microencapsulation
Dotaz
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elektronický časopis
- Konspekt
- Farmacie. Farmakologie
- NLK Obory
- farmacie a farmakologie
- NLK Publikační typ
- elektronické časopisy
Nowadays, phage therapy emerges as one of the alternative solutions to the problems arising from antibiotic resistance in pathogenic bacteria. Although phage therapy has been successfully applied both in vitro and in vivo, one of the biggest concerns in this regard is the stability of phages in body environment. Within the scope of this study, microencapsulation technology was used to increase the resistance of phages to physiological conditions, and the resulting microcapsules were tested in environments simulating body conditions. For this purpose, Bacillus subtilis, Salmonella enterica subsp. enterica serovar Enteritidis (Salmonella Enteritidis), and Salmonella enterica subsp. enterica serovar Typhimurium (Salmonella Typhimurium) phages were isolated from different sources and then microencapsulated with 1.33% (w/v) sodium alginate using a spray dryer to minimize the damage of physiological environment. Stability of microcapsules in simulated gastric fluid and bile salt presence was tested. As a consequence, the maximum titer decrease of microencapsulated phages after 2-h incubation was found to be 2.29 log unit for B. subtilis phages, 1.71 log unit for S. Enteritidis phages, and 0.60 log unit for S. Typhimurium phages, while free phages lost their viability even after a 15-min incubation. Similarly, microencapsulation was found to increase the stability of phages in the bile salt medium and it was seen that after 3 h of incubation, the difference between the titers of microencapsulated phages and free phages could reach up to 3 log unit.
215 s. : il.
The purpose of this study was to improve the survival of Bifidobacterium animalis subsp. lactis 10140 during freeze-drying process by microencapsulation, using a special pediatric prebiotics mixture (galactooligosaccharides and fructooligosaccharides). Probiotic microorganisms were encapsulated with a coat combination of prebiotics-calcium-alginate prior to freeze-drying. Both encapsulated and free cells were then freeze-dried in their optimized combinations of skim milk and prebiotics. Response surface methodology (RSM) was used to produce a coating combination as well as drying medium with the highest cell viability during freeze-drying. The optimum encapsulation composition was found to be 2.1 % Na-alginate, 2.9 % prebiotic, and 21.7 % glycerol. Maximum survival predicted by the model was 81.2 %. No significant (p > 0.05) difference between the predicted and experimental values verified the adequacy of final reduced models. The protection ability of encapsulation was then examined over 120 days of storage at 4 and 25 °C and exposure to a sequential model of infantile GIT conditions including both gastric conditions (pH 3.0 and 4.0, 90 min, 37 °C) and intestinal conditions (pH 7.5, 5 h, 37 °C). Significantly improved cell viability showed that microencapsulation of B. lactis 10140 with the prebiotics was successful in producing a stable symbiotic powdery nutraceutical.
- MeSH
- Bifidobacterium fyziologie účinky záření MeSH
- časové faktory MeSH
- gastrointestinální trakt mikrobiologie MeSH
- kojenec MeSH
- lidé MeSH
- lyofilizace * MeSH
- mikrobiální viabilita účinky záření MeSH
- náhražky mateřského mléka MeSH
- počet mikrobiálních kolonií MeSH
- příprava léků MeSH
- probiotika účinky záření MeSH
- skladování léků metody MeSH
- teplota MeSH
- Check Tag
- kojenec MeSH
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Probiotic bacteria are widely used in pharmaceutics to offer health benefits. Microencapsulation is used to deliver probiotics into the human body. Capsules in the stomach have to keep bacteria constrained until release occurs in the intestine. Once outside, bacteria must maintain enough motility to reach the intestine walls. Here, we develop a platform based on two label-free optical modules for rapidly screening and ranking probiotic candidates in the laboratory. Bio-speckle dynamics assay tests the microencapsulation effectiveness by simulating the gastrointestinal transit. Then, a digital holographic microscope 3D-tracks their motility profiles at a single element level to rank the strains.
- Publikační typ
- časopisecké články MeSH
V posledních letech se lidé stále více zajímají o potraviny, které nabízejí vyváženou stravu a podporují lidské zdraví. To je důvod, proč poptávka po funkčních potravinách neustále roste. Mnoho studií se zaměřuje na stadium příznivého vlivu probiotik, které jsou jednou z hlavních součástí funkčních potravin, jakož i na zlepšení schopnosti probiotik přežít během průchodu trávicím traktem poskytování v životaschopné podobě až do tlustého střeva. Proto koncepce efektivního, nízkonákladového systému pro transport probiotických bakterií přitahuje stále větší pozornost odborníků. V tomto článku je testována mikroenkapsulace do alginátu sodného s cílem zlepšit životaschopnost Lb plantarum G1 v in vitro simulovaném žaludečním a střevním systému. Inkubace probíhala v přítomnosti tří nápojů, které jsou hojně konzumované v alžírské populaci a na celém světě – černá káva, zelený čaj a pomerančový džus. Cílem bylo přispět doporu- čením pro řádnou konzumaci probiotik. Výsledky ukázaly, že požití probiotika současně s jedním ze studovaných nápojů vedlo ke snížení životaschopnosti probiotických bakterií. Mikroenkapsulace do alginátu ovšem zlepšila jejich životaschopnost, dokonce i na konci gastrointestinálního systému. Viabilita bakterií v černé kávě, zeleném čaji a pomerančovém džusu byla 9.47, 9.07 a 10.39 log CFU/mL, resp.
In recent years, people are increasingly interested in foods which offer a balanced diet and promote human health. This is why the demand on functional foods is growing steadily. Many recent studies are focusing on the beneficial effect of probiotics, which are one of the main components of functional foods as well as improving their capacity to survive during passage through digestive tract to arrive viable and in sufficient amounts to colon. Therefore, the conception of an efficient, low-cost system for the delivery of probiotic bacteria is highly attracting food science researchers. In the current paper, microencapsulation within sodium alginate is tested for its capacity to improve Lb. plantarum G1 viability in an in vitro simulated gastrointestinal system designed in the laboratory. Incubation was realized in presence of three beverages that are commonly and highly consumed by Algerian population and worldwide they include black coffee, green tea and orange juice, the objective was to contribute in developing recommendations to properly consume probiotics, The results indicated that introducing probiotics simultaneously with one of the studied beverages resulted in a decrease in probiotic viability, however, microencapsulation within alginate beads improved its viability even at the end of the gastrointestinal simulated transit, suggesting that probiotics, when encapsulated, can reach the lower intestine. The cell viable counts for black coffee, green tea and orange juice were 9.47, 9.07 and 10.39 log CFU/mL, respectively.
- MeSH
- algináty chemie metabolismus MeSH
- gastrointestinální trakt chemie metabolismus MeSH
- Lactobacillus plantarum * metabolismus růst a vývoj účinky léků MeSH
- léky s prodlouženým účinkem chemie metabolismus MeSH
- potravní doplňky mikrobiologie MeSH
- probiotika aplikace a dávkování farmakokinetika MeSH
- techniky in vitro * metody přístrojové vybavení MeSH
- tobolky * chemie metabolismus MeSH
The aim of this study was optimization of spray-drying process conditions for microencapsulation of Turkish oregano extract. Different concentrations of maltodextrin and gum arabic as encapsulating agents (wall material) as well as influence of selected processing variables were evaluated. The optimal conditions were maintained on the basis of the load of main bioactive compounds - ursolic, rosmarinic acids and carvacrol - in prepared microparticles after comparison of all significant response variables using desirability function. Physicomechanical properties of powders such as flowability, wettability, solubility, moisture content as well as product yield, encapsulation efficiency (EE), density, morphology and size distribution of prepared microparticles have been determined. The results demonstrated that the optimal conditions for spray-drying mixture consisted of two parts of wall material solution and one part of ethanolic oregano extract when the feed flow rate was 40 mL/min and air inlet temperature -170 °C. Optimal concentration of wall materials in solution was 20% while the ratio of maltodextrin and gum arabic was 8.74:1.26.
- MeSH
- arabská guma MeSH
- dobromysl (rod) * MeSH
- příprava léků * MeSH
- tobolky MeSH
- vysoušení MeSH
- Publikační typ
- časopisecké články MeSH
