- MeSH
- COVID-19 * prevention & control psychology MeSH
- Ethics MeSH
- Humans MeSH
- Local Government MeSH
- Socioeconomic Factors * MeSH
- Government MeSH
- Check Tag
- Humans MeSH
- Publication type
- Newspaper Article MeSH
- Keywords
- ústavní soud,
- MeSH
- Adult MeSH
- Doulas legislation & jurisprudence MeSH
- Humans MeSH
- Mothers legislation & jurisprudence MeSH
- Perinatal Death * MeSH
- Home Childbirth * ethics adverse effects legislation & jurisprudence MeSH
- Patient Rights ethics legislation & jurisprudence MeSH
- Liability, Legal MeSH
- Forensic Sciences legislation & jurisprudence MeSH
- Pregnancy MeSH
- Medical Neglect MeSH
- Women's Rights legislation & jurisprudence MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Pregnancy MeSH
- Female MeSH
- Publication type
- Case Reports MeSH
- Newspaper Article MeSH
- Legal Case MeSH
- Keywords
- porodní gauč,
- MeSH
- Humans MeSH
- Parturition psychology MeSH
- Hospitals, Maternity * trends supply & distribution MeSH
- Health Facility Environment organization & administration trends supply & distribution MeSH
- Pregnancy MeSH
- Interior Design and Furnishings * MeSH
- Check Tag
- Humans MeSH
- Pregnancy MeSH
- Female MeSH
- Publication type
- Newspaper Article MeSH
- MeSH
- Digital Health * trends MeSH
- Quality of Life MeSH
- Awards and Prizes * MeSH
- Patient Care instrumentation trends MeSH
- Publication type
- Newspaper Article MeSH
- Interview MeSH
- Keywords
- IKEM,
- MeSH
- Abdomen * surgery MeSH
- Humans MeSH
- Intestine, Small surgery MeSH
- Organ Transplantation * economics methods statistics & numerical data MeSH
- Patient Care Team MeSH
- Health Facilities statistics & numerical data MeSH
- Check Tag
- Humans MeSH
- Publication type
- Newspaper Article MeSH
- MeSH
- Immunity * MeSH
- Humans MeSH
- Primary Prevention * classification methods MeSH
- Sleep physiology immunology MeSH
- Hydrogen pharmacology therapeutic use MeSH
- Check Tag
- Humans MeSH
- Publication type
- Newspaper Article MeSH
- Review MeSH
Familial dysautonomia is a debilitating congenital neurodegenerative disorder with no causative therapy. It is caused by a homozygous mutation in ELP1 gene, resulting in the production of the transcript lacking exon 20. The compounds studied as potential treatments include the clinical candidate kinetin, a plant hormone from the cytokinin family. We explored the relationship between the structure of a set of kinetin derivatives (N = 72) and their ability to correct aberrant splicing of the ELP1 gene. Active compounds can be obtained by the substitution of the purine ring with chlorine and fluorine at the C2 atom, with a small alkyl group at the N7 atom, or with diverse groups at the C8 atom. On the other hand, a substitution at the N3 or N9 atoms resulted in a loss of activity. We successfully tested a hypothesis inspired by the remarkable tolerance of the position C8 to substitution, postulating that the imidazole of the purine moiety is not required for the activity. We also evaluated the activity of phytohormones from other families, but none of them corrected ELP1 mRNA aberrant splicing. A panel of in vitro ADME assays, including evaluation of transport across model barriers, stability in plasma and in the presence of liver microsomal fraction as well as plasma protein binding, was used for an initial estimation of the potential bioavailability of the active compounds. Finally, a RNA-seq data suggest that 8-aminokinetin modulates expression spliceosome components.
- MeSH
- Kinetin * pharmacology chemistry MeSH
- Humans MeSH
- Molecular Structure MeSH
- RNA Precursors * genetics metabolism MeSH
- RNA Splicing * drug effects MeSH
- Transcriptional Elongation Factors metabolism genetics MeSH
- Dose-Response Relationship, Drug MeSH
- Structure-Activity Relationship MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
OBJECTIVE: To assess the prevalence of pelvic floor and anal sphincter trauma in women after assisted vaginal delivery. METHODS: Retrospective study on 201 primiparous women after assisted vaginal delivery, control group 43 women after normal vaginal delivery. 4D translabial ultrasound examination of the levator ani and the anal sphincter was performed according to standard methodology at least 3 months postpartum. Ultrasound classification of trauma was performed by two independent evaluators blinded to clinical data. A third evaluator was asked to confirm findings in case of discrepancy. RESULTS: The LAM avulsion rate for normal delivery was 20.9%, for forceps 60%, for vacuum extraction 21.7% Odds ratio for forceps vs. NVD was 4.32 (1.69, 11.01), for vacuum vs. NVD 0.98 (0.409, 2.327). Ultrasound OASI rate was 33.3% for Forceps and 30.50% for Vacuum. This equated to an OR of 1.78 (0.85 - 3.10) for Forceps and 1.62 (0.85-3.10) for Vacuum relative to NVD. CONCLUSIONS: Our data confirm forceps as the major risk factor for levator avulsion. Forceps also implies a non-significantly higher risk of OASI compared to NVD.
- MeSH
- Anal Canal * injuries diagnostic imaging MeSH
- Adult MeSH
- Extraction, Obstetrical adverse effects MeSH
- Humans MeSH
- Young Adult MeSH
- Pelvic Floor * diagnostic imaging injuries MeSH
- Obstetrical Forceps adverse effects MeSH
- Retrospective Studies MeSH
- Pregnancy MeSH
- Ultrasonography * methods MeSH
- Vacuum Extraction, Obstetrical * adverse effects MeSH
- Delivery, Obstetric adverse effects methods MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Young Adult MeSH
- Pregnancy MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
