The presented work describes the development of a simple, fast and effective on-line SPE-UHPLC-UV/vis method using fused core particle columns for extraction, separation and quantitative analysis of the nine illegal dyes, most frequently found in chilli-containing spices. The red dyes Sudan I-IV, Sudan Red 7B, Sudan Red G, Sudan Orange G, Para Red, and Methyl Red were separated and analyzed in less than 9 min without labor-consuming pretreatment procedure. The chromatographic separation was performed on Ascentis Express RP-Amide column with gradient elution using mixture of acetonitrile and water, as a mobile phase at a flow rate of 1.0 mL min(-1) and 55°C of temperature. As SPE sorbent for cleanup and pre-concentration of illegal dyes short guard fused core column Ascentis Express F5 was used. The applicability of proposed method was proven for three different chilli-containing commercial samples. Recoveries for all compounds were between 90% and 108% and relative standard deviation ranged from 1% to 4% for within- and from 2% to 6% for between-day. Limits of detection showed lower values than required by European Union regulations and were in the range of 3.3-10.3 µg L(-1) for standard solutions, 5.6-235.6 µg kg(-1) for chilli-containing spices.
- MeSH
- Food Analysis methods MeSH
- Azo Compounds chemistry MeSH
- Coloring Agents chemistry isolation & purification MeSH
- Capsicum chemistry MeSH
- Solid Phase Extraction methods MeSH
- Indicators and Reagents chemistry MeSH
- Spices analysis MeSH
- Naphthols chemistry MeSH
- Online Systems MeSH
- Food Coloring Agents chemistry MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
V průběhu posledních cca dvou let probíhá v celoevropském měřítku i v ČR diskuze o stále siřeji používané „on-line" hemodiafiltraci (HDF), a to zejména se zaměřením na význam velikosti objemu substitučního roztoku. Cílem tohoto článku je poskytnout těmto diskuzím odpovídající odborný rámec. Na základě definiční práce skupiny EUDIAL, věnující se této problematice pod hlavičkou ERA-EDTA, je uveden aparát pro hodnocení účinnosti této konvektivní metody. Dále jsou podrobněji probrány tři základní práce, které přišly s konceptem tzv. vysokoobjemové HDF a zabývaly se dopadem objemu substitučního roztoku na morbiditu a mortalitu léčených pacientů. Tato analýza je doplněna daty z několika nejnovějších prací o HDF, orientovaných na specifické přednosti a úskalí HDF. Zdůrazněn je i kritický význam čistoty vody se stoupajícími směňovanými objemy
There has been an intense discussion on ever-growing use of the on-line haemodiafiltration (HDF), both in our country and Europe- wide, focused namely on importance of substitution fluid volume used. The aim of this article is to provide corresponding expert frame for that discussion. Based on the definition work of the EUDIAL group active in this field under the umbrella of the ERA-EDTA, the tools to assess efficacy of this convective method are outlined. Three recent fundamental publications, which came with the concept of so- called high-volume HDF are analyzed in detail, namely their conclusions regarding the impact of substitution volume on morbidity and mortality of treated patients. This analysis is complemented by data from a few most recent publications which discuss specific pros and cons of HDF. Stressed is also critical importance of water purity with increasing substitution volumes.
- Keywords
- konvektivní objem, substituční objem, ultračistý substituční roztok, studie CONTRAST, studie ESHOL, turecká studie OL-HDF,
- MeSH
- Kidney Failure, Chronic therapy MeSH
- Dialysis Solutions administration & dosage MeSH
- Hemodiafiltration * methods mortality adverse effects MeSH
- Convection MeSH
- Water Quality MeSH
- Humans MeSH
- Randomized Controlled Trials as Topic MeSH
- Models, Statistical MeSH
- Terminology as Topic MeSH
- Check Tag
- Humans MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
sv.
- MeSH
- Epidemiologic Methods MeSH
- Epidemiology MeSH
- Data Collection MeSH
- Publication type
- Periodical MeSH
- Conspectus
- Veřejné zdraví a hygiena
- NML Fields
- epidemiologie
- statistika, zdravotnická statistika
- MeSH
- Epidemiologic Research Design MeSH
- Information Systems utilization MeSH
- Internet utilization MeSH
- Medical Oncology methods trends MeSH
- Humans MeSH
- Neoplasms epidemiology MeSH
- Online Systems utilization MeSH
- Registries standards statistics & numerical data MeSH
- Data Collection methods trends MeSH
- Government Agencies utilization MeSH
- Check Tag
- Humans MeSH
- Publication type
- News MeSH
- Geographicals
- Czech Republic MeSH
- MeSH
- Electronic Data Processing utilization MeSH
- Disease Notification methods standards statistics & numerical data MeSH
- Internet utilization MeSH
- Medical Oncology methods organization & administration trends MeSH
- Humans MeSH
- Neoplasms epidemiology MeSH
- Online Systems utilization MeSH
- Computer-Assisted Instruction methods utilization MeSH
- Registries standards statistics & numerical data MeSH
- Data Collection methods trends utilization MeSH
- Check Tag
- Humans MeSH
- Geographicals
- Czech Republic MeSH
In this work, an on-line SPE-HPLC method with spectrophotometric detection was developed for the determination of coumarins in complex samples. For the on-line cleanup of samples, a molecularly imprinted polymer was packed into the column cartridge and coupled directly with HPLC (MISPE-HPLC) using a column switching system. The separation of coumarins was performed on a C18 core-shell column (100×4.6mm, 5μm) with a mobile phase consisting of 0.3% acetic acid/acetonitrile with gradient elution at a flow-rate of 1mLmin-1. The total time of the whole analytical run, including the extraction step, was 13.25min. The on-line MISPE-HPLC method was optimized and validated. The results showed good linearity (0.10-100μgmL-1) with correlation coefficients higher than 0.99. The LOD values were from 0.03 to 0.15μgmL-1. The proposed method was successfully applied for analysis of real samples (Cassia cinnamon, chamomile tea, and Tokaj specialty wines) and obtained recoveries varied from 78.7% to 112.2% with an RSD less than 9%.
