Aplikace probiotik v prevenci a léčbě dysmikrobií je dlohodobě studována. Aplikace na sliznici nosu a nosohltanu dosud nebyla publikována. Po vyzkoušení přípravku probiotika u dospělých dobrovolníků, byla provedena aplikace u dětí. U 16 z 18 dětí (0,5–13 let) s rozvíjejícím se zánětem horních dýchacích cest došlo k ústupu potíží, bez nutnosti nasadit antibiotika. Nedošlo k žádné nežádoucí příhodě.

Usefulness of probiotics in prevention and treatment of non-microbial disorders is studied for a long time. Intranasal and nasopharyngeal application was not published yet. We have studied application in children after check-out of it in adult volunteers. In 16 from 18 children (0.5–13 years) suffering from acute nasopharyngeal inflammation we observed improvement of their health status without necessity of antibiotics treatment. There were no adverse reactions.

• MeSH
• antipyretika MeSH
• aplikace intranazální * MeSH
• dítě MeSH
• dospělí MeSH
• infekce dýchací soustavy * terapie   MeSH
• kojenec MeSH
• Lactobacillus acidophilus * MeSH
• lidé MeSH
• mikrobiologie MeSH
• předškolní dítě MeSH
• probiotika * aplikace a dávkování   MeSH
• sliznice MeSH
• směrnice pro lékařskou praxi jako téma MeSH
• výsledek terapie MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• kojenec MeSH
• lidé MeSH
• předškolní dítě MeSH
• Publikační typ
• práce podpořená grantem MeSH

Úvod: Keďže v súčasnej dobe narastá počet alergických ochorení, hľadajú sa nové spôsoby ich prevencie a liečby. Kľúčovú úlohu na vzniku alergie zohráva narušená črevná mikroflóra, ktorá nemá dostatočný protektívny vplyv na ochranu črevnej bariéry. Následne dochádza k deštrukcii tight junction spojení alergickým zápalom a vzostupu črevnej permeability. Adekvátnou probiotickou suplementáciou dochádza k obnove mikrobiálnej, ale aj ďalších bariér čreva a k zlepšeniu prejavov alergie. Cieľ štúdie: Cieľom tejto pilotnej prospektívnej štúdie je zistiť vplyv podávania Lactobacillus rhamnosus GG na črevnú permeabilitu a prejavy atopickej dermatitídy u alergických detí. Stanoviť rozdiely v črevnej permeabilite u alergických a nealergických detí a korelovať tieto výsledky s anamnestickými údajmi. Materiál a metodika: Pilotnej štúdie sa zúčastnilo 39 detí (22 alergických a 17 zdravých detí) vo veku 3 mesiace až 3 roky, u ktorých bol realizovaný test črevnej permeability, stanovený index permeability (IP) a vyplnený formulár so základnými anamnestickými údajmi. V skupine alergikov boli počas 6 týždňov podávané probiotické kvapky obsahujúce Lactobacillus rhamnosus GG v účinnej koncentrácii 109 CFU. Po 6 týždňoch bol opäť realizovaný test črevnej permeability. V skupine alergikov bolo stanovené skóre atopickej dermatitídy (SCORAD) pred a po podávaní probiotík. Jednotlivé hodnoty boli porovnávané a štatisticky vyhodnotené prostredníctvom neparametrických testov – Mannov-Whitneyho U-test a Wilcoxonov test, korelačná analýza bola realizovaná podľa Spearmana. Výsledky: V skupine alergických detí signifikante poklesol IP (p <0,001) a SCORAD (p <0,05) po 6-týždňovej suplementácii Lactobacillus rhamnosus GG. Bola zaznamenaná pozitívna korelácia medzi hodnotami IP a závažnosťou atopickej dermatitídy (p <0,05). Medzi alergickou a kontrolnou skupinou neboli zaznamenané signifikantné rozdiely v spôsobe pôrodu, dĺžke dojčenia a zavádzania nemliečnych príkrmov. V alergickej skupine malo 89,3 % detí pozitívnu alergickú anamnézu (u oboch rodičov 66,6 %, u jedného rodiča 22,7 %) v kontrolnej skupine bola pozitívna rodinná anamnéza len zo strany 1 rodiča (29,4 %). Záver: V našej pilotnej štúdii sme potvrdili vplyv Lactobacillus rhamnosus GG na zníženie črevnej permeability a zlepšenie prejavov atopickej dermatitídy. Otázkou však zostáva, či po vysadení probiotickej suplementácie budú tieto zmeny pretrvávať aj naďalej.

Background: Since there is an increasing number of allergic diseases these days, scientists are looking for new ways of prevention and treatment. Disturbed intestinal microflora which lost sufficient protective effect on intestinal barrier plays key role in the development of allergy. Allergic inflammation destroys tight junction and increses intestinal permeability. Adequate probiotic supplementiation renews microbial as well as other intestinal barriers and improves the symptoms of allergy. Aim: The objective of this prospective pilot study is to investigate the effects of administration of Lactobacillus rhamnosus GG on intestinal permeability and symptoms of atopic dermatitis in allergic children. Also it is to determine the differences in intestinal permeability in allergic and non-allergic children and to correlate these results with anamnestic data. Materials and methods: The pilot study has been conducted on 39 children (22 allergic and 17 healthy children) aged 3 months to 3 years to whom was performed lactulose-manitol intestinal permeability test, determinated lactulose-mannitol (L/M) ratio and completed paper form of basic anamnestic data . In the allergic group of children have been administered for 6 weeks probiotic drops containing Lactobacillus rhamnosus GG in an effective concentration 109 CFU. After 6 weeks the permeability test was performed again. In the allergic group was established score of atopic dermatitis (SCORAD) before and after administration of probiotics. The results were statistically analyzed and evaluated using nonparametric Mann-Whitney U-test and Wilcoxon rank test. Correlation analysis was performed by Spearman. Results: In the allergic group after six weeks of supplementation of Lactobacillus rhamnosus GG there has been significant decrease of L/M ratio (p<0.001) and SCORAD (p<0.05). There was a positive correlation between the L/M ratio and the clinical severity of eczema (p<0.05). There were not any significant differences among allergic and control group in delivery method, length of breast-feeding or introduction of non-milk based foods. In the allergic group 89.3% of children had positive family allergic history (in both parents 66.6%, in one parent 22.7%), and in the control group there was positive family history in 29.4% of children (only in one parent) (p<0.001). Conculsions: In our pilot study, we have confirmed the effect of Lactobacillus rhamnosus GG on reduction of intestinal permeability and improvement of the symptoms of atopic dermatitis. The question is whether these changes will persist after the withdrawal of probiotic supplementation. Key words : intestinal permeability, lactulose-mannitol (L/M) ratio, Lactobacillus rhamnosus GG (LRH 08), SCORAD, atopic dermatitis

• Klíčová slova
• střevní permeabilita, index permeability,
• MeSH
• anamnéza MeSH
• atopická dermatitida * farmakoterapie   MeSH
• intestinální absorpce účinky léků   MeSH
• kojenec MeSH
• Lacticaseibacillus rhamnosus * MeSH
• laktulosa moč   MeSH
• lidé MeSH
• mannitol moč   MeSH
• neparametrická statistika MeSH
• permeabilita MeSH
• pilotní projekty MeSH
• předškolní dítě MeSH
• probiotika aplikace a dávkování   MeSH
• prospektivní studie MeSH
• střeva imunologie   metabolismus   účinky léků   MeSH
• střevní sliznice * fyziologie   imunologie   metabolismus   MeSH
• stupeň závažnosti nemoci MeSH
• výsledek terapie MeSH
• Check Tag
• kojenec MeSH
• lidé MeSH
• mužské pohlaví MeSH
• předškolní dítě MeSH
• ženské pohlaví MeSH
• Publikační typ
• práce podpořená grantem MeSH
• srovnávací studie MeSH

Non-typhoid Salmonellae are worldwide spread food-borne pathogens that cause diarrhea in humans and animals. Their multi-drug resistances require alternative ways to combat this enteric pathogen. Mono-colonization of a gnotobiotic piglet gastrointestinal tract with commensal lactobacilli Lactobacillus amylovorus and Lactobacillus mucosae and with probiotic E. coli Nissle 1917 and their interference with S. Typhimurium infection was compared. The impact of bacteria and possible protection against infection with Salmonella were evaluated by clinical signs, bacterial translocation, intestinal histology, mRNA expression of villin, claudin-1, claudin-2, and occludin in the ileum and colon, and local intestinal and systemic levels of inflammatory cytokines IL-8, TNF-α, and IL-10. Both lactobacilli colonized the gastrointestinal tract in approximately 100× lower density compare to E. coli Nissle and S. Typhimurium. Neither L. amylovorus nor L. mucosae suppressed the inflammatory reaction caused by the 24 h infection with S. Typhimurium. In contrast, probiotic E. coli Nissle 1917 was able to suppress clinical signs, histopathological changes, the transcriptions of the proteins, and the inductions of the inflammatory cytokines. Future studies are needed to determine whether prebiotic support of the growth of lactobacilli and multistrain lactobacilli inoculum could show higher protective effects.

• Publikační typ
• časopisecké články MeSH

Increasing numbers of clinical trials and animal experiments have shown that probiotic bacteria are promising tools for allergy prevention. Here, we analyzed the immunomodulatory properties of three selected lactobacillus strains and the impact of their mixture on allergic sensitization to Bet v 1 using a gnotobiotic mouse model. We showed that Lactobacillus (L.) rhamnosus LOCK0900, L. rhamnosus LOCK0908 and L. casei LOCK0919 are recognized via Toll-like receptor 2 (TLR2) and nucleotide-binding oligomerization domain-containing protein 2 (NOD2) receptors and stimulate bone marrow-derived dendritic cells to produce cytokines in species- and strain-dependent manners. Colonization of germ-free (GF) mice with a mixture of all three strains (Lmix) improved the intestinal barrier by strengthening the apical junctional complexes of enterocytes and restoring the structures of microfilaments extending into the terminal web. Mice colonized with Lmix and sensitized to the Bet v 1 allergen showed significantly lower levels of allergen-specific IgE, IgG1 and IgG2a and an elevated total IgA level in the sera and intestinal lavages as well as an increased transforming growth factor (TGF)-β level compared with the sensitized GF mice. Splenocytes and mesenteric lymph node cells from the Lmix-colonized mice showed the significant upregulation of TGF-β after in vitro stimulation with Bet v 1. Our results show that Lmix colonization improved the gut epithelial barrier and reduced allergic sensitization to Bet v 1. Furthermore, these findings were accompanied by the increased production of circulating and secretory IgA and the regulatory cytokine TGF-β. Thus, this mixture of three lactobacillus strains shows potential for use in the prevention of increased gut permeability and the onset of allergies in humans.

• MeSH
• alergie imunologie   prevence a kontrola   MeSH
• antigeny rostlinné toxicita   MeSH
• gnotobiologické modely imunologie   MeSH
• HEK293 buňky MeSH
• imunoglobulin A imunologie   MeSH
• imunoglobulin G imunologie   MeSH
• Lacticaseibacillus rhamnosus imunologie   MeSH
• Lactobacillus casei imunologie   MeSH
• lidé MeSH
• myši inbrední BALB C MeSH
• myši MeSH
• signální adaptorový protein Nod2 imunologie   MeSH
• střevní sliznice imunologie   MeSH
• toll-like receptor 2 imunologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

The aim of this study was to characterize an in vitro modulating effect of three commensal Lactobacillus strains on cellular differentiation of non-transformed crypt-like rat small intestinal cell line IEC-18. IEC-18 was grown on extracellular matrix, with or without presence of Lactobacillus strains. Gene expression of IEC-18 bacterial detection system - such as Toll-like receptors TLR-2, TLR-4, signal adapter MyD88, cytoplasmic NOD2 receptor, inflammatory cytokines IL-18, IL-1beta, chemokine IL-8 and enzyme caspase-1 - was evaluated using real-time PCR. Expression and localization of TLR-2, TLR-4, IL-18 and caspase-1 proteins was demonstrated by Western blotting and immunofluorescent staining. Secretion of IL-18 to apical and basolateral surfaces was assayed by ELISA. Our results suggested that L. casei LOCK0919 accelerated differentiation of IEC-18 by stimulating TLR-2, TLR-4, MyD88, IL-18, caspase-1 mRNAs and proteins. L. casei LOCK0919 increased expression and transfer of villin and beta-catenin from cytoplasm to cell membrane. Presence of L. rhamnosus LOCK0900 resulted in detachment of IEC-18 layer from extracellular matrix leading to induction of IL-1beta, of TLR-2 and IL-8 mRNAs and stimulation of MyD88, caspase-1 and cytosolic receptor NOD2 mRNAs. L. rhamnosus LOCK0908 was not recognized by TLR-2 or TLR-4 receptors. Lactobacilli-IEC-18 crosstalk enhanced immune and barrier mucosal functions.

• MeSH
• beta-katenin biosyntéza   MeSH
• buněčná diferenciace účinky léků   MeSH
• cytokiny biosyntéza   MeSH
• epitelové buňky účinky léků   MeSH
• interleukin-18 biosyntéza   MeSH
• kaspasa 1 biosyntéza   MeSH
• krysa rodu rattus MeSH
• Lacticaseibacillus rhamnosus * MeSH
• Lactobacillus casei * MeSH
• messenger RNA biosyntéza   MeSH
• mikrofilamentové proteiny biosyntéza   MeSH
• probiotika farmakologie   MeSH
• regulace genové exprese účinky léků   MeSH
• střevní sliznice cytologie   účinky léků   MeSH
• subcelulární frakce metabolismus   MeSH
• toll-like receptory biosyntéza   účinky léků   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

• MeSH
• bakteriální adheze fyziologie   imunologie   MeSH
• fermentace MeSH
• hostitelské buněčné faktory MeSH
• Lactobacillus metabolismus   růst a vývoj   MeSH
• povrchové vlastnosti MeSH
• probiotika MeSH
• střevní sliznice mikrobiologie   MeSH

High mobility group box 1 (HMGB1) is a DNA-binding nuclear protein that can be actively secreted by immune cells after different immune stimuli or passively released from cells undergoing necrosis. HMGB1 amplifies inflammation, and its hypersecretion contributes to multiple organ dysfunction syndrome and death. We tested possible immunomodulatory effect of commensal Lactobacillus amylovorus (LA), Lactobacillus mucosae (LM) or probiotic Escherichia coli Nissle 1917 (EcN) in infection of gnotobiotic piglets with Salmonella Typhimurium (ST). Transcription of HMGB1 and Toll-like receptors (TLR) 2, 4, and 9 and receptor for advanced glycation end products (RAGE), TLR4-related molecules (MD-2, CD14, and LBP), and adaptor proteins (MyD88 and TRIF) in the ileum and colon were measured by RT-qPCR. Expression of TLR4 and its related molecules were highly upregulated in the ST-infected intestine, which was suppressed by EcN, but not LA nor LM. In contrast, HMGB1 expression was unaffected by ST infection or commensal/probiotic administration. HMGB1 protein levels in the intestine measured by ELISA were increased in ST-infected piglets, but they were decreased by previous colonization with E. coli Nissle 1917 only. We conclude that the stability of HMGB1 mRNA expression in all piglet groups could show its importance for DNA transcription and physiological cell functions. The presence of HMGB1 protein in the intestinal lumen probably indicates cellular damage.

• MeSH
• Escherichia coli imunologie   MeSH
• gnotobiologické modely imunologie   MeSH
• Lactobacillus acidophilus imunologie   MeSH
• prasata * imunologie   mikrobiologie   MeSH
• probiotika * MeSH
• protein HMGB1 imunologie   MeSH
• Salmonella typhimurium imunologie   MeSH
• signální transdukce imunologie   MeSH
• střeva imunologie   mikrobiologie   MeSH
• toll-like receptor 4 imunologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

