• MeSH
• beta 2-Microglobulin blood   MeSH
• Diagnosis, Differential methods   statistics & numerical data   MeSH
• Research Support as Topic MeSH
• Clinical Laboratory Techniques methods   statistics & numerical data   MeSH
• Humans MeSH
• Multiple Myeloma diagnosis   MeSH
• Biomarkers, Tumor MeSH
• Paraproteinemias diagnosis   MeSH
• Prognosis MeSH
• Check Tag
• Humans MeSH
• Publication type
• Review MeSH
• Comparative Study MeSH

Cíl studie: Standardizovat biochemická vyšetření v séru nemocných mnohočetným myelomem v souvislosti se zavedením nového mezinárodního prognostického indexu, který využívá jen dva laboratorní ukazatele, albumin a beta-2 mikroglobulin. Typ studie: Studie porovnává výsledky albuminu, beta-2 mikroglobulinu a částečně i koncentrace paraproteinu ze šesti hlavních spolupracujících center v rámci České myelomové skupiny, která koordinuje léčbu nemocných mnohočetným myelomem v ČR, za účelem harmonizace těchto vyšetření. Materiál a metody: K provedení studie byly vybrány laboratoře fakultních nemocnic – VFN Praha, Praha Vinohrady, FN Hradec Králové, FN Olomouc, FN Brno-Bohunice a FN Plzeň. Každý ze zasílaných vzorků sér byl rozdělen na 6 stejných dílů a zmražen při -80 °C. Transport vzorků do jednotlivých laboratoří byl proveden v chlazeném boxu a vzorky byly předány k uchování při nejméně –70 °C do dne stanovení. Celý projekt trval dva roky (2003–2005) a byl rozdělen na tři etapy. V první etapě byl standardizován albumin (metoda s bromkrezolovou zelení) a byla zjištěna nevhodnost stanovení beta-2 mikroglobulinu metodou RIA pro nesrovnatelnost získaných výsledků s ostatními metodami. Ve druhé etapě bylo standardizováno stanovení beta-2 mikroglobulinu, ale pro technické závady nebylo možné vzorky použít na stanovení koncentrace paraproteinů. Ve třetí etapě bylo rozesláno 12 vzorků sér vždy s jedním monoklonálním imunoglobulinem ke stanovení jeho koncentrace. Výsledky: Stanovení albuminu je dobře standardizováno, interval spolehlivosti pro 95 % se pohybuje mezi 5,9–6,1 % (toleranční limit je do 9 %). Metodickým sjednocením se podařilo dosáhnout srovnatelnosti výsledků stanovení beta-2 mikroglobulinu. Koeficient variace je ve všech stanoveních, kromě jednoho, do 15 % (toleranční limit je do 15,5 %). Stanovení koncentrace monoklonálních imunoglobulinů potvrdilo známou zkušenost, že toto stanovení nelze za stávajících metodik úplně sjednotit pro mnoho dílčích nejistých kroků. I přes tyto výhrady studie ukázala klinickou použitelnost a srovnatelnost výsledků z jednotlivých center u koncentrací nad 20 g/l. Závěr: Stanovení albuminu je dobře standardizováno a výsledky ze všech zúčastněných center jsou srovnatelné. Stanovení beta-2 mikroglobulinu, po metodickém sjednocení poskytuje srovnatelné výsledky bez významných rozdílů. Stanovení koncentrace monoklonálních imunoglobulinů poskytlo zejména ve vyšších koncentracích klinicky významných srovnatelné výsledky. Toto stanovení je nejvíce problematické, ale jde o sledování reaktivních změn každým pracovištěm, proto harmonizace výsledků není zatím aktuální ani reálná.

Objective: The standardization of the biochemical measurement procedures in a blood serum of patients with multiple myeloma concerning an implementation of a new international prognostic index, which uses only two laboratory markers, albumin and beta-2 microglobulin. Design: The study compares results of albumin, beta-2 microglobulin and partly the concentration of paraproteins from six cooperative centers, which provide treatment of the multiple myeloma in the Czech Republic in order to integrate these investigations. Material and Methods: The laboratories of university hospitals – General University Hospital of Prague, University Hospital of Prague – Vinohrady, Hradec Králové, Olomouc, Brno – Bohunice and Plzeň have been chosen for the implementation of the study. Each control serum sample was divided into six same parts and was frozen at -80 °C. The transportation of the samples to the single laboratory was performed in a frozen box and the samples were stored at least at -70 °C till the date of determination. The whole project lasted two years and step by step it was divided into three periods (2003–2005). In the first period the determination of albumin was standardized and the unsuitability of the RIA method determination of beta-2 microglobulin with other methods was proofed. In the second period the determination of beta-2 microglobulin was standardized successfully, but because of some technical defects it was not possible to use the samples for the determination of paraproteins concentration. In the third period of the study 12 samples of blood serums were distributed, always with one monoclonal immunoglobulin to determine its concentration. Results: The determination of albumin is well standardized, a confidence interval for 95% is between 5.9–6.1% (tolerance limit for external quality assessments up to 9%). The unification of methods managed a comparability of the results of beta-2 microglobulin. The variation coefficient is to the 15% (tolerance limit derived from biological variation of this analyte is up to 15.5%). The determination of monoclonal immunoglobulins concentration confirmed the known experience that it is impossible to consolidate the determination because of many partial uncertain steps. Nevertheless the study showed clinical usability and analytical comparability of the results from the single centers with the concentration of paraproteins over 20 g/l. Conclusion: The determination of albumin is well standardized and the results from all laboratories are comparable. The determination of beta-2 microglobulin after a methodical unification provides comparable results without any significant differences. The determination of the monoclonal immunoglobulins concentration provided comparable results especially in concentrations higher than 20 g/l. This determination is mostly questionable, but it is concerned to monitor reactive changes by every laboratory, therefore the integration of the results has not been actual so far.

• MeSH
• Clinical Chemistry Tests standards   utilization   MeSH
• Humans MeSH
• Multiple Myeloma classification   blood   MeSH
• Antibodies, Monoclonal analysis   classification   MeSH
• Reference Standards MeSH
• Check Tag
• Humans MeSH

• MeSH
• Cell Division MeSH
• Vascular Cell Adhesion Molecule-1 blood   MeSH
• Adult MeSH
• Factor Analysis, Statistical MeSH
• Clinical Laboratory Techniques MeSH
• Middle Aged MeSH
• Humans MeSH
• Intercellular Adhesion Molecule-1 blood   MeSH
• Multiple Myeloma diagnosis   etiology   MeSH
• Aged MeSH
• In Vitro Techniques MeSH
• Check Tag
• Adult MeSH
• Middle Aged MeSH
• Humans MeSH
• Male MeSH
• Aged MeSH
• Female MeSH
• Publication type
• Review MeSH
• Comparative Study MeSH

• MeSH
• beta 2-Microglobulin * blood   MeSH
• Diagnosis, Differential MeSH
• Immunoglobulin M analysis   MeSH
• Laboratories * standards   MeSH
• Humans MeSH
• Serum Albumin, Human * analysis   MeSH
• Multiple Myeloma * classification   blood   pathology   MeSH
• Antibodies, Monoclonal blood   MeSH
• Prognosis MeSH
• Reference Standards MeSH
• Reproducibility of Results MeSH
• Neoplasm Staging * standards   MeSH
• Check Tag
• Humans MeSH

• MeSH
• Biomarkers MeSH
• Interferon-beta therapeutic use   MeSH
• Clinical Laboratory Techniques methods   utilization   MeSH
• Humans MeSH
• Antibodies, Neutralizing * isolation & purification   MeSH
• Myxovirus Resistance Proteins * isolation & purification   MeSH
• Multiple Sclerosis, Relapsing-Remitting * drug therapy   MeSH
• Multiple Sclerosis drug therapy   MeSH
• Check Tag
• Humans MeSH

Reliability of testing in kinanthropology/kinesiology as well as in all behavioral and social sciences is a crucial moment of empirical research in these sciences. In physics its counterpart is precision of measurement. Contemporary situation in organization and unification in physical measurement unfortunately does not have a similar international movement in the reliability of testing. The article presents some fundamental analogies as well as differences between the measurement models in the „soft“ sciences and makes some projections and comparisons with the physical measurement. It is presented that the main conceptual background for linking physical-measurement replications and their „soft“ science parallel forms of a test is the general principle of randomness. However, there is a substantial difference in other conceptualization of the physical-measurement Gauss model and behavioral-measurement parallel-forms model the concepts of „precision“ and „reliability“ are mutually transferable into each other. One of main specificities of classical test theory model is that best estimate of the true (precise) value is influenced by regression toward the mean and therefore is not unbiased as it is in case of physical measurement.

