foodborne pathogens Dotaz Zobrazit nápovědu
557 s. : il., tab. ; 26 cm
2nd revised and expanded ed. 677 s.
The ability of bacteria to bind different compounds and to adhere to biotic and abiotic surfaces provides them with a range of advantages, such as colonization of various tissues, internalization, avoidance of an immune response, and survival and persistence in the environment. A variety of bacterial surface structures are involved in this process and these promote bacterial adhesion in a more or less specific manner. In this review, we will focus on those surface adhesins and exopolymers in selected foodborne pathogens that are involved mainly in primary adhesion. Their role in biofilm development will also be considered when appropriate. Both the clinical impact and the implications for food safety of such adhesion will be discussed.
The intake of microbial-contaminated food poses severe health issues due to the outbreaks of stern food-borne diseases. Therefore, there is a need for precise detection and identification of pathogenic microbes and toxins in food to prevent these concerns. Thus, understanding the concept of biosensing has enabled researchers to develop nanobiosensors with different nanomaterials and composites to improve the sensitivity as well as the specificity of pathogen detection. The application of nanomaterials has enabled researchers to use advanced technologies in biosensors for the transfer of signals to enhance their efficiency and sensitivity. Nanomaterials like carbon nanotubes, magnetic and gold, dendrimers, graphene nanomaterials and quantum dots are predominantly used for developing biosensors with improved specificity and sensitivity of detection due to their exclusive chemical, magnetic, mechanical, optical and physical properties. All nanoparticles and new composites used in biosensors need to be classified and categorized for their enhanced performance, quick detection, and unobtrusive and effective use in foodborne analysis. Hence, this review intends to summarize the different sensing methods used in foodborne pathogen detection, their design, working principle and advances in sensing systems.
- MeSH
- Bacteria izolace a purifikace patogenita MeSH
- biosenzitivní techniky * MeSH
- grafit chemie MeSH
- lidé MeSH
- nanočástice chemie MeSH
- nanostruktury chemie MeSH
- nanotechnologie trendy MeSH
- nanotrubičky uhlíkové chemie MeSH
- nemoci přenášené potravou diagnóza mikrobiologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
2nd revised and expanded ed. 515 s.
- Konspekt
- Veřejné zdraví a hygiena
- NLK Obory
- environmentální vědy
- parazitologie
sv. : il.
- MeSH
- potravinářská mikrobiologie * MeSH
- potravinářská parazitologie MeSH
- Publikační typ
- periodika MeSH
- Konspekt
- Potravinářský průmysl
- NLK Obory
- zemědělství a potravinářství
Multiplex oligonucleotide ligation-PCR (MOL-PCR) is a rapid method for simultaneous detection of multiple molecular markers within a single reaction. MOL-PCR is increasingly employed in microbial detection assays, where its ability to facilitate identification and further characterization via simple analysis is of great benefit and significantly simplifies routine diagnostics. When adapted to microsphere suspension arrays on a MAGPIX reader, MOL-PCR has the potential to outperform standard nucleic acid-based diagnostic assays. This study represents the guideline towards in-house MOL-PCR assay optimization using the example of foodborne pathogens (bacteria and parasites) with an emphasis on the appropriate choice of crucial parameters. The optimized protocol focused on specific sequence detection utilizes the fluorescent reporter BODIPY-TMRX and self-coupled magnetic microspheres and allows for a smooth and brisk workflow which should serve as a guide for the development of MOL-PCR assays intended for pathogen detection.
- MeSH
- infekce yersiniemi diagnóza mikrobiologie MeSH
- lidé MeSH
- multiplexová polymerázová řetězová reakce metody MeSH
- nemoci přenášené potravou diagnóza mikrobiologie parazitologie MeSH
- Toxoplasma genetika izolace a purifikace MeSH
- toxoplazmóza diagnóza parazitologie MeSH
- Yersinia enterocolitica genetika izolace a purifikace MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Přes stále se zvyšující úroveň hygieny potravin v České republice i ostatních členských státech Evropské unie představují i na počátku 21. století původci alimentárních infekcí přenosných ze zvířat na člověka velký problém v humánní i veterinární medicíně. Příspěvek se věnuje problematice vybraných bakteriálních alimentárních zoonóz v ČR a státech EU, včetně antibiotické rezistence jejich původců. Jsou uvedeny některé dílčí výsledky prevalence původců alimentárních onemocnění v potravinovém řetězci člověka na území Moravy v letech 2007 a 2008.
Despite the increasing level of food hygiene in the Czech Republic and other European Union member states, agents causing diseases transmitted from animals to human remain a serious problem in both human and veterinary medicine of the 21st century. The article is concerned with selected bacterial foodborne zoonoses in the Czech Republic and EU member states, including antibiotic resistance of the pathogens. Some preliminary results on the prevalence of pathogens in the human food chain in the Czech region of Moravia in 2007-2008 are presented.
