Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
1 svazek : ilustrace, tabulky ; 30 cm
Materials coming into contact with foodstuffs can transfer some components into the food in quantities hazardous for human health. Compounds contained in wrapping material migrate into food and along with consumed food they are pass to the digestive tract. The aim of the project is to identify health risks caused by consuming contaminated food by humans as well as to eliminate the risks and thus increase the consumers health protection. This project will take advantage of the use of the currently most advanced chromatography and mass spectrometry (Orbitrap) methods. Alternative toxicological methods in vitro on the basis of cell lines and tissue and organ models of human origin will be used to determine the biological impact of chemical compounds and mixtures migrating from packaging materials. Human population exposure and health hazards will be estimated.
Materiály, které přicházejí do styku s potravinami, mohou přenášet své složky do potravin v množstvích, která mohou být nebezpečná pro lidské zdraví. Látky přítomné v obalech migrují do potravin a s konzumovanými potravinami přecházejí do trávicího traktu. Cílem projektu je identifikovat zdravotní rizika z takto kontaminovaných potravin, eliminovat je a tím zvýšit ochranu zdraví spotřebitelů. Navrhovaný projekt bude využívat moderní chromatografické analytické metody ve spojení s hmotnostní spektroskopií pro identifikaci xenobiotik. Toxikologickými metodami in vitro na bázi buněčných, tkáňových a orgánových modelů lidských tkání budou zjišťovány biologické účinky chemických látek a směsí migrujících z materiálu obalů. Následně bude proveden odhad expozice a vyhodnoceno zdravotní riziko.
- MeSH
- balení výrobků MeSH
- bezpečnost potravin MeSH
- biotest MeSH
- hodnocení rizik MeSH
- kontaminace potravin MeSH
- obaly potravin MeSH
- ochrana spotřebitele MeSH
- papír MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- tandemová hmotnostní spektrometrie MeSH
- toxikologie MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- xenobiotika analýza MeSH
- zásobování potravinami MeSH
- Konspekt
- Potravinářský průmysl
- NLK Obory
- zemědělství a potravinářství
- toxikologie
- chemie, klinická chemie
- hygiena
- NLK Publikační typ
- závěrečné zprávy o řešení grantu IGA MZ ČR
Previous studies have shown that a large number of polyvinylchloride (PVC) lid gaskets exceed the existing migration limits for epoxidised soybean oil (ESBO) and correct prediction of ESBO release into food therefore appears to be a difficult issue. ESBO migration from PVC gaskets of metal closures into food simulants and food products from the Czech market is evaluated during a survey in 2009 and subsequently one in 2012 to assess progress in lid manufacturing and official testing conditions. ESBO migration from lids into various food simulants was studied at various temperatures (25, 40 and 60°C) during storage times up to 12 months. ESBO released into food simulants or food products was transmethylated, derivatised and analysed by GC-MS. The levels of ESBO migration in foodstuffs in 2012 exceeded the specific migration limit (SML) in fewer products in comparison with the previous survey. However, most of the products were analysed at a time far from the expiry date and exceedance of the SML at the end of the product shelf life is not therefore excluded. More severe test conditions (60°C for 10 days) for specific migration given by the current European Union legislation (Regulation (EU) No. 10/2011) still seem to be insufficient for the simulation of ESBO migration during long-term storage.
- MeSH
- analýza potravin MeSH
- difuze MeSH
- epoxidové sloučeniny analýza MeSH
- kontaminace potravin analýza MeSH
- obaly potravin * MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- polyvinylchlorid analýza MeSH
- sójový olej analýza MeSH
- teplota MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
Epoxidised soybean oil (ESBO) is widely used as a plasticiser and stabiliser mainly in food contact materials on the base of polyvinylchloride (PVC), especially in the gaskets of jar lids. PVC gaskets containing 10-37% of ESBO were prepared by the baking of PVC plastisols at various process temperatures (180-240°C) in the laboratory. ESBO migration into olive oil and 3% acetic acid was studied at various temperatures (4°C, 25°C, 40°C and 60°C) during a storage time up to 12 months. ESBO released into food simulants was transmethylated, derivatised and analysed by gas chromatography-mass spectrometry (GC/MS). The effect of food processing, i.e. pasteurisation (80°C and 100°C) and sterilisation (125°C) on ESBO migration was also evaluated. The results were critically assessed with respect to the test conditions of specific migration in accordance with the current European Union legislation (Regulation (EU) No. 10/2011). The levels of ESBO migration found confirmed that the test conditions (i.e. 40°C or 60°C, 10 days) representing contact in the worst foreseeable use scenario seem to be insufficient for the simulation of ESBO migration during long-term storage and thus do not provide satisfactory objective results.
- MeSH
- časové faktory MeSH
- chemické jevy MeSH
- epoxidové sloučeniny chemie MeSH
- kyselina octová MeSH
- obaly potravin přístrojové vybavení MeSH
- oleje rostlin MeSH
- polyvinylchlorid chemie MeSH
- sójový olej chemie MeSH
- teplota MeSH
- vysoká teplota MeSH
- zvláčňovadla analýza chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Residues of printing ink components were determined in 94 samples of packaging materials commercially used in the Czech Republic for food packaging. The samples tested included printed polyethylene and polypropylene films, co-extruded and laminated films, paperboard beverages boxes, foils for thermo sealing of polystyrene cups, and polypropylene cups. Printing ink components were extracted with diethylether, then separated and determined using gas chromatography-mass spectrometry (GC/MS). Fifty compounds potentially originating from printing were isolated, identified and quantified. No acute health risk for consumers were identified, even though several findings of high levels of photo-initiators (e.g. in polyethylene terephthalate (PET) films for thermo sealing of polystyrene cups) as well as plasticizers (acetyl tributyl citrate, tributyl aconitate, 2-butoxyethyl oleate and 2-ethylhexyl diphenyl phosphate in co-extruded films) indicate that their real migration into food and/or food simulants needs to be undertaken for a proper safety evaluation.
- MeSH
- fotochemické procesy MeSH
- hodnocení rizik MeSH
- inkoust MeSH
- kontaminace potravin analýza MeSH
- lidé MeSH
- obaly potravin MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- polymery analýza škodlivé účinky MeSH
- rozpouštědla analýza škodlivé účinky MeSH
- zvláčňovadla analýza škodlivé účinky MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- MeSH
- autoimunitní nemoci MeSH
- epidermolysis bullosa etiologie klasifikace MeSH
- kadheriny MeSH
- keratinocyty MeSH
- kůže anatomie a histologie patofyziologie MeSH
- lidé MeSH
- pemfigus etiologie MeSH
- vezikulobulózní nemoci kůže etiologie genetika patologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
