced H(+) and OH(-) ions have mostly remained in corresponding operating solutions, have determined their final pH values and have not been subjects of EME transfers across selective phase interfaces as was experimentally verified by pH measurements of anolytes and catholytes at various EME times.
DNA extraction from soil samples is a critical step for molecular biology analyses. The present study compared the efficiency of two DNA isolation methods from non-polluted and polluted soils with or without the presence of a plant. Both applied methods used chemical and physical lyses, but method 1 had an additional physical disruption. The main difference between these two methods was the humic acid purification technique as it was carried out during cell lysis for method 1 and after cell lysis for method 2. Samples were assessed on the basis of their yield and DNA purity as well as their bacterial quantity and diversity. Based on our results, method 1 proved to be more effective at removing protein and RNA, whereas method 2 proved to be more effective at removing humic acids. Although no differences were obtained in terms of the DNA yield, both the bacterial quantity and community structure were affected by the method used. Method 1 allowed for the recovery of more information than method 2, and polluted soil was more sensitive to the DNA extraction procedure. We recommend carefully selecting the DNA extraction method, especially when soil is disturbed.
- MeSH
- Bacteria classification genetics isolation & purification metabolism MeSH
- Chemistry Techniques, Analytical methods MeSH
- DNA, Bacterial genetics isolation & purification MeSH
- Humic Substances MeSH
- Soil Pollutants analysis metabolism MeSH
- Polymerase Chain Reaction MeSH
- Soil chemistry MeSH
- Soil Microbiology MeSH
- Environmental Pollution MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