Before use in practice, it is necessary to precisely identify and characterize a new probiotic candidate. Eight animal lactobacilli and collection strain Lactobacillus reuteri CCM 3625 were studied from the point of saccharide fermentation profiles, bile salt resistance, antibiogram profiles, and influence of bile on sensitivity to antibiotics. Studied lactobacilli differed in their sugar fermentation ability determined by API 50CHL and their identification based on these profiles did not correspond with molecular-biological one in most cases. Survival of strains Lactobacillus murinus C and L. reuteri KO4b was not affected by presence of bile. The resistance of genus Lactobacillus to vancomycin and quinolones (ofloxacin, ciprofloxacin) was confirmed in all strains tested. This study provides the new information about oxgall (0.5 and 1 %) effect on the lactobacilli antibiotic susceptibility. Antibiotic profiles were not noticeably affected, and both bile concentrations tested had comparable impact on the lactobacilli antibiotic sensitivity. Interesting change was noticed in L. murinus C, where the resistance to cephalosporins was reverted to susceptibility. Similarly, susceptibility of L. reuteri E to ceftazidime arose after incubation in both concentration of bile. After influence of 1 % bile, Lactobacillus mucosae D lost its resistance to gentamicin. On the base of gained outcomes, the best probiotic properties manifested L. reuteri KO4b, Lactobacillus plantarum KG4, and L. reuteri E due to their survival in the presence of bile.

• MeSH
• antibakteriální látky farmakologie   MeSH
• kozy MeSH
• Lactobacillus účinky léků   izolace a purifikace   MeSH
• ovce MeSH
• probiotika analýza   MeSH
• tolerance léku MeSH
• žaludek mikrobiologie   MeSH
• žlučové kyseliny a soli farmakologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

This study was undertaken to investigate the starter and probiotic potential of lactic acid bacteria isolated from dromedarian camel's milk using both culture-dependent and -independent approaches and metataxonomic analysis. Strains of lactic acid bacteria recovered were examined in vitro for tolerance to gastric acidity, bile, and lysozyme. Bile salt hydrolysis, serum cholesterol-lowering, oxalate degradation, proteolytic activity, exopolysaccharide production, and cell surface characteristics necessary for colonizing intestinal mucosa were also evaluated. A single strain of the species, Lactobacillus fermentum named NPL280, was selected through multivariate analysis as it harbored potential probiotic advantages and fulfilled safety criteria. The strain assimilated cholesterol, degraded oxalate, produced exopolysaccharides, and proved to be a proficient alternate yogurt starter with good viability in stored bio-yogurt. A sensorial analysis of the prepared bio-yogurt was also found to be exemplary. We conclude that the indigenous L. fermentum strain NPL280 has the desired traits of a starter and adjunct probiotic culture for dairy products.

• MeSH
• jogurt mikrobiologie   MeSH
• Limosilactobacillus fermentum * metabolismus   MeSH
• mikrobiota fyziologie   MeSH
• mléko * mikrobiologie   MeSH
• probiotika * klasifikace   izolace a purifikace   metabolismus   MeSH
• velbloudi MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Pákistán MeSH

Several biochemical and molecular methods were used for discrimination of four Lactobacillus reuteri strains isolated from goatling and lamb stomach mucosa. Internal transcribed spacer (ITS)-PCR method and protein analysis by SDS-PAGE and MALDI-TOF showed to be suitable for strain discrimination whereas ITS-PCR/RFLP and enterobacterial repetitive intergenic consensus (ERIC)-PCR were not strain specific. The used methods differentiated tested strains into distinct groups; however, the location of strains in groups varied. Consistency in results was observed in the case of L. reuteri E and L. reuteri KO4m that were clustered into the same groups using all techniques, except of MALDI-TOF MS. The last one grouped goatling strains and lamb isolate into separate clusters. All investigated methods, except of ITS-PCR/RFLP and ERIC-PCR, were assessed as appropriate for distinguishing of L. reuteri strains.

• MeSH
• bakteriální proteiny analýza   MeSH
• bakteriologické techniky metody   MeSH
• DNA bakterií chemie   genetika   MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• kozy MeSH
• Limosilactobacillus reuteri klasifikace   genetika   izolace a purifikace   fyziologie   MeSH
• mezerníky ribozomální DNA chemie   genetika   MeSH
• ovce MeSH
• polymerázová řetězová reakce MeSH
• reprodukovatelnost výsledků MeSH
• shluková analýza MeSH
• spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
• žaludeční sliznice mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• srovnávací studie MeSH