• MeSH
• Accreditation methods   standards   utilization   MeSH
• Financing, Organized MeSH
• Calibration standards   MeSH
• Kinesiology, Applied methods   utilization   MeSH
• Clinical Laboratory Techniques standards   utilization   MeSH
• Humans MeSH
• Musculoskeletal Physiological Phenomena MeSH
• Movement physiology   MeSH
• Reference Values MeSH
• Models, Statistical MeSH
• Statistics as Topic MeSH
• Sports Medicine methods   MeSH
• Models, Theoretical MeSH
• Investigative Techniques MeSH
• Check Tag
• Humans MeSH

Cíle studie: Získat prvotní zkušenosti s alternativním odběrem vzorku (samoodběrem) pro testování přítomnosti HPV za účelem skríninku karcinomu děložního čípku. Typ studie: Původní práce. Název a sídlo pracoviště: Ústav molekulární a translační medicíny, LF UP a FN Olomouc. Metodika: Na základě předem projeveného zájmu o HPV vyšetření byly 215 ženám zaslány samoodběrové sady Evalyn? Brush. Cervikovaginální stěry obdržené poštou byly analyzovány v naší laboratoři na přítomnost HPV infekce systémem Cobas 4800 HPV (Roche) s následnou genotypizací pomocí PapilloCheck? HPV-Screening (Greiner Bio-One). Náhodně vybraným 60 ženám z našeho souboru byl společně se samodběrovou sadou zaslán dotazník zaměřený na jejich zkušenost se samoodběrem. Výsledky: Z rozeslaných 215 samoodběrových sad bylo k analýze doručeno 174 (81 %) cervikovaginálních stěrů. Všechny vzorky byly odebrány správně a bylo možné je vyšetřit Cobas 4800 HPV testem. PapilloCheck? HPV-Screening systémem bylo možné za zisku validního výsledku vyšetřit 98 % vzorků (171/174). Z vyšetřených 174 vzorků bylo 125 vzorků (72 %) HPV negativních, u 7 vzorků (4 %) byla detekována pouze přítomnost nízkorizikové HPV infekce a u 42 vzorků (24 %) jsme detekovali přítomnost vysokorizikové HPV infekce. Nejčastěji detekovanými vysoce rizikovými genotypy byly HPV 16 (11/42; 26 %) a HPV 53 (6/42; 14 %). V 10 případech byla detekována současná infekce několika hrHPV, v 5 z nich byla nalezena současně infekce lrHPV. Z 60 odeslaných dotazníků bylo zasláno zpět 48 (80 %). Z této skupiny ohodnotilo svou zkušenost se samoodběrovou sadou jako dobrou až výbornou 47 (98 %) dotázaných žen. Návod k použití Evalyn? Brush byl považován za dobrý až vynikající všemi ženami (100 %). Všechny ženy také ohodnotily použití samoodběrové sady jako dobré až vynikající. Podle očekávání většina žen z našeho souboru (n = 42 [88 %]) dává přednost samoodběru před odběrem vzorku lékařem. Závěr: Samoodběr cervikovaginálního stěru vede při použití dvou testovaných molekulárně genetických metod k validním výsledkům a byl českými ženami velmi dobře přijat. Zavedení samoodběru jako způsobu účasti na cervikálním skríninku by mohlo vést ke zvýšení návštěvnosti skríninkového programu karcinomu děložního čípku, a napomohlo by tak ke snížení incidence i mortality tohoto onemocnění v české populaci.

Objective: To get initial experience with alternative sampling (self-sampling) for HPV testing as the means of cervical cancer screening program. Design: Original work. Setting: Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University in Olomouc. Methods: Based on expression of interest, 215 self-sampling kits were posted to women. Evalyn? Brush Vaginal swabs obtained by self-sampling were analyzed for the presence of HPV infection by Cobas 4800 HPV (Roche) followed by genotyping using PapilloCheck? HPV-Screening (Greiner Bio-One). Sixty women randomly chosen from our sample were sent a questionnaire focused on their experience with self-sampling. Results: One hundred seventy-four of 215 (81%) distributed self-sampling devices have been delivered to analysis. All cervicovaginal swabs were sampled correctly and it was possible to analyze them by Cobas 4800 HPV test. Similarly, 98% (171/174) samples were analyzable by PapilloCheck? HPV-Screening. One hundred twenty-five (72%) of 174 tested samples were HPV negative. Low risk HPV infection was detected only in 7 samples (4%), and high risk HPV (hrHPV) infection was present in 42 samples (24%). The most frequently detected hrHPV genotypes were HPV16 (11/42; 26%) and HPV53 (6/42; 14%). HrHPV co-infection was detected in 10 cases, in 5 of them lrHPV infection was find also. Of the 60 questionnaires, 48 (80%) were returned. From this group, 47 (98%) women rated their experience with self-sampling device as good to excellent. User manual of self-sampling device was considered good to excellent by all women (100%). All women also rated the convenience of self-sampling device using as good to excellent. As expected, most of the women (n = 42 [88%]) preferred self-sampling to physician sampling. Conclusion: Cervicovaginal self-sampling leads to valid results of HPV screening using two molecular genetics methods and was accepted by Czech women very well. The self-sampling as an opportunity to participate in cervical cancer screening could increase the attendance of the screening program and would help to reduce the incidence and mortality for this disease in the Czech population.

