• MeSH
• Fluoresceins diagnostic use   MeSH
• Myocardial Infarction pathology   MeSH
• Ischemia MeSH
• Cardiopulmonary Bypass MeSH
• Coronary Vessels MeSH
• Myocardium pathology   MeSH
• Dogs MeSH
• Animals MeSH
• Check Tag
• Dogs MeSH
• Animals MeSH
• Publication type
• Comparative Study MeSH

Cíl. Cílem studie bylo stanovit diagnostickou výtěžnost kontrastní MR angiografie (MRA) s použitím paralelních akvizičních technik v diagnostice stenózy renální tepny. Metoda. Celkem bylo vyšetřeno 70 nemocných s hypertenzní chorobou a podezřením na stenózu renální tepny. Vyšetření byla prováděna na přístroji Magnetom Symphony Maestro Class 1,5 T s použitím array cívek. Průchod bolusu kontrastní látky byl monitorován metodou bolus tracking. Parametry MRA sekvence byly: TR 3,7 ms; TE 1,2 ms; flip angle 25o; akviziční doba 18 s; velikost voxelu 1,1× 1,0×1,1 mm; centrický náběr dat v k-prostoru; paralelní akviziční techniky s akceleračním faktorem 2 (GRAPPA). Za hemodynamicky významnou byla považována stenóza 60 % a více. Výsledky MRA byly porovnávány s nálezem na digitální subtrakční angiografii, která sloužila jako zlatý standard. Výsledky. Senzitivita a specificita MRA v detekci hemodynamicky významné stenózy renální tepny byla 92 %, respektive 95 %. Její prevalence v našem souboru činila 37 %. Závěr. Při splnění parametrů dostatečného geometrického rozlišení vykazuje kontrastní MR angiografie vysokou senzitivitu a specificitu v diagnostice stenózy renální tepny.

Aim. The aim of our study was to assess the diagnostic value of contrast-enhanced MR angiography (MRA) utilizing parallel acquisition techniques in the detection of renal artery stenosis. Method. Seventy hypertensive subjects with suspected renal artery stenosis were examined on a 1.5 Tesla MR system with body array coil. Bolus tracking was used to monitor the arrival of contrast agent to the abdominal aorta. The MRA sequence parameters were as follows: TR 3.7 ms; TE 1.2 ms; flip angle 25°; acquisition time 18 s; voxel size 1.1 × 1.0 × 1.1 mm; centric k-space sampling; parallel acquisition techniques with acceleration factor of 2 (GRAPPA). Renal artery stenosis of 60 % and more was considered hemodynamically significant. The results of MRA were compared to digital subtraction angiography serving as a standard of reference. Results. Sensitivity and specificity of MRA in the detection of hemodynamically significant renal artery stenosis were 92 % and 95 %, respectively. Its prevalence was 37 % in our study population. Conclusion. Contrast-enhanced MRA with high spatial resolution offers sufficient sensitivity and specificity for screening of renal artery stenosis.

• MeSH
• Diagnostic Techniques, Urological utilization   MeSH
• Angiography, Digital Subtraction methods   utilization   MeSH
• Humans MeSH
• Magnetic Resonance Angiography methods   instrumentation   utilization   MeSH
• Renal Artery Obstruction diagnosis   etiology   physiopathology   MeSH
• Hypertension, Renal diagnosis   MeSH
• Sensitivity and Specificity MeSH
• Check Tag
• Humans MeSH
• Publication type
• Comparative Study MeSH

• MeSH
• Cervix Mucus analysis   MeSH
• Immunoglobulin E analysis   MeSH
• Immunologic Techniques MeSH
• Infertility, Female diagnosis   MeSH

• MeSH
• Molecular Diagnostic Techniques MeSH
• Cross Infection diagnosis   MeSH
• Communicable Diseases diagnosis   MeSH
• Microbiological Techniques MeSH
• Flow Cytometry MeSH
• Virus Diseases diagnosis   MeSH

• Conspectus
• Biochemie. Molekulární biologie. Biofyzika
• NML Fields
• biologie
• infekční lékařství
• mikrobiologie, lékařská mikrobiologie
• NML Publication type
• www dokumenty

The review deals with osmium tetroxide, a very toxic volatile substance. It can be misused as a chemical weapon intoxicating by inhalation in closed space with limited air circulation (airports, shopping centres). In contrast, osmium tetroxide is very important compound in analytical chemistry especially in electron microscopy and DNA labelling in biophysical chemistry. Some analytical techniques for its detection are discussed with an emphasis on voltammetric methods.

• MeSH
• Chemical Warfare Agents MeSH
• Chemistry Techniques, Analytical MeSH
• DNA Restriction-Modification Enzymes MeSH
• Electrochemical Techniques MeSH
• Microscopy, Electron MeSH
• Osmium Tetroxide analysis   chemistry   adverse effects   MeSH

Objective: The objective of this work is to develop efficient classification systems using intelligent computing techniques for classification of normal and abnormal EEG signals. Methods: In this work, EEG recordings were carried out on volunteers (N=170). The features for classification of clinical EEG signals were extracted using wavelet transform and the feature selection was carried out using Principal Component Analysis. Intelligent techniques like Back Propagation Network (BPN), Adaptive Neuro-Fuzzy Inference System (ANFIS), Particle Swarm Optimization Neural network (PSONN) and Radial Basis function Neural network (RBFNN) were trained for diagnosing seizures. Further, the performance of the developed classifiers was compared. Results: Results demonstrate that RBFNN classifies normal and abnormal EEG signals better than the other methods. It appears that the RBFNN is able to detect Generalized Tonic-Clonic Seizure (GTCS) more efficiently than the Complex Partial Seizures (CPS). Positive predictive value was better in PSONN and ANFIS than BPN method. Conclusions: It appears that the combination of Wavelet transform method and PCA derived features along with RBFNN classifier is efficient for automated EEG signal classification.

• MeSH
• Electroencephalography * classification   instrumentation   statistics & numerical data   MeSH
• Epilepsy * diagnosis   classification   MeSH
• Humans MeSH
• Computers * statistics & numerical data   utilization   MeSH
• Computer Systems statistics & numerical data   utilization   MeSH
• Statistics as Topic MeSH
• Check Tag
• Humans MeSH

• MeSH
• Cell Death MeSH
• Cytological Techniques methods   MeSH
• Medical Oncology MeSH
• Flow Cytometry methods   MeSH
• Publication type
• Review MeSH

Heavy metals rank among the most toxic compounds occurring in the environment; they are also dangerous due to bioaccumulation. Plants and animals have developed a number of protective mechanisms. The detoxification mechanisms of heavy metals in different organisms have been intensively studied for many years. We aimed at investigation of detoxification mechanisms of maize plants treated with 0, 50, 100, 150, 200, 400 and 500 µM Cd(II) solutions for six days. In particular, we observed their growth and determined the Cd content (by differential pulse anodic stripping voltammetry) and thiol concentration (by HPLC) in the treated plants. Maize plants took up 6 pg of Cd per gram per hour at the lowest dose and 23 pg Cd per g per hour at the highest Cd dose. The relations of glutathione and phytochelatin contents, applied Cd dose, cultivation time, growth curve and plant morphology were investigated.

• MeSH
• Electrochemistry methods   MeSH
• Financing, Government MeSH
• Plants, Edible MeSH
• Cadmium analysis   adverse effects   MeSH
• Zea mays metabolism   MeSH
• Soil Pollutants MeSH
• Sulfhydryl Compounds analysis   MeSH

• MeSH
• Influenza, Human diagnosis   microbiology   MeSH
• Fluorescent Antibody Technique MeSH
• Humans MeSH
• Orthomyxoviridae isolation & purification   MeSH
• RNA, Viral analysis   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Comparative Study MeSH

The developments in the field of capacitively coupled contactless conductivity detection in the approximate period from July 2010 to June 2012 are traced. Few reports concerning fundamental studies or new detector designs have appeared. On the other hand, applications in standard CZE are flourishing and contactless conductivity measurements are increasingly being employed as part of novel or more sophisticated experimental systems. Work on the lab-on-chip devices integrating contactless conductivity detection is continuing. A range of reports on the use of the simple yet powerful detection technique of contactless conductivity measurements in chromatographic separation as well as for analytical methods not including a separation step have also appeared.

• MeSH
• Food Analysis methods   MeSH
• Electric Conductivity MeSH
• Electrophoresis, Capillary instrumentation   methods   trends   MeSH
• Electrophoresis, Microchip methods   MeSH
• Chemistry, Clinical instrumentation   methods   MeSH
• Drug Contamination prevention & control   MeSH
• Pharmaceutical Preparations analysis   MeSH
• Microfluidic Analytical Techniques instrumentation   methods   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Review MeSH