STUDY QUESTION: How has the interface between genetics and assisted reproduction technology (ART) evolved since 2005? SUMMARY ANSWER: The interface between ART and genetics has become more entwined as we increase our understanding about the genetics of infertility and we are able to perform more comprehensive genetic testing. WHAT IS KNOWN ALREADY: In March 2005, a group of experts from the European Society of Human Genetics and European Society of Human Reproduction and Embryology met to discuss the interface between genetics and ART and published an extended background paper, recommendations and two Editorials. STUDY DESIGN, SIZE, DURATION: An interdisciplinary workshop was held, involving representatives of both professional societies and experts from the European Union Eurogentest2 Coordination Action Project. PARTICIPANTS/MATERIALS, SETTING, METHODS: In March 2012, a group of experts from the European Society of Human Genetics, the European Society of Human Reproduction and Embryology and the EuroGentest2 Coordination Action Project met to discuss developments at the interface between clinical genetics and ART. MAIN RESULTS AND THE ROLE OF CHANCE: As more genetic causes of reproductive failure are now recognized and an increasing number of patients undergo testing of their genome prior to conception, either in regular health care or in the context of direct-to-consumer testing, the need for genetic counselling and PGD may increase. Preimplantation genetic screening (PGS) thus far does not have evidence from RCTs to substantiate that the technique is both effective and efficient. Whole genome sequencing may create greater challenges both in the technological and interpretational domains, and requires further reflection about the ethics of genetic testing in ART and PGD/PGS. Diagnostic laboratories should be reporting their results according to internationally accepted accreditation standards (ISO 15189). Further studies are needed in order to address issues related to the impact of ART on epigenetic reprogramming of the early embryo. LIMITATIONS, REASONS FOR CAUTION: The legal landscape regarding assisted reproduction is evolving, but still remains very heterogeneous and often contradictory. The lack of legal harmonization and uneven access to infertility treatment and PGD/PGS fosters considerable cross-border reproductive care in Europe, and beyond. WIDER IMPLICATIONS OF THE FINDINGS: This continually evolving field requires communication between the clinical genetics and IVF teams and patients to ensure that they are fully informed and can make well-considered choices. STUDY FUNDING/COMPETING INTERESTS: Funding was received from ESHRE, ESHG and EuroGentest2 European Union Coordination Action project (FP7 - HEALTH-F4-2010-26146) to support attendance at this meeting.

• MeSH
• akreditace MeSH
• asistovaná reprodukce škodlivé účinky   etika   zákonodárství a právo   trendy   MeSH
• dostupnost zdravotnických služeb MeSH
• embryonální kmenové buňky MeSH
• epigenomika MeSH
• lékařská genetika etika   zákonodárství a právo   trendy   MeSH
• lidé MeSH
• mužská infertilita genetika   MeSH
• nestabilita genomu MeSH
• preimplantační diagnóza etika   trendy   MeSH
• reprodukční lékařství etika   zákonodárství a právo   trendy   MeSH
• společnosti lékařské MeSH
• zdravotní turistika trendy   MeSH
• ženská infertilita genetika   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Evropa MeSH

Východisko. Mikrodelécie dlhého ramienka chromozómu Y patria k významným príčinám neobštrukčnej azoospermie a oligospermie. Cieľom tejto práce bolo zaviesť spoľahlivú molekulárne-genetickú metódu diagnostiky mikrodelécií chromozómu Y a zistiť zastúpenie jednotlivých typov mikrodelécií chromozómu Y u českých mužov so závažnými poruchami reprodukcie. Metódy a výsledky. Mikrodelécie chromozómu Y boli vyšetrované u 198 českých mužov s ťažkou poruchou reprodukcie so zníženým počtom spermií. Mikrodelécie chromozómu Y boli nájdené u 8/198 (4,0 %) vyšetrených mužov. Delécia v AZFc oblasti bola zistená v 62,5 % (5/8), kombinácia AZFc a AZFb delécie v 37,5 % (3/8) prípadov. Izolovaná delécia AZFb a ani delécia AZFa nebola nájdená. Závery. Určili sme zastúpenie jednotlivých typov mikrodelécií Y chromozómu u českých mužov s ťažkými poruchami reprodukcie. Bola zavedená štandardná molekulárne genetická diagnostika mikrodelécií chromozómu Y do rutinnej praxe.

Background. The Y chromosome microdeletions belong to the frequent genetical causes of male infertility. The aim of our studywas to introduce reliablemolecular genetic diagnosis of Ychromosomal microdeletions and to determine the prevalence of Y chromosomal microdeletions in Czech males with serious reproductive disorders. Methods and Results. The Ychromosomemicrodeletions were screened in 198Czech menwith serious reproductive disorders with decreased sperm count. The Y chromosome microdeletions were disclosed in 8/198 (4.0 %) examined males. The AZFc deletion type was revealed in 62,5 % (5/8) and the combined AZFc+b microdeletion in 37,5 % (3/8) of cases. Neither isolated AZFb nor AZFa microdeletion were found in any subject of the investigated group. Conclusions. Incidence of individual types of Y chromosomal microdeletions in Czech males with serious reproductive disorders was assessed. The standardisedmolecular genetic diagnosis ofYchromosomalmicrodeletions was introduced into the practice.

• MeSH
• asistovaná reprodukce MeSH
• chromozom Y genetika   MeSH
• chromozomální delece MeSH
• dospělí MeSH
• finanční podpora výzkumu jako téma MeSH
• genetické markery MeSH
• lidé MeSH
• mužská infertilita diagnóza   etiologie   MeSH
• polymerázová řetězová reakce MeSH
• spermatogeneze MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• přehledy MeSH
• srovnávací studie MeSH

PURPOSE: To analyse relationships between semen parameters, sperm chromatin integrity and frequencies of chromosomally unbalanced, disomic and diploid sperm in 13 Robertsonian and 37 reciprocal translocation carriers and to compare the results with data from 10 control donors. METHODS: Conventional semen analysis, Sperm Chromatin Structure Assay and FISH with probes for chromosomes involved in the individual translocations and for chromosomes X, Y, 7, 8, 13, 18 and 21. RESULTS: Normal semen parameters were found in 30.8 % of Robertsonian and 59.5 % of reciprocal translocation carriers. The rates of unbalanced sperm were 12.0 % in Robertsonian and 55.1 % in reciprocal translocation carriers with no difference between normospermic patients and those showing altered semen parameters. Significantly increased frequencies of spermatozoa showing defects in chromatin integrity and condensation, aneuploidy for chromosomes not involved in a translocation and diploidy were detected in translocation carriers with abnormal semen parameters. Normospermic reciprocal translocation carriers showed an increase in chromosome 13 disomy compared to the control group. There was no relationship between gametic and somatic aneuploidy in 12 translocation carriers studied by FISH on sperm and lymphocytes. The frequency of motile sperm was negatively correlated with the frequency of sperm showing disomy, diploidy and defective chromatin condensation. CONCLUSIONS: Abnormal semen parameters can serve as indicators of an additional risk of forming spermatozoa with defective chromatin and aneuploidy in translocation carriers.

• MeSH
• analýza spermatu MeSH
• aneuploidie MeSH
• chromatin genetika   MeSH
• dospělí MeSH
• heterozygot MeSH
• hybridizace in situ fluorescenční MeSH
• karyotypizace MeSH
• lidé středního věku MeSH
• lidé MeSH
• lidské chromozomy X MeSH
• lidské chromozomy, pár 13 MeSH
• lidské chromozomy, pár 18 MeSH
• lidský chromozom Y MeSH
• mužská infertilita genetika   MeSH
• segregace chromozomů MeSH
• sperma cytologie   MeSH
• spermie cytologie   MeSH
• translokace genetická genetika   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Sperm chromatin compaction in the sperm head is achieved when histones are replaced by protamines during spermatogenesis. Haploinsufficiency of the protamine 1 (PRM1) or PRM2 gene causes infertility in mice. However, the published data remain inconclusive about a role of PRM1/2 variants in male infertility and their association with semen parameters. By full sequence analysis, we assessed the frequency of sequence variations in PRM1 and PRM2 in three groups of Caucasian patients with idiopathic teratozoospermia and normal (n = 88) or reduced sperm concentration (n = 83) and in men with a high percentage of normal sperm morphology and normal concentrations (n = 77). Two rare (c.54G>A and c.102G>T) and one common SNP (c.230A>C) were identified in PRM1. In PRM2, some rare heterozygous mutations and the two common intronic SNPs 298G>C and 373C>A were detected. None of the PRM1/2 variants was associated with teratozoospermia or individually with other semen parameters. However, significant linkage disequilibrium was detected between the common SNPs of PRM1 and PRM2 which formed haplotypes. Analysis of the pooled group (n = 248) revealed that homozygous carriers of the common haplotype ACC had a twofold higher sperm concentration and count than men lacking this haplotype, with sperm counts of heterozygotes for ACC being midway between the homozygotes. This markedly decreased sperm output might either be caused by spermatozoa lacking the ACC haplotype not being viable, or subject to negative selection. In addition, a significant deviation from Hardy-Weinberg-Equilibrium of these SNPs might indicate natural selection in favour of the ACC allele which leads to higher sperm output and therefore better fertility. In conclusion, for the first time we describe an association of a common haplotype formed by PRM1 and PRM2 with sperm output in a large group of men.

• MeSH
• chromatin metabolismus   MeSH
• dospělí MeSH
• fertilita genetika   MeSH
• geny MeSH
• haplotypy MeSH
• heterozygot MeSH
• histony genetika   MeSH
• jednonukleotidový polymorfismus genetika   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mutace MeSH
• mužská infertilita genetika   MeSH
• protaminy * genetika   MeSH
• spermatogeneze genetika   MeSH
• spermie MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• práce podpořená grantem MeSH

Complex chromosomal rearrangements (CCR) represent rare structural chromosome abnormalities frequently associated with infertility. In this study, meiotic segregation in spermatozoa of an infertile normospermic carrier of a 4-breakpoint t(1;3;6) CCR was analysed. A newly developed array comparative genomic hybridization protocol was used, and all chromosomes in 50 single sperm cells were simultaneously examined. Three-colour FISH was used to analyse chromosome segregation in 1557 other single sperm cells. It was also used to measure an interchromosomal effect; sperm chromatin structure assay was used to measure chromatin integrity. A high-frequency of unbalanced spermatozoa (84%) was observed, mostly arising from the 3:3 symmetrical segregation mode. Array comparative genomic hybridization was used to detect additional aneuploidies in two out of 50 spermatozoa (4%) in chromosomes not involved in the complex chromosome rearrangement. Significantly increased rates of diploidy and XY disomy were found in the CCR carrier compared with the control group (P < 0.001). Defective condensation of sperm chromatin was also found in 22.7% of spermatozoa by sperm chromatin structure assay. The results indicate that the infertility in the man with CCR and normal spermatozoa was caused by a production of chromosomally unbalanced, XY disomic and diploid spermatozoa and spermatozoa with defective chromatin condensation.

• MeSH
• analýza jednotlivých buněk MeSH
• body zlomu chromozomu * MeSH
• dospělí MeSH
• genová přestavba * MeSH
• heterozygot MeSH
• hybridizace in situ fluorescenční MeSH
• lidé MeSH
• mužská infertilita etiologie   MeSH
• poruchy sexuálního vývoje s karyotypem 46, XY diagnóza   genetika   patologie   patofyziologie   MeSH
• profáze meiózy I MeSH
• segregace chromozomů * MeSH
• spermie patologie   MeSH
• srovnávací genomová hybridizace MeSH
• translokace genetická * MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH

Cieľ štúdie: Zavedenie metódy DNA analýzy AZF (Azoospermia factor region) oblasti Y chromozómu u mužov s poruchami plodnosti v Slovenskej republike. Typ štúdie: Genetická-prospektívna štúdia. Názov a sídlo pracoviska: Ústav lekárskej biológie, genetiky a klinickej genetiky LFUK a FNsP, Bratislava. Metodika: Detekcia bola robená pomocou polymerázovej reťazovej reakcie, použitím troch rôznych sád sY sekvencií. Na overenie niektorých typov delécií bol použitý fluorescenčne značený diagnostický set. Výsledky: Súbor tvorilo 822 mužov, z nich 349 s azoospermiou a 473 s oligospermiou. Delécie v AZF oblasti Y chromozómu boli potvrdené v 38 prípadoch (4,62 %). Z nich je 24 (6,88 %) pacientov zo súboru s azoospermiou a 14 zo súboru pacientov s oligospermiou (2,95 %). Zaznamenali sme delécie v každej z podoblastí AZF (a, b, c), kombinovanú AZFbc deléciu, ale aj deléciu celej AZF oblasti. Záver: Štúdia potvrdila, že detekcia mikrodelécii AZF oblasti má diagnostický a prognostický význam a poukázala na dôležitosť selekčných kritérií výberu pacientov.

Objective: Study on Y chromosomal AZF region deletions in Slovak population, application of DNA technique. Design: Genetic-prospective study. Setting: Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava. Methods: For detecting microdeletions in the Y-chromosomal AZF region in men with fertility disorders and for identifying Y-specific sequences we used the method of polymerase chain reaction (PCR) with using three different sets of sY sequences. For a verification of the specific type of deletion we used also fluorescently labeled kit. Results: Diagnoses of referred patients were divided into 2 groups: azoospermia, oligospermia. In the followed-up group of 822 patients there were 349 patients with azoospermia, 473 patients with oligospermia. Globally we reported 38 cases of deletions in the AZF region of the Y chromosome, i.e. 4.62%. 24 patients with deletion are from the group of patients with azoospermia, i.e. 6.88%, 14 patients are from the group of patients with oligospermia (2.95%). Considering particular types of deletions we recorded deletions in each region, AZFa, AZFb and AZFc, combinated AZFbc deletion, but also a complete deletion of the whole AZF region. Conclusion: The study confirmed that detection of microdeletions of the AZF region is significant from diagnostic and prognostic view and it pointed out the importance of selection criteria for selecting patients.

• MeSH
• azoospermie genetika   MeSH
• chromozomální delece MeSH
• dospělí MeSH
• lidé genetika   MeSH
• lidský chromozom Y genetika   MeSH
• mužská infertilita genetika   MeSH
• oligospermie genetika   MeSH
• polymerázová řetězová reakce MeSH
• proteiny semenné plazmy genetika   MeSH
• sekvenční analýza DNA MeSH
• Check Tag
• dospělí MeSH
• lidé genetika   MeSH
• mužské pohlaví MeSH

Toxoplasma gondii is a common protozoan parasite that infects warm-blooded animals throughout the world, including mice and humans. During infection, both, the parasite and the host, utilize various mechanisms to maximize their own reproductive success. Mice and humans are both the intermediate hosts for Toxoplasma gondii, which forms specialized vacuoles containing reproductive cysts in the formers' tissue. As half of the human population is infected, developing a disease called toxoplasmosis, along with an ever-growing number of couples suffering with idiopathic infertility, it is therefore surprising that there is a lack of research on how Toxoplasma gondii can alter reproductive parameters. In this study, a detailed histometric screening of the testicular function along with the levels of the pituitary luteinizing hormone (LH) were analysed in infected mice. Data on relative testis and epididymis weight, and sperm count were also collected. Based on the results obtained, the level of LH in the urine of Toxoplasma gondii infected mice was lower compared to the control. In direct correlation with the hormone level, testicular function and sperm production was also significantly lower in Toxoplasma gondii positive group using sperm count and histometric analysis as a marker. Not only were the number of leptotene primary spermatocytes and spermatids lowered, but the number of Sertoli cells and the tubule diameter were elevated. In parallel, a pilot epigenetic study on global testicular methylation, and specific methylation of Crem, Creb1 and Hspa1genes essential for successfully ongoing spermatogenesis was performed. Global methylation was elevated in Toxoplasma infected mice, and differences in the DNA methylation of selected genes were detected between the Toxoplasma positive and control group. These findings demonstrate a direct relation between Toxoplasma gondii infection and the decrease of male reproductive fitness in mice, which may contribute to an increase of idiopathic infertility in humans.

• MeSH
• CpG ostrůvky MeSH
• epididymis metabolismus   parazitologie   patologie   MeSH
• epigeneze genetická MeSH
• exprese genu MeSH
• genetická zdatnost genetika   MeSH
• interakce hostitele a parazita MeSH
• lidé MeSH
• luteinizační hormon genetika   metabolismus   MeSH
• metylace DNA MeSH
• modulátor elementu responzivního pro cyklický AMP genetika   metabolismus   MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• oligospermie MeSH
• protein vázající cAMP responzivní element genetika   metabolismus   MeSH
• proteiny tepelného šoku HSP70 genetika   metabolismus   MeSH
• semenotvorné kanálky metabolismus   parazitologie   patologie   MeSH
• Sertoliho buňky metabolismus   parazitologie   patologie   MeSH
• spermie metabolismus   patologie   MeSH
• Toxoplasma patogenita   fyziologie   MeSH
• toxoplazmóza zvířat genetika   metabolismus   parazitologie   patologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Damage to the genetic component of spermatozoa seems to play the main role in a majority of cases where current approaches fail to reveal the specific cause of male infertility. In this study, we compared semen quality in men assigned to two defined groups: men from couples with unexplained infertility - idiopathic infertility (A) and young men with no experiences of infertility (B). All samples were examined by standard ejaculate analysis and sperm chromatin structure assay (SCSA). Sperm chromatin damage was significantly higher in men from group A than in those from group B. Similar results were obtained by comparison of men from group A (all men were normozoospermic) with normozoospermic men from group B. According to these results, we can suppose that chromatin disorders may be the causal factor of subfertility or infertility in some of these men. No evidence for a strong association between chromatin disorders and standard parameters of ejaculates was found. We failed to confirm a relationship between smoking and sperm quality in men from any of the investigated groups. SCSA is a method that facilitates the identification of infertile men who otherwise show normal semen variables.

• MeSH
• analýza spermatu MeSH
• chromatin chemie   MeSH
• financování organizované MeSH
• lidé MeSH
• mužská infertilita patofyziologie   MeSH
• spermie cytologie   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH

Infertility is a compelling health problem. According to a recent meta-analysis, in average 9% of couples are infertile. Male factor is responsible for approximately half of these cases and has often genetic origin. Apart from azoospermia (no sperm), the phenotype of lowered sperm count and/or abnormal sperm morphology (oligo- and teratozoospermia) can be distinguished. Work on animal genetically defined models of infertility (including the work of our group), revealed many genes necessary for proper production of healthy sperm. Such results could not be achieved in man, but the potential clinical implications of these findings can only be estimated by replication of these results in man. We therefore propose to screen a cohort of cca 200 infertile men for mutations in selected genes that were proven to be necessary for normal sperm morphology in animal models. Our results may help in understanding the etiology of infertility in human, and improve genetic counseling of these patients.

Neplodnost je závažným zdravotním problémem. Podle recentní metaanalýzy je průměrně 9% párů neplodných. Neplodnost muže je zodpovědná asi za polovinu těchto případů a má často genetickou příčinu. Kromě azoospermie (žádné spermie), rozeznáváme snížený počet spermií a/nebo abnormální morfologii spermií (oligo- a teratozoospermie). Práce se zvířecími geneticky definovanými modely neplodnosti (včetně práce naší skupiny) odhalila řadu genů nezbytných ke správné morfogenezi zdravé spermie. Tyto výsledky nemohly být získány studiem lidské neplodnosti, nicméně, potenciální klinický význam těchto nálezů může být zjištěn jen replikací těchto výsledků u člověka. Proto navrhujeme screening kohorty neplodných mužů na výskyt mutací ve vybraných genech, které jsou prokazatelně nezbytné pro normální morfologii spermií u zvířecích modelů. Naše výsledky mohou pomoci v porozumění etiologii neplodnosti u člověka, a zlepšit genetické poradenství u těchto pacientů.

• MeSH
• kohortové studie MeSH
• krysa rodu rattus MeSH
• metaanalýza jako téma MeSH
• modely nemocí na zvířatech MeSH
• mutační analýza DNA MeSH
• mužská infertilita MeSH
• oligospermie MeSH
• spermatogeneze MeSH
• teratozoospermie MeSH
• Check Tag
• krysa rodu rattus MeSH

• Konspekt
• Biochemie. Molekulární biologie. Biofyzika
• NLK Obory
• genetika, lékařská genetika
• andrologie
• NLK Publikační typ
• závěrečné zprávy o řešení grantu IGA MZ ČR

Cíl studie: Porovnání nálezů pozitivních intra-akrosomálních proteinů a seminálních spermaglutinačníchprotilátek u pacientů, u párů s poruchou plodnosti a dárců.Typ studie: Prospektivní studie.Název a sídlo pracoviště: Gynekologicko-porodnická klinika LF UK a FN, Plzeň, Ústav molekulárnígenetiky AV ČR, Praha.Metodika: Použili jsme monoklonální protilátky (Hs-8 a Hs-14) proti intra-akrosomálním proteinůmlidských spermií (připraveny v Ústavu molekulární genetiky, AV ČR) k detekci akrosomálníchproteinů pomocí imunofl uorescenční metody. Mikroskopicky jsme hodnotili přítomnost,charakter a procento spermií s označenými intra-akrosomálními proteiny. Protilátky proti spermiímjsme určili přímým MAR-testem pro IgG, IgA, IgM a IgE. Vyšetřili jsme celkem 315 mužůz Poradny pro imunologii reprodukce a pacientů z IVF programu (od ledna 2002 do března 2003).Kontrolní skupinu tvořili dobrovolní dárci spermatu.Výsledky: U dárců jsme prokázali pozitivní intra-akrosomální proteiny i po rozmrazení ejakulátu,protilátky proti spermiím v seminální plazmě nebyly přítomny. Ve skupině normospermatikůjsme zjistili přítomnost seminálních spermaglutinačních protilátek a to v izotypu IgG u 11 %,v IgA ve 14,5 %, v IgM v 3,6 %, v IgE v 5,2 %. Silnou pozitivitu intra-akrosomálních proteinů značenýchmonoklonální protilátkou Hs8 jsme nalezli u 68,4 %, a značených monoklonální protilátkouHs14 dokonce u 81,3 % pacientů. Naproti tomu u oligostenospermatiků jsme prokázali výraznýnárůst spermaglutinačních protilátek (pro IgG ve 40,5 %, pro IgA u 28,6 %, pro IgM u 9,5 % a proIgE u 11,9 %). Intra-akrosomální proteiny byly nalezeny v dominantní podobě u 15,5 % (značenýchHs8) a u 20,2 % (značených Hs14).Závěr: Kvantitativní hodnocení intra-akrosomálních proteinů a sledování spermaglutinačníchprotilátek patří mezi významné ukazatele stavu lidských spermií a plodnosti muže.

Objective: Comparison of the positive intra-acrosomal proteins and spermagglutinating antibodiesin human semen samples from various groups of patients.Design: Prospective study.Setting: Department of Gynecology and Obstetrics and Faculty Hospital, Charles University, Pilsen,Institute of Molecular Genetics, Czech Academy of Science, Prague.Methods: Monoclonal antibodies Hs-8 and Hs-14 (prepared in the Institute of Molecular Genetics,Prague) were used for detection of intra-acrosomal sperm proteins. Microscopic immunofl uores cent methods detected the incidence, the character and the percentage of the spermatozoa specified by above-mentioned monoclonal antibodies. Direct mixed anti-immunoglobulin reactionstest (MAR-test) for IgG, IgM, IgA, IgE was used for detection of spermagglutinating antibody. Weexamined 315 infertile patients from Special Consultation for Immunology of Reproduction andfrom the IVF programme, and sperm healthy donors (January 2002 – March 2003).Results: Native donor‘s sperm cells had excellent positive intra-acrosomal proteins stained withmonoclonal antibodies Hs8 and Hs14 and after thawing as well as. No spermagglutinating antibodieswere found. In the group with normal sperm count and light microscopic morphology wefound the presence of seminal spermagglutinating antibodies in 11% (IgG), in 14.5% (IgA), in 3.6%(IgM), in 5.2% (IgE). Signifi cant positivity of intra-acrosomal protein stained with Hs8 monoclonalantibody was reached in 68.4%, and with Hs14 monoclonal antibody in even 81.3% of men. Onthe other hand, in oligoasthenospermatic patients we found signifi cant increasing of spermagglutinatingantibodies (for IgG 40.5%, for IgA 28.6%, for IgM 9.5%, for IgE 11.9%). Dominant goodstaining of intra-acrosomal proteins were seen only in 15.5% of men (for Hs8) and in 20.2% (forHs14).Conclusion: The quantitative detection of intra-acrosomal sperm proteins and spermagglutinatingantibodies are used as important properties of human semen and serve for evaluation ofacrosomal state, and male fertility together.

• MeSH
• akrozom cytologie   chemie   MeSH
• dospělí MeSH
• fertilizace in vitro MeSH
• finanční podpora výzkumu jako téma MeSH
• imunologické testy metody   MeSH
• lidé MeSH
• mužská infertilita diagnóza   etiologie   terapie   MeSH
• proteiny MeSH
• protilátky MeSH
• spermie patologie   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• přehledy MeSH
• srovnávací studie MeSH