The fast and efficient detection of foodborne pathogens is a societal priority, given the large number of food-poisoning outbreaks, and a scientific and technological challenge, given the need to detect as little as 1 viable cell in 25 gr of food. Here, we present the first approach that achieves the above goal, thanks to the use of a micro/nano-technology and the detection capability of acoustic wave sensors. Starting from 1 Salmonella cell in 25 ml of milk, we employ immuno-magnetic beads to capture cells after only 3 h of pre-enrichment and subsequently demonstrate efficient DNA amplification using the Loop Mediated Isothermal Amplification method (LAMP) and acoustic detection in an integrated platform, within an additional ½ h. The demonstrated 4 h sample-to-analysis time comes as a huge improvement to the current need of few days to obtain the same result. In addition, the work presents the first reported Lab-on-Chip platform that comprises an acoustic device as the sensing element, exhibiting impressive analytical features, namely, an acoustic limit of detection of 2 cells/μl or 3 aM of the DNA target and ability to detect in a label-free manner dsDNA amplicons in impure samples. The use of food samples together with the incorporation of the necessary pre-enrichment step and ability for multiple analysis with an internal control, make the proposed methodology highly relevant to real-world applications. Moreover, the work suggests that acoustic wave devices can be used as an attractive alternative to electrochemical sensors in integrated platforms for applications in food safety and the point-of-care diagnostics.
- MeSH
- akustika přístrojové vybavení MeSH
- analýza potravin přístrojové vybavení MeSH
- biosenzitivní techniky přístrojové vybavení MeSH
- design vybavení MeSH
- DNA bakterií analýza genetika MeSH
- kontaminace potravin analýza MeSH
- laboratoř na čipu MeSH
- lidé MeSH
- limita detekce MeSH
- mléko mikrobiologie MeSH
- nemoci přenášené potravou mikrobiologie MeSH
- potravinářská mikrobiologie MeSH
- Salmonella genetika izolace a purifikace MeSH
- salmonelóza mikrobiologie MeSH
- zvířata MeSH
- zvuk MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
Recent outbreaks of foodborne illnesses have shown that foodborne bacterial pathogens present a significant threat to public health, resulting in an increased need for technologies capable of fast and reliable screening of food commodities. The optimal method of pathogen detection in foods should: (i) be rapid, specific, and sensitive; (ii) require minimum sample preparation; and (iii) be robust and cost-effective, thus enabling use in the field. Here we report the use of a SPR biosensor based on ultra-low fouling and functionalizable poly(carboxybetaine acrylamide) (pCBAA) brushes for the rapid and sensitive detection of bacterial pathogens in crude food samples utilizing a three-step detection assay. We studied both the surface resistance to fouling and the functional capabilities of these brushes with respect to each step of the assay, namely: (I) incubation of the sensor with crude food samples, resulting in the capture of bacteria by antibodies immobilized to the pCBAA coating, (II) binding of secondary biotinylated antibody (Ab2) to previously captured bacteria, and (III) binding of streptavidin-coated gold nanoparticles to the biotinylated Ab2 in order to enhance the sensor response. We also investigated the effects of the brush thickness on the biorecognition capabilities of the gold-grafted functionalized pCBAA coatings. We demonstrate that pCBAA-compared to standard low-fouling OEG-based alkanethiolate self-assemabled monolayers-exhibits superior surface resistance regarding both fouling from complex food samples as well as the non-specific binding of S-AuNPs. We further demonstrate that a SPR biosensor based on a pCBAA brush with a thickness as low as 20 nm was capable of detecting E. coli O157:H7 and Salmonella sp. in complex hamburger and cucumber samples with extraordinary sensitivity and specificity. The limits of detection for the two bacteria in cucumber and hamburger extracts were determined to be 57 CFU/mL and 17 CFU/mL for E. coli and 7.4 × 10(3) CFU/mL and 11.7 × 10(3)CFU/mL for Salmonella sp., respectively. In addition, we demonstrate the simultaneous detection of E. coli and Salmonella sp. in hamburger sample using a multichannel SPR biosensor having appropriate functional coatings.
- MeSH
- biosenzitivní techniky * MeSH
- Escherichia coli O157 izolace a purifikace patogenita MeSH
- imobilizační protilátky chemie MeSH
- kontaminace potravin MeSH
- lidé MeSH
- limita detekce MeSH
- nemoci přenášené potravou diagnóza mikrobiologie MeSH
- potravinářská mikrobiologie * MeSH
- povrchová plasmonová rezonance MeSH
- zlato chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