• MeSH
• Self Administration methods   MeSH
• Early Detection of Cancer methods   instrumentation   MeSH
• Cervix Uteri virology   MeSH
• Genotyping Techniques MeSH
• Papillomavirus Infections * diagnosis   prevention & control   MeSH
• Clinical Laboratory Techniques * instrumentation   MeSH
• Humans MeSH
• Uterine Cervical Neoplasms diagnosis   prevention & control   MeSH
• Specimen Handling methods   MeSH
• Papillomaviridae * isolation & purification   MeSH
• Pilot Projects MeSH
• Mass Screening MeSH
• Reverse Transcriptase Polymerase Chain Reaction utilization   MeSH
• Prevalence MeSH
• Surveys and Questionnaires MeSH
• Patient Satisfaction MeSH
• Statistics as Topic MeSH
• Check Tag
• Humans MeSH
• Female MeSH
• Publication type
• Research Support, Non-U.S. Gov't MeSH

Pro ověření frekvence výskytu zdvojených paraproteinémií jsme analyzovali soubor 500 paraproteinémií, které jsme nově prokázali během 46 měsíců. Zdvojenou paraproteinémii, tj. takovou, u níž se paraproteiny liší typem lehkých řetězců, třídou těžkých řetězců imunoglobulinů nebo oběma těmito znaky, jsme prokázali celkem ve 27 pozorováních, tj. v 5,4% frekvenci. Z klinického hlediska šlo v 52 % o BGUS (biclonal gammopathy of undetermined significance), v 18 % o mnohočetný myelom a ve 30 % o jiné lymfoproliferativní onemocnění.

We analyzed group of 500 paraproteinemias for presence double paraproteinemias, which were newly proved in our laboratory during 46 months. Double paraproteinemias were confirmed in 27x (5.4 %) cases. They were in 52 % patients with BGUS (biclonal gammopathy of undetermined significance), in 18 % with multiple myeloma and in 30 % with other lymphoproliferative diseases.

• Keywords
• biklonální gamapatie, imunofixace,
• MeSH
• Electrophoresis MeSH
• Financing, Organized MeSH
• Immunoglobulins diagnostic use   isolation & purification   classification   MeSH
• Clinical Laboratory Techniques trends   utilization   MeSH
• Humans MeSH
• Multiple Myeloma MeSH
• Paraproteinemias immunology   classification   MeSH
• Check Tag
• Humans MeSH

We have prepared a candidate biocompatible construct for skin wound healing based on electrospun polycaprolactone (PCL) nanofibrous membranes. The membrane material was loaded either with L-arginine or with alaptide, or with a mixture of both bioactive components. Alaptide is a spirocyclic synthetic dipeptide, an analogue of melanocyte-stimulating hormone release-inhibiting factor. L-arginine is an amino acid with a basic guanidine side chain. It is a direct precursor of nitric oxide, which plays a pivotal role in skin repair. The presence and the distribution of the additives were proved with high-performance liquid chromatography, Fourier-transform infrared spectroscopy and Raman spectroscopy. The influence of L-arginine and alaptide on the morphology of the membrane was characterized using scanning electron microscopy. No statistically significant correlation between fiber diameter and drug concentration was observed. The membranes were then tested in vitro for their cytotoxicity, using primary human dermal fibroblasts, in order to obtain the optimal concentrations of the additives for in vivo tests in a rat model. The membranes with the highest concentration of L-arginine (10 wt. %) proved to be cytotoxic. The membranes with alaptide in concentrations from 0.1 to 2.5 wt.%, and with the other L-arginine concentrations (1 and 5 wt.%), did not show high toxicity. In addition, there was no observed improvement in cell proliferation on the membranes. The in vivo experiments revealed that membranes with 1.5 wt.% of alaptide or with 1.5 wt.% of alaptide in combination with 5 wt.% of L-arginine markedly accelerated the healing of skin incisions, and particularly the healing of skin burns, i.e. wounds of relatively large extent. These results indicate that our newly-developed nanofibrous membranes are promising for treating wounds with large damaged areas, where a supporting material is needed.

