- MeSH
- Electroencephalography MeSH
- Evoked Potentials MeSH
- Humans MeSH
- Learning MeSH
- Check Tag
- Humans MeSH
To monitor gradual changes in the replication foci distribution during early S phase, different segments of newly synthesized DNA were visualized by immunocytochemical mapping of two consecutively incorporated deoxythymidine analogs in pulse-chase-pulse experiments in HeLa cells. The resulting dual-labeled fluorescence images were evaluated using cross-correlation function (CCF) analysis. General changes of CCF shape due to image deterioration caused by blur, noise, and lateral sampling (pixel size) were also discussed. Using CCF analysis of model images simulating either random initiation of new replication foci, or the firing of new foci in close proximity to completed ones, we were able to ascribe the changes in the early S replication foci distribution to the latter mechanism. In contrast to the data published previously, we monitored the dynamics of all replication foci for up to 3 h. In addition, we showed that the replication foci dynamics is well described by random walk model, so that the average de-localization of individual foci is proportional to square root of the applied chase.
Aim: To compare nurses' and patients' reports of missed nursing care in surgical units. Design: A cross-sectional correlational study. Methods: The sample involved 130 nurses in surgical departments, and 112 patients assigned to nurses during various shifts were included. The Slovak version of the Missed Nursing Care Survey, the MISSCARE-Patient Survey, and the Generic Short Patient Experiences Questionnaire were used to collect data. Data were analyzed using descriptive and nonparametric inductive statistics. Results: The overall mean scores of the MISSCARE versions were low, suggesting a tendency for nurses and patients to perceive missed nursing care as a rarely occurring phenomenon. Patients' perceptions of specialist health care were positive, and only 20.7% of patients experienced any adverse events during hospitalization in surgical units. The nonsignificant relationship between nurses' and patients' reports of missed nursing care suggests differing perspectives on the amount and pattern of missed nursing care. A significant difference in the perception of missed nursing care in surgical wards was found among nurses but not among patients. A negative association between missed nursing care from the perspective of nurses and patients and various dimensions of patient-centered care was revealed. Conclusion: The study indicated a significant difference between nurses' and patients' perceptions and experiences of missed nursing care, highlighting the importance of including both perspectives.
In this work, we propose a new approach for three-dimensional registration of MR fractional anisotropy images with T1-weighted anatomy images of human brain. From the clinical point of view, this accurate coregistration allows precise detection of nerve fibers that is essential in neuroscience. A template matching algorithm combined with normalized cross-correlation was used for this registration task. To show the suitability of the proposed method, it was compared with the normalized mutual information-based B-spline registration provided by the Elastix software library, considered a reference method. We also propose a general framework for the evaluation of robustness and reliability of both registration methods. Both registration methods were tested by four evaluation criteria on a dataset consisting of 74 healthy subjects. The template matching algorithm has shown more reliable results than the reference method in registration of the MR fractional anisotropy and T1 anatomy image data. Significant differences were observed in the regions splenium of corpus callosum and genu of corpus callosum, considered very important areas of brain connectivity. We demonstrate that, in this registration task, the currently used mutual information-based parametric registration can be replaced by more accurate local template matching utilizing the normalized cross-correlation similarity measure.
