functionalized surface
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Práca poskytuje prehľad najnovších poznatkov o funkcii Eustachovej trubice v súvislosti s povrchovoaktívnym materiálom – surfaktantom, ktorý vystieľa jej povrch. Zdôrazňuje sa vzťah medzi poškodením surfaktantového systému a dysfunkciou Eustachovej trubice s následným rozvojom zápalu stredného ucha. Diskutujú sa výsledky štúdií, ktoré naznačujú možné trendy vo využití exogénneho surfaktantu v liečbe otitis media.
The authors give a review of the current knowledge on the function of the Eustachian tube (ET) regarding the surface-active material - surfactant – present at its mucosal surface. Detergent-like surface properties of ET linning fluid act to support ET function by facilitating the intermittent opening and closure of its lumen. Specialized cells in ET epithelium express surfactant specific proteins SP-A and SP-D and these proteins are secreted into the ET lumen. They play a role in local immune mechanisms by clearance of pathogenes and acting as immunomodulators. Dysfunction of the local mucosal immunity in the ET may predispose infants to recurrent otitis media. Administration of surfactant improves mechanical properties of ET and restores the impaired local immunity. The authors further discuss the results of the experimental studies in order to indicate the future trends in the use of exogenous surfactant for the treatment of otitis media.
In this study, we investigate the preparation of surface pattern of functional groups on poly(lactide) (PLA) surfaces through the controlled deposition of core-shell self-assemblies based on functionalized PLA-b-PEO amphiphilic block copolymers from selective solvents. Through grafting RGDS peptide onto the functionalized copolymer surface, the presented approach enables to prepare PLA surfaces with random and clustered spatial distribution of adhesive motifs. The proposed topography of the adhesion motif was proved by atomic force microscopy techniques using biotin-tagged RGDS peptide grafted on the surface and streptavidin-modified gold nanospheres which bind the tagged RGDS peptides as a contrast agent. The cell culture study under static and dynamic conditions with MG63 osteosarcoma cell line showed that the clustered distribution of RGDS peptides provided more efficient initial cell attachment and spreading, and resistance to cell detachment under dynamic culture compared to randomly distributed RGDS motif when with the same average RGDS peptide concentration.
- MeSH
- biomimetika MeSH
- buněčná adheze účinky léků MeSH
- kovové nanočástice MeSH
- laktáty chemie MeSH
- lidé MeSH
- mikroskopie atomárních sil MeSH
- nádorové buněčné linie MeSH
- nanostruktury chemie MeSH
- oligopeptidy MeSH
- polyethylenglykoly chemie MeSH
- povrchové vlastnosti MeSH
- streptavidin chemie MeSH
- vazba proteinů MeSH
- zlato MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Článek pojednává o nejčastějších možnostech využití povrchové elektromyografie. Shrnuje především biomechanické a kineziologické aplikace: pozorování timingu (načasování svalového zapojení), velikosti aktivace a únavy svalu. Sledování funkce svalů umožňuje analýzu lidského pohybu. Je však nutné respektovat vliv dalších faktorů, které se na vzniku signálu podílejí. Studie upozorňuje na omezené možnosti i perspektivy využití v souvislosti se sledováním a hodnocením neuromuskulárních poruch.
The paper deals with the most frequent possibilities of application of surface electromyography. It summarizes above all biomechanical and kinesiological applications: observation of the timing of muscle involvement, magnitude of muscle activation and fatigue. Investigation of muscular function makes analysis of human motion possible. It is however necessary to respect the influence of further factors which participate in the development of the signal. The investigation draws attention to the limited possibilities and perspectives of application in conjunction with the investigation and evaluation of neuromuscular disorders.
- MeSH
- elektromyografie metody využití MeSH
- lidé MeSH
- svalová únava fyziologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
The non-specific binding of non-target species to functionalized surfaces of biosensors continues to be challenge for biosensing in real-world media. Three different low-fouling and functionalizable surface platforms were employed to study the effect of functionalization on fouling resistance from several types of undiluted media including blood plasma and food media. The surface platforms investigated in this work included two polymer brushes: hydroxy-functional poly(2-hydroxyethyl methacrylate) (pHEMA) and carboxy-functional poly(carboxybetaine acrylamide) (pCBAA), and a standard OEG-based carboxy-functional alkanethiolate self-assembled monolayer (AT-SAM). The wet and dry polymer brushes were analyzed by AFM, ellipsometry, FT-IRRAS, and surface plasmon resonance (SPR). The surfaces were functionalized by the covalent attachment of antibodies, streptavidin, and oligonucleotides and the binding and biorecognition characteristics of the coatings were compared. We found that functionalization did not substantially affect the ultra-low fouling properties of pCBAA (plasma fouling of ~20 ng/cm(2)), a finding in contrast with pHEMA that completely lost its resistance to fouling after the activation of hydroxyl groups. Blocking a functionalized AT-SAM covalently with BSA decreased fouling down to the level comparable to unblocked pCBAA. However, the biorecognition capability of blocked functionalized AT-SAM was poor in comparison with functionalized pCBAA. Limits of detection of Escherichia coli O157:H7 in undiluted milk were determined to be 6×10(4), 8×10(5), and 6×10(5) cells/ml for pCBAA, pHEMA, and AT-SAM-blocked, respectively. Effect of analyte size on biorecognition activity of functionalized coatings was investigated and it was shown that the best performance in terms of overall fouling resistance and biorecognition capability is provided by pCBAA.
- MeSH
- adsorpce MeSH
- akrylamidy chemie MeSH
- Escherichia coli izolace a purifikace MeSH
- limita detekce MeSH
- mléko mikrobiologie MeSH
- polyhydroxyethylmethakrylát chemie MeSH
- polymery chemie MeSH
- povrchová plasmonová rezonance metody MeSH
- povrchové vlastnosti MeSH
- sulfhydrylové sloučeniny chemie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
The field of material surface modification with the aim of biomaterial construction involves several approaches of treatments that allow the preparation of materials, which positively influence adhesion of cells and their proliferation and thus aid and improve tissue formation. Modified materials have a surface composition and morphology intended to interact with biological systems and cellular functions. Not only surface chemistry has an effect on material biological response, surface structures of different morphology can be constructed to guide a desirable biological outcome. Nano-patterned material surfaces have been tested with the aim of how surface geometry and physical properties on a micro- and nano-scale can affect cellular response and influence cell adhesion and proliferation. Biological functionality of solid state substrates was significantly improved by the irradiation of material with plasma discharge or laser treatment. Commonly used "artificial" polymers (e.g. polyethylene (PE), polystyrene (PS), polytetrafluoroethylene (PTFE), polyethylene terephthalate (PET), polyethylene naphthalate (PEN)) and biopolymers (e.g. Poly-l-Lactic acid (PLLA), polymethylpentene (PMP)) were treated with aim of biocompatibility improvement. The treatment of polymer/biopolymer substrates leads to formation of ripple or wrinkle-like structures, supported also with heat treatment or other subsequent surface processing. Several types of chemically different substances (e.g. metal or carbon nano-particles, proteins) were grafted onto material surfaces or built into material structures by different processes. Surface physico-chemical properties (e.g. chemistry, charge, morphology, wettability, electrical conductivity, optical and mechanical properties) of treated surfaces were determined. The enhancement of adhesion and proliferation of cells on modified substrates was investigated in vitro. Bactericidal action of noble metal nano-particles (e.g. Au, Ag) on polymers was characterized. The influence of metal nano-particle grafting by using metal nano-particle suspension prepared by "green" methods was determined. Micro- and nano-patterned surfaces can be constructed as tissue scaffolds with specific functions regarding cell adhesion and proliferation or potential biosensor applications.
- MeSH
- biomedicínský výzkum MeSH
- lidé MeSH
- poranění prstů ruky MeSH
- prsty ruky chirurgie MeSH
- transplantace metody MeSH
- Check Tag
- lidé MeSH
... ZACHOWSKI) 3 -- Protein-lipid interactions at membrane surfaces (A. ... ... WATTS) 23 -- The super-ordered fluid: A domain model of cell surface membranes -- (M. ... ... PHYSICOCHEMICAL ASPECTS -- Cell surface electrostatics and electrokinetics -- (A. VOIGT & E. ... ... FUNCTIONAL ASPECTS -- The influence of membrane electric field on cellular functions -- (R. ... ... HOEKSTRA) 237 -- VIII -- Cell contact with solid surfaces (D. ...
Springer series in biophysics ; Vol. 5
viii, 366 s. : il., tab. ; 24 cm
- Konspekt
- Biochemie. Molekulární biologie. Biofyzika
- NLK Obory
- fyzika, biofyzika
- cytologie, klinická cytologie
- NLK Publikační typ
- kolektivní monografie