• MeSH
• Arginine chemistry   MeSH
• Biocompatible Materials chemistry   MeSH
• Peptides, Cyclic chemistry   MeSH
• Electrochemistry MeSH
• Electrodes MeSH
• Fibroblasts drug effects   MeSH
• Microscopy, Fluorescence MeSH
• Wound Healing drug effects   MeSH
• Rats MeSH
• Skin pathology   MeSH
• Drug Delivery Systems MeSH
• Humans MeSH
• Nanofibers chemistry   MeSH
• Neuropeptides chemistry   MeSH
• Peptides chemistry   MeSH
• Rats, Wistar MeSH
• Cell Proliferation MeSH
• Spectrum Analysis, Raman MeSH
• Spectroscopy, Fourier Transform Infrared MeSH
• In Vitro Techniques MeSH
• Materials Testing MeSH
• Tissue Engineering methods   MeSH
• Tissue Scaffolds chemistry   MeSH
• Chromatography, High Pressure Liquid MeSH
• Animals MeSH
• Check Tag
• Rats MeSH
• Humans MeSH
• Female MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH

Backgrounds: The current renaissance of the interest in prognostic factors (PF) in multiple myeloma (MM) is determined by the extended possibilities of intensive treatment regimes, which require individual stratification of patients with a need of independent highly predictive prognostic factors. Design and Subjects: The prognostic significance of 7 selected staging systems and some cytokines and adhesive molecules was evaluated in the group of 237 patients with MM treated between 1991-2002 by conventional therapy. Methods and results: The prognostic significance was assessed using survival curves (according to Kaplan-Meier) and log rank test (p<0.05). The prognostic meaning of the age was proved (with borderline for 65 years p=0.0005). The high predictive power (p=0.0000) with significant differences in overall survival medians of defined subgroups was found for Hb, S-creatinin, S-albumin, S-beta2microglobulin, S-thymidinkinase and plasmocytes count in the bone marrow. In the case of propidium-iodide index PI/CD138 (percentage of myeloma cells in S-phase of the cell cycle) with identification of plasmocytes using anti-CD138 monoclonal antibody the prognostic „turning point“ was set for the values of 3.0% with survival medians 48 vs. 15 months (p=0.0023). Serum levels of cytokines IL-6, IL-2 and their receptors, TNF-α and adhesive molecules VCAM-1 and ICAM-1 measured by ELISA method at the time point of MM diagnosis did not show any prognostic significance (p=0.15- 0.84), just the normal and increased sIL-6R serum levels predicted quite different medians of overall survival (30 vs. 11 months). Conclusions: This study proved the prognostic meaning of classical PF (age, Hb, creatinin, beta2-mikroglobulin and bone marrow plasmocytes) and also a very good predictive significance and practical clinical utility of PI/CD138 index and S-album

• Keywords
• jodidový proliferační index plazmocytů, cytoadhezivní molekuly,
• MeSH
• Survival Analysis MeSH
• Vascular Cell Adhesion Molecule-1 immunology   isolation & purification   MeSH
• Cytokines immunology   isolation & purification   MeSH
• Financing, Organized MeSH
• Clinical Laboratory Techniques methods   utilization   MeSH
• Humans MeSH
• Intercellular Adhesion Molecule-1 immunology   isolation & purification   MeSH
• Multiple Myeloma drug therapy   therapy   MeSH
• Plasma Cells immunology   pathology   transplantation   MeSH
• Prognosis MeSH
• Cell Proliferation MeSH
• Propidium MeSH
• Prospective Studies MeSH
• Antineoplastic Combined Chemotherapy Protocols standards   therapeutic use   MeSH
• Renal Insufficiency complications   MeSH
• Risk Factors MeSH
• Neoplasm Staging methods   utilization   MeSH
• Statistics as Topic MeSH
• Thymidine Kinase isolation & purification   adverse effects   MeSH
• Age Factors MeSH
• Check Tag
• Humans MeSH