- MeSH
- Algorithms MeSH
- Anisotropy MeSH
- Image Interpretation, Computer-Assisted methods MeSH
- Humans MeSH
- Longitudinal Studies MeSH
- Magnetic Resonance Imaging methods MeSH
- Brain Mapping methods MeSH
- Brain anatomy & histology MeSH
- Image Processing, Computer-Assisted methods MeSH
- Reference Values MeSH
- Reproducibility of Results MeSH
- Aged MeSH
- Software MeSH
- Imaging, Three-Dimensional methods MeSH
- Check Tag
- Humans MeSH
- Aged MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Vitamin D deficiency is a major health problem in the Saudi population. A negative association between blood pressure and vitamin D level has been suggested in several clinical and epidemiological studies and evidence for an effect of vitamin D in lowering blood pressure was reported. These findings indicate that 1,25(OH)2D3 deficiency may play a role in the pathogenesis of hypertension through its effect on the renin-angiotensin system. We are the first to investigate the correlation between blood pressure or renin concentration and vitamin D status in the Saudi population METHODS: we included 201 healthy Saudi premenopausal females (20-45 years old). Blood pressure was measured by a standardized method using an automated blood pressure monitor (BPTru). Fasting blood samples were collected from each participant after 20 minutes of rest in the seated position. Serum cholicalciferol, PTH and renin concentration were measured by sandwich chemiluminescence immunoassay method (DiaSorin, Italy). RESULTS: The analysis included 192 subjects who were normotensive (blood pressure <140/90 mmHg). A total of 34% of women had a severe deficiency (vitamin D ≤ 12.5 nmol/L); 41% had moderate deficiency (vitamin D levels between 12.5 - 25 nmol/L); 23% had mild deficiency (vitamin D level ≥ 25 - < 50 nmol/L); and 2% had insufficiency (vitamin D level ≥ 50 - ≤ 75 nmol/L). None had a sufficient level of ≥75 nmol/L. The systolic blood pressure ranged from 79-130.5 mmHg and the diastolic from 48.5-85.5 mmHg. Both systolic and diastolic blood pressures were significantly higher in women that were in the lower 2 quartiles of vitamin D. However, linear regression analysis adjusting for potential confounders showed that 25(OH)D level was not a predictor of either systolic or diastolic blood pressures. A negative correlation (although not significant) was found between vitamin D level and plasma renin concentration in this study group. CONCLUSION: Vitamin D deficiency was highly prevalent among the study group. Vitamin D was a not a predictor of either systolic or diastolic blood pressure. There was a negative correlation between vitamin D level and renin concentration although not statically significant. It is possible that any underlying relationship was obscured by the relatively young age group or due to the narrow blood pressure range of the studied population. A larger sample size including hypertensive subjects may be needed.
Aim: To determine the correlation between pharmacotherapy and high risk of fall based on the Morse Fall Scale (MFS score ≥45) in acute and long-term care settings. Design: Aquantitative cross-sectional descriptive correlation study. Methods:The study was conducted at a University Hospitalin Martin(UHM) anda selected long-term care facility (LTC) in Martin (Slovakia) June–October 2016. The pharmacotherapeutic data were obtained from the health documentation of the respondents. The fall risk was assessed by using the MFS screening tool within 24–48 hours of admission to the facility. Results:For the group of UHM patients (n = 63), the mean MFS score for fall risk was high (60.6 ± 22.4), and the correlation (p = 0.030) between the number of medications administered in 24 hours and high risk of fall was significant. For the group of LTCpatients (n = 89), the mean MFS score for fall risk was moderate (35.4 ± 15.9). Thecorrelations were not significant. Conclusion: Pharmacotherapy is animportant fall risk factor; therefore, it is necessary to determine it within the assessment of overall fall risk. The risk management of pharmacotherapy is an effective and important multifactorial intervention in programmes of fall prevention inacute and long-term care.
- Keywords
- Morse Fall Scale,
- MeSH
- Long-Term Care statistics & numerical data MeSH
- Drug Therapy * statistics & numerical data MeSH
- Risk Assessment methods statistics & numerical data MeSH
- Hospitalization statistics & numerical data MeSH
- Correlation of Data MeSH
- Middle Aged MeSH
- Humans MeSH
- Cross-Sectional Studies MeSH
- Risk Factors MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Accidental Falls * prevention & control MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Slovakia MeSH
Aim: The aim of the present study was to describe the association between primary healthcare (PHC) nurses' perceived stress and organizational culture and climate in a team context. Design:A descriptive, cross-sectional correlation study. Methods:29 teams in 18 PHC centers situated in one Lithuanian county participated in the study. A total of 187 nurses completed the Expanded Nursing Stress Scale, while 344 healthcare professionals (including nurses)responded to the Organizational Social Context questionnaire. Results:Absolute values of Spearman's rho correlation coefficients between 0.19–0.24 (p < 0.01)were found when examining the association of nurses' stress with organizational culture and climate across PHC teams. Whether the culture was resistant or proficient seemed to have associations with some of the stress factors experienced by nurses. Additionally, how the whole team reported stress or functionality in their organizational climate seemed to be associatedwith perceived stress of nurses. Conclusion:Investigating the organizational culture and climate experienced by PHC teams may help identify manageable problems and decrease stress experienced by nurses. The study also allows the possibility of nursing scholars using the study's designs and instruments for further investigation of teams.
- MeSH
- Correlation of Data MeSH
- Humans MeSH
- Occupational Stress * MeSH
- Health Facility Environment organization & administration statistics & numerical data MeSH
- Cross-Sectional Studies MeSH
- Surveys and Questionnaires MeSH
- Statistics as Topic MeSH
- Nurses * psychology MeSH
- Check Tag
- Humans MeSH
- Geographicals
- Lithuania MeSH
