- MeSH
- cévní mozková příhoda * komplikace MeSH
- diferenciální diagnóza MeSH
- hemiplegie etiologie MeSH
- kineziologie aplikovaná metody MeSH
- lidé MeSH
- lopatka fyziologie MeSH
- nestabilita kloubu etiologie rehabilitace MeSH
- pohybové poruchy diagnóza etiologie MeSH
- polohování pacienta metody MeSH
- posturální rovnováha fyziologie MeSH
- ramenní kloub patofyziologie MeSH
- syndrom zhmožděného ramene * diagnóza etiologie prevence a kontrola MeSH
- týmová péče o pacienty MeSH
- Check Tag
- lidé MeSH
První vydání 83 stran ; 15 cm
Slovník, který se zaměřuje na zdravotnickou terminologii v češtině, slovenštině, polštině a ukrajinštině. Určeno odborné veřejnosti.
- Klíčová slova
- čeština, slovenština, polština, ukrajinština,
- MeSH
- lékařství MeSH
- terminologie jako téma MeSH
- Publikační typ
- slovník lékařský MeSH
- slovník vícejazyčný MeSH
- terminologické slovníky MeSH
- Konspekt
- Lékařské vědy. Lékařství
- Lingvistika. Jazyky
- NLK Obory
- lingvistika, lékařská terminologie
1. vydání 422 stran : ilustrace, tabulky ; 24 cm
Publikace, která se zaměřuje na postižení funkcí ruky, získané či vrozené, a na fyzioterapii ruky. Určeno odborné veřejnosti.
- MeSH
- deformity ruky diagnóza rehabilitace MeSH
- muskuloskeletální manipulace MeSH
- neurorehabilitace MeSH
- poranění ruky diagnóza terapie MeSH
- terapie cvičením MeSH
- Konspekt
- Fyzioterapie. Psychoterapie. Alternativní lékařství
- NLK Obory
- rehabilitační a fyzikální medicína
- NLK Publikační typ
- kolektivní monografie
Information on how insulin and insulin-like growth factors 1 and 2 (IGF-1 and -2) activate insulin receptors (IR-A and -B) and the IGF-1 receptor (IGF-1R) is crucial for understanding the difference in the biological activities of these peptide hormones. Cryo-EM studies have revealed that insulin uses its binding sites 1 and 2 to interact with IR-A and have identified several critical residues in binding site 2. However, mutagenesis studies suggest that Ile-A10, Ser-A12, Leu-A13, and Glu-A17 also belong to insulin's site 2. Here, to resolve this discrepancy, we mutated these insulin residues and the equivalent residues in IGFs. Our findings revealed that equivalent mutations in the hormones can result in differential biological effects and that these effects can be receptor-specific. We noted that the insulin positions A10 and A17 are important for its binding to IR-A and IR-B and IGF-1R and that A13 is important only for IR-A and IR-B binding. The IGF-1/IGF-2 positions 51/50 and 54/53 did not appear to play critical roles in receptor binding, but mutations at IGF-1 position 58 and IGF-2 position 57 affected the binding. We propose that IGF-1 Glu-58 interacts with IGF-1R Arg-704 and belongs to IGF-1 site 1, a finding supported by the NMR structure of the less active Asp-58-IGF-1 variant. Computational analyses indicated that the aforementioned mutations can affect internal insulin dynamics and inhibit adoption of a receptor-bound conformation, important for binding to receptor site 1. We provide a molecular model and alternative hypotheses for how the mutated insulin residues affect activity.
- MeSH
- insulinu podobný růstový faktor I chemie genetika MeSH
- insulinu podobný růstový faktor II chemie genetika MeSH
- inzulin analogy a deriváty chemická syntéza chemie genetika MeSH
- lidé MeSH
- mnohočetné abnormality genetika MeSH
- mutace genetika MeSH
- nukleární magnetická rezonance biomolekulární MeSH
- poruchy růstu genetika MeSH
- proteinové domény genetika MeSH
- receptor IGF typ 1 chemie genetika MeSH
- receptor inzulinu chemie genetika MeSH
- sekvence aminokyselin genetika MeSH
- vazba proteinů genetika MeSH
- vazebná místa genetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Acne vulgaris je jedno z nejčastějších kožních onemocnění mladé popluace. Nezanedbatelný je také psychosociální dopad choroby. Základem léčby je lokální terapie, vážné a rezistentní formy akné jsou indikovány k léčbě systémové. V přehledu jsou uvedeny též možnosti korektivní dermatologie.
