Úvod: Kapslová endoskopie patří ke standardním diagnostickým metodám vyšetření tenkého střeva. Hlavní výhodou je neinvazivita, a to jak v klinické praxi, tak v experimentu. Cíl: Cílem pilotního projektu bylo vypracovat metodiku vyšetření kapslovou endoskopií s boční optikou u experimentálního prasete a tuto metodiku ověřit na zdravých zvířatech. Metodika: Do studie bylo zařazeno celkem šest experimentálních prasat (Sus scrofa f. domestica) o průměrné hmotnosti 37,3 ± 3,0 kg, u kterých bylo provedeno vyšetření kapslovou endoskopií s boční optikou. Úvod vyšetření byl proveden v celkové anestezii, aktivovaná endoskopická kapsle byla zavedena do duodena gastroskopem. Po ukončení vyšetření byla endoskopická kapsle zachycena a data byla stažena a vyhodnocena. U všech zvířat byly hodnoceny endoskopické nálezy, tranzitní čas tenkým střevem, celková doba vyšetření a přehlednost sliznice v jednotlivých segmentech (duodenum, jejunum, ileum). Výsledky: Kapslová endoskopie byla provedena bez komplikací u všech zvířat. V jednom případě došlo k návratu a následné perzistenci kapsle v žaludku po celou dobu vyšetření. U zbylých pěti zvířat byl enteroskopický nález v normě. Závěr: Experimentální endoskopie pomocí kapsle s boční optikou je technicky proveditelná a představuje metodu vyšetření tenkého střeva vhodnou k použití v preklinických studiích. Enteroskopické nálezy získané touto metodou u experimentálního prasete jsou zcela srovnatelné s nálezy získanými u člověka.
Intoduction: Capsule endoscopy is a standard diagnostic method used especially in small bowel investigations. The main advantage is its non-invasiveness, both in clinical practice and in experimental settings. Aim: The aim of this pilot project was to develop a methodology to allow capsule endoscopy with a panoramic side view in an experimental pig and to verify this methodology in healthy animals. Methodology: A capsule endoscope with a side view was introduced into the duodenum of six healthy female pigs weighing 37.3 ± 3.0 kg using a gastroscope. The procedure was performed under general anesthesia. After the examination was completed, the endoscopic capsule was captured and the data were extracted and evaluated. Endoscopic findings, transit time through the small intestine, total examination time, and visibility of the small intestinal surface (duodenum, jejunum, and ileum) were evaluated in all animals. Results: Capsule endoscopy was performed without complications in all animals. In one case, the capsule returned and persisted in the stomach for the entire investigation. Enteroscopic findings were normal in all the remaining five animals. Conclusions: Capsule endoscopy is technically feasible. Endoscopic findings in the small intestine obtained by this method are comparable to those obtained in humans. The experimental capsule endoscopy results could provide an important basis for further preclinical projects.
- MeSH
- Gastrointestinal Tract diagnostic imaging MeSH
- Capsule Endoscopy * economics methods utilization MeSH
- Models, Animal MeSH
- Pilot Projects MeSH
- Sus scrofa * MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
- MeSH
- Balloon Enteroscopy methods trends utilization MeSH
- Biopsy methods trends utilization MeSH
- Crohn Disease diagnosis classification MeSH
- Duodenoscopy methods trends utilization MeSH
- Endoscopy, Digestive System * methods trends utilization MeSH
- Endoscopy, Gastrointestinal * methods instrumentation utilization MeSH
- Gastroscopy methods trends utilization MeSH
- Histological Techniques methods utilization MeSH
- Inflammatory Bowel Diseases * diagnosis classification therapy MeSH
- Capsule Endoscopy methods trends utilization MeSH
- Colonoscopy methods trends utilization MeSH
- Humans MeSH
- Postoperative Care methods utilization MeSH
- Statistics as Topic MeSH
- Colitis, Ulcerative diagnosis classification MeSH
- Check Tag
- Humans MeSH
- Publication type
- Review MeSH
- MeSH
- Azathioprine therapeutic use MeSH
- Cyclosporine therapeutic use MeSH
- Diverticulitis, Colonic * diagnosis surgery therapy MeSH
- Hepatitis C diagnosis therapy MeSH
- Adrenal Cortex Hormones therapeutic use MeSH
- Inflammatory Bowel Diseases * drug therapy MeSH
- Capsule Endoscopy utilization MeSH
- Drug Therapy, Combination MeSH
- Congresses as Topic MeSH
- Cryoglobulinemia * diagnosis MeSH
- Mercaptopurine therapeutic use MeSH
- Methotrexate therapeutic use MeSH
- Tumor Necrosis Factor-alpha antagonists & inhibitors therapeutic use MeSH
- Keywords
- období rigidní, semiflexibilní a flexibilní endoskopie, fibroskopie, videoendoskopie,
- MeSH
- History of Medicine * MeSH
- History, Modern 1601- MeSH
- Endoscopy history methods utilization MeSH
- Endoscopy, Gastrointestinal * history methods trends MeSH
- Capsule Endoscopy history methods utilization MeSH
- Laryngoscopy history methods utilization MeSH
- Humans MeSH
- Proctoscopy history methods utilization MeSH
- Ureteroscopy history methods utilization MeSH
- Check Tag
- Humans MeSH
- Keywords
- ER (endoskopická resekce), SK (screeningová kolonoskopie), TOKS (test na okultní krvácení do stolice), FS (flexibilní sigmoideoskopie),
- MeSH
- Capsule Endoscopy methods utilization MeSH
- Colonography, Computed Tomographic methods utilization MeSH
- Colonoscopy * methods instrumentation utilization MeSH
- Colorectal Surgery methods trends utilization MeSH
- Colorectal Neoplasms * diagnosis epidemiology prevention & control MeSH
- Quality of Health Care standards organization & administration trends MeSH
- Humans MeSH
- Evidence-Based Medicine MeSH
- Occult Blood MeSH
- Mass Screening * methods instrumentation utilization MeSH
- Preventive Medicine methods instrumentation trends MeSH
- Statistics as Topic MeSH
- Population Surveillance MeSH
- Government Programs methods organization & administration legislation & jurisprudence MeSH
- Check Tag
- Humans MeSH
- Geographicals
- Czech Republic MeSH
- Europe MeSH
- Keywords
- ER (endoskopická resekce), balonové enteroskopie, IPAA ileo-pouch-anální anastomóza (pouchoskopie), kolorektální karcinom u IBD, dispenzarizace nemocných s IBD,
- MeSH
- Biopsy MeSH
- Cholangiopancreatography, Endoscopic Retrograde methods trends utilization MeSH
- Crohn Disease diagnosis therapy MeSH
- Diagnostic Techniques, Digestive System trends utilization MeSH
- Diagnosis, Differential MeSH
- Dilatation methods utilization MeSH
- Endosonography methods instrumentation utilization MeSH
- Inflammatory Bowel Diseases * diagnosis etiology therapy MeSH
- Capsule Endoscopy methods utilization MeSH
- Colonoscopy * methods trends utilization MeSH
- Comorbidity MeSH
- Humans MeSH
- Prognosis MeSH
- Risk Factors MeSH
- Practice Guidelines as Topic MeSH
- Statistics as Topic MeSH
- Constriction, Pathologic diagnosis pathology therapy MeSH
- Pregnancy MeSH
- Colitis, Ulcerative MeSH
- Check Tag
- Humans MeSH
- Pregnancy MeSH
- Keywords
- refrakterní celiakie (RCD), RCDI, RCDII,
- MeSH
- Biopsy * methods utilization MeSH
- Celiac Disease * diagnosis complications mortality prevention & control MeSH
- Diet Therapy MeSH
- Diagnosis, Differential MeSH
- Double-Balloon Enteroscopy * trends utilization MeSH
- Endoscopy methods utilization MeSH
- Immunohistochemistry utilization MeSH
- Interleukin-15 antagonists & inhibitors administration & dosage pharmacology MeSH
- Capsule Endoscopy * trends utilization MeSH
- Humans MeSH
- Lymphoma diagnosis complications MeSH
- Multiplex Polymerase Chain Reaction utilization MeSH
- Pathological Conditions, Signs and Symptoms MeSH
- Malnutrition MeSH
- Prognosis MeSH
- Flow Cytometry utilization MeSH
- Research MeSH
- Check Tag
- Humans MeSH
- MeSH
- Biopsy methods utilization MeSH
- Celiac Disease * diagnosis diet therapy drug therapy complications prevention & control MeSH
- Double-Balloon Enteroscopy * trends utilization MeSH
- Endoscopy methods utilization MeSH
- Histological Techniques * methods utilization MeSH
- Capsule Endoscopy * trends utilization MeSH
- Humans MeSH
- Risk Factors MeSH
- Intestinal Neoplasms * diagnosis complications prevention & control MeSH
- Check Tag
- Humans MeSH
- MeSH
- Adenomatous Polyps diagnosis MeSH
- Biomedical Research MeSH
- Capsule Endoscopy * methods instrumentation utilization MeSH
- Colonoscopy methods utilization MeSH
- Colorectal Neoplasms * diagnosis MeSH
- Humans MeSH
- Multicenter Studies as Topic MeSH
- Mass Screening methods utilization MeSH
- Intestinal Polyps * diagnosis MeSH
- Prospective Studies MeSH
- Outcome and Process Assessment, Health Care MeSH
- Check Tag
- Humans MeSH
- Publication type
- Meeting Abstract MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- Geographicals
- Czech Republic MeSH
