Parapharyngeal abscess in an infant is a very rare condition. We present the case of a 4-month-old girl with large masses on the neck's left side. Computed tomography showed an extensive parapharyngeal abscess. Left tonsillectomy was performed under general anesthesia from a transoral approach, followed by an incision and evacuation of the abscess from the parapharyngeal space. Microbiological analysis identified a massive occurrence of Streptococcus intermedius.

• MeSH
• absces * mikrobiologie   chirurgie   MeSH
• kojenec MeSH
• lidé MeSH
• nemoci faryngu mikrobiologie   chirurgie   MeSH
• počítačová rentgenová tomografie MeSH
• spatium parapharyngeum * chirurgie   diagnostické zobrazování   mikrobiologie   MeSH
• Streptococcus intermedius izolace a purifikace   MeSH
• streptokokové infekce * mikrobiologie   chirurgie   MeSH
• tonzilektomie MeSH
• Check Tag
• kojenec MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH

Methicillin-resistant Staphylococcus aureus (MRSA) is a dangerous pathogen occurring not only in hospitals but also in foodstuff. Currently, discussions on the issue of the increasing resistance, and timely and rapid diagnostic of resistance strains have become more frequent and sought. Therefore, the aim of this study was to design an effective platform for DNA isolation from different species of microorganisms as well as the amplification of mecA gene that encodes the resistance to β-lactam antibiotic formation and is contained in MRSA. For this purpose, we fabricated 3D-printed chip that was suitable for bacterial cultivation, DNA isolation, PCR, and detection of amplified gene using gold nanoparticle (AuNP) probes as an indicator of MRSA. Confirmation of the MRSA presence in the samples was based on a specific interaction between mecA gene with the AuNP probes and a colorimetric detection, which utilized the noncross-linking aggregation phenomenon of DNA-functionalized AuNPs. To test the whole system, we analyzed several real refractive indexes, in which two of them were positively scanned to find the presence of mecA gene. The aggregation of AuNP probes were reflected by 75% decrease of absorbance (λ = 530 nm) and change in AuNPs size from 3 ± 0.05 to 4 ± 0.05 nm (n = 5). We provide the one-step identification of mecA gene using the unique platform that employs the rapid, low-cost, and easy-to-use colorimetric method for MRSA detection in various samples.

• MeSH
• absces mikrobiologie   MeSH
• bakteriální proteiny genetika   MeSH
• design vybavení MeSH
• DNA bakterií analýza   genetika   MeSH
• kovové nanočástice chemie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• methicilin rezistentní Staphylococcus aureus genetika   izolace a purifikace   MeSH
• molekulární typizace MeSH
• sekvenční analýza hybridizací s uspořádaným souborem oligonukleotidů přístrojové vybavení   metody   MeSH
• stafylokokové infekce mikrobiologie   MeSH
• techniky amplifikace nukleových kyselin MeSH
• zlato chemie   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Concha bullosa (CB) is among the most common anatomic variations of sinonasal anatomy. Although usually asymptomatic, CB can occasionally cause nasal obstruction or headache. Obstructions within the mucociliary transport system can develop into a mucocele or mucopyocele. A 48-year-old female, with a history of progressive headache and nasal obstruction, was referred to our department. Paranasal sinus tomography revealed a nasal mass in the left nasal cavity resembling a mucopyocele in the middle turbinate. Under general anesthesia, the purulent material was aspirated, and the lateral part of the left turbinate was resected. Mucopyoceles are common within the paranasal sinuses, but uncommon with CB; thus, they should be considered in patients with a large hyperemic nasal mass.

• MeSH
• absces komplikace   diagnóza   mikrobiologie   terapie   MeSH
• antibakteriální látky terapeutické užití   MeSH
• endoskopie MeSH
• lidé středního věku MeSH
• lidé MeSH
• mukokéla komplikace   diagnóza   mikrobiologie   terapie   MeSH
• nemoci nosu komplikace   diagnóza   terapie   MeSH
• nosní obstrukce diagnóza   etiologie   terapie   MeSH
• nosní skořepy abnormality   MeSH
• počítačová rentgenová tomografie MeSH
• stafylokokové infekce komplikace   diagnóza   terapie   MeSH
• Staphylococcus aureus MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH

BACKGROUND: The aim of this study was to review the bacteriology of deep neck infections (DNI) and identify the factors that influence the incidence of causative bacteria. METHODS: A retrospective analysis of 634 patients with DNI was performed. Statistical analysis was used to compare the incidence of common pathogens in various conditions such as age of the patients, aetiology and associated diabetes mellitus. RESULTS: Bacteria were isolated in 514 cultures (81%). Aerobic bacteria were isolated from 246 cultures (39%) and anaerobic bacteria from 61 cultures (10%). Both aerobic and anaerobic bacteria were isolated from 207 cultures (32%). The most common aerobic bacteria were Streptococcus pyogenes (41%) and Staphylococcus aureus (32%). The most common anaerobic bacteria were Peptostreptococcus species (28%), followed by Prevotela species (8%) and Proprionibacterium species (7%). The incidence of anaerobic bacteria was higher in adults, in patients with infections of dental origin and in non-diabetic patients. CONCLUSIONS: The bacteriology of DNI is polymicrobial, including both aerobic and anaerobic bacteria. The incidence of anaerobic bacteria is higher in adults, in patients with infections of dental origin and in non-diabetic patients.

• MeSH
• absces epidemiologie   mikrobiologie   prevence a kontrola   MeSH
• aerobní bakterie izolace a purifikace   MeSH
• anaerobní bakterie izolace a purifikace   MeSH
• diabetes mellitus * MeSH
• dítě MeSH
• dospělí MeSH
• incidence MeSH
• kojenec MeSH
• krk * MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• předškolní dítě MeSH
• retrospektivní studie MeSH
• rizikové faktory MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• stafylokokové infekce epidemiologie   mikrobiologie   prevence a kontrola   MeSH
• Staphylococcus aureus izolace a purifikace   MeSH
• Streptococcus pyogenes izolace a purifikace   MeSH
• streptokokové infekce epidemiologie   mikrobiologie   prevence a kontrola   MeSH
• věkové faktory MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• kojenec MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• předškolní dítě MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH

Streptococcus suis je významný patogen prasat s celosvětovým výskytem a možným přenosem infekce na člověka. Hlášeny byly sporadické výskyty onemocnění lidí po celém světě, včetně Evropy. U člověka může vést k rozvoji řady závažných stavů. Mezi nejčastější patří meningitida a sepse. Prezentovaná kazuistika popisuje rozvoj rozsáhlé klinické manifestace infekce od sepse, spondylodiscitidy, paravertebrálních abscesů až k osteomyelitidě a poškození sluchu. Onemocnění ve svém důsledku vedlo k několikaměsíční hospitalizaci a těžké invalidizaci pacienta.

Streptococcus suis is regarded to be a significant pathogen in swine with global prevalence and possible transmission of infection to humans. Sporadic cases of infection have been reported worldwide including Europe. Such infection in humans leads to development of various serious conditions, including, most frequently, meningitis and sepsis. The presented case report describes extensive clinical presentation of the infection, ranging from sepsis, spondylodiscitis and abscess formation in paravertebral muscles to osteomyelitis and hearing impairment. In our case, the disease led to several months of hospitalization and severe disability. Key words: Streptococcus suis – Czech Republic – spondylodiscitis – abscess The authors declare they have no potential conflicts of interest concerning drugs, products, or services used in the study. The Editorial Board declares that the manu­script met the ICMJE “uniform requirements” for biomedical papers.

• MeSH
• absces * diagnóza   farmakoterapie   mikrobiologie   MeSH
• antibakteriální látky MeSH
• discitida * diagnóza   farmakoterapie   mikrobiologie   MeSH
• hluchota etiologie   komplikace   MeSH
• kyčelní kloub chirurgie   mikrobiologie   patologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• magnetická rezonanční tomografie MeSH
• meningitida bakteriální komplikace   MeSH
• mozek radiografie   MeSH
• nemoci páteře diagnóza   farmakoterapie   mikrobiologie   MeSH
• nemoci prasat přenos   MeSH
• osteomyelitida diagnóza   chirurgie   komplikace   MeSH
• péče o pacienty v kritickém stavu MeSH
• polymerázová řetězová reakce MeSH
• progrese nemoci MeSH
• sepse komplikace   MeSH
• sinusitida etiologie   komplikace   MeSH
• Streptococcus suis * izolace a purifikace   patogenita   MeSH
• streptokokové infekce diagnóza   farmakoterapie   komplikace   MeSH
• zoonózy MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• kazuistiky MeSH
• práce podpořená grantem MeSH

PURPOSE OF THE STUDY: Leukocyte esterase is an enzyme in neutrophils from which it is released into exudate; its detection by colorimetric test strips indicates the presence of neutrophils. This is a rapid method to find whether exudate is of infectious or non-infectious aetiology. The aim of the study was to determine the sensitivity and specificity of leukocyte esterase testing with use of AUTION Sticks (Arkray) for examination of exudates obtained in inflammatory diseases of the skeletal system. MATERIAL AND METHODS: Exudates associated with skeletal system diseases were collected from 45 patients in the period from July 1st to December 31 st , 2012. Aspirates obtained under sterile conditions were examined for leukocyte esterase; cytological and microbiological examinations were also carried out. For the detection of leukocyte esterase, a drop of aspirate was placed on the reagent zone of a test strip and the resulting colour reaction was read after 90 minutes. Changes in colour were compared with a reference strip provided by the manufacturer. The results were assessed on a five-shade scale as follows: 0, no colour change; 1 to 4, gradual change from light pink to deep purple. The results were compared with those of cytological and microbiological examinations. Shade 4 on the strip corresponded to a positive cytological finding of bacterial infection, and shades 3 and 4 correlated with a positive microbial finding. The sensitivity and specificity of leukocyte esterase testing were statistically evaluated for both comparisons. RESULTS: Based on the results of cytological and microbiological examinations, an infectious aetiology of exudate was diagnosed in 21 (44.4%) and non-infectious aetiology in 24 (63.6%) patients. With leukocyte esterase reagent strips when shade 4 was taken as a positive result, the sensitivity and specificity of examination was assessed as 0.6190 and 0.9583, respectively. When taking both shade 3 and shade 4 for a positive result, sensitivity and specificity were 0.8571 and 0.8750, respectively. Shades 0 and 1 corresponded to the number of leukocytes in exudate that was no higher than 2 x 10⁹/ml. DISCUSSION: The detection of leukocyte esterase is a quick and easy examination. It is useful for readily excluding or confirming an infectious aetiology of exudate and can, to some extent, substitute a cytological examination. It can also help to make a quick decision whether one- or two-stage joint reimplantation should be performed and thus eliminate the need of intra-operative histological examination of frozen tissue samples. A drawback of the method was that exudate samples contaminated with blood interfered with an assessment of colour shades. However, this can be avoided by centrifugation of the sample and use of a supernatant free from erythrocytes. CONCLUSIONS: Diagnosing infectious aetiology of joint exudate or exudate from an abscess using leukocyte esterase reagent strips appears, according to our results, to be a promising, semi-quantitative method with high specificity and sensitivity which is rapid, simple and affordable. It can be useful particularly in out-patient institutions for a quick diagnosis of arthritis; intraoperatively, it can serve as an additional method to other exudate examinations.

• MeSH
• absces diagnóza   mikrobiologie   MeSH
• artritida diagnóza   mikrobiologie   MeSH
• bakteriální infekce diagnóza   MeSH
• cytodiagnostika MeSH
• exsudáty a transsudáty enzymologie   MeSH
• karboxylesterhydrolasy analýza   MeSH
• lidé MeSH
• nemoci kostí diagnóza   mikrobiologie   MeSH
• neutrofily enzymologie   MeSH
• reagenční papírky * MeSH
• senzitivita a specificita MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• srovnávací studie MeSH

Thyroid abscess is a relatively rare yet dramatic condition of the thyroid gland requiring immediate therapeutic intervention. Traditionally, more or less aggressive surgical approaches and administration of broad-spectrum antibiotics have been used. Clinically less severe disease allows non-surgical treatment as well. The case report describes successful treatment of a large abscess of iatrogenic origin after biopsy of a cystic cavity. A combination of broad-spectrum antibiotics was used based on culture results, administered both orally and by repeated application directly into the abscess cavity using an ultrasound-guided fine-needle approach. Concurrent repeated evacuation of the cavity replaced drainage. Ultimately, a small residual cavity with sterile contents was managed by sclerotization with absolute alcohol. Clinical condition permitting, thyroid abscess may be successfully treated by repeated application of a targeted antibiotic, using a fine needle and ultrasound guidance, directly into the abscess cavity, with repeated evacuation replacing drainage.

• MeSH
• absces farmakoterapie   etiologie   mikrobiologie   terapie   ultrasonografie   MeSH
• antibakteriální látky aplikace a dávkování   terapeutické užití   MeSH
• ciprofloxacin aplikace a dávkování   terapeutické užití   MeSH
• cysty patologie   MeSH
• dospělí MeSH
• Enterobacter * izolace a purifikace   MeSH
• enterobakteriální infekce farmakoterapie   etiologie   mikrobiologie   terapie   ultrasonografie   MeSH
• ethanol aplikace a dávkování   terapeutické užití   MeSH
• gentamiciny aplikace a dávkování   terapeutické užití   MeSH
• infekce v ráně farmakoterapie   etiologie   mikrobiologie   terapie   ultrasonografie   MeSH
• intervenční ultrasonografie * MeSH
• kombinovaná terapie MeSH
• lidé MeSH
• nemoci štítné žlázy farmakoterapie   etiologie   mikrobiologie   terapie   ultrasonografie   MeSH
• skleroterapie * MeSH
• sklerotizující roztoky aplikace a dávkování   terapeutické užití   MeSH
• tenkojehlová biopsie škodlivé účinky   MeSH
• tracheotomie MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• přehledy MeSH

• MeSH
• absces farmakoterapie   mikrobiologie   MeSH
• antibakteriální látky aplikace a dávkování   MeSH
• cholangitida farmakoterapie   mikrobiologie   MeSH
• hospitalizovaní pacienti MeSH
• infekce získané v komunitě farmakoterapie   mikrobiologie   MeSH
• kombinovaná terapie MeSH
• lidé MeSH
• pneumonie farmakoterapie   mikrobiologie   MeSH
• pyelonefritida farmakoterapie   mikrobiologie   MeSH
• senioři MeSH
• sepse farmakoterapie   mikrobiologie   MeSH
• Check Tag
• lidé MeSH
• senioři MeSH
• Publikační typ
• novinové články MeSH

Staphylococcus aureus ssp anaerobius strain S10 was isolated from an outbreak of sheep abscess disease. Sequence of the catalase gene of this strain showed 99% identity to the catalase gene (katB) sequence of the reference strain (S. aureus ssp. anaerobius strain MVF213) with mismatching of three base pairs. An important substitution located 1036 nucleotides upstream of the initiation codon from "C" in katB to "T" in the catalase gene of strain S10 originated a stop codon. The deduced protein (345 amino acids) is 105 amino acids shorter than that of katB. Partial sequence of the catalase gene of other 8 local isolates in addition to another reference strain (DSM 20714/ATCC 35844) revealed the same mutations in all local (African) strains, whereas the sequence of the reference (European) strain was typical to that of katB. Sequence of the catalase gene of S. aureus ssp. anaerobius strain S10 was deposited in GenBank under accession no. EU281993.

• MeSH
• absces epidemiologie   mikrobiologie   veterinární   MeSH
• bakteriální proteiny genetika   MeSH
• DNA bakterií genetika   chemie   MeSH
• epidemický výskyt choroby MeSH
• financování organizované MeSH
• katalasa genetika   MeSH
• molekulární sekvence - údaje MeSH
• nemoci ovcí epidemiologie   mikrobiologie   MeSH
• nesmyslný kodon MeSH
• ovce MeSH
• polymorfismus genetický MeSH
• sekvenční analýza DNA MeSH
• sekvenční homologie nukleových kyselin MeSH
• stafylokokové infekce epidemiologie   mikrobiologie   veterinární   MeSH
• Staphylococcus aureus enzymologie   izolace a purifikace   klasifikace   MeSH
• substituce aminokyselin genetika   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH

• MeSH
• absces farmakoterapie   mikrobiologie   MeSH
• Actinomyces účinky léků   izolace a purifikace   MeSH
• aktinomykóza farmakoterapie   mikrobiologie   MeSH
• antibakteriální látky farmakologie   terapeutické užití   MeSH
• dospělí MeSH
• lidé MeSH
• mikrobiální testy citlivosti MeSH
• mladiství MeSH
• molekulární sekvence - údaje MeSH
• nemoci anu farmakoterapie   mikrobiologie   MeSH
• nemoci mužských pohlavních orgánů farmakoterapie   mikrobiologie   MeSH
• senioři MeSH
• skrotum mikrobiologie   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• dopisy MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH