Vojenské zdravotnické listy, ISSN 0372-7025 roč. 71, suppl. 1, December 2002
43 s. : tab., grafy ; 30 cm
- MeSH
- Bacterial Proteins MeSH
- Genome, Bacterial MeSH
- Communicable Diseases MeSH
- Cell Communication MeSH
- Microbiology MeSH
- Proteome MeSH
- Military Medicine MeSH
- Publication type
- Congress MeSH
- Conspectus
- Mikrobiologie
- NML Fields
- mikrobiologie, lékařská mikrobiologie
- infekční lékařství
- biologie
HU protein is a member of nucleoid-associated proteins (NAPs) and is an important regulator of bacterial virulence, pathogenesis and survival. NAPs are mainly DNA structuring proteins that influence several molecular processes by binding the DNA. HU ́s indispensable role in DNA-related processes in bacteria was described. HU protein is a necessary bacterial transcription factor and is considered to be a virulence determinant as well. Less is known about its direct role in host-pathogen interactions. The latest studies suggest that HU protein may be secreted outside bacteria and be a part of the extracellular matrix. Moreover, HU protein can be internalized in a host cell after bacterial infection. Its role in the host cell is not well described and further studies are extremely needed. Existing results suggest the involvement of HU protein in host cell immune response modulation in bacterial favor, which can help pathogens resist host defense mechanisms. A better understanding of the HU protein's role in the host cell will help to effective treatment development.
- MeSH
- Bacterial Infections microbiology physiopathology MeSH
- Cell Line microbiology MeSH
- Phagosomes * physiology microbiology MeSH
- Cell Fractionation methods MeSH
- Francisella tularensis * pathogenicity MeSH
- Host-Pathogen Interactions * physiology MeSH
- Cathepsin D isolation & purification MeSH
- Macrophages physiology microbiology MeSH
- Lysosomal-Associated Membrane Protein 1 isolation & purification MeSH
- rab GTP-Binding Proteins isolation & purification MeSH
- In Vitro Techniques MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
Mass spectrometry proteomics data are typically evaluated against publicly available annotated sequences, but the proteogenomics approach is a useful alternative. A single genome is commonly utilized in custom proteomic and proteogenomic data analysis. We pose the question of whether utilizing numerous different genome assemblies in a search database would be beneficial. We reanalyzed raw data from the exoprotein fraction of four reference Enterobacterial Repetitive Intergenic Consensus (ERIC) I-IV genotypes of the honey bee bacterial pathogen Paenibacillus larvae and evaluated them against three reference databases (from NCBI-protein, RefSeq, and UniProt) together with an array of protein sequences generated by six-frame direct translation of 15 genome assemblies from GenBank. The wide search yielded 453 protein hits/groups, which UpSet analysis categorized into 50 groups based on the success of protein identification by the 18 database components. Nine hits that were not identified by a unique peptide were not considered for marker selection, which discarded the only protein that was not identified by the reference databases. We propose that the variability in successful identifications between genome assemblies is useful for marker mining. The results suggest that various strains of P. larvae can exhibit specific traits that set them apart from the established genotypes ERIC I-V.
- MeSH
- Bacterial Proteins * genetics metabolism MeSH
- Databases, Protein MeSH
- Virulence Factors * genetics metabolism MeSH
- Genome, Bacterial * genetics MeSH
- Paenibacillus larvae * genetics pathogenicity metabolism MeSH
- Proteogenomics * methods MeSH
- Proteomics methods MeSH
- Bees microbiology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
Journal of clinical investigation. 12, ISSN 0021-9738 Supplement Vol. 100
S69 s. : il. ; 30 cm
The host-pathogen interaction represents a complex and dynamic biological system. The outcome of this interaction is dependent on the microbial pathogen properties to establish infection and the ability of the host to control infection. Although bacterial pathogens have evolved a variety of strategies to subvert host defense functions, several general mechanisms have been shown to be shared among these pathogens. As a result, host effectors that are critical for pathogen entry, survival and replication inside the host cells have become a new paradigm for antimicrobial targeting. This review focuses on the potential utility of a proteomics approach in defining the host-pathogen interaction from the host's perspective.
Cíl práce: Cílem této prospektivní multicentrické studie bylo stanovení incidence grampozitivních bakterií u infekcí krevního řečiště (IKR) a charakteristika jejich rezistence k antibiotikům v České republice. Materiál a metody: Studie probíhala na 15 pracovištích v České republice v období leden – duben 2003. Celkový počet grampozitivnimi bakteriemi vyvolaných IKR byl stanoven na 100, rozložených mezi 10 fakultních resp. Krajských center po 7 případech a 5 okresních center po 6 případech. Výsledky: Nejčastěji byly identifikovány kmeny Staphylococcus aureus (39 %), koaguláza-negativní stafylokoky (34 %), Streptococcus pneumoniae (11 %) a Enterococcus sp. (9 %). Rezistence k oxacilinu byla prokázána u 10 % kmenů Staphylococcus aureus a 41 % koaguláza-negativních stafylokoků. Všechny kmeny Streptococcus pneumoniae byly citlivé na penicilin a chloramfenikol. V případě enterokoku nebyly prokázány kmeny s rezistencí ke glykopeptidům. Produkce bakteriálního biofirmu byla prokázána u 22 (56 %) kmenů Staphylococcus aureus, 14 (42 %) koaguláza-negativních stafylokoků a 2 kmenů Enterococcus sp. Klinická významnost izolovaných grampozitivních bakterií je signifikantně podmíněna bakteriálním druhem (p = 0,01) a dosahuje nejvyšších hodnot u kmenů Streptococcus pneumoniae (100 %) a Staphylococcus aureus (87 %). V 32 % se jednalo o katetrové infekce a lze tedy potvrdit, že přítomnost umělého materiálu představuje významný rizikový faktor pro vznik IKR.
Aim of the study: The goal of this study was the determination of grampositive bacterial pathogens occurrence in bloodstream infections and antimicrobial susceptibility in the Czech Republic. Materials and methods: The study was organized in 15 hospitals in the Czech Repubhc from January to April 2003. In the whole, 100 evincible grampositive bloodstream infections, caused by grampositive pathogens, were included in the study. Results: The most frequently, the strains of Staphylococcus aureus (39 %), coagulase-negative staphylococci (34 %), Streptococcus pneumoniae (11 %) and Enterococcus sp. (9 %) were identified as etiologic agents. According to the results, resistence to oxacillin was determined in 10% Staphylococcus aureus strains and in 41 % coagulase-negative staphylococci. No Streptococcus pneumoniae strains were resistant to penicillin and chloramphenicol. All Enterococcus sp. strains were susceptible to vancomycin and teicoplanin. Production of bacterial biofilm was determined in 56 % Staphylococcus aureus strains and in 42 % coagulase-negative staphylococci. 100 % of Streptococcus pneumoniae strains and 87 % of Staphylococcus aureus strains were associated with bloodstream infections. The clinical importance of grampositive bacterial species significantly (p = 0.01) based on bacterial species. 32 % of bloodstream infections were approved as catheter-related infections and it can be concluded, that presence of catheter is important risk factor for occurrence of bloodstream infection.
- MeSH
- Anti-Bacterial Agents therapeutic use MeSH
- Drug Resistance, Microbial MeSH
- Escherichia coli pathogenicity MeSH
- Research Support as Topic MeSH
- Gram-Positive Bacterial Infections diagnosis drug therapy microbiology MeSH
- Blood Circulation MeSH
- Humans MeSH
- Microbial Sensitivity Tests MeSH
- Staphylococcus aureus pathogenicity MeSH
- Check Tag
- Humans MeSH
- Publication type
- Review MeSH
- Comparative Study MeSH
... D-Ala D-Ala carboxypeptidase DacD is involved in intracellular replication and it is necessary for bacterial ... ... Kollarcíková Miloslava - llleal and caecal microbiota of chickens - identification of new opportunistic pathogens ... ... Brno, CZ) -- 12:00- 12.25am Plzakova Lenka - Early cellular responses of germ free and specific pathogen ... ... break - Congress foyer -- 10:30- 10:55am Prokšová Magdaléna - Seeking host target of Francisella pathogenicity ...
54 nečíslovaných stran : barevné ilustrace ; 22 cm
- MeSH
- Host-Parasite Interactions MeSH
- Publication type
- Abstracts MeSH
- Congress MeSH
- Program MeSH
- Collected Work MeSH
- News MeSH
- Conspectus
- Patologie. Klinická medicína
- NML Fields
- infekční lékařství
INTRODUCTION: Red blood cells (RBCs), also known as erythrocytes, are underestimated in their role in the immune system. In mammals, erythrocytes undergo maturation that involves the loss of nuclei, resulting in limited transcription and protein synthesis capabilities. However, the nucleated nature of non-mammalian RBCs is challenging this conventional understanding of RBCs. Notably, in bony fishes, research indicates that RBCs are not only susceptible to pathogen attacks but express immune receptors and effector molecules. However, given the abundance of RBCs and their interaction with every physiological system, we postulate that they act in surveillance as sentinels, rapid responders, and messengers. METHODS: We performed a series of in vitro experiments with Cyprinus carpio RBCs exposed to Aeromonas hydrophila, as well as in vivo laboratory infections using different concentrations of bacteria. RESULTS: qPCR revealed that RBCs express genes of several inflammatory cytokines. Using cyprinid-specific antibodies, we confirmed that RBCs secreted tumor necrosis factor alpha (TNFα) and interferon gamma (IFNγ). In contrast to these indirect immune mechanisms, we observed that RBCs produce reactive oxygen species and, through transmission electron and confocal microscopy, that RBCs can engulf particles. Finally, RBCs expressed and upregulated several putative toll-like receptors, including tlr4 and tlr9, in response to A. hydrophila infection in vivo. DISCUSSION: Overall, the RBC repertoire of pattern recognition receptors, their secretion of effector molecules, and their swift response make them immune sentinels capable of rapidly detecting and signaling the presence of foreign pathogens. By studying the interaction between a bacterium and erythrocytes, we provide novel insights into how the latter may contribute to overall innate and adaptive immune responses of teleost fishes.
- MeSH
- Aeromonas hydrophila * immunology MeSH
- Cytokines * metabolism immunology MeSH
- Erythrocytes * immunology metabolism MeSH
- Phagocytosis immunology MeSH
- Gram-Negative Bacterial Infections * immunology MeSH
- Carps * immunology microbiology MeSH
- Fish Diseases * immunology microbiology MeSH
- Pathogen-Associated Molecular Pattern Molecules immunology MeSH
- Immunity, Innate MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