Acne vulgaris is a very common skin problem in young population. Its social impact is very seriousThe pillar of acne treatment istopical therapy, patients with severe forms of acne are indicated for the use of systemic treatment. Some methods of correctivedermatology are presented.
- Klíčová slova
- kyselina azelaová,
- MeSH
- acne vulgaris * etiologie farmakoterapie patofyziologie MeSH
- antibakteriální látky škodlivé účinky terapeutické užití MeSH
- aplikace kožní MeSH
- benzoylperoxid terapeutické užití MeSH
- chemická exfoliace MeSH
- kosmetické techniky MeSH
- kyseliny dikarboxylové škodlivé účinky terapeutické užití MeSH
- lidé MeSH
- péče o kůži * MeSH
- retinoidy škodlivé účinky terapeutické užití MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
Insulin and insulin-like growth factor 1 (IGF-1) are closely related hormones involved in the regulation of metabolism and growth. They elicit their functions through activation of tyrosine kinase-type receptors: insulin receptors (IR-A and IR-B) and IGF-1 receptor (IGF-1R). Despite similarity in primary and three-dimensional structures, insulin and IGF-1 bind the noncognate receptor with substantially reduced affinity. We prepared [d-HisB24, GlyB31, TyrB32]-insulin, which binds all three receptors with high affinity (251 or 338% binding affinity to IR-A respectively to IR-B relative to insulin and 12.4% binding affinity to IGF-1R relative to IGF-1). We prepared other modified insulins with the aim of explaining the versatility of [d-HisB24, GlyB31, TyrB32]-insulin. Through structural, activity, and kinetic studies of these insulin analogs, we concluded that the ability of [d-HisB24, GlyB31, TyrB32]-insulin to stimulate all three receptors is provided by structural changes caused by a reversed chirality at the B24 combined with the extension of the C terminus of the B chain by two extra residues. We assume that the structural changes allow the directing of the B chain C terminus to some extra interactions with the receptors. These unusual interactions lead to a decrease of dissociation rate from the IR and conversely enable easier association with IGF-1R. All of the structural changes were made at the hormones' Site 1, which is thought to interact with the Site 1 of the receptors. The results of the study suggest that merely modifications of Site 1 of the hormone are sufficient to change the receptor specificity of insulin.
- MeSH
- insulinu podobný růstový faktor I chemie genetika metabolismus MeSH
- inzulin agonisté metabolismus MeSH
- kinetika MeSH
- krystalografie rentgenová MeSH
- lidé MeSH
- receptor inzulinu chemie genetika metabolismus MeSH
- receptory somatomedinů chemie genetika metabolismus MeSH
- sekvence aminokyselin MeSH
- vazba proteinů MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Insulin-like growth factors 1 and 2 (IGF-1 and -2, respectively) are protein hormones involved not only in normal growth and development but also in life span regulation and cancer. They exert their functions mainly through the IGF-1R or by binding to isoform A of the insulin receptor (IR-A). The development of IGF-1 and IGF-2 antagonists is of great clinical interest. Mutations of A4 and A8 sites of human insulin lead to disproportionate effects on hormone IR binding and activation. Here, we systematically modified IGF-1 sites 45, 46, and 49 and IGF-2 sites 45 and 48, which correspond, or are close, to insulin sites A4 and A8. The IGF-1R and IR-A binding and autophosphorylation potencies of these analogues were characterized. They retained the main IGF-1R-related properties, but the hormones with His49 in IGF-1 and His48 in IGF-2 showed significantly higher affinities for IR-A and for IR-B, being the strongest IGF-1- and IGF-2-like binders of these receptors ever reported. All analogues activated IR-A and IGF-1R without major discrepancies in their binding affinities. This study revealed that IR-A and IGF-1R contain specific sites, likely parts of their so-called sites 2', which can interact differently with specifically modified IGF analogues. Moreover, a clear importance of IGF-2 site 44 for effective hormone folding was also observed. These findings may facilitate novel and rational engineering of new hormone analogues for IR-A and IGF-1R studies and for potential medical applications.
- MeSH
- fosforylace MeSH
- insulinu podobný růstový faktor I chemie genetika MeSH
- insulinu podobný růstový faktor II chemie genetika MeSH
- inzulin chemie metabolismus MeSH
- lidé MeSH
- ligandy MeSH
- molekulární evoluce MeSH
- mutace MeSH
- protein - isoformy MeSH
- receptor inzulinu chemie metabolismus MeSH
- receptory somatomedinů chemie genetika MeSH
- signální transdukce MeSH
- vazba proteinů MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Human insulin-like growth factor 1 (IGF-1) is a 70 amino acid protein hormone, with key impact on growth, development, and lifespan. The physiological and clinical importance of IGF-1 prompted challenging chemical and biological trials toward the development of its analogs as molecular tools for the IGF-1 receptor (IGF1-R) studies and as new therapeutics. Here, we report a new method for the total chemical synthesis of IGF-1 analogs, which entails the solid-phase synthesis of two IGF-1 precursor chains that is followed by the CuI-catalyzed azide-alkyne cycloaddition ligation and by biomimetic formation of a native pattern of disulfides. The connection of the two IGF-1 precursor chains by the triazole-containing moieties, and variation of its neighboring sequences (Arg36 and Arg37), was tolerated in IGF-1R binding and its activation. These new synthetic IGF-1 analogs are unique examples of disulfide bonds' rich proteins with intra main-chain triazole links. The methodology reported here also presents a convenient synthetic platform for the design and production of new analogs of this important human hormone with non-standard protein modifications.
- MeSH
- arginin chemie MeSH
- buňky NIH 3T3 účinky léků MeSH
- cykloadiční reakce MeSH
- disulfidy chemie MeSH
- fibroblasty MeSH
- fosforylace MeSH
- insulinu podobný růstový faktor I analogy a deriváty chemická syntéza chemie metabolismus farmakologie MeSH
- lidé MeSH
- měď chemie MeSH
- methionin chemie MeSH
- myši MeSH
- preklinické hodnocení léčiv metody MeSH
- proteinové domény MeSH
- protoonkogenní proteiny c-akt metabolismus MeSH
- receptor IGF typ 1 metabolismus MeSH
- syntetická chemie okamžité shody MeSH
- techniky syntézy na pevné fázi MeSH
- triazoly chemie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
BACKGROUND: Dizziness is the second most frequent symptom that make patients seek specialized examination. The effective solution of dizzy conditions requires treatment in cooperation with different branches of medicine. OBJECTIVE: To analyze data from the database of patients with vertigo examined in the Hearing and Balance Disorder Centre in Ostrava, to find out whether, and in what factors, the sets of patients with diagnosed central and peripheral vestibular syndrom differ from each other. METHODS: Retrospective study that was carried out from October 2012 to February 2013. The data was gathered from documentation of all vertiginous patients who were examined by an otoneurologist. RESULTS: The statistically significant difference between the two sets was found in: occurrence of hypertension and mild obesity, impaired hearing and otitis media, stabilometric testing CONCLUSIONS: There was a statistically significant difference between the sets with the central and peripheral vestibular syndrome in the frequency of occurrence of hypertension, impaired hearing, otitis media, in mild obesity categorization and in balance disorders. There was not any statistically significant difference found in the other observed factors. The results confirm the need of a multidisciplinary approach to patients with vertigo.
- MeSH
- hypertenze epidemiologie MeSH
- komorbidita MeSH
- lidé MeSH
- migréna epidemiologie MeSH
- náhlá nedoslýchavost epidemiologie MeSH
- obezita epidemiologie MeSH
- otitis media epidemiologie MeSH
- retrospektivní studie MeSH
- stupeň závažnosti nemoci * MeSH
- vertigo epidemiologie MeSH
- závrať epidemiologie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika epidemiologie MeSH
Acne vulgaris je jedno z nejčastějších kožních onemocnění mladé popluace. Nezanedbatelný je také psychosociální dopad choroby. Základem léčby je lokální terapie, vážné a rezistentní formy akné jsou indikovány k léčbě systémové. V přehledu jsou uvedeny též možnosti korektivní dermatologie.
Acne vulgaris is a very common skin problem in young population. Its social impact is very seriousThe pillar of acne treatment istopical therapy, patients with severe forms of acne are indicated for the use of systemic treatment. Some methods of correctivedermatology are presented.
- Klíčová slova
- kyselina azelaová,
- MeSH
- acne vulgaris * etiologie farmakoterapie patofyziologie MeSH
- antibakteriální látky škodlivé účinky terapeutické užití MeSH
- aplikace kožní MeSH
- benzoylperoxid terapeutické užití MeSH
- chemická exfoliace MeSH
- kosmetické techniky MeSH
- kyseliny dikarboxylové škodlivé účinky terapeutické užití MeSH
- lidé MeSH
- péče o kůži * MeSH
- retinoidy škodlivé účinky terapeutické užití MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH