Metal-polluted soils represent hostile environments affecting the composition and functions of soil microbial communities. This study evaluated the implication of combining the mycoremediated dry olive residue (MDOR) amendment application with the inoculation of the arbuscular mycorrhizal fungi (AMF) Funneliformis mosseae in restoring the quality, composition, and functionality of soil microbial communities. To achieve this aim, a mesocosms experiment was set up that included three variations: i) with and without application of Penicillium chrysogenum-10-transformed MDOR (MDOR_Pc), and Chondrosterum purpureum-transformed MDOR (MDOR_Cp) amendments; ii) with and without F. mosseae inoculation; and iii) 30-day and 60-day soil treatment time. As a result of this combined treatment, changes in the soil labile organic C and N fractions were observed throughout the experiment. Increases in the abundance of phospholipid fatty acids (PLFAs) for bacteria, actinobacteria, and Gram- and Gram+ bacteria were also recorded at the end of the experiment. The addition of MDOR amendments boosted fungal and AM fungi communities. AM fungi root and soil colonization was also enhanced as the result of improvement nutrient turnover and spatial conditions caused by adding MDOR in combination with an inoculation of F. mosseae. The composition and functionality of microbial communities seemed to be an important ecological attribute indicating an apparently fully functional restoration of this metal-polluted soil and therefore suggesting the suitability of the combined MDOR and AM fungus treatment as a reclamation practice.

• MeSH
• houby MeSH
• kovy MeSH
• látky znečišťující půdu * MeSH
• mikrobiota * MeSH
• mykorhiza * MeSH
• Olea * MeSH
• půda MeSH
• půdní mikrobiologie MeSH
• Publikační typ
• časopisecké články MeSH

For our experiments we selected two oncogenic, bcr-abl-transformed mouse cell lines, viz. B210 and 12B1. Both cell types are capable of inducing leukemia-like disease in syngeneic BALB/c mice after intravenous inoculation. 12B1 cells can moreover form solid tumors after subcutaneous injection. Since immunotherapy would expectedly be most effective in animals in which the tumor mass had been reduced by other therapeutic means, we attempted to develop a combined therapeutic system for suppressing tumor growth. In the present study, mice inoculated with the aggressive 12B1 cells were treated with imatinib mesylate (IM), mouse interferon alpha (IFNalpha) and cyclophosphamide (Cy) in combination with genetically modified tumor cells engineered to produce various cytokines. These cell vaccines had been derived from B210 cells. Therapy with IM or IFNalpha alone or cell immunotherapy alone resulted in partial suppression of tumor growth. Of the different therapeutic regimens tested, a combination of repeated doses of IM, IFNalpha and cell vaccines with one relatively high dose of Cy (200 mg/kg) was the most effective, resulting in tumor-free survival of a large portion of mice. The spleens, livers and bone marrows of the successfully treated animals were tested for the presence of bcr-abl-positive cells by means of RT-PCR technique. Results were negative, this suggesting that the animals had been cleared of residual disease.

• MeSH
• bcr-abl fúzní proteiny imunologie   terapeutické užití   MeSH
• cyklofosfamid aplikace a dávkování   MeSH
• experimentální nádory imunologie   terapie   MeSH
• faktor stimulující granulocyto-makrofágové kolonie biosyntéza   MeSH
• financování organizované MeSH
• imunoterapie metody   MeSH
• interferon gama aplikace a dávkování   MeSH
• interleukin-12 biosyntéza   MeSH
• interleukin-2 biosyntéza   MeSH
• kombinovaná terapie MeSH
• myši inbrední BALB C MeSH
• myši MeSH
• piperaziny aplikace a dávkování   MeSH
• polymerázová řetězová reakce MeSH
• protinádorové látky aplikace a dávkování   MeSH
• protinádorové vakcíny imunologie   MeSH
• pyrimidiny aplikace a dávkování   MeSH
• transformované buněčné linie MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH

Oregano essential oil (OEO) and caprylic acid (CA) are highly aromatic natural antimicrobials with limited individual application in food. We proved their combined additive effect when used in meat. Application of 0.5% CA and 0.2% OEO (v/w) with 0.1% of citric acid in vacuum packed minced beef inoculated with Listeria monocytogenes at a concentration of 5 log cells/g reduced counts of lactic acid bacteria by 1.5 log CFU/g and counts of psychrotrophic bacteria and L. monocytogenes by more than 2.5 log CFU/g at the end of storage at 3°C for 10 days. In sensory evaluation the samples with OEO showed during the whole experiment statistically better scores than control, whereas the samples treated with CA showed worse colour attributes.

• MeSH
• antiinfekční látky farmakologie   MeSH
• barva MeSH
• chemické jevy MeSH
• dobromysl (rod) chemie   MeSH
• kapryláty farmakologie   MeSH
• koncentrace vodíkových iontů MeSH
• kontaminace potravin prevence a kontrola   MeSH
• konzervace potravin MeSH
• Lactobacillaceae účinky léků   růst a vývoj   MeSH
• lidé MeSH
• Listeria monocytogenes účinky léků   růst a vývoj   MeSH
• maso analýza   mikrobiologie   MeSH
• obaly potravin MeSH
• odoranty analýza   MeSH
• oleje prchavé farmakologie   MeSH
• počet mikrobiálních kolonií MeSH
• potravinářská mikrobiologie MeSH
• skot MeSH
• spotřebitelská bezpečnost produktů MeSH
• vakuum MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• skot MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Oncolytic virotherapy is a novel approach to cancer treatment. In the present study we tested the ability of reovirus type 3, strain Dearing, to suppress the growth of tumors induced in mice by HPV16-transformed TC-1 cells. In vitro, these cells are highly susceptible to the virus. In repeated in vivo tests the intratumoral inoculation of the virus resulted in only a minor slow-down of tumor growth, never in a complete cure. The effect of the treatment was not enhanced by the simultaneous administration of non-oncogenic, genetically modified TC-1 cells expressing either IL-2, IL-12 or GM-CSF, and, in fact, the oncolytic effect of the virus was even less expressed in some instances. When cyclophosphamide was used in combination with the viral treatment, a synergistic effect resulting in tumor suppression was observed. In most instances the tumor regression was transitory, however, and was followed by tumor progression. The outcome of these experiments was dependent on the timing of the two treatments.

• MeSH
• cyklofosfamid aplikace a dávkování   MeSH
• experimentální nádory terapie   virologie   MeSH
• faktor stimulující granulocyto-makrofágové kolonie genetika   MeSH
• financování organizované MeSH
• genetická terapie metody   MeSH
• interleukin-12 genetika   MeSH
• interleukin-2 genetika   MeSH
• kombinovaná terapie MeSH
• lidský papilomavirus 16 MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• onkolytická viroterapie metody   MeSH
• protinádorové látky aplikace a dávkování   MeSH
• protinádorové vakcíny aplikace a dávkování   MeSH
• savčí orthoreovirus 3 MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH

Mineral nutrition of crop plants is one of the major challenges faced by modern agriculture, particularly in arid and semi-arid regions. In alkaline calcareous soils, the availability of phosphorus and zinc is critically less due to their fixation and precipitation as complexes. Farmers use fertilizers to fulfill crop requirements, but their efficacy is less, which increases production costs. Plant growth-promoting rhizobacteria (PGPR) can improve the availability of crop nutrients through solubilizing the insoluble compounds of phosphorus and zinc in soil. In the present study, a total of 40 rhizobacterial isolates were isolated from cotton rhizosphere and screened for improving cotton growth through the solubilization of phosphorus and zinc. Out of these 40 isolates, seven isolates (IA2, IA3, IA6, IA7, IA8, IA13, and IA14) efficiently solubilized insoluble rock phosphate while seven isolates (IA10, IA16, IA20, IA23, IA24, IA28, and IA30) were more efficient in solubilizing insoluble zinc oxide. In liquid media, strain IA7 (2.75 μg/mL) solubilized the highest amount of phosphate while the highest concentration of soluble zinc was observed in the broth inoculated with strain IA20 (3.94 μg/mL). Seven phosphate-solubilizing and seven zinc-solubilizing strains were evaluated using jar trial to improve the growth of cotton seedlings, and the results were quite promising. All the inoculated treatments showed improvement in growth parameters in comparison with control. Best results were shown by the combined application of IA6 and IA16, followed by the combination of strains IA7 and IA20. Based on the jar trial, the selected isolates were further characterized by plant growth-promoting characters such as siderophores production, HCN production, ammonia production, and exopolysaccharides production. These strains were identified through 16S rRNA sequencing as Bacillus subtilis IA6 (accession # MN005922), Paenibacillus polymyxa IA7 (accession # MN005923), Bacillus sp. IA16 (accession # MN005924), and Bacillus aryabhattai IA20 (accession # MN005925). It is hence concluded that the integrated use of phosphate-solubilizing and zinc-solubilizing strains as potential inoculants can be a promising approach for improving cotton growth under semi-arid conditions.

• MeSH
• Bacillus klasifikace   genetika   izolace a purifikace   metabolismus   MeSH
• fosfáty metabolismus   MeSH
• fosfor metabolismus   MeSH
• fylogeneze MeSH
• Gossypium růst a vývoj   mikrobiologie   MeSH
• očkovadla agrotechnická klasifikace   genetika   izolace a purifikace   metabolismus   MeSH
• Paenibacillus polymyxa klasifikace   genetika   izolace a purifikace   metabolismus   MeSH
• půda chemie   MeSH
• půdní mikrobiologie MeSH
• rhizosféra MeSH
• RNA ribozomální 16S genetika   MeSH
• zinek metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH

Cíl práce: Diagnostika Bartonella henselae metodou polymerázové řetězové reakce (PCR) v exstirpovaných uzlinách 10 pacientů se sérologickým průkazem nemoci z kočičího škrábnutí. Materiál a metody: Soubor z období 2015–2018 zahrnoval 10 pacientů se sérologicky prokázanou nemocí z kočičího škrábnutí, všichni pacienti měli pozitivitu IgG protilátek proti B. henselae, 6 pacientů současně i pozitivitu IgM protilátek. Jednalo se o 4 muže a 6 žen, celkem 7 dětí a 3 dospělé ve věkovém rozmezí 5–52 let. Vyšetřeno bylo 11 exstirpovaných uzlin uložených v parafinových blocích od 10 pacientů, varianty granulomatózního zánětu byly zjištěny u 9 pacientů, chlapec ve věku 13 let měl Hodgkinův lymfom. Pro izolaci DNA byla použita komerční souprava cobas ® DNA Sample preparation Kit (Roche) a pro detekci DNA Bartonella sp. metodou real-time PCR komerční souprava BactoReal® Kit Bartonella spp. (Ingenetix) využívající detekci gltA genu specifického pro rod Bartonella. Výsledky: Průkaz bartonely v histologickém preparátu metodou PCR byl pozitivní či hraničně pozitivní u 4 z 10 pacientů. U jednoho pacienta byly vyšetřeny 2 uzliny s pozitivním výsledkem jen v jedné uzlině. Výsledek byl pozitivní u dospělého muže, slabě pozitivní u 3 dětí. Dva z těchto 4 pacientů měli histologický nález granulomatózně-abscedujícího a 2 pacienti granulomatózně-nekroticko-abscedujícího zánětu. Pozitivita či hraniční pozitivita PCR byla zjištěna u 4 pacientů s pozitivitou protilátek třídy IgM proti B. henselae, ale u žádného pacienta s negativitou IgM protilátek. Rovněž chlapec s lymfomem měl negativní výsledek PCR diagnostiky. Závěr: Kombinace sérologického vyšetření s histologickým vyšetřením uzlin a PCR metodou může zlepšit diagnostiku nemoci z kočičího škrábnutí.

Objective: The diagnosis of Bartonella henselae by polymerase chain reaction (PCR) in lymph nodes removed in 10 patients with serologically confirmed evidence cat-scratch disease. Material and methods: The 2015–2018 group consisted of 10 patients with serologically confirmed cat-scratch disease, all of them having positive IgG antibodies and 6 patients also positive IgM antibodies against B. henselae. The group included 4 men and 6 women, 7 children and 3 adults, aged 5–52 years. Eleven lymph nodes obtained from the 10 patients were formalin-fixed paraffin-embedded. Variants of granulomatous inflammation were found in 9 patients; a 13-year-old boy had Hodgkin’s lymphoma. DNA isolation was per- formed with cobas ® DNA Sample Preparation Kit (Roche). DNA of Bartonella spp. was detected by real-time PCR with BactoReal ® Kit Bartonella spp. (Ingenetix) detecting the gltA gene specific for the genus Bartonella. Results: Four of the 10 patients tested positive or borderline positive for Bartonella when their histological material was analyzed by PCR. One patient with 2 lymph nodes examined showed a positive result for only 1 lymph node. One adult male had a positive result; three children showed borderline positive results. Of those, two patients had suppurative granulomatous and the other 2 patients had necrotizing suppurative granulomatous inflammation as histological findings. All 4 patients had positive IgM antibodies against B. henselae. The boy with lymphoma had a negative PCR result. Conclusion: Serological tests combined with histological examination of lymph nodes and PCR may improve the diagnosis of cat-scratch disease.

• MeSH
• lidé MeSH
• nemoc z kočičího škrábnutí * diagnóza   MeSH
• polymerázová řetězová reakce metody   MeSH
• retrospektivní studie MeSH
• Check Tag
• lidé MeSH

• MeSH
• interferon alfa biosyntéza   MeSH
• leukocyty imunologie   MeSH
• transfuze leukocytů MeSH
• transplantace heterologní MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH

Four laboratory-hatched European kestrels Falco tinnunculus L. were fed on laboratory mice and common voles Microtus arvalis Pallas previously inoculated with different doses of sporulated oocysts of Caryospora kutzeri Böer, 1982. Two kestrels that were fed infected mice shed C. kurtzeri oocysts 6 days after ingesting murine tissues. To compare direct and indirect transmissions, two of the kestrels were subsequently directly inoculated with 10(5) sporulated C. kutzeri oocysts and became patent on days 8 and 9 and shed caryosporan oocysts up to day 25 post inoculation. Additionally, four mice were inoculated with 10(6) oocysts in order to examine mouse tissues for the presence of developmental stages of C. kutzeri. No coccidian stages were found in the tissues of inoculated mice. The experiment showed that developmental stages of C. kutzeri are able to survive in mouse tissues and cause infection of suitable host after their ingestion.

• MeSH
• Arvicolinae parazitologie   MeSH
• dravci parazitologie   MeSH
• Eimeriidae * izolace a purifikace   MeSH
• feces parazitologie   MeSH
• hlodavci parazitologie   MeSH
• kokcidióza parazitologie   přenos   veterinární   MeSH
• myši SCID MeSH
• myši MeSH
• nemoci ptáků parazitologie   přenos   MeSH
• organismy bez specifických patogenů MeSH
• přenos infekční nemoci * MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

The GBS/ATM overlap is characterized by the simultaneous occurrence of Guillain-Barré syndrome (GBS) and acute transverse myelitis (ATM), which are two neurological autoimmune disorders. In this context, cat scratch disease (CSD) was rarely reported combined with this overlap. An adult female presenting fever, back pain, inferior limb weakness, and anuria was admitted to our hospital. On the physical exam, a distended bladder and bilateral lymphadenopathy were observed. The neurological assessment revealed muscle weakness, plantar flexion, and hyporeflexia in right with absence in left. Also, she reported hyperalgesia in inferior limbs. Her blood pressure was fluctuating being in the majority of the time hypertensive. A spinal cord MRI (magnetic resonance imaging) was suggestive of transverse myelitis. Methylprednisolone was started. The cerebrospinal fluid showed 37.0 cells/mm3 of white blood cell count, 49 mg/dl of glucose, and 50.7 mg/dl of protein. Ceftriaxone and vancomycin were started. On further questioning, the subject stated that her finger was bitten by a cat about two weeks before the beginning of the symptoms. Serological tests were positive for Bartonella henselae. Doxycycline and rifampin were started. After one-month, her symptoms improve but she continued with a radicular pain and weakness. An EMG (electroneuromyography) was suggestive of demyelination. IVIG (intravenous immunoglobulin) was started. After IVIG 4-day, the patient had recovery of her strength. To the authors' knowledge, there are two case reports of pediatric individuals linking CSD and GBS/ATM. Still, this association in an adult patient has not been reported until the present moment.

• MeSH
• Bartonella henselae * MeSH
• dospělí MeSH
• Guillainův-Barrého syndrom * MeSH
• lidé MeSH
• magnetická rezonanční tomografie MeSH
• nemoc z kočičího škrábnutí * MeSH
• transverzální myelitida * MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH

Encephalitozoon cuniculi is probably the most common microsporidia which infects a wide range of vertebrates, including human. So far, four genotypes of this parasite have been identified based on the rRNA internal transcribed spacer variations. The course of infection caused by E. cuniculi III had very massive onset in immunocompetent host characterized by the presence of this parasite in all organs and tissues within one week after peroral infection. Encephalitozoonosis caused by E. cuniculi III had very progressive spreading into all organs within first week post inoculation in immunocompromised SCID mice and led to the death of the host. The experimental treatment with albendazole of immunocompetent BALB/c mice infected with E. cuniculi III have shown very weak effect. Our findings clearly showed that the different course of infection and response to treatment depends not only on the immunological status of the host, but also on the genotype of microsporidia. It could be very important especially for individuals under chemotherapy and transplant recipients of organs originating from infected donors.

• MeSH
• albendazol farmakologie   terapeutické užití   MeSH
• antifungální látky farmakologie   terapeutické užití   MeSH
• Encephalitozoon cuniculi účinky léků   genetika   imunologie   fyziologie   MeSH
• encephalitozoonóza farmakoterapie   imunologie   parazitologie   MeSH
• feces parazitologie   MeSH
• genotyp MeSH
• imunokompetence * MeSH
• imunokompromitovaný pacient * MeSH
• myši inbrední BALB C MeSH
• myši SCID MeSH
• myši MeSH
• spory hub MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH