Chicks Dotaz Zobrazit nápovědu
- MeSH
- komplement MeSH
- kur domácí MeSH
- Poxviridae MeSH
- vakcinace MeSH
- virové nemoci MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- MeSH
- komplement MeSH
- kur domácí MeSH
- Poxviridae MeSH
- vakcinace MeSH
- virové nemoci MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
Background: Progesterone is the source of the steroid general anesthetic Alfaxalone, which became commercially available in the 1970s when combined with alfadolone, another steroid anesthetic.Objective: the goal of the present research was to discover and assess the sub-anesthetic effects of Alfaxalone: the antinociceptive and anti-inflammatory properties in broiler chicks. Methods: One hundred and three Ross broiler chicks (80-100 g, 7-8 days old) were used. The electric stimulation test, hot water test (HWT), and formalin test were performed on broiler chicks to assess the antinociceptive and anti-inflammatory properties of Alfaxalone. The up-and-down method that described by Dixon was used to determine the median effective antinociceptive dose.Results: The median effective antinociceptive doses (ED50s) of Alfaxalone by electric stimulation test and hot water test were 0.94 and 0.65 mg/kg intraperitoneally, respectively. Alfaxalone, at doses of 1, 2, and 4 mg/kg intraperitoneally, induced an antinociceptive effect in the electric stimulation test and HWT as well as antinociceptive and anti-inflammatory effects during the formalin test in the chicks. The antinociceptive effect was displayed 15 min after treatment and the maximum effect was observed 30 min after injection.Conclusion: The results showed that Alfaxalone had antinociceptive and anti-inflammatory effects, with a stronger antinociceptive effect in the electric stimulation test than in the thermal stimulation test, which was illustrated by shorter antinociceptive time.
- Klíčová slova
- alfaxalon,
- MeSH
- analgetika aplikace a dávkování farmakologie MeSH
- antiflogistika aplikace a dávkování farmakologie MeSH
- elektrická stimulace MeSH
- fyzikální stimulace metody MeSH
- injekce intraperitoneální MeSH
- kur domácí MeSH
- modely u zvířat MeSH
- pregnandiony * aplikace a dávkování farmakologie MeSH
- Publikační typ
- práce podpořená grantem MeSH
This study aimed to investigate the concentration of pregabalin in the plasma of chicks to determine its pharmacokinetic parameters. Pregabalin (300 mg/kg) was administered orally to 42 clinically healthy Ross chicks as part of a randomized controlled study. Blood samples were collected from the jugular vein at 0.5, 1, 2, 4, 8, and 24 h after drug administration from six chicks per each time. The concentrations of pregabalin in the plasma samples were determined using a quantitative HPLC assay, and pharmacokinetic parameters were calculated using the PKSolver program. Pharmacokinetic parameters were determined using a noncom-partmental model. The concentrations of pregabalin were 133.80 ± 2.35, 183.20 ± 3.91, 295.60 ± 2.82, 248.40 ± 7.60, 219.00 ± 2.72 and 154.00 ± 5.50 μg/ml at the times 0.5, 1, 2, 4, 8, and 24 h respectively. The pharmacokinetics parameters were t1/2β 29 h, Tmax 2 h, Cmax 295 μg, Kel 0.023 h-1, MRT 43h, Vd 1.127 L/h/kg, Cl 0.026 L/h/kg and AUC0-∞ 11420.31 μg.h/ml. This study concluded that pregabalin has a long elimination half-life and poor clearance from the animal body, which is reflected in the prolonged impact of its